Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F4 I96 R1
|
227 |
23.3 |
882352 |
98.0% |
864704 |
126.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
861,316 |
T→C |
I169V (ATT→GTT) |
moeA ← |
molybdopterin molybdotransferase MoeA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 861,316 | 0 | T | C | 100.0%
| 64.8
/ NA
| 19 | I169V (ATT→GTT) | moeA | molybdopterin molybdotransferase MoeA |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (7/12); total (7/12) |
CTAACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAATCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGCAGAGATATCTTCACCGCGACGGCGAATATTTTGCCCGCTACGCACTTCAGCAGTAAAACGCA > NZ_CP009273/861181‑861450
|
cTAACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAAcccc > 2:346391/1‑139 (MQ=255)
aGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAg < 2:276013/139‑1 (MQ=255)
tACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAAt > 2:390210/1‑138 (MQ=255)
agaCGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTgg > 1:390682/1‑137 (MQ=255)
gTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCg < 1:346391/138‑1 (MQ=255)
tCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTa < 1:227823/138‑1 (MQ=255)
aCCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGATTTCCCGCCGGGAAaacaac < 1:425324/138‑1 (MQ=255)
gggCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCa < 2:358434/139‑1 (MQ=255)
cTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGCa < 1:105452/139‑1 (MQ=255)
gAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGCa > 2:431711/1‑136 (MQ=255)
tcatcaCCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCAc > 1:324942/1‑127 (MQ=255)
tcatcaCCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCAc < 2:324942/127‑1 (MQ=255)
tcaCCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGCAGAGATATCtt > 1:145603/1‑139 (MQ=255)
cGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAg < 1:28898/63‑1 (MQ=255)
cGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAg > 2:28898/1‑63 (MQ=255)
ttACGAATCACCGGAACTTCGGCAACCCCCAGTGAAg > 2:58072/1‑37 (MQ=255)
ttACGAATCACCGGAACTTCGGCAACCCCCAGTGAAg < 1:58072/37‑1 (MQ=255)
aCGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGCAGAGATATCTTCACCGCGACGGCGAATATTTTGCCCGCTACg < 2:390682/138‑1 (MQ=255)
aCGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGCAGAGATATCTTCACCGCGACGGCGAATATTTTGCCCGCTACg < 1:431711/138‑1 (MQ=255)
gCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGCAGAGATATCTTCACCGCGACGGCGAATATTTTGCCCGCTACGCACTTCAGCAGTAAAacgca < 2:145603/139‑1 (MQ=255)
|
CTAACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAATCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGCAGAGATATCTTCACCGCGACGGCGAATATTTTGCCCGCTACGCACTTCAGCAGTAAAACGCA > NZ_CP009273/861181‑861450
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAATCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGCAGAGATATCTTCACCGCGACGGCGAATATTTTGCCCGCTACGCACTTCAGCAGTAAAACGCACGCCA > NZ_CP009273/861171‑861455
|
CCCAACTGCTCTAACATCATGTGTACGGCGGGACGGTTGGTATCGTAGATTTGGCCGTCCCCCAGCGGCTGGCCGGGCAACTGGAGTTCATCACCGCTAAAAAAAAGCGCTACACGCACCTTACGAATCACCGGAACTTCAGCAATCCCC < SRR3722119.192263/150‑1 (MQ=60)
caagcagaagacggcatacgagattcgccttagtctcgtgggctcggagatgtgtataagagacaGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGC < SRR3722119.29157/86‑1 (MQ=60)
gagaagtaatgcgttttttttttttcaagcagaagacggcatacgagattcgccttagtctcgtgggctcggagatgtgtataagagacaGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGC < SRR3722119.58610/60‑1 (MQ=60)
GGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGC > SRR3722119.394594/1‑149 (MQ=60)
GGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCG < SRR3722119.349909/150‑1 (MQ=60)
ATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGT < SRR3722119.230232/150‑1 (MQ=60)
GACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGATTTCCCGCCGGGAAAACAACCGCACCTGCAG < SRR3722119.429582/150‑1 (MQ=60)
GCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGCAGAGATAct > SRR3722119.328258/1‑149 (MQ=60)
ACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGCAGAGATATCTTC < SRR3722119.106489/151‑1 (MQ=60)
ACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGCAGAGATATCTTC > SRR3722119.147101/1‑151 (MQ=60)
TACGAATCACCGGAACTTCGGCAGCCCCCAGTGAAGCAATTACTGGCGGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGCAGAGATATCTTCACCGCGACGGCGAATATTTTGCCCGCTACGCACTTCAGCAG < SRR3722119.436032/150‑1 (MQ=60)
AACTTCGGCAACCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGCAGAGATATCTTCACCGCGACGGCGAATATTTTGCCCGCTACGCACTTCAGCAGctgtctcttatacc > SRR3722119.161862/1‑137 (MQ=60)
|
CCCAACTGTTCTAACATCAGGTGTACGGCGAGACGGTTGGTATCGTAGATTTGGCCGTCGCCCAGCGGCTGACCGGGCAACTGGAGTTCATCACCGGTAGAAAAAAGCGCTACACGCACTTTACGAATCACCGGAACTTCGGCAATCCCCAGTGAAGCAATTACTGGCAGCTCTGCGGTAGTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGCAGAGATATCTTCACCGCGACGGCGAATATTTTGCCCGCTACGCACTTCAGCAGTAAAACGCACGCCA > NZ_CP009273/861171‑861455
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
