breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 49,765 C→T intergenic (+134/‑58) kefC → / → folA glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase
RA 49,884 C→T P21L (CCG→CTG)  folA → type 3 dihydrofolate reductase
RA 214,743 A→C Y174D (TAC→GAC)  proS ← proline‑‑tRNA ligase
MC JC 2,399,465 Δ7 bp coding (650‑656/939 nt) lrhA ← transcriptional regulator LrhA
RA 3,754,231 G→T R127S (CGC→AGC)  selA ← L‑seryl‑tRNA(Sec) selenium transferase
RA 4,267,454 C→T R20C (CGC→TGC)  soxR → redox‑sensitive transcriptional activator SoxR

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 356976 357574 599 3 [0] [2] 5 [lacA] [lacA]
* * ÷ NZ_CP009273 772577 772678 102 3 [2] [2] 3 tolA cell envelope integrity protein TolA
* * ÷ NZ_CP009273 1635253 1635299 47 3 [2] [1] 3 ynfU/cspB putative zinc‑binding protein YnfU/cold shock‑like protein CspB
* * ÷ NZ_CP009273 2505260 2505318 59 3 [2] [2] 5 mntH Nramp family divalent metal transporter
* * ÷ NZ_CP009273 2897155 2897186 32 3 [2] [2] 3 ygcE/queE FGGY‑family carbohydrate kinase/7‑carboxy‑7‑deazaguanine synthase QueE
* * ÷ NZ_CP009273 3262647 3262692 46 3 [2] [2] 3 yhaC YhaC family protein
* * ÷ NZ_CP009273 3792815 3792825 11 3 [1] [2] 3 waaZ 3‑deoxy‑D‑manno‑oct‑2‑ulosonate III transferase WaaZ

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 360104 =NA (NA)62 (2.480) 33/168 0.0 NA intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 NA (NA)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 1203246 =18 (0.720)4 (0.200) 3/136 2.0 23.1% coding (290/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 1205075 = 12 (0.590)coding (40/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 120326118 (0.720)10 (0.490) 9/136 0.7 42.9% coding (305/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 = 1205058 12 (0.590)coding (57/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 1391494NA (NA)21 (0.850) 13/166 0.6 100% intergenic (+145/‑128) BW25113_RS06980/ynaJ IS5‑like element IS5 family transposase/DUF2534 family protein
?NZ_CP009273 1972747 = 0 (0.000)intergenic (‑293/‑487) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
* ? NZ_CP009273 = 19727511 (0.040)39 (1.560) 26/168 0.1 97.5% intergenic (‑297/‑483) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
?NZ_CP009273 = 2283592 NA (NA)intergenic (‑68/‑387) BW25113_RS11435/narP IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP
* ? NZ_CP009273 = 357534827 (1.080)7 (0.280) 3/168 2.4 20.6% coding (126/1179 nt) hcp Hcp1 family type VI secretion system effector
?NZ_CP009273 3576788 = NA (NA)coding (391/417 nt) BW25113_RS17825 YrhA family protein