breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 49,736 A→T intergenic (+105/‑87) kefC → / → folA glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase
RA 108,362 A→G F242S (TTC→TCC)  zapD ← cell division protein ZapD
RA 115,488 T→C F90S (TTC→TCC)  ampD → 1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase AmpD
RA 115,628 A→C T137P (ACG→CCG)  ampD → 1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase AmpD
RA 139,618 A→G E118G (GAA→GGA)  yadG → ABC transporter ATP‑binding protein
RA 209,716 T→C I300T (ATC→ACC)  tilS → tRNA lysidine(34) synthetase TilS
RA 221,335 T→C noncoding (1078/1542 nt) BW25113_RS01010 → 16S ribosomal RNA
RA 232,840 T→A V96E (GTG→GAG)  dnaQ → DNA polymerase III subunit epsilon
RA 264,689 (A)5→4 intergenic (‑18/+311) ykfA ← / ← perR GTPase family protein/LysR substrate‑binding domain‑containing protein
RA 297,509 G→C G30G (GGC→GGG paoB ← aldehyde oxidoreductase FAD‑binding subunit PaoB
RA 314,035 A→G intergenic (‑5/+7) rclC ← / ← rclB reactive chlorine species resistance protein RclC/reactive chlorine resistance periplasmic protein RclB
RA 340,444 (A)5→4 coding (558/816 nt) BW25113_RS01680 → hypothetical protein
RA 354,025 T→C E126G (GAA→GGA)  cynR ← transcriptional regulator CynR
RA 386,045 T→C pseudogene (339/1527 nt) BW25113_RS01915 → outer membrane autotransporter barrel domain‑containing protein
RA 465,464 T→C S380P (TCG→CCG)  mdlA → SmdA family multidrug ABC transporter permease/ATP‑binding protein
RA 508,601 (C)6→7 coding (570/1293 nt) ybaT → APC family permease
RA 510,152 Δ1 bp coding (164/459 nt) ybbJ ← NfeD family protein
RA 539,975 A→G D258D (GAT→GAC allC ← allantoate deiminase
RA 551,422 C→G G452G (GGC→GGG cysS → cysteine‑‑tRNA ligase
RA 554,427 A→G intergenic (+217/‑3) fimA → / → fimC type 1 fimbrial major subunit FimA/type 1 fimbria chaperone FimC
RA 579,943 (T)8→7 intergenic (+57/+193) appY → / ← ompT DNA‑binding transcriptional activator AppY/omptin family outer membrane protease OmpT
RA 601,986 A→G F285S (TTC→TCC)  ybdK ← YbdK family carboxylate‑amine ligase
RA 661,892 A→C G594G (GGT→GGG mrdA ← peptidoglycan DD‑transpeptidase MrdA
RA 674,442 T→G T141P (ACT→CCT)  ybeT ← tetratricopeptide repeat protein
RA 769,756 A→C E35D (GAA→GAC cydX → cytochrome bd‑I oxidase subunit CydX
RA 802,549 T→G T63T (ACA→ACC ybhC ← putative acyl‑CoA thioester hydrolase
RA 860,269 A→G V106A (GTC→GCC)  moeB ← molybdopterin‑synthase adenylyltransferase MoeB
RA 950,036 (T)5→4 intergenic (‑114/+292) focA ← / ← ycaO formate transporter FocA/30S ribosomal protein S12 methylthiotransferase accessory factor YcaO
RA 951,809 T→G T94P (ACC→CCC)  ycaO ← 30S ribosomal protein S12 methylthiotransferase accessory factor YcaO
RA 1,054,234 A→G E232G (GAA→GGA)  torC → pentaheme c‑type cytochrome TorC
RA 1,060,988 (A)6→5 intergenic (+240/‑53) yccE → / → agp YccE family protein/bifunctional glucose‑1‑phosphatase/inositol phosphatase
RA 1,065,426 T→G T129P (ACC→CCC)  rutF ← pyrimidine utilization flavin reductase protein F
RA 1,076,727 (T)5→4 intergenic (+458/‑85) putP → / → BW25113_RS05295 sodium/proline symporter PutP/FTR1 family protein
RA 1,110,952 T→C intergenic (‑6/+166) mdtG ← / ← lpxL multidrug efflux MFS transporter MdtG/kdo(2)‑lipid IV(A) lauroyltransferase
RA 1,122,270 (A)6→5 coding (9/924 nt) yceM → Gfo/Idh/MocA family oxidoreductase
RA 1,194,248 A→G D33G (GAC→GGC)  lit → cell death peptidase Lit
RA 1,259,173 A→G T2A (ACC→GCC)  hemA → glutamyl‑tRNA reductase
RA 1,268,851 T→C T69A (ACC→GCC)  ychN ← DsrE/F sulfur relay family protein YchN
RA 1,276,251 Δ1 bp coding (932/3744 nt) narG → nitrate reductase subunit alpha
RA 1,300,524 Δ1 bp coding (504/1005 nt) oppF → murein tripeptide/oligopeptide ABC transporter ATP‑binding protein OppF
RA 1,321,615 T→C F169F (TTT→TTC rluB → 23S rRNA pseudouridine(2605) synthase RluB
RA 1,331,016 A→G Y310C (TAT→TGT)  acnA → aconitate hydratase AcnA
RA 1,392,826 T→C Q18Q (CAA→CAG uspE ← universal stress protein UspE
RA 1,394,163 G→A L111L (CTG→TTG)  ogt ← methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase
RA 1,402,000 A→G I18T (ATC→ACC)  dgcM ← diguanylate cyclase DgcM
RA 1,417,788 (C)6→7 coding (69/120 nt) BW25113_RS26130 ← hypothetical protein
RA 1,423,602 T→C R99R (CGT→CGC stfR → prophage tail fiber N‑terminal domain‑containing protein
RA 1,434,377 A→G F222S (TTC→TCC)  nifJ ← pyruvate:ferredoxin (flavodoxin) oxidoreductase
RA 1,454,774 A→G D16G (GAT→GGT)  paaI → hydroxyphenylacetyl‑CoA thioesterase PaaI
RA 1,499,566 A→G S258P (TCC→CCC)  ydcO ← BenE family transporter YdcO
RA 1,503,965 T→C D67D (GAT→GAC hicB → type II toxin‑antitoxin system antitoxin HicB
RA 1,505,430 A→G K391E (AAA→GAA)  ydcR → PLP‑dependent aminotransferase family protein
RA 1,531,275 G→A L98L (CTG→TTG)  narW ← nitrate reductase molybdenum cofactor assembly chaperone
RA 1,547,935 A→G V54A (GTA→GCA)  adhP ← alcohol dehydrogenase AdhP
RA 1,558,439 A→G Y518H (TAT→CAT)  dosP ← oxygen‑sensing cyclic‑di‑GMP phosphodiesterase
RA 1,568,198 A→G F417S (TTC→TCC)  pqqL ← insulinase family protein
RA 1,573,157 A→T I148N (ATC→AAC)  yddA ← ABC transporter ATP‑binding protein/permease
RA 1,575,825 A→T D319E (GAT→GAA ydeN ← sulfatase
RA 1,576,388 A→G S132P (TCC→CCC)  ydeN ← sulfatase
RA 1,579,109 A→C G545G (GGT→GGG ydeP ← acid resistance putative oxidoreductase YdeP
RA 1,613,753 (A)6→5 coding (377/435 nt) marR → multiple antibiotic resistance transcriptional regulator MarR
RA 1,632,437 (T)7→6 intergenic (‑71/+8) BW25113_RS08135 ← / ← BW25113_RS25620 hypothetical protein/hypothetical protein
RA 1,634,151 Δ1 bp coding (164/534 nt) rrrQ ← lysozyme
RA 1,659,576 T→C F14S (TTC→TCC)  clcB → voltage‑gated ClC‑type chloride channel ClcB
RA 1,662,649 T→G H58P (CAC→CCC)  mlc ← DNA‑binding transcriptional repressor Mlc
RA 1,671,899 T→G T88P (ACG→CCG)  pntA ← Re/Si‑specific NAD(P)(+) transhydrogenase subunit alpha
RA 1,673,542 A→G K287E (AAG→GAG)  ydgH → DUF1471 family protein YdgH
RA 1,717,498 G→C G374A (GGC→GCC)  ydhK → FUSC family protein
RA 1,782,276 T→G V192G (GTG→GGG)  ppsR → posphoenolpyruvate synthetase regulatory kinase/phosphorylase PpsR
RA 1,803,201 A→G V97A (GTC→GCC)  yniB ← YniB family protein
RA 1,807,028 T→C E121G (GAA→GGA)  BW25113_RS09050 ← hypothetical protein
RA 1,812,807 (C)7→8 intergenic (‑50/+55) celF ← / ← chbR 6‑phospho‑beta‑glucosidase/transcriptional regulator ChbR
RA 1,838,813 T→C I106V (ATT→GTT)  ynjI ← DUF1266 domain‑containing protein
RA 1,854,126 T→C M121V (ATG→GTG)  ydjK ← MFS transporter
RA 1,903,061 A→T intergenic (‑38/‑121) mgrB ← / → yobH PhoP/PhoQ regulator MgrB/YobH family protein
RA 1,965,782 T→G E389D (GAA→GAC tar ← methyl‑accepting chemotaxis protein II
RA 1,980,420 A→G G5G (GGA→GGG ftnB → non‑heme ferritin‑like protein
RA 2,003,480 T→C F60S (TTC→TCC)  yedK → SOS response‑associated peptidase
RA 2,038,183 (T)5→4 intergenic (+78/‑236) BW25113_RS10330 → / → yeeJ tRNA‑Asn/inverse autotransporter adhesin YeeJ
RA 2,057,664 T→C G95G (GGA→GGG cobT ← nicotinate‑nucleotide‑‑dimethylbenzimidazole phosphoribosyltransferase
RA 2,064,259 T→G V93G (GTG→GGG)  BW25113_RS10440 → GTPase family protein
RA 2,107,964 T→C intergenic (‑156/+19) galF ← / ← wcaM UTP‑‑glucose‑1‑phosphate uridylyltransferase GalF/colanic acid biosynthesis protein WcaM
RA 2,131,004 A→G intergenic (‑280/‑379) wza ← / → yegH polysaccharide export protein Wza/TerC family protein
RA 2,176,805 T→G D109A (GAC→GCC)  yegX ← glycoside hydrolase family 25 protein
RA 2,220,044 A→G S236P (TCG→CCG)  yohF ← SDR family oxidoreductase
RA 2,229,956 A→G K405E (AAA→GAA)  preA → NAD‑dependent dihydropyrimidine dehydrogenase subunit PreA
RA 2,283,874 Δ1 bp intergenic (‑350/‑105) BW25113_RS11435 ← / → narP IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP
RA 2,284,519 T→C S181P (TCC→CCC)  narP → nitrate/nitrite response regulator protein NarP
RA 2,345,414 T→G H147P (CAC→CCC)  glpT ← glycerol‑3‑phosphate transporter
RA 2,396,099 G→C A413G (GCC→GGC)  nuoC ← NADH‑quinone oxidoreductase subunit C/D
MC JC 2,399,465 Δ7 bp coding (650‑656/939 nt) lrhA ← transcriptional regulator LrhA
RA 2,410,868 A→T G103G (GGA→GGT yfcC → putative basic amino acid antiporter YfcC
RA 2,416,814 T→C K69E (AAA→GAA)  rpnB ← recombination‑promoting nuclease RpnB
RA 2,425,778 T→C E215G (GAA→GGA)  folC ← bifunctional tetrahydrofolate synthase/dihydrofolate synthase
RA 2,439,101 T→C K255E (AAG→GAG)  mepA ← penicillin‑insensitive murein endopeptidase
RA 2,463,070 T→C F154S (TTC→TCC)  BW25113_RS12270 → hypothetical protein
RA 2,505,777 T→C I137V (ATC→GTC)  mntH ← Nramp family divalent metal transporter
RA 2,526,050 A→G T95A (ACC→GCC)  cysK → cysteine synthase A
RA 2,536,106 T→C E261G (GAA→GGA)  cysP ← thiosulfate/sulfate ABC transporter substrate‑binding protein CysP
RA 2,554,121 A→C N169T (AAC→ACC)  BW25113_RS12740 → hypothetical protein
RA 2,661,508 T→G T250P (ACC→CCC)  hcaR ← DNA‑binding transcriptional regulator HcaR
RA 2,678,723 A→C G48G (GGT→GGG glyA ← serine hydroxymethyltransferase
RA 2,726,562 (T)5→4 coding (971/2574 nt) clpB ← ATP‑dependent chaperone ClpB
RA 2,739,096 T→C E61G (GAA→GGA)  rimM ← ribosome maturation factor RimM
RA 2,741,778 A→T Y153F (TAC→TTC)  ypjD → inner membrane protein YpjD
RA 2,751,237 A→G V241V (GTT→GTC BW25113_RS13685 ← hypothetical protein
RA 2,778,934 (T)6→5 intergenic (‑564/+187) BW25113_RS25705 ← / ← BW25113_RS13825 DUF5507 domain‑containing protein/tRNA‑Ile
RA 2,801,112 A→C T208P (ACC→CCC)  proX → glycine betaine/L‑proline ABC transporter substrate‑binding protein ProX
RA 2,818,451 T→C E162G (GAA→GGA)  mltB ← lytic murein transglycosylase B
RA 2,859,998 T→C I305V (ATT→GTT)  rpoS ← RNA polymerase sigma factor RpoS
RA 2,882,413 A→C V349G (GTT→GGT)  cysI ← assimilatory sulfite reductase (NADPH) hemoprotein subunit
RA 2,888,711 A→G V143A (GTC→GCC)  ygcQ ← electron transfer flavoprotein subunit alpha/FixB family protein
RA 2,982,555 T→C F232S (TTC→TCC)  yqeI → transcriptional regulator
RA 2,987,546 T→C I83I (ATT→ATC BW25113_RS14845 → lytic transglycosylase domain‑containing protein
RA 3,011,010 A→G E531G (GAA→GGA)  ygfK → putative selenate reductase subunit YgfK
RA 3,098,336 Δ1 bp coding (545/1080 nt) mltC → membrane‑bound lytic murein transglycosylase MltC
RA 3,103,871 (A)7→6 intergenic (+71/+78) BW25113_RS15410 → / ← yghD tRNA‑Phe/GspM family type II secretion system protein YghD
RA 3,273,721 A→G E49G (GAA→GGA)  agaV → PTS N‑acetylgalactosamine transporter subunit IIB
RA 3,291,696 T→G L170F (TTA→TTC yraR ← NAD(P)H‑binding protein
RA 3,458,386 A→G E369G (GAA→GGA)  gspL → type II secretion system protein GspL
RA 3,535,339 T→C F525S (TTC→TCC)  feoB → Fe(2+) transporter permease subunit FeoB
RA 3,536,347 A→G intergenic (+24/‑179) feoC → / → rpnA [Fe‑S]‑dependent transcriptional repressor FeoC/recombination‑promoting nuclease RpnA
RA 3,576,634 A→G R79R (CGA→CGG BW25113_RS17825 → YrhA family protein
RA 3,579,168 T→C E339G (GAA→GGA)  ggt ← gamma‑glutamyltransferase
RA 3,642,054 A→G E56G (GAA→GGA)  arsR → As(III)‑sensing metalloregulatory transcriptional repressor ArsR
RA 3,646,989 (A)5→4 intergenic (+23/‑332) BW25113_RS18165 → / → slp hypothetical protein/outer membrane lipoprotein Slp
RA 3,717,969 T→C E237G (GAA→GGA)  glyQ ← glycine‑‑tRNA ligase subunit alpha
RA 3,786,467 T→C I193V (ATT→GTT)  yibB ← protein YibB
RA 3,786,991 T→C D18G (GAT→GGT)  yibB ← protein YibB
RA 3,791,182 A→C Y292S (TAC→TCC)  waaL → O‑antigen ligase
RA 3,798,416 (T)8→7 coding (61/936 nt) rfaS ← LPS core biosynthesis protein RfaS
RA 3,896,621 A→G D147D (GAT→GAC bglB ← 6‑phospho‑beta‑glucosidase BglB
RA 3,898,828 (T)5→4 coding (130/1878 nt) bglF ← PTS beta‑glucoside transporter subunit IIABC
RA 4,073,163 A→T D26V (GAT→GTT)  BW25113_RS20185 → hypothetical protein
RA 4,117,071 T→C F44S (TTC→TCC)  rpmE → 50S ribosomal protein L31
RA 4,153,816 T→C S84P (TCA→CCA)  btuB → TonB‑dependent vitamin B12 receptor BtuB
RA 4,180,586 (A)7→6 intergenic (+333/+77) yjaZ → / ← thiH heat‑shock protein C/2‑iminoacetate synthase ThiH
RA 4,215,724 T→G W657G (TGG→GGG)  metH → methionine synthase
RA 4,306,692 A→T I115N (ATC→AAC)  phnM ← alpha‑D‑ribose 1‑methylphosphonate 5‑triphosphate diphosphatase
RA 4,330,415 A→G intergenic (‑77/+122) adiA ← / ← melR arginine decarboxylase/transcriptional regulator MelR
RA 4,367,563 T→C intergenic (‑24/+65) blc ← / ← ampC lipocalin Blc/BlaEC family class C beta‑lactamase
RA 4,494,539 T→C F222S (TTC→TCC)  nanX → MFS transporter
RA 4,525,805 A→G pseudogene (1198/1241 nt) yjhR → helicase YjhR
RA 4,543,103 A→G E129G (GAA→GGA)  uxuB → fructuronate reductase
RA 4,593,447 T→C S52P (TCC→CCC)  yjjQ → response regulator transcription factor
RA 4,613,222 C→G R238P (CGC→CCC)  lplA ← lipoate‑‑protein ligase LplA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 123103 123142 40 3 [1] [1] 2 aceF pyruvate dehydrogenase complex dihydrolipoyllysine‑residue acetyltransferase
* * ÷ NZ_CP009273 295219 295234 16 2 [1] [1] 5 paoC aldehyde oxidoreductase molybdenum‑binding subunit PaoC
* * ÷ NZ_CP009273 356981 357589 609 2 [0] [1] 2 [lacA] [lacA]
* * ÷ NZ_CP009273 428511 428572 62 2 [1] [1] 2 nrdR transcriptional regulator NrdR
* * ÷ NZ_CP009273 510060 510108 49 2 [1] [1] 2 ybbJ NfeD family protein
* * ÷ NZ_CP009273 757283 757292 10 2 [1] [0] 2 odhB 2‑oxoglutarate dehydrogenase complex dihydrolipoyllysine‑residue succinyltransferase
* * ÷ NZ_CP009273 840317 840324 8 3 [1] [1] 2 ybiO mechanosensitive channel protein
* * ÷ NZ_CP009273 902978 903075 98 2 [1] [1] 2 ybjT SDR family oxidoreductase
* * ÷ NZ_CP009273 1018363 1018437 75 3 [1] [1] 2 yccS YccS family putative transporter
* * ÷ NZ_CP009273 1168405 1168439 35 3 [1] [1] 3 mfd transcription‑repair coupling factor
* * ÷ NZ_CP009273 1183092 1183131 40 2 [1] [0] 2 roxA [50S ribosomal protein L16]‑arginine 3‑hydroxylase
* * ÷ NZ_CP009273 1228611 1228637 27 2 [1] [1] 3 [nhaB] [nhaB]
* * ÷ NZ_CP009273 1240344 1240366 23 4 [1] [1] 4 ymgE GlsB/YeaQ/YmgE family stress response membrane protein
* * ÷ NZ_CP009273 1240707 1240743 37 2 [1] [0] 2 BW25113_RS06225 hypothetical protein
* * ÷ NZ_CP009273 1241375 1241410 36 2 [1] [0] 2 treA alpha,alpha‑trehalase
* * ÷ NZ_CP009273 1242991 1243005 15 5 [1] [0] 2 treA/dhaM alpha,alpha‑trehalase/dihydroxyacetone kinase phosphoryl donor subunit DhaM
* * ÷ NZ_CP009273 1252467 1252511 45 5 [0] [1] 2 ychF redox‑regulated ATPase YchF
* * ÷ NZ_CP009273 1314980 1314993 14 2 [0] [1] 2 trpD bifunctional anthranilate synthase glutamate amidotransferase component TrpG/anthranilate phosphoribosyltransferase TrpD
* * ÷ NZ_CP009273 1317340 1317398 59 2 [1] [0] 2 trpL/yciV trp operon leader peptide/RNase AM
* * ÷ NZ_CP009273 1320582 1320591 10 2 [1] [1] 3 yciQ DUF2207 domain‑containing protein
* * ÷ NZ_CP009273 1357697 1357779 83 2 [1] [1] 2 puuC aldehyde dehydrogenase PuuC
* * ÷ NZ_CP009273 1360134 1360207 74 2 [1] [1] 3 puuE 4‑aminobutyrate transaminase
* * ÷ NZ_CP009273 1410093 1410125 33 2 [1] [1] 2 recE exodeoxyribonuclease VIII
* * ÷ NZ_CP009273 1419592 1419605 14 2 [1] [1] 2 trkG/ynaK Trk system potassium transporter TrkG/ParB/Srx family N‑terminal domain‑containing protein
* * ÷ NZ_CP009273 1433248 1433254 7 2 [1] [1] 2 nifJ pyruvate:ferredoxin (flavodoxin) oxidoreductase
* * ÷ NZ_CP009273 1438434 1438461 28 2 [1] [1] 2 ydbH YdbH family protein
* * ÷ NZ_CP009273 1521152 1521161 10 2 [1] [0] 2 yncG/yncH glutathione S‑transferase family protein/YncH family protein
* * ÷ NZ_CP009273 1524588 1524627 40 2 [0] [1] 3 BW25113_RS26145
BW25113_RS25600
hypothetical protein
protein YncO
* * ÷ NZ_CP009273 1530637 1530649 13 2 [1] [1] 2 narI respiratory nitrate reductase subunit gamma
* * ÷ NZ_CP009273 1532606 1532670 65 2 [1] [1] 2 narH nitrate reductase subunit beta
* * ÷ NZ_CP009273 1533903 1533938 36 2 [1] [1] 2 narZ nitrate reductase Z subunit alpha
* * ÷ NZ_CP009273 1543023 1543095 73 5 [1] [0] 2 fdnG formate dehydrogenase‑N subunit alpha
* * ÷ NZ_CP009273 1591529 1591542 14 2 [1] [1] 2 BW25113_RS07915 ECSE_1600 family autotransporter
* * ÷ NZ_CP009273 1605753 1605794 42 2 [1] [1] 2 dgcF GGDEF domain‑containing protein
* * ÷ NZ_CP009273 1606353 1606375 23 2 [1] [0] 3 yneG DUF4186 domain‑containing protein
* * ÷ NZ_CP009273 1655916 1655942 27 2 [1] [1] 2 ynfF dimethyl sulfoxide reductase subunit A
* * ÷ NZ_CP009273 1663725 1663758 34 2 [1] [1] 2 ynfL LysR family transcriptional regulator
* * ÷ NZ_CP009273 1671117 1671123 7 2 [0] [1] 2 pntA Re/Si‑specific NAD(P)(+) transhydrogenase subunit alpha
* * ÷ NZ_CP009273 1749522 1749534 13 3 [1] [1] 3 fumD/BW25113_RS26160 fumarate hydratase FumD/protein YnhH
* * ÷ NZ_CP009273 1778273 1778291 19 2 [1] [1] 3 fadK medium‑chain fatty‑acid‑‑CoA ligase
* * ÷ NZ_CP009273 1790552 1790568 17 4 [1] [0] 2 pheT phenylalanine‑‑tRNA ligase subunit beta
* * ÷ NZ_CP009273 1790913 1790937 25 2 [1] [1] 2 pheT phenylalanine‑‑tRNA ligase subunit beta
* * ÷ NZ_CP009273 1794646 1794658 13 2 [1] [0] 2 infC translation initiation factor IF‑3
* * ÷ NZ_CP009273 1798288 1798321 34 2 [1] [1] 3 espL1 type III secretion system effector EspL1
* * ÷ NZ_CP009273 1801076 1801109 34 2 [1] [1] 2 pfkB 6‑phosphofructokinase II
* * ÷ NZ_CP009273 1820865 1820933 69 2 [1] [1] 2 astE succinylglutamate desuccinylase
* * ÷ NZ_CP009273 1824482 1824512 31 2 [1] [1] 3 astA arginine N‑succinyltransferase
* * ÷ NZ_CP009273 1851934 1851961 28 2 [1] [1] 2 ydjI ketose‑bisphosphate aldolase
* * ÷ NZ_CP009273 1871561 1871631 71 3 [1] [1] 3 yoaF DUF333 domain‑containing lipoprotein YoaF
* * ÷ NZ_CP009273 1893755 1893812 58 3 [1] [1] 2 pdeD cyclic diguanylate phosphodiesterase
* * ÷ NZ_CP009273 1895721 1895775 55 2 [1] [1] 2 yoaE CNNM family cation transport protein YoaE
* * ÷ NZ_CP009273 1900104 1900199 96 2 [1] [1] 2 mntP manganese efflux pump MntP
* * ÷ NZ_CP009273 1925940 1925973 34 2 [1] [1] 2 purT formate‑dependent phosphoribosylglycinamide formyltransferase
* * ÷ NZ_CP009273 1936342 1936386 45 2 [1] [1] 3 znuA zinc ABC transporter substrate‑binding protein ZnuA
* * ÷ NZ_CP009273 1945211 1945239 29 2 [0] [1] 2 yecD hydrolase
* * ÷ NZ_CP009273 1965359 1965402 44 2 [1] [1] 2 tar methyl‑accepting chemotaxis protein II
* * ÷ NZ_CP009273 1970450 1970450 1 2 [0] [0] 2 motB flagellar motor protein MotB
* * ÷ NZ_CP009273 2001095 2001162 68 2 [1] [1] 2 [amyA]–[yedD] [amyA],[yedD]
* * ÷ NZ_CP009273 2012146 2012224 79 2 [1] [1] 2 fliK flagellar hook‑length control protein FliK
* * ÷ NZ_CP009273 2016831 2016837 7 2 [0] [1] 2 fliR flagellar biosynthetic protein FliR
* * ÷ NZ_CP009273 2029886 2029914 29 2 [1] [0] 2 hchA protein deglycase HchA
* * ÷ NZ_CP009273 2033199 2033252 54 2 [1] [0] 2 msrP protein‑methionine‑sulfoxide reductase catalytic subunit MsrP
* * ÷ NZ_CP009273 2069491 2069493 3 2 [0] [0] 3 BW25113_RS10450 hypothetical protein
* * ÷ NZ_CP009273 2137679 2137694 16 2 [0] [1] 2 dgcE diguanylate cyclase
* * ÷ NZ_CP009273 2243811 2243827 17 3 [0] [1] 2 yeiH YeiH family protein
* * ÷ NZ_CP009273 2303246 2303266 21 2 [0] [1] 2 ada bifunctional DNA‑binding transcriptional regulator/O6‑methylguanine‑DNA methyltransferase Ada
* * ÷ NZ_CP009273 2452508 2452510 3 2 [0] [0] 3 fadJ fatty acid oxidation complex subunit alpha FadJ
* * ÷ NZ_CP009273 2483935 2483968 34 4 [0] [1] 3 oxc oxalyl‑CoA decarboxylase
* * ÷ NZ_CP009273 2499571 2499599 29 2 [1] [1] 2 ypdF aminopeptidase
* * ÷ NZ_CP009273 2564238 2564246 9 2 [1] [1] 2 eutE aldehyde dehydrogenase EutE
* * ÷ NZ_CP009273 2672212 2672259 48 2 [1] [1] 2 yphF substrate‑binding domain‑containing protein
* * ÷ NZ_CP009273 2687954 2687981 28 2 [0] [1] 2 purL phosphoribosylformylglycinamidine synthase
* * ÷ NZ_CP009273 2834010 2834044 35 2 [1] [0] 2 ascF PTS cellobiose/arbutin/salicin transporter subunit IIBC

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 = 32226912 (0.840)3 (0.240) 3/150 1.1 21.0% coding (690/1671 nt) betA choline dehydrogenase
?NZ_CP009273 = 322352 12 (0.960)coding (607/1671 nt) betA choline dehydrogenase
* ? NZ_CP009273 360104 =NA (NA)12 (0.840) 10/170 0.3 42.9% intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 16 (1.120)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 = 377145NA (NA)3 (0.240) 3/152 1.1 NA coding (24/914 nt) BW25113_RS01870 IS3‑like element IS2 family transposase
?NZ_CP009273 = 377199 NA (NA)coding (78/914 nt) BW25113_RS01870 IS3‑like element IS2 family transposase
* ? NZ_CP009273 754093 =14 (0.980)3 (0.230) 3/154 1.1 21.7% intergenic (+232/‑69) sdhB/sucA succinate dehydrogenase iron‑sulfur subunit SdhB/2‑oxoglutarate dehydrogenase E1 component
?NZ_CP009273 754113 = 9 (0.700)intergenic (+252/‑49) sdhB/sucA succinate dehydrogenase iron‑sulfur subunit SdhB/2‑oxoglutarate dehydrogenase E1 component
* ? NZ_CP009273 = 1391494NA (NA)15 (1.070) 12/168 0.2 100% intergenic (+145/‑128) BW25113_RS06980/ynaJ IS5‑like element IS5 family transposase/DUF2534 family protein
?NZ_CP009273 1972747 = 0 (0.000)intergenic (‑293/‑487) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
* ? NZ_CP009273 = 19727511 (0.070)17 (1.190) 16/170 0.1 94.4% intergenic (‑297/‑483) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
?NZ_CP009273 = 2283592 NA (NA)intergenic (‑68/‑387) BW25113_RS11435/narP IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP
* ? NZ_CP009273 = 453250711 (0.770)6 (0.470) 5/152 0.7 44.7% intergenic (+57/‑425) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
?NZ_CP009273 = 4532803 5 (0.390)intergenic (+353/‑129) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA