breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 49,765 C→T intergenic (+134/‑58) kefC → / → folA glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase
RA 49,884 C→T P21L (CCG→CTG)  folA → type 3 dihydrofolate reductase
RA 214,743 A→C Y174D (TAC→GAC)  proS ← proline‑‑tRNA ligase
MC JC 2,399,465 Δ7 bp coding (650‑656/939 nt) lrhA ← transcriptional regulator LrhA
RA 3,754,231 G→T R127S (CGC→AGC)  selA ← L‑seryl‑tRNA(Sec) selenium transferase
RA 4,267,454 C→T R20C (CGC→TGC)  soxR → redox‑sensitive transcriptional activator SoxR

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 356981 357568 588 2 [0] [1] 2 lacA galactoside O‑acetyltransferase
* * ÷ NZ_CP009273 380269 380289 21 2 [1] [1] 2 BW25113_RS01880/tauA PIG‑L family deacetylase/taurine ABC transporter substrate‑binding protein
* * ÷ NZ_CP009273 524158 524205 48 2 [1] [1] 2 BW25113_RS24960 hypothetical protein
* * ÷ NZ_CP009273 716403 716423 21 2 [0] [0] 2 BW25113_RS26090/kdpE leader peptide SpeFL/two‑component system response regulator KdpE
* * ÷ NZ_CP009273 766536 766588 53 2 [1] [1] 6 mngB/cydA mannosylglycerate hydrolase/cytochrome ubiquinol oxidase subunit I
* * ÷ NZ_CP009273 772577 772589 13 2 [0] [1] 2 tolA cell envelope integrity protein TolA
* * ÷ NZ_CP009273 982952 982984 33 2 [1] [0] 2 ompF/asnS porin OmpF/asparagine‑‑tRNA ligase
* * ÷ NZ_CP009273 1032599 1032608 10 2 [0] [1] 4 hyaF hydrogenase expression/formation protein
* * ÷ NZ_CP009273 1193181 1193252 72 2 [1] [1] 2 BW25113_RS05920 hypothetical protein
* * ÷ NZ_CP009273 1207571 1207614 44 2 [1] [0] 2 iraM/BW25113_RS25985 anti‑adapter protein IraM/protein YmgK
* * ÷ NZ_CP009273 1294935 1294979 45 3 [0] [1] 2 BW25113_RS25865 hypothetical protein
* * ÷ NZ_CP009273 1459434 1459536 103 2 [1] [1] 2 [BW25113_RS25580] [BW25113_RS25580]
* * ÷ NZ_CP009273 1540358 1540506 149 2 [1] [1] 2 yddL/yddG porin/aromatic amino acid efflux DMT transporter YddG
* * ÷ NZ_CP009273 1632026 1632034 9 2 [1] [0] 2 gnsB addiction module toxin, GnsA/GnsB family
* * ÷ NZ_CP009273 1663836 1663889 54 4 [1] [1] 2 [ynfL] [ynfL]
* * ÷ NZ_CP009273 2102762 2102777 16 2 [1] [1] 4 wzx O16 family O‑antigen flippase
* * ÷ NZ_CP009273 2379234 2379266 33 2 [1] [1] 2 yfbK/yfbL VWA domain‑containing protein/M28 family metallopeptidase
* * ÷ NZ_CP009273 2380368 2380380 13 2 [1] [0] 2 yfbL/yfbM M28 family metallopeptidase/YfbM family protein
* * ÷ NZ_CP009273 2449367 2449397 31 2 [1] [1] 3 yfcV/sixA fimbrial protein/phosphohistidine phosphatase SixA
* * ÷ NZ_CP009273 2622985 2623077 93 2 [1] [1] 2 yfgG/yfgH YfgG family protein/glycine zipper 2TM domain‑containing protein
* * ÷ NZ_CP009273 2753673 2753715 43 2 [1] [1] 2 yfjI YfjI family protein
* * ÷ NZ_CP009273 2899026 2899068 43 2 [1] [1] 2 [BW25113_RS14445] [BW25113_RS14445]
* * ÷ NZ_CP009273 2975712 2975773 62 2 [1] [1] 2 araE/kduD arabinose‑proton symporter AraE/2‑dehydro‑3‑deoxy‑D‑gluconate 5‑dehydrogenase KduD
* * ÷ NZ_CP009273 2988641 2988665 25 2 [1] [1] 2 BW25113_RS14850/BW25113_RS26010 response regulator transcription factor/hypothetical protein
* * ÷ NZ_CP009273 3262697 3262769 73 2 [1] [1] 2 yhaC YhaC family protein
* * ÷ NZ_CP009273 3354280 3354290 11 3 [0] [1] 3 gltD/gltF glutamate synthase subunit GltD/DUF1120 domain‑containing protein
* * ÷ NZ_CP009273 3721235 3721235 1 2 [0] [0] 2 BW25113_RS18495/xylB inner membrane protein YiaB/xylulokinase

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 = 287112NA (NA)24 (1.160) 19/166 0.1 88.9% intergenic (‑55/‑99) BW25113_RS01405/BW25113_RS01410 IS1‑like element IS1B family transposase/hypothetical protein
?NZ_CP009273 481716 = 3 (0.140)coding (500/648 nt) acrR multidrug efflux transporter transcriptional repressor AcrR
* ? NZ_CP009273 360104 =NA (NA)39 (1.880) 21/166 0.1 47.6% intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 43 (2.070)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 = 4817243 (0.140)15 (0.720) 12/166 0.5 83.3% coding (508/648 nt) acrR multidrug efflux transporter transcriptional repressor AcrR
?NZ_CP009273 = 1046001 NA (NA)intergenic (+15/+418) BW25113_RS05135/cspH IS1‑like element IS1D family transposase/cold shock‑like protein CspH
* ? NZ_CP009273 1203246 =6 (0.290)14 (0.840) 11/134 0.3 74.0% coding (290/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 1205075 = 5 (0.300)coding (40/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 12032616 (0.290)21 (1.250) 15/134 0.1 81.0% coding (305/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 = 1205058 5 (0.300)coding (57/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 1391494NA (NA)13 (0.630) 7/164 1.0 100% intergenic (+145/‑128) BW25113_RS06980/ynaJ IS5‑like element IS5 family transposase/DUF2534 family protein
?NZ_CP009273 1972747 = 0 (0.000)intergenic (‑293/‑487) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
* ? NZ_CP009273 = 19727510 (0.000)31 (1.490) 21/166 0.1 100% intergenic (‑297/‑483) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
?NZ_CP009273 = 2283592 NA (NA)intergenic (‑68/‑387) BW25113_RS11435/narP IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP