Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I1 R1 404 265.1 10478538 96.8% 10143224 150.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 5,007,281 A→T Y68F (TAC→TTC)  RPPX_22185 → phenylacetate‑‑CoA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099745,007,2810AT99.6% 822.8 / NA 227Y68F (TAC→TTC) RPPX_22185phenylacetate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (124/101);  minor base C (1/0);  total (125/102)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 228 total aligned reads displayed.

AGCGTCGATGCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTACCCCTTCACCGGCAAGAACGATCTGCGCGACAACTACCCCTTCGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGCGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACAC  >  CP009974/5007133‑5007428
                                                                                                                                                    |                                                                                                                                                   
aGCGTCGATGCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTGTGCCGAGTACGGCACCCACCCTGATGACGTCAAGTGCCTGGAAGACCTGGCGAAGTccc                                                                                                                                                   >  2:3197128/1‑151 (MQ=255)
 gCGTCGATGCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTccc                                                                                                                                                  >  1:4242299/1‑151 (MQ=255)
  cGTCGATGCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAATTGCCTGGAAGACCTGGCGAATTTcccc                                                                                                                                                 >  2:1272652/1‑151 (MQ=255)
  cGTCGATGCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTcccc                                                                                                                                                 <  1:66234/151‑1 (MQ=255)
   gTCGATGCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCt                                                                                                                                                >  2:2316049/1‑151 (MQ=255)
    tCGATGCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCtt                                                                                                                                               >  1:3076996/1‑151 (MQ=255)
     cGATGCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTc                                                                                                                                              >  2:4155299/1‑151 (MQ=255)
      gATGCCCTGCGCCAGCACCAGCTGGAGCGACTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCACGTGCCTGGAAGACCTGGCGAAGTTCCCCTTcc                                                                                                                                             >  2:3077213/1‑150 (MQ=255)
        tGCCCTGCGCCAGCGCCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCAcc                                                                                                                                           <  2:3996282/151‑1 (MQ=255)
         gCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCg                                                                                                                                          <  1:4121409/151‑1 (MQ=255)
          cccTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCgg                                                                                                                                         <  1:4155299/151‑1 (MQ=255)
                 ccAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAAc                                                                                                                                  <  1:4191789/151‑1 (MQ=255)
                 ccAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTCACGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCCGCacgcac                                                                                                                                  >  2:2974103/1‑146 (MQ=255)
                     cACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCaagaaagacc                                                                                                                              <  2:4297836/151‑3 (MQ=255)
                      aCCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCt                                                                                                                             <  2:2438856/151‑1 (MQ=255)
                       ccAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTg                                                                                                                            <  1:950482/151‑1 (MQ=255)
                       ccAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTg                                                                                                                            >  1:362282/1‑151 (MQ=255)
                        cAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTgc                                                                                                                           >  1:4435823/1‑151 (MQ=255)
                        cAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTgc                                                                                                                           <  1:3693598/151‑1 (MQ=255)
                         aGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACTACCTgcg                                                                                                                          >  2:354795/1‑151 (MQ=255)
                         aGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAACAACGACCTgcc                                                                                                                          >  2:3913749/1‑150 (MQ=255)
                            tGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGa                                                                                                                       >  1:3103784/1‑151 (MQ=255)
                             ggAGCGTCTGCGCTGGAGCCTGCAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCCCCCTGATGACCTCAAGTGCCTGGAAGGCCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGAc                                                                                                                      <  2:4624295/151‑1 (MQ=255)
                             ggAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAAGGACCTGCGCGAc                                                                                                                      >  1:1472453/1‑151 (MQ=255)
                              gAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACa                                                                                                                     <  1:76973/151‑1 (MQ=255)
                                 cGTCTGCGCTGGAGCCTGAATGACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACt                                                                                                                  <  2:5136939/151‑1 (MQ=255)
                                  gTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGGCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACtg                                                                                                                 >  1:2284594/1‑150 (MQ=255)
                                    cTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGCCCTGCGCGACAACTAcc                                                                                                               <  2:67745/151‑1 (MQ=255)
                                    cTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTAcc                                                                                                               <  2:288034/151‑1 (MQ=255)
                                      gcgcTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTAcccc                                                                                                             <  2:781858/151‑1 (MQ=255)
                                      gcgcTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTAcccc                                                                                                             >  1:2074195/1‑151 (MQ=255)
                                      gcgcTGGAGCCCGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTAcccc                                                                                                             <  1:4803373/151‑1 (MQ=255)
                                        gcTGGAGGCTGATGCACGCCTGCGACAATGTGCCGTTGTCCCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCACGTGCCTGGAAGACCTGGCGCAGTTCCCCTTCACCGGCAAGAACGGCCTGCGCGACAACTACCCCta                                                                                                           <  2:912873/151‑2 (MQ=255)
                                        gcTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCtc                                                                                                           >  1:2648509/1‑150 (MQ=255)
                                        gcTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCta                                                                                                           >  2:2077201/1‑150 (MQ=255)
                                           ggAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACgg                                                                                                        <  2:2564873/151‑1 (MQ=255)
                                              gCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCAt                                                                                                     <  2:1348324/151‑1 (MQ=255)
                                                 tGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGtt                                                                                                  <  2:3986994/151‑1 (MQ=255)
                                                 tGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGtt                                                                                                  <  1:2590825/151‑1 (MQ=255)
                                                  gAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGttt                                                                                                 >  1:2352976/1‑151 (MQ=255)
                                                  gAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGctt                                                                                                 >  1:2824771/1‑151 (MQ=255)
                                                         gCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGc                                                                                          <  2:4736614/151‑1 (MQ=255)
                                                         gCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGc                                                                                          <  2:2087054/151‑1 (MQ=255)
                                                         gCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGc                                                                                          <  1:3237149/151‑1 (MQ=255)
                                                           cTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGccc                                                                                        >  2:4249320/1‑151 (MQ=255)
                                                           cTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGccc                                                                                        >  2:2374134/1‑151 (MQ=255)
                                                             aCGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCTCCa                                                                                      >  1:183587/1‑151 (MQ=255)
                                                                aCAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGga                                                                                   >  2:4131009/1‑151 (MQ=255)
                                                                 cAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGgaa                                                                                  >  1:3891480/1‑151 (MQ=255)
                                                                      tgCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAgt                                                                             <  2:2475296/151‑1 (MQ=255)
                                                                         cGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAgtggt                                                                          <  2:3688599/151‑1 (MQ=255)
                                                                          gTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAgtggtg                                                                         <  2:1470107/151‑1 (MQ=255)
                                                                          gTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAgtggtg                                                                         <  1:3005275/151‑1 (MQ=255)
                                                                           ttGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTgc                                                                        >  1:1024025/1‑151 (MQ=255)
                                                                            tGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTgcg                                                                       >  1:1485800/1‑151 (MQ=255)
                                                                             gTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTgcgc                                                                      >  2:4210186/1‑151 (MQ=255)
                                                                              tACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGcc                                                                     >  1:3421221/1‑151 (MQ=255)
                                                                               aCCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCt                                                                    <  2:1574027/151‑1 (MQ=255)
                                                                               aCCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGCGGTGCGCCt                                                                    >  2:4344994/1‑151 (MQ=255)
                                                                                 cGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGc                                                                  >  2:239897/1‑151 (MQ=255)
                                                                                 cGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGc                                                                  >  1:1753328/1‑151 (MQ=255)
                                                                                 cGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGc                                                                  >  1:4918031/1‑151 (MQ=255)
                                                                                   ccAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCAt                                                                >  1:1803786/1‑151 (MQ=255)
                                                                                       cgcTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCt                                                            <  2:172918/151‑1 (MQ=255)
                                                                                        gcTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTc                                                           <  2:3738217/151‑1 (MQ=255)
                                                                                        gcTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTc                                                           <  1:2736394/151‑1 (MQ=255)
                                                                                         cTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTcc                                                          >  1:2118520/1‑151 (MQ=255)
                                                                                          tttGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCa                                                         <  2:3893504/151‑1 (MQ=255)
                                                                                             gCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAgtg                                                      <  2:3404794/151‑3 (MQ=255)
                                                                                                  gTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGcacc                                                 >  1:2679093/1‑151 (MQ=255)
                                                                                                   tACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGcacca                                                <  2:1239277/151‑1 (MQ=255)
                                                                                                      ggCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCg                                             >  2:1390841/1‑151 (MQ=255)
                                                                                                       gCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCgg                                            <  1:1294783/151‑1 (MQ=255)
                                                                                                                cTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGAc                                   <  2:1977440/151‑1 (MQ=255)
                                                                                                                 tgatgaCCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACg                                  <  1:3121378/151‑1 (MQ=255)
                                                                                                                 tgatgaCCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACg                                  <  2:4368758/151‑1 (MQ=255)
                                                                                                                  gatgaCCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATg                                                               >  2:2716232/1‑121 (MQ=255)
                                                                                                                    tgaCCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACggtg                               <  2:166993/151‑1 (MQ=255)
                                                                                                                      aCCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACggtggc                             >  2:618904/1‑150 (MQ=255)
                                                                                                                       ccTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTc                            >  2:2141790/1‑151 (MQ=255)
                                                                                                                          cAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGc                         >  2:3713488/1‑151 (MQ=255)
                                                                                                                          cAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGc                         >  1:1667531/1‑151 (MQ=255)
                                                                                                                           aaGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCt                        >  2:580023/1‑151 (MQ=255)
                                                                                                                           aaGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCt                        <  1:3718956/151‑1 (MQ=255)
                                                                                                                            aGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTa                       <  1:4783451/151‑1 (MQ=255)
                                                                                                                            aGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTa                       >  1:3516687/1‑151 (MQ=255)
                                                                                                                                ccTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACAcc                   <  1:3197128/151‑1 (MQ=255)
                                                                                                                                ccTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACAcc                   >  1:4480364/1‑151 (MQ=255)
                                                                                                                                  tGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCa                 >  1:597688/1‑151 (MQ=255)
                                                                                                                                  tGGAAGACCTGGCGAAGTGCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCa                 <  2:1190935/151‑1 (MQ=255)
                                                                                                                                   ggAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAg                <  2:2274468/151‑1 (MQ=255)
                                                                                                                                    gAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGa               >  1:5111146/1‑151 (MQ=255)
                                                                                                                                     aaGACCTGGCGACGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGaa              <  1:841239/151‑1 (MQ=255)
                                                                                                                                     aaGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGaa              >  2:1940169/1‑151 (MQ=255)
                                                                                                                                      aGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGCGGTCGGCTACCCCCCGAAc             >  2:2849620/1‑151 (MQ=255)
                                                                                                                                       gACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACg            <  2:1803786/151‑1 (MQ=255)
                                                                                                                                           tGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGCCTACACCCAGAACGatat        >  2:853331/1‑151 (MQ=255)
                                                                                                                                             gCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCg      >  1:1979650/1‑151 (MQ=255)
                                                                                                                                               gAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGac    >  1:579366/1‑151 (MQ=255)
                                                                                                                                                 aGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGACTACACCCAGAACGATATCGacac  >  2:4824552/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AGCGTCGATGCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTACCCCTTCACCGGCAAGAACGATCTGCGCGACAACTACCCCTTCGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGCGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACAC  >  CP009974/5007133‑5007428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCAGCGTCGATGCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTACCCCTTCACCGGCAAGAACGATCTGCGCGACAACTACCCCTTCGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGCGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTG  >  CP009974/5007131‑5007431
                                                                                                                                                      |                                                                                                                                                      
CCAGCGTCGATGCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTT                                                                                                                                                        >  SRR10918231.2895326/1‑151 (MQ=60)
 CAGCGTCGATGCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTC                                                                                                                                                       >  SRR10918231.289888/1‑151 (MQ=60)
 CAGCGTCGATGCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTC                                                                                                                                                       >  SRR10918231.15800/1‑151 (MQ=60)
   GCGTCGATGCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCC                                                                                                                                                     >  SRR10918231.4262342/1‑151 (MQ=60)
    CGTCGATGCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCC                                                                                                                                                    <  SRR10918231.66481/151‑1 (MQ=60)
     GTCGATGCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCT                                                                                                                                                   >  SRR10918231.2670524/1‑151 (MQ=60)
      TCGATGCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTT                                                                                                                                                  >  SRR10918231.3091056/1‑151 (MQ=60)
         ATGCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCAC                                                                                                                                               >  SRR10918231.665302/1‑151 (MQ=60)
           GCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCG                                                                                                                                             <  SRR10918231.3019517/151‑1 (MQ=60)
           GCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCG                                                                                                                                             >  SRR10918231.648785/1‑151 (MQ=60)
           GCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCG                                                                                                                                             <  SRR10918231.4140865/151‑1 (MQ=60)
           GCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCG                                                                                                                                             >  SRR10918231.1523330/1‑151 (MQ=60)
           GCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCG                                                                                                                                             >  SRR10918231.2654513/1‑151 (MQ=60)
            CCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGG                                                                                                                                            <  SRR10918231.4174931/151‑1 (MQ=60)
             CCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGC                                                                                                                                           >  SRR10918231.2016170/1‑151 (MQ=60)
                GCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAG                                                                                                                                        >  SRR10918231.4401260/1‑151 (MQ=60)
                 CGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGA                                                                                                                                       <  SRR10918231.4479427/151‑1 (MQ=60)
                 CGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGA                                                                                                                                       <  SRR10918231.3153366/151‑1 (MQ=60)
                  GCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAA                                                                                                                                      >  SRR10918231.2089501/1‑151 (MQ=60)
                   CCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAAC                                                                                                                                     <  SRR10918231.4211582/151‑1 (MQ=60)
                    CAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACG                                                                                                                                    <  SRR10918231.3713098/151‑1 (MQ=60)
                    CAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACG                                                                                                                                    >  SRR10918231.1401827/1‑151 (MQ=60)
                       CACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACC                                                                                                                                 >  SRR10918231.4241776/1‑151 (MQ=60)
                         CCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTG                                                                                                                               >  SRR10918231.363582/1‑151 (MQ=60)
                         CCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTG                                                                                                                               <  SRR10918231.1041174/151‑1 (MQ=60)
                         CCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTG                                                                                                                               <  SRR10918231.954609/151‑1 (MQ=60)
                          CAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGC                                                                                                                              <  SRR10918231.3710524/151‑1 (MQ=60)
                          CAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGC                                                                                                                              >  SRR10918231.4456869/1‑151 (MQ=60)
                            GCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGC                                                                                                                            >  SRR10918231.785015/1‑151 (MQ=60)
                            GCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGC                                                                                                                            <  SRR10918231.1600775/151‑1 (MQ=60)
                              TGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGA                                                                                                                          >  SRR10918231.3117964/1‑151 (MQ=60)
                               GGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAAGGACCTGCGCGAC                                                                                                                         >  SRR10918231.1479333/1‑151 (MQ=60)
                               GGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGAC                                                                                                                         >  SRR10918231.5252048/1‑151 (MQ=60)
                               GGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGAC                                                                                                                         >  SRR10918231.725030/1‑151 (MQ=60)
                                GAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACA                                                                                                                        <  SRR10918231.77250/151‑1 (MQ=60)
                                  GCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAAC                                                                                                                      <  SRR10918231.4974367/151‑1 (MQ=60)
                                    GTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGGCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTG                                                                                                                    >  SRR10918231.2294891/1‑151 (MQ=60)
                                        GCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCC                                                                                                                >  SRR10918231.2083484/1‑151 (MQ=60)
                                        GCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCC                                                                                                                <  SRR10918231.4826468/151‑1 (MQ=60)
                                        GCGCTGGAGCCCGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCC                                                                                                                <  SRR10918231.4826430/151‑1 (MQ=60)
                                         CGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCT                                                                                                               >  SRR10918231.3490755/1‑151 (MQ=60)
                                          GCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTC                                                                                                              >  SRR10918231.2660704/1‑151 (MQ=60)
                                          GCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTA                                                                                                              <  SRR10918231.1481610/151‑1 (MQ=60)
                                            gggggggtggggggcgggggggggggtgtggggttgtggcggcggggggttggggagtagggggcgggccctggtggcctcgagtggCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACG                                                                                                            <  SRR10918231.2697570/64‑1 (MQ=60)
                                            GGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACG                                                                                                            <  SRR10918231.2167032/151‑1 (MQ=60)
                                               AGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCA                                                                                                         <  SRR10918231.3241066/151‑1 (MQ=60)
                                                GCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCAT                                                                                                        >  SRR10918231.1711979/1‑151 (MQ=60)
                                                GCCTGAAGCACGCCTACGACAATGTGCCGTTATACCGCCAGCGCTTTGCCGAGTACGGCCCCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGCTCCCCTTCACCGGCACGAACGACCTGCGCGACCCCTACCCCTACGGCAT                                                                                                        >  SRR10918231.2896263/1‑151 (MQ=60)
                                                   TGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTT                                                                                                     <  SRR10918231.2602767/151‑1 (MQ=60)
                                                   TGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTT                                                                                                     <  SRR10918231.3106508/151‑1 (MQ=60)
                                                    GAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTT                                                                                                    >  SRR10918231.2363659/1‑151 (MQ=60)
                                                    GAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGCTT                                                                                                    >  SRR10918231.2837712/1‑151 (MQ=60)
                                                        CACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCG                                                                                                >  SRR10918231.2357047/1‑151 (MQ=60)
                                                         ACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGT                                                                                               >  SRR10918231.176617/1‑151 (MQ=60)
                                                           GCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGC                                                                                             <  SRR10918231.3251864/151‑1 (MQ=60)
                                                              TACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCC                                                                                          >  SRR10918231.3705501/1‑151 (MQ=60)
                                                               ACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCTCCA                                                                                         >  SRR10918231.184250/1‑151 (MQ=60)
                                                               ACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCA                                                                                         >  SRR10918231.184260/1‑151 (MQ=60)
                                                                   CAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAA                                                                                     >  SRR10918231.3909573/1‑151 (MQ=60)
                                                                    AATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAG                                                                                    >  SRR10918231.3534750/1‑151 (MQ=60)
                                                                          CCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCCTGTTTGCCGTGCCCCCGGAAGAAGTGG                                                                              >  SRR10918231.4240809/1‑151 (MQ=60)
                                                                            GTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTG                                                                            <  SRR10918231.3019042/151‑1 (MQ=60)
                                                                             TTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGC                                                                           >  SRR10918231.1028629/1‑151 (MQ=60)
                                                                             TTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGC                                                                           <  SRR10918231.3885836/151‑1 (MQ=60)
                                                                             TTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGC                                                                           <  SRR10918231.187825/151‑1 (MQ=60)
                                                                              TGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCG                                                                          >  SRR10918231.1492731/1‑151 (MQ=60)
                                                                               GTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGC                                                                         >  SRR10918231.2195164/1‑151 (MQ=60)
                                                                                TACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCC                                                                        >  SRR10918231.3436756/1‑151 (MQ=60)
                                                                                 ACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCT                                                                       >  SRR10918231.2931006/1‑151 (MQ=60)
                                                                                   CGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGC                                                                     >  SRR10918231.1761365/1‑151 (MQ=60)
                                                                                   CGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGC                                                                     >  SRR10918231.4941755/1‑151 (MQ=60)
                                                                                   CGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGC                                                                     >  SRR10918231.3262533/1‑151 (MQ=60)
                                                                                   CGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGC                                                                     <  SRR10918231.4171988/151‑1 (MQ=60)
                                                                                     CCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCAT                                                                   <  SRR10918231.3755816/151‑1 (MQ=60)
                                                                                     CCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCAT                                                                   >  SRR10918231.1811986/1‑151 (MQ=60)
                                                                                      ctggagttcagacgtgtgctcttccgatCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATG                                                                  <  SRR10918231.2728733/123‑1 (MQ=60)
                                                                                        GCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCC                                                                >  SRR10918231.5172768/1‑151 (MQ=60)
                                                                                          GCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTC                                                              <  SRR10918231.2748962/151‑1 (MQ=60)
                                                                                           CTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCC                                                             >  SRR10918231.2128025/1‑151 (MQ=60)
                                                                                                  GAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCA                                                      >  SRR10918231.3126951/1‑151 (MQ=60)
                                                                                                    GTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACC                                                    >  SRR10918231.3179678/1‑151 (MQ=60)
                                                                                                    GTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACC                                                    >  SRR10918231.2691425/1‑151 (MQ=60)
                                                                                                        GGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCG                                                <  SRR10918231.3530125/151‑1 (MQ=60)
                                                                                                         GCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGG                                               <  SRR10918231.1301044/151‑1 (MQ=60)
                                                                                                                CCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCG                                        >  SRR10918231.478698/1‑151 (MQ=60)
                                                                                                                 CCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGA                                       >  SRR10918231.4202024/1‑151 (MQ=60)
                                                                                                                   TGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACG                                     <  SRR10918231.3135629/151‑1 (MQ=60)
                                                                                                                     ATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGT                                   <  SRR10918231.466010/151‑1 (MQ=60)
                                                                                                                      TGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTG                                  <  SRR10918231.863889/151‑1 (MQ=60)
                                                                                                                      TGACCTCAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTG                                  >  SRR10918231.58709/1‑151 (MQ=60)
                                                                                                                            CAAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGC                            >  SRR10918231.1675223/1‑151 (MQ=60)
                                                                                                                             AAGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCT                           <  SRR10918231.3736016/151‑1 (MQ=60)
                                                                                                                              AGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTA                          <  SRR10918231.4806390/151‑1 (MQ=60)
                                                                                                                              AGTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTA                          >  SRR10918231.3532727/1‑151 (MQ=60)
                                                                                                                               GTGCCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTAC                         <  SRR10918231.4824910/151‑1 (MQ=60)
                                                                                                                                  CCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACC                      <  SRR10918231.3211678/151‑1 (MQ=60)
                                                                                                                                  CCTGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACC                      >  SRR10918231.4501665/1‑151 (MQ=60)
                                                                                                                                    TGGAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCA                    >  SRR10918231.599892/1‑151 (MQ=60)
                                                                                                                                      GAAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGA                  >  SRR10918231.5136158/1‑151 (MQ=60)
                                                                                                                                       AAGACCTGGCGACGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAA                 <  SRR10918231.844733/151‑1 (MQ=60)
                                                                                                                                       AAGACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAC                 <  SRR10918231.2564528/151‑1 (MQ=60)
                                                                                                                                         GACCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACG               >  SRR10918231.1819137/1‑151 (MQ=60)
                                                                                                                                           CCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGNCTCCAGTGGCACCACCTGCAAGTNGANGGTNGTCNGCNACANCCANAANGAN             >  SRR10918231.995797/1‑151 (MQ=60)
                                                                                                                                           CCTGGCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGAT             <  SRR10918231.2660714/151‑1 (MQ=60)
                                                                                                                                               GCGAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCG         >  SRR10918231.1988565/1‑151 (MQ=60)
                                                                                                                                                 GAAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGAC       >  SRR10918231.581480/1‑151 (MQ=60)
                                                                                                                                                  AAGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGCGGTCGGCTACACCCAGAACCATATCGACC      >  SRR10918231.3928618/1‑151 (MQ=60)
                                                                                                                                                   AGTTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACAC     >  SRR10918231.2820970/1‑151 (MQ=60)
                                                                                                                                                     TTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCT   >  SRR10918231.650227/1‑151 (MQ=60)
                                                                                                                                                     TTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCT   <  SRR10918231.2326729/151‑1 (MQ=60)
                                                                                                                                                     TTCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCT   <  SRR10918231.2326558/151‑1 (MQ=60)
                                                                                                                                                      TCCCCTTCACCGGCAAGAACGACCTGCGCGACAACTACCCCTACGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGTGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTG  <  SRR10918231.3973680/151‑1 (MQ=60)
                                                                                                                                                      |                                                                                                                                                      
CCAGCGTCGATGCCCTGCGCCAGCACCAGCTGGAGCGTCTGCGCTGGAGCCTGAAGCACGCCTACGACAATGTGCCGTTGTACCGCCAGCGCTTTGCCGAGTACGGCACCCACCCTGATGACCTCAAGTGCCTGGAAGACCTGGCGAAGTACCCCTTCACCGGCAAGAACGATCTGCGCGACAACTACCCCTTCGGCATGTTTGCCGTGCCCCAGGAAGAAGTGGTGCGCCTGCATGCCTCCAGCGGCACCACCGGCAAGCCGACGGTGGTCGGCTACACCCAGAACGATATCGACACCTG  >  CP009974/5007131‑5007431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑