breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 49,765 C→T intergenic (+134/‑58) kefC → / → folA glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase
RA 49,903 T→G D27E (GAT→GAG folA → type 3 dihydrofolate reductase
RA 1,613,723 G→C G116A (GGC→GCC)  marR → multiple antibiotic resistance transcriptional regulator MarR
MC JC 2,399,465 Δ7 bp coding (650‑656/939 nt) lrhA ← transcriptional regulator LrhA
RA 3,754,231 G→T R127S (CGC→AGC)  selA ← L‑seryl‑tRNA(Sec) selenium transferase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 356969 357580 612 3 [2] [2] 3 [lacA] [lacA]
* * ÷ NZ_CP009273 428519 428545 27 7 [2] [2] 3 nrdR transcriptional regulator NrdR
* * ÷ NZ_CP009273 578969 578997 29 3 [2] [2] 4 BW25113_RS26085/appY tail fiber assembly protein/DNA‑binding transcriptional activator AppY
* * ÷ NZ_CP009273 716401 716486 86 4 [2] [2] 3 BW25113_RS26090/kdpE leader peptide SpeFL/two‑component system response regulator KdpE
* * ÷ NZ_CP009273 950000 950068 69 3 [2] [2] 3 focA/ycaO formate transporter FocA/30S ribosomal protein S12 methylthiotransferase accessory factor YcaO
* * ÷ NZ_CP009273 982677 982728 52 3 [2] [2] 4 ompF/asnS porin OmpF/asparagine‑‑tRNA ligase
* * ÷ NZ_CP009273 1087989 1088023 35 3 [2] [2] 5 pgaA/dgcT poly‑beta‑1,6 N‑acetyl‑D‑glucosamine export porin PgaA/GGDEF domain‑containing protein
* * ÷ NZ_CP009273 1193066 1193096 31 3 [2] [2] 4 BW25113_RS05920 hypothetical protein
* * ÷ NZ_CP009273 1207789 1207803 15 4 [2] [2] 4 BW25113_RS25985/BW25113_RS25535 protein YmgK/hypothetical protein
* * ÷ NZ_CP009273 1329154 1329164 11 3 [1] [0] 8 cysB/ymiA HTH‑type transcriptional regulator CysB/YmiA family putative membrane protein
* * ÷ NZ_CP009273 1459439 1459482 44 3 [2] [2] 3 BW25113_RS25580 hypothetical protein
* * ÷ NZ_CP009273 1576878 1576999 122 3 [2] [2] 4 ydeN/ydeO sulfatase/acid stress response transcriptional regulator YdeO
* * ÷ NZ_CP009273 1888102 1888115 14 3 [2] [2] 3 yoaB/yoaC RidA family protein/DUF1889 family protein
* * ÷ NZ_CP009273 2027327 2027374 48 3 [1] [2] 3 drpB/ompC cell division protein DrpB/porin OmpC
* * ÷ NZ_CP009273 2988612 2988628 17 3 [2] [0] 4 BW25113_RS14850/BW25113_RS26010 response regulator transcription factor/hypothetical protein
* * ÷ NZ_CP009273 3629488 3632094 2607 44 [2] [2] 4 yhiN–[pitA] yhiN,[pitA]
* * ÷ NZ_CP009273 3632126 3632385 260 3 [2] [1] 3 pitA inorganic phosphate transporter PitA
* * ÷ NZ_CP009273 3632423 3632535 113 3 [2] [1] 3 [pitA] [pitA]
* * ÷ NZ_CP009273 3632585 3633264 680 3 [2] [2] 3 uspB uspB
* * ÷ NZ_CP009273 3633323 3633876 554 4 [2] [2] 3 [uspA] [uspA]
* * ÷ NZ_CP009273 3634052 3634686 635 3 [2] [2] 3 [BW25113_RS26230]–[dtpB] [BW25113_RS26230],[dtpB]
* * ÷ NZ_CP009273 3634689 3636237 1549 3 [2] [2] 3 [dtpB]–[rsmJ] [dtpB],[rsmJ]
* * ÷ NZ_CP009273 3636250 3636530 281 3 [1] [2] 3 [rsmJ]–[prlC] [rsmJ],[prlC]
* * ÷ NZ_CP009273 3636584 3637117 534 3 [2] [2] 3 prlC oligopeptidase A
* * ÷ NZ_CP009273 3637119 3638152 1034 3 [2] [2] 3 prlC oligopeptidase A
* * ÷ NZ_CP009273 3638213 3641198 2986 3 [2] [2] 3 [prlC]–dinQ [prlC],rlmJ,gorA,dinQ
* * ÷ NZ_CP009273 3641367 3641560 194 3 [2] [1] 3 dinQ/arsR hypothetical protein/As(III)‑sensing metalloregulatory transcriptional repressor ArsR
* * ÷ NZ_CP009273 3641633 3641804 172 3 [2] [2] 3 dinQ/arsR hypothetical protein/As(III)‑sensing metalloregulatory transcriptional repressor ArsR
* * ÷ NZ_CP009273 3641822 3643413 1592 3 [2] [2] 3 arsR–[arsB] arsR,[arsB]
* * ÷ NZ_CP009273 3643464 3644716 1253 3 [2] [2] 4 [arsB]–[BW25113_RS18155] [arsB],arsC,[BW25113_RS18155]
* * ÷ NZ_CP009273 3644771 3646531–3645398 628–1761 3 [2] [2] 3 [BW25113_RS18155]–BW25113_RS18160 [BW25113_RS18155],BW25113_RS18160

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 360104 =NA (NA)34 (1.230) 23/166 0.2 49.3% intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 35 (1.260)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 1203246 =0 (0.000)16 (0.720) 14/134 0.3 88.9% coding (290/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 1205075 = 4 (0.180)coding (40/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 12032610 (0.000)14 (0.630) 9/134 0.8 87.5% coding (305/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 = 1205058 4 (0.180)coding (57/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 1391494NA (NA)15 (0.550) 8/164 1.2 100% intergenic (+145/‑128) BW25113_RS06980/ynaJ IS5‑like element IS5 family transposase/DUF2534 family protein
?NZ_CP009273 1972747 = 0 (0.000)intergenic (‑293/‑487) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
* ? NZ_CP009273 = 19727512 (0.070)31 (1.120) 17/166 0.4 93.9% intergenic (‑297/‑483) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
?NZ_CP009273 = 2283592 NA (NA)intergenic (‑68/‑387) BW25113_RS11435/narP IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP
* ? NZ_CP009273 2282398 =NA (NA)42 (1.510) 22/166 0.2 95.5% pseudogene (3/2532 nt) yejO autotransporter YejO
?NZ_CP009273 = 3629487 2 (0.070)intergenic (+234/+81) yhiM/yhiN DUF2776 domain‑containing protein/NAD(P)/FAD‑dependent oxidoreductase
* ? NZ_CP009273 4532499 =0 (0.000)14 (0.570) 12/148 0.6 82.3% intergenic (+49/‑433) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
?NZ_CP009273 4532813 = 6 (0.240)intergenic (+363/‑119) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
* ? NZ_CP009273 = 45325070 (0.000)14 (0.570) 10/148 0.8 82.3% intergenic (+57/‑425) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
?NZ_CP009273 = 4532803 6 (0.240)intergenic (+353/‑129) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA