Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F5 I181 R1	159	32.7	1279082	96.9%	1239430	121.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	480,969	T→C	Q36R (CAG→CGG)	acrA ←	multidrug efflux RND transporter periplasmic adaptor subunit AcrA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	480,969	0	T	C	100.0%	134.2 / NA	37	Q36R (CAG→CGG)	acrA	multidrug efflux RND transporter periplasmic adaptor subunit AcrA
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (16/21); total (16/21)

CAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCTGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTGTTCATATGTAAACCTCGAGTGTCC  >  NZ_CP009273/480845‑481094

cAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCttgt                                                                                                                 >  1:324939/1‑139 (MQ=255)

        tCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTg                                                                                                         >  1:13364/1‑139 (MQ=255)

        tCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTg                                                                                                         >  2:537143/1‑139 (MQ=255)

            gCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTg                                                                                                     <  2:345452/139‑1 (MQ=255)

             cTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTTTTGGGCCTGGTTgt                                                                                                    >  2:33762/1‑139 (MQ=255)

                aCTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTgtc                                                                                                   <  1:472296/137‑1 (MQ=255)

                 cTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTgtcgtc                                                                                                <  1:480497/139‑1 (MQ=255)

                     aGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCCCCTTGTTGGGCCTGTTTTTCGTCACAt                                                                                            >  1:379503/1‑139 (MQ=255)

                           aaCTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGtt                                                                                      <  1:380306/139‑1 (MQ=255)

                                   cGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTa                                                                               <  1:206374/138‑1 (MQ=255)

                                   cGATCCGGTACGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTa                                                                               <  2:533644/138‑1 (MQ=255)

                                    gATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAg                                                                             <  2:501461/139‑1 (MQ=255)

                                    gATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAg                                                                             <  1:484661/139‑1 (MQ=255)

                                    gATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAg                                                                             <  1:150010/139‑1 (MQ=255)

                                        cGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAGCTGc                                                                         >  1:341971/1‑139 (MQ=255)

                                             ggCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAGCTGCCTgag                                                                    <  2:324939/139‑1 (MQ=255)

                                                  tGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAGCTGCCTGAGAGCAt                                                               >  2:416936/1‑139 (MQ=255)

                                                    gTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAGCTGCCTGAGAGCAt                                                               <  1:448471/137‑1 (MQ=255)

                                                         aCCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAGCTGCCTGAGAGCATCAGAACg                                                        <  2:630403/139‑1 (MQ=255)

                                                                aGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAGCTGCCTGAGAGCATCAGAACGACCGCCa                                                 <  1:530479/139‑1 (MQ=255)

                                                                 gCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAGCTGCCTGAGAGCATCAGAACGACCGCCag                                                <  1:416936/139‑1 (MQ=255)

                                                                    cGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAgg                                             >  1:433527/1‑139 (MQ=255)

                                                                                 cAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCCCCTTTTTTGGCCTGTTTGTCGTCACATCCTGTTTGGGCTAAACTGCCTGGGGGCATCAGAACGACCGCCCGAGGCGGAAACCCCCTg                                >  2:613663/1‑139 (MQ=255)

                                                                                 cAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTgtcgt                                                                                                 <  2:12582/74‑1 (MQ=255)

                                                                                 cAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTgtcgt                                                                                                 >  1:12582/1‑74 (MQ=255)

                                                                                 cAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTa                                         <  1:85295/130‑1 (MQ=255)

                                                                                 cAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCct                                   <  2:87298/136‑1 (MQ=255)

                                                                                 cAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTg                                >  1:420328/1‑139 (MQ=255)

                                                                                 cAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCCGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTa                                         >  2:85295/1‑130 (MQ=255)

                                                                                      gTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTTTTTGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAACTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGttttt                           >  2:367900/1‑139 (MQ=255)

                                                                                      gTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTTTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAAACCTCTGttttt                           >  2:159863/1‑139 (MQ=255)

                                                                                      gTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTCGGGCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGttttt                           >  2:309319/1‑139 (MQ=255)

                                                                                          aGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTGtt                        <  2:446010/138‑1 (MQ=255)

                                                                                                              cAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTGTTCATATGTAAACCTCGAGTGTc   <  1:506437/139‑1 (MQ=255)

                                                                                                              cAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTGTTCATATGTAAACCTCGAGTGTc   <  1:309319/139‑1 (MQ=255)

                                                                                                              cAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTGTTCATATGTAAACCTCGAGTGTc   <  2:267557/139‑1 (MQ=255)

                                                                                                                aCGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAGCTGGCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTGTTCATATGTAAAACTCGAGTGTcc  >  1:228889/1‑138 (MQ=255)

CAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCTGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTGTTCATATGTAAACCTCGAGTGTCC  >  NZ_CP009273/480845‑481094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCTGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTGTTCATATGTAAACCTCGAGTGTCCGATTTCAAAT  >  NZ_CP009273/480826‑481104

agatctagtacggtctcgtgggctcggagatgtgtataagagacaGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCC                                                                                                                                  <  SRR3722135.252673/106‑1 (MQ=60)

        AAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTT                                                                                                                          >  SRR3722135.329584/1‑151 (MQ=60)

                CTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGT                                                                                                                  >  SRR3722135.13541/1‑151 (MQ=60)

                             CCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCCCCTTGTTGGGCCTGTTTTTCGTCACATC                                                                                                     >  SRR3722135.384806/1‑151 (MQ=60)

                                  AACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTG                                                                                                  <  SRR3722135.478652/149‑1 (MQ=60)

                                   ACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTA                                                                                               <  SRR3722135.486952/151‑1 (MQ=60)

                                             GAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAGCT                                                                                     <  SRR3722135.385621/151‑1 (MQ=60)

                                                CTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAGCTGCC                                                                                  >  SRR3722135.346822/1‑151 (MQ=60)

                                                     GCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAGCTGCCTGAG                                                                              <  SRR3722135.209266/150‑1 (MQ=60)

                                                      CGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAGCTGCCTGAGAG                                                                            <  SRR3722135.152090/151‑1 (MQ=60)

                                                      CGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAGCTGCCTGAGAG                                                                            <  SRR3722135.491165/151‑1 (MQ=60)

                                                                      GGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAGCTGCCTGAGAGCATCAGAACGACCG                                                              <  SRR3722135.454546/149‑1 (MQ=60)

                                                                            ACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGC                                                      >  SRR3722135.439440/1‑151 (MQ=60)

                                                                                  AAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAAC                                                <  SRR3722135.537495/151‑1 (MQ=60)

                                                                                   AGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACC                                               <  SRR3722135.422664/151‑1 (MQ=60)

                                                                                         GTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTctgtctcttatacacatctccgagcccccgagaccgtactagatttcgtatgccg                                         >  SRR3722135.12749/1‑96 (MQ=60)

                                                                                         GTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAGCTGCCTGAGAGCATCAGAACGACCGCCCGAGGCGTAAACCCTCTGT                                         >  SRR3722135.426090/1‑151 (MQ=60)

                                                                                           TGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTT                                        <  SRR3722135.86435/150‑1 (MQ=60)

                                                                                                                   GACTGTTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACAATCTGTTAGGGGTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAAACCTCTGTTTTTGTTCATATGTAAACATCGAGTG               >  SRR3722135.206263/1‑151 (MQ=60)

                                                                                                                        TTACTACGCCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAGCTGGCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTGTTCATATGTAAAACTCGAGTGTCCG           >  SRR3722135.232094/1‑150 (MQ=60)

                                                                                                                                CCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTGTTCATATGTAAACCTCGAGTGTCCGATTTCAAAT  <  SRR3722135.313747/151‑1 (MQ=60)

                                                                                                                                CCAACGGCGGGCATCCGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTGTTCATATGTAAACCTCGAGTGTCCGATTTCAAAT  <  SRR3722135.513221/151‑1 (MQ=60)

CTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCTGCTGGCCACCTTGTTGGGCCTGTTTGTCGTCACATCCTGTTAGGGCTAAGCTGCCTGAGAGCATCAGAACGACCGCCAGAGGCGTAAACCCTCTGTTTTTGTTCATATGTAAACCTCGAGTGTCCGATTTCAAAT  >  NZ_CP009273/480826‑481104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`