Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
343 |
268.7 |
10873516 |
97.0% |
10547310 |
150.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
CP009974 |
1,897,027 |
4 bp→GACC |
intergenic (‑4805/‑3789) |
RPPX_08470 ← / → RPPX_08480 |
tryptophan synthase subunit beta/hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP009974 | 1,897,027 | 0 | C | G | 100.0%
| 19.2
/ NA
| 19 | intergenic (‑4805/‑3792) | RPPX_08470/RPPX_08480 | tryptophan synthase subunit beta/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (9/10); total (9/10) |
| * | CP009974 | 1,897,028 | 0 | G | A | 100.0%
| 18.9
/ NA
| 19 | intergenic (‑4806/‑3791) | RPPX_08470/RPPX_08480 | tryptophan synthase subunit beta/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (9/10); total (9/10) |
| * | CP009974 | 1,897,029 | 0 | T | C | 100.0%
| 19.3
/ NA
| 19 | intergenic (‑4807/‑3790) | RPPX_08470/RPPX_08480 | tryptophan synthase subunit beta/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (9/10); total (9/10) |
| * | CP009974 | 1,897,030 | 0 | G | C | 100.0%
| 19.3
/ NA
| 19 | intergenic (‑4808/‑3789) | RPPX_08470/RPPX_08480 | tryptophan synthase subunit beta/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (9/10); total (9/10) |
GTCCAGCGCCTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATCGTGGTCTGA‑GCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAACTTGCCAACGTCC > CP009974/1896922‑1897081
||||
gTCCAGCGCCTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTCGAG‑GCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTg < 2:3755251/151‑1 (MQ=11)
tCCAGCGCCTGGCTCACTTTCAGAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTCGAG‑GCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGc > 2:4830471/1‑151 (MQ=11)
ccAGCGCCTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTCGAG‑GCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGcc < 1:4248043/151‑1 (MQ=11)
ccAGCGCCTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTC‑GAGGCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGcc < 2:2527334/151‑1 (MQ=11)
cAGCGCCTGGCTCACTTTCCCAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTC‑GAGGCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGccg < 2:3620689/151‑2 (MQ=11)
cAGCGCCTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTC‑GAGGCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGccg < 1:4890205/151‑2 (MQ=11)
cAGCGCCTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTC‑GAGGCCGGCGTCGGGTTCGGGGCCAGTTGCTCGAAGTTGccg > 2:3959584/1‑150 (MQ=11)
aGCGCCTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTCGAG‑GCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGccgc > 1:3264377/1‑149 (MQ=11)
aGCGCCTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTC‑GAGGCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGccgc < 1:966047/151‑3 (MQ=11)
aGCGCCTGGCTCACTTGCACAGGAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTCGAG‑GCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGccgc < 2:4730881/151‑3 (MQ=11)
gcgcCTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTCGAG‑GCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGccgcc > 2:1274501/1‑148 (MQ=1)
cgcCTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTC‑GAGGCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGccgccc < 2:2627404/151‑5 (MQ=11)
gcCTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTCGAG‑GCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGccgcccg < 2:3264225/151‑6 (MQ=11)
gcCTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTCGAG‑GCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGccgcccg > 1:4133062/1‑146 (MQ=1)
gcCTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTC‑GAGGCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGccgcccg > 2:1769098/1‑146 (MQ=1)
gcCGGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTC‑GAGGCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGccgcccg < 2:4791902/151‑6 (MQ=11)
ccTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTCGAG‑GCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGccgcccgt > 1:4935977/1‑145 (MQ=11)
ccTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTC‑GAGGCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGccgcccgt > 1:4935996/1‑145 (MQ=1)
cTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTCGAG‑GCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGccgcccgtg > 2:3908472/1‑144 (MQ=1)
||||
GTCCAGCGCCTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATCGTGGTCTGA‑GCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAACTTGCCAACGTCC > CP009974/1896922‑1897081
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
