Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I1 R1
|
368 |
332.2 |
13092066 |
97.0% |
12699304 |
150.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
CP009974 |
1,897,927 |
A→G |
intergenic (‑5705/‑2892) |
RPPX_08470 ← / → RPPX_08480 |
tryptophan synthase subunit beta/hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP009974 | 1,897,927 | 0 | A | G | 100.0%
| 47.3
/ NA
| 29 | intergenic (‑5705/‑2892) | RPPX_08470/RPPX_08480 | tryptophan synthase subunit beta/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (14/15); total (14/15) |
GCACAGGGCGATGACGTCTACCAGGACGCAGGCCAGATCAGTCTGGGCATCACGTCCGCTGTGGATATAGACGGTCACACCTTTGAGAACCTGGAACTGGGTGGTGCTGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTAGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGGGTGGCA > CP009974/1897784‑1897972
|
gcgcaaggCGATGACGTCTACCAGGACGCTGGCCAGATCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCATCGACGAAGTGGTGGCCa < 1:3208293/145‑1 (MQ=1)
caGGGCGATGACGTCTACCAGGACGCAGGCCAGATCAGTCTGGGCATCACGTCCGCTGTGGATATAGACGGTCACACCTTTGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTCCAAGTAACCGACACCACCGACGAAGTGGTGGCCAAGc < 2:4314218/151‑1 (MQ=255)
aGGGCGATGACGTCTACCAGGACGCAGGCCAGATCAGTCTGGGCATCACGTCCGCTGTGGATATAGACGGTCACACCTTTGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTCCAAGTAACCGACACCACCGACGAAGTGGTGGCCAAGCt > 1:2396124/1‑151 (MQ=255)
aTGACGTCTACCAGGACGCAGGCCAGAGCAGTCTGGGCATCACGTCCGCTGTGGATATAGACGGTCACACCTTTGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTCCAAGTAACCGACACCACCGACGAAGTGGTGGCCAAGCTGACcgc < 2:3720828/151‑1 (MQ=11)
gACGTCTACCAGGACGCAGGCCAGATCAGTCTGGGCATCACGTCCGCTGTGGATATAGACGGTCACACCTTTGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTCCAAGTAACCGACACCACCGACGAAGTGGTGGCCAAGCTGACcgcca > 1:510117/1‑149 (MQ=11)
gACGTCTACCAGGACGCAGGCCAGATCAGTCTGGGCATCACGTCCGCTGTGGATATAGACGGTCACACCTTTGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTCCAAGTAACCGACACCACCGACGAAGTGGTGGCCAAGCTGACcgcca < 1:676121/151‑3 (MQ=255)
tCTACCAGGACGCAGGCCAGATCAGTCTGGGCATCACGTCCGCTGTGGATATAGACGGTCACACCTTTGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTCCAAGTAACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCCAcccc < 1:895027/151‑1 (MQ=11)
cTACCAGGACGCAGGCCAGATCAGTCTGGGCATCACGTCCGCTGTGGATATAGACGGTCACACCTTTGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTCCAAGTAACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCCAcccct < 2:5396886/151‑2 (MQ=11)
cTACCAGGACGCAGGCCAGATCAGTCTGGGCACCACGTCCGCTGTGGATATAGACGGTCACACCTTTGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTCCAAGTAACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCCAcccct < 2:5396887/151‑2 (MQ=11)
ggACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGCGCTGCTTCGGTTCAAGTGACCGACACCCGCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTc > 2:2230284/1‑151 (MQ=1)
gctggCCAGATCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCg > 2:5052860/4‑151 (MQ=11)
tggCCAGATCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGaa > 1:2437004/2‑151 (MQ=11)
tggCCAGATCAGTCTGGGCATCACGTCCGCTGCGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGaa > 2:683975/2‑151 (MQ=11)
ggCCAGATCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAg < 1:291685/151‑1 (MQ=11)
ggCCAGATCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAg > 2:6340495/1‑151 (MQ=11)
ggCCAGATCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAg > 2:6340453/1‑151 (MQ=11)
ggCCAGATCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAg < 1:289640/151‑1 (MQ=11)
ggCCAGATCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAg > 1:2255712/1‑151 (MQ=11)
ccAGATCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAggc < 2:2779527/151‑2 (MQ=11)
ccAGATCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAggc < 1:1507242/151‑2 (MQ=11)
cAGATCAGGCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAggcg > 1:1555248/1‑149 (MQ=11)
aGATCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAggcgg > 2:1581795/1‑148 (MQ=11)
gATGAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAggcggt < 2:1443302/151‑5 (MQ=11)
aTCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAggcggtg > 2:456116/1‑146 (MQ=11)
aTCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAggcggtg < 2:2313163/151‑6 (MQ=11)
tCAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCCACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAggcggtga < 2:4018000/151‑7 (MQ=11)
cAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAggcggtgag > 2:1091716/1‑144 (MQ=11)
cAGTCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAggcggtgag < 2:730304/151‑8 (MQ=11)
aGACGGTCACACCTTCGAGAACCTGGAACTGGGCGGTGCTGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGaa > 1:1345821/1‑112 (MQ=11)
|
GCACAGGGCGATGACGTCTACCAGGACGCAGGCCAGATCAGTCTGGGCATCACGTCCGCTGTGGATATAGACGGTCACACCTTTGAGAACCTGGAACTGGGTGGTGCTGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTAGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGGGTGGCA > CP009974/1897784‑1897972
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
