Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F5 I178 R1
|
167 |
28.0 |
1137820 |
95.3% |
1084342 |
115.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
859,589 |
A→G |
A164A (GCA→GCG) |
fsa → |
fructose‑6‑phosphate aldolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 859,589 | 0 | A | G | 100.0%
| 85.1
/ NA
| 24 | A164A (GCA→GCG) | fsa | fructose‑6‑phosphate aldolase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (11/13); total (11/13) |
TGGCAGGTGCGGAATATGTTGCGCCTTACGTTAATCGTATTGATGCTCAGGGCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCAGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGC > NZ_CP009273/859462‑859722
|
tGGCAGGTGCGGAATATGTTGCGCCTTACGTAAATCGTATTGATGCTCAGGGCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCa > 1:257220/1‑138 (MQ=255)
ggTGCGGAATATGTTGCGCCTTACGTTAATCGTATTGATGCTCAGGGCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAAccc < 2:129191/139‑1 (MQ=255)
tGCGGAATATGTTGCGCCTTACGTTAATCGTATTGATGCTCAGGGCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGgcggcg < 2:440730/124‑1 (MQ=255)
tGCGGAATATGTTGCGCCTTACGTTAATCGTATTGATGCTCAGGGCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGgcggcg > 1:440730/1‑124 (MQ=255)
gAATATGTTGCGCCTTACGTTAATCGTATTGATGCTCAGGGCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTc < 1:535473/139‑1 (MQ=255)
tACGTTAATCGTATTGATGCTCAGGGCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCt > 2:27858/1‑139 (MQ=255)
gTATTGATGCTCAGGGCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAgg < 2:210782/139‑1 (MQ=255)
ttGATGCTCAGGGCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGAtg > 1:482199/1‑139 (MQ=255)
tGCTCAGGGCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGaa > 1:420340/1‑139 (MQ=255)
gCTCAGGGCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTCAGgcg < 2:411850/111‑1 (MQ=255)
gCTCAGGGCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTCAGgcg > 1:411850/1‑111 (MQ=255)
gCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTc > 1:188990/1‑90 (MQ=255)
gCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTc < 2:188990/90‑1 (MQ=255)
aCTGTGACCGACTTACCCCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTgg < 2:223129/139‑1 (MQ=255)
tgACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGcac < 2:482199/138‑1 (MQ=255)
aCTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGAc < 1:359048/83‑1 (MQ=255)
aCTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGAc > 2:359048/1‑83 (MQ=255)
atgcatgcGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGa < 2:283043/83‑1 (MQ=255)
atgcatgcGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGa > 1:283043/1‑83 (MQ=255)
tgcatgcGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTCAGgcg > 1:292867/1‑56 (MQ=255)
tgcatgcGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTCAGgcg < 2:292867/56‑1 (MQ=255)
tgcatgcGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGa < 1:27858/139‑1 (MQ=255)
gcAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCtgtg > 2:189863/1‑139 (MQ=255)
cAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTgg > 1:254680/1‑139 (MQ=255)
ggCGAAATTGCTGGCGGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCg < 2:452710/139‑1 (MQ=255)
cTGGCGGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGc < 2:254680/139‑1 (MQ=255)
|
TGGCAGGTGCGGAATATGTTGCGCCTTACGTTAATCGTATTGATGCTCAGGGCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCAGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGC > NZ_CP009273/859462‑859722
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GCTGTCGGCGCTGGCAGGTGCGGAATATGTTGCGCCTTACGTTAATCGTATTGATGCTCAGGGCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCAGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGC > NZ_CP009273/859451‑859711
|
GCTGTCGGCGCTGGCAGGTGCGGAATATGTTGCGCCTTACGTAAATCGTATTGATGCTCAGGGCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAA > SRR3722132.262111/1‑150 (MQ=60)
GCGCTGGCAGGTGCGGAATATGTTGCGCCTTACGTTAATCGTATTGATGCTCAGGGCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCtgtc > SRR3722132.448664/1‑147 (MQ=60)
GGAATATGTTGCGCCTTACGTTAATCGTATTGATGCTCAGGGCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGAC < SRR3722132.544933/151‑1 (MQ=60)
agatctagtacggtctcgtgggctcggagatgtgtataagagacagGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGC < SRR3722132.365657/105‑1 (MQ=60)
CGTTAATCGTATTGATGCTCAGGGCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGT > SRR3722132.490806/1‑151 (MQ=60)
AATCGTATTGATGCTCAGGGCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAAT > SRR3722132.427937/1‑151 (MQ=60)
ATCGTATTGATGCTCAGGGCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTctgtctcttatacacatc > SRR3722132.419330/1‑133 (MQ=60)
GGGCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACctgtctcttatacacatctccgagcccacgagaccgtac > SRR3722132.192545/1‑112 (MQ=60)
AGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCctgtctcttatacacatctccgagcccacgagaccgtactagatct > SRR3722132.288408/1‑105 (MQ=60)
GTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTctgtctcttatacacatctccgagcccacgagaccgtactagatctcgtatgccgtcttctgcttgaaaaaa > SRR3722132.298430/1‑78 (MQ=60)
ATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGG < SRR3722132.28342/151‑1 (MQ=60)
TGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGC > SRR3722132.259524/1‑151 (MQ=60)
|
GCTGTCGGCGCTGGCAGGTGCGGAATATGTTGCGCCTTACGTTAATCGTATTGATGCTCAGGGCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCAGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGC > NZ_CP009273/859451‑859711
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
