breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 32 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,152,7270TG61.5% 5.3 / 12.7 13G165G (GGT→GGGycfHmetal‑dependent hydrolase
*NZ_CP009273807,1770TG59.1% 22.5 / 26.2 22V67G (GTG→GGG) bioCmalonyl‑ACP O‑methyltransferase BioC
*NZ_CP0092734,594,1610CT58.5% ‑1.1 / 43.6 41S62F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ
*NZ_CP009273822,9550TG58.3% 3.7 / 10.4 12T495P (ACC→CCC) ybhFATP‑binding cassette domain‑containing protein
*NZ_CP0092731,024,8120TG58.3% 3.8 / 12.4 12Y205S (TAC→TCC) rlmI23S rRNA (cytosine(1962)‑C(5))‑methyltransferase RlmI
*NZ_CP009273765,6750AC57.9% 15.0 / 18.0 19T748P (ACC→CCC) mngBmannosylglycerate hydrolase
*NZ_CP0092731,152,7450AG57.1% 4.2 / 14.4 14G171G (GGA→GGGycfHmetal‑dependent hydrolase
*NZ_CP0092734,213,5250CT57.1% ‑3.4 / 12.3 14R11K (AGA→AAA) iclRglyoxylate bypass operon transcriptional repressor IclR
*NZ_CP0092731,340,9100CA56.2% 9.7 / 23.9 16L30F (TTG→TTTpdeRcyclic di‑GMP phosphodiesterase
*NZ_CP0092732,089,5670TG56.2% 9.7 / 17.3 16G81G (GGT→GGGhisFimidazole glycerol phosphate synthase subunit HisF
*NZ_CP0092734,594,1670CT56.1% 10.9 / 46.4 41S64F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ
*NZ_CP0092732,794,4960TG53.8% 7.3 / 12.8 13V58G (GTG→GGG) nrdIclass Ib ribonucleoside‑diphosphate reductase assembly flavoprotein NrdI
*NZ_CP0092734,527,9260AC53.8% 6.9 / 15.2 13G219G (GGT→GGGnanMN‑acetylneuraminate epimerase
*NZ_CP009273510,0740TC53.3% 15.6 / 20.2 15E81G (GAA→GGA) ybbJNfeD family protein
*NZ_CP0092732,089,5710AG53.3% 3.9 / 15.0 15I83V (ATT→GTT) hisFimidazole glycerol phosphate synthase subunit HisF
*NZ_CP0092732,193,6610TG53.3% 14.8 / 16.7 15pseudogene (3709/3796 nt)BW25113_RS25680WGR and DUF4132 domain‑containing protein
*NZ_CP0092733,941,2100CT53.3% 3.8 / 18.1 15E40K (GAA→AAA) hdfRHTH‑type transcriptional regulator HdfR
*NZ_CP0092733,963,4170AC53.3% 8.7 / 11.0 15T343P (ACC→CCC) wzzEECA polysaccharide chain length modulation protein
*NZ_CP009273297,0750AC52.9% 20.0 / 23.1 17V175G (GTG→GGG) paoBaldehyde oxidoreductase FAD‑binding subunit PaoB
*NZ_CP009273443,1610TA52.9% 9.5 / 21.0 17M1M (ATG→TTG) †cyoEheme o synthase

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 454243 =24 (1.160)5 (0.250) 5/160 1.4 17.8% intergenic (+87/‑101) clpX/lon ATP‑dependent protease ATP‑binding subunit ClpX/endopeptidase La
?NZ_CP009273 = 604803 NA (NA)intergenic (+170/+112) BW25113_RS03025/entD IS4‑like element IS421 family transposase/enterobactin synthase subunit EntD