Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
336 |
367.5 |
14491858 |
96.8% |
14028118 |
150.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
CP009974 |
1,897,027 |
4 bp→GACC |
intergenic (‑4805/‑3789) |
RPPX_08470 ← / → RPPX_08480 |
tryptophan synthase subunit beta/hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP009974 | 1,897,027 | 0 | C | G | 100.0%
| 12.6
/ NA
| 12 | intergenic (‑4805/‑3792) | RPPX_08470/RPPX_08480 | tryptophan synthase subunit beta/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (9/3); total (9/3) |
| * | CP009974 | 1,897,028 | 0 | G | A | 100.0%
| 12.3
/ NA
| 12 | intergenic (‑4806/‑3791) | RPPX_08470/RPPX_08480 | tryptophan synthase subunit beta/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (9/3); total (9/3) |
| * | CP009974 | 1,897,029 | 0 | T | C | 100.0%
| 12.6
/ NA
| 12 | intergenic (‑4807/‑3790) | RPPX_08470/RPPX_08480 | tryptophan synthase subunit beta/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (9/3); total (9/3) |
| * | CP009974 | 1,897,030 | 0 | G | C | 100.0%
| 12.6
/ NA
| 12 | intergenic (‑4808/‑3789) | RPPX_08470/RPPX_08480 | tryptophan synthase subunit beta/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (9/3); total (9/3) |
TCCAGCGCCTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATCGTGGTCTGA‑GCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAACTTGCCAACGTCC > CP009974/1896923‑1897081
||||
tCCAGCGCCTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTCGAG‑GCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGc > 1:5289578/1‑151 (MQ=11)
ccAGCGCCTGGCTCACTTTCACGGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTC‑GAGGCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGcc > 1:4154857/1‑151 (MQ=11)
cAGCGCCTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTCGAG‑GCCGGCGTCGGGTTCGGGGTCCGTTGCTCGAAGTTGccg > 2:6493642/1‑150 (MQ=11)
aGCGCCTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTCGAG‑GCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGccgc > 2:4818064/1‑149 (MQ=11)
aGCGCCTGGCTCACCTTCACAGTAAAGGAAGGTTGCTGGTCTGCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTC‑GAGGCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGccgc < 2:6805679/151‑3 (MQ=11)
cgcCTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTC‑GAGGCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGcggccc > 1:2921107/1‑146 (MQ=11)
cgcCTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTC‑GAGGCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGccgccc > 2:3696975/1‑147 (MQ=1)
gcCTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTC‑GAGGCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGccgcccg < 1:3520682/151‑6 (MQ=11)
ccTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTCGAG‑GCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGccgcccgt > 1:259545/1‑145 (MQ=11)
ccTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTC‑GAGGCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGccgcccgt < 1:6436964/151‑7 (MQ=11)
ccTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTC‑GAGGCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGccgcccgt > 1:6843030/1‑145 (MQ=11)
cTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATGACCGTCGAG‑GCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAAGTTGccgcccgtg > 2:6072922/1‑144 (MQ=11)
||||
TCCAGCGCCTGGCTCACTTTCACAGTAAAGGAAGGTTGCTGGTCTTCGGTCACATTGCCATTGCTGACAATGCTTACGGTGACGGCATCGATGCTGTCATTGATCGTGGTCTGA‑GCCGGCGTCGGGTTCGGGGTCAGTTGCTCGAACTTGCCAACGTCC > CP009974/1896923‑1897081
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
