breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
RA | 49,765 | C→T | intergenic (+134/‑58) | kefC → / → folA | glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase |
RA | 49,884 | C→T | P21L (CCG→CTG) | folA → | type 3 dihydrofolate reductase |
RA | 214,743 | A→C | Y174D (TAC→GAC) | proS ← | proline‑‑tRNA ligase |
MC JC | 2,399,465 | Δ7 bp | coding (650‑656/939 nt) | lrhA ← | transcriptional regulator LrhA |
RA | 3,754,231 | G→T | R127S (CGC→AGC) | selA ← | L‑seryl‑tRNA(Sec) selenium transferase |
RA | 4,267,454 | C→T | R20C (CGC→TGC) | soxR → | redox‑sensitive transcriptional activator SoxR |
Unassigned missing coverage evidence | ||||||||||
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seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NZ_CP009273 | 356976 | 357574 | 599 | 3 [0] | [2] 5 | [lacA] | [lacA] |
* | * | ÷ | NZ_CP009273 | 772577 | 772678 | 102 | 3 [2] | [2] 3 | tolA | cell envelope integrity protein TolA |
* | * | ÷ | NZ_CP009273 | 1635253 | 1635299 | 47 | 3 [2] | [1] 3 | ynfU/cspB | putative zinc‑binding protein YnfU/cold shock‑like protein CspB |
* | * | ÷ | NZ_CP009273 | 2505260 | 2505318 | 59 | 3 [2] | [2] 5 | mntH | Nramp family divalent metal transporter |
* | * | ÷ | NZ_CP009273 | 2897155 | 2897186 | 32 | 3 [2] | [2] 3 | ygcE/queE | FGGY‑family carbohydrate kinase/7‑carboxy‑7‑deazaguanine synthase QueE |
* | * | ÷ | NZ_CP009273 | 3262647 | 3262692 | 46 | 3 [2] | [2] 3 | yhaC | YhaC family protein |
* | * | ÷ | NZ_CP009273 | 3792815 | 3792825 | 11 | 3 [1] | [2] 3 | waaZ | 3‑deoxy‑D‑manno‑oct‑2‑ulosonate III transferase WaaZ |
Unassigned new junction evidence | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NZ_CP009273 | 360104 = | NA (NA) | 62 (2.480) | 33/168 | 0.0 | NA | intergenic (‑35/+251) | lacZ/BW25113_RS23320 | beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein |
? | NZ_CP009273 | = 360286 | NA (NA) | intergenic (‑217/+69) | lacZ/BW25113_RS23320 | beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein | |||||
* | ? | NZ_CP009273 | 1203246 = | 18 (0.720) | 4 (0.200) | 3/136 | 2.0 | 23.1% | coding (290/630 nt) | BW25113_RS05990 | hypothetical protein |
? | NZ_CP009273 | 1205075 = | 12 (0.590) | coding (40/540 nt) | BW25113_RS06005 | phage tail protein | |||||
* | ? | NZ_CP009273 | = 1203261 | 18 (0.720) | 10 (0.490) | 9/136 | 0.7 | 42.9% | coding (305/630 nt) | BW25113_RS05990 | hypothetical protein |
? | NZ_CP009273 | = 1205058 | 12 (0.590) | coding (57/540 nt) | BW25113_RS06005 | phage tail protein | |||||
* | ? | NZ_CP009273 | = 1391494 | NA (NA) | 21 (0.850) | 13/166 | 0.6 | 100% | intergenic (+145/‑128) | BW25113_RS06980/ynaJ | IS5‑like element IS5 family transposase/DUF2534 family protein |
? | NZ_CP009273 | 1972747 = | 0 (0.000) | intergenic (‑293/‑487) | flhD/uspC | flagellar transcriptional regulator FlhD/universal stress protein UspC | |||||
* | ? | NZ_CP009273 | = 1972751 | 1 (0.040) | 39 (1.560) | 26/168 | 0.1 | 97.5% | intergenic (‑297/‑483) | flhD/uspC | flagellar transcriptional regulator FlhD/universal stress protein UspC |
? | NZ_CP009273 | = 2283592 | NA (NA) | intergenic (‑68/‑387) | BW25113_RS11435/narP | IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP | |||||
* | ? | NZ_CP009273 | = 3575348 | 27 (1.080) | 7 (0.280) | 3/168 | 2.4 | 20.6% | coding (126/1179 nt) | hcp | Hcp1 family type VI secretion system effector |
? | NZ_CP009273 | 3576788 = | NA (NA) | coding (391/417 nt) | BW25113_RS17825 | YrhA family protein |