Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 241,624 G→T 35.4% V223V (GTC→GTA)  yafK ← L,D‑transpeptidase‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273241,6240GT35.4% 467.6 / 273.3 282V223V (GTC→GTA) yafKL,D‑transpeptidase‑related protein
Reads supporting (aligned to +/- strand):  ref base G (100/82);  new base T (47/53);  total (147/135)
Fisher's exact test for biased strand distribution p-value = 2.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 286 total aligned reads displayed.

ATGTGGTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTA  >  CP009273/241475‑241773
                                                                                                                                                     |                                                                                                                                                     
atgTGGTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTg                                                                                                                                                       <  1:1322339/150‑1 (MQ=255)
        aCGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTa                                                                                                                                               >  2:4337498/1‑150 (MQ=255)
          ggTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTTACCACGTAAc                                                                                                                                             >  1:147201/1‑150 (MQ=255)
           gTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTTACCACGTAACg                                                                                                                                            <  2:392478/150‑1 (MQ=255)
            tGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGa                                                                                                                                           <  1:692874/150‑1 (MQ=255)
                tGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTTACCACGTAACGACCAt                                                                                                                                       >  1:1761605/1‑150 (MQ=255)
                   aaCACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTTACCACGTAACGACCATTGa                                                                                                                                    >  1:990882/1‑150 (MQ=255)
                    acacAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGAc                                                                                                                                   >  1:2654381/1‑150 (MQ=255)
                         gCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAg                                                                                                                              <  1:1045907/150‑1 (MQ=255)
                         gCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAg                                                                                                                              <  1:341033/150‑1 (MQ=255)
                               gACGGGCAATGAAACCTGGCAAAAGATTATGCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTTACCACGTAACGACCATTGACCACAGAAACgg                                                                                                                        >  1:17121/1‑150 (MQ=255)
                               gACGGGCAATGAAACCTGGCAAAAGATTATGCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACgg                                                                                                                        >  1:272539/1‑150 (MQ=255)
                                aCGGGCAATGAAACCTGGCAAAAGATTATGCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGt                                                                                                                       >  2:1166152/1‑150 (MQ=255)
                                 cGGGCAATGAAACCTGGCAAAAGATTATGCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTTACCACGTAACGACCATTGACCACAGAAACGGtt                                                                                                                      <  2:919122/150‑1 (MQ=255)
                                 cGGGCAATGAAACCTGGCAAAAGATTATGCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGtt                                                                                                                      >  2:4048382/1‑150 (MQ=255)
                                  gggCAATGAAACCTGGCAAAAGATTATGCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTTCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTtg                                                                                                                     <  1:2766547/150‑1 (MQ=255)
                                  gggCAATGAAACCTGGCAAAAGATTATGCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTtg                                                                                                                     >  1:3426286/1‑150 (MQ=255)
                                      aaTGAAACCTGGCAAAAGATTATGCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTTACCACGTAACGACCATTGACCACAGAAACGGTtggtgg                                                                                                                 <  1:3318295/150‑1 (MQ=255)
                                         gAAACCTGGCAAAAGATTATGCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTTACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCtt                                                                                                              >  2:3809008/1‑150 (MQ=255)
                                         gAAACCTGGCAAAAGATTATGCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTTACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCtt                                                                                                              <  2:3244849/150‑1 (MQ=255)
                                         gAAACCTGGCAAAAGATTATGCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCtt                                                                                                              <  1:2340270/150‑1 (MQ=255)
                                          aaaCCTGGCAAAAGATTATGCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTTACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTa                                                                                                             >  2:4522774/1‑150 (MQ=255)
                                          aaaCCTGGCAAAAGATTATGCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTTACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTa                                                                                                             >  2:3971300/1‑150 (MQ=255)
                                            aCCTGGCAAAAGATTATGCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTTACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACg                                                                                                           <  2:1406914/150‑1 (MQ=255)
                                             ccTGGCAAAAGATTATGCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACgg                                                                                                          >  2:1155222/1‑150 (MQ=255)
                                                    aaaGATTATGCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCt                                                                                                   <  1:3233584/150‑1 (MQ=255)
                                                     aaGATTATGCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTc                                                                                                  <  1:3949801/150‑1 (MQ=255)
                                                        aTTATGCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTTACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCaaa                                                                                               <  2:85140/150‑1 (MQ=255)
                                                         ttATGCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTTACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAag                                                                                              >  2:2797615/1‑150 (MQ=255)
                                                            tGCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAagtag                                                                                           <  2:3052667/150‑1 (MQ=255)
                                                             gCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAagtagt                                                                                          <  2:4182710/150‑1 (MQ=255)
                                                              ccAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTc                                                                                         >  2:2427509/1‑150 (MQ=255)
                                                               cAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTTACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCg                                                                                        >  1:3843758/1‑150 (MQ=255)
                                                                aGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTTACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGt                                                                                       <  1:432896/150‑1 (MQ=255)
                                                                aGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGt                                                                                       >  1:1089770/1‑150 (MQ=255)
                                                                aGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGt                                                                                       >  1:4073857/1‑150 (MQ=255)
                                                                    gAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTTACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAAcc                                                                                   >  2:2382321/1‑150 (MQ=255)
                                                                     aaTGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAAccc                                                                                  <  1:2980996/150‑1 (MQ=255)
                                                                      aTGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCg                                                                                 >  1:2088783/1‑150 (MQ=255)
                                                                       tGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTTACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCgg                                                                                >  1:520511/1‑150 (MQ=255)
                                                                        ggCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTTACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGc                                                                               >  1:4547464/1‑150 (MQ=255)
                                                                        ggCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGc                                                                               >  2:4603062/1‑150 (MQ=255)
                                                                           gcTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTc                                                                            <  1:4609357/150‑1 (MQ=255)
                                                                           gcTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTc                                                                            >  2:1495100/1‑150 (MQ=255)
                                                                            cTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCa                                                                           >  2:218316/1‑150 (MQ=255)
                                                                            cTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCa                                                                           <  2:1103856/150‑1 (MQ=255)
                                                                             ttATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAg                                                                          >  2:3858552/1‑150 (MQ=255)
                                                                             ttATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAg                                                                          <  2:321253/150‑1 (MQ=255)
                                                                               aTTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTTACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGtt                                                                        <  1:801355/150‑1 (MQ=255)
                                                                                ttttGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTg                                                                       <  1:2192846/150‑1 (MQ=255)
                                                                                   tGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTc                                                                    <  1:1270726/150‑1 (MQ=255)
                                                                                    gCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTcc                                                                   >  2:576514/1‑150 (MQ=255)
                                                                                      cTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCa                                                                 >  1:3680556/1‑150 (MQ=255)
                                                                                      cTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCa                                                                 >  1:4003002/1‑150 (MQ=255)
                                                                                       tCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAg                                                                >  2:3360982/1‑150 (MQ=255)
                                                                                        cGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGa                                                               >  2:1288303/1‑150 (MQ=255)
                                                                                           ggAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGt                                                            >  1:2611628/1‑150 (MQ=255)
                                                                                           ggAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGt                                                            >  2:4525485/1‑150 (MQ=255)
                                                                                             aGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTTACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTcc                                                          >  1:3030017/1‑150 (MQ=255)
                                                                                              gCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTTACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCt                                                         <  1:2001570/150‑1 (MQ=255)
                                                                                              gCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCt                                                         <  2:511821/150‑1 (MQ=255)
                                                                                               cGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTTACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCtt                                                        >  1:497268/1‑150 (MQ=255)
                                                                                                 tgtAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTTACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTaa                                                      <  1:2796123/150‑1 (MQ=255)
                                                                                                  gtAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTaaa                                                     <  2:1720433/150‑1 (MQ=255)
                                                                                                    aGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTTACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGt                                                   <  2:349008/150‑1 (MQ=255)
                                                                                                     gTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAggt                                                  >  2:1565461/1‑148 (MQ=255)
                                                                                                     gTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGtt                                                  <  1:3202138/150‑1 (MQ=255)
                                                                                                     gTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGtt                                                  >  2:340911/1‑150 (MQ=255)
                                                                                                        tGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGa                                               <  2:1666361/150‑1 (MQ=255)
                                                                                                        tGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGa                                               <  2:63809/150‑1 (MQ=255)
                                                                                                         gATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGaa                                              <  1:1585447/150‑1 (MQ=255)
                                                                                                           tGCCAGTTGTGTCTGCACAACTTCGTGGCTTAACGGCTTGCTTACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCTAGAAGTCCTTAAAGTTGGAata                                            >  2:4588087/1‑150 (MQ=255)
                                                                                                               aGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTa                                        >  1:2259256/1‑150 (MQ=255)
                                                                                                                   gtgGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGgc                                    <  2:2200372/150‑1 (MQ=255)
                                                                                                                    tgGCTGCACAACTTCGTGGCTTAACGGCTTGCTTACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGgcg                                   <  2:2558369/150‑1 (MQ=255)
                                                                                                                    tgGCTGCACAACTTCGTGGCTTAACGGCTTGCTTACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGgcg                                   <  2:1587276/150‑1 (MQ=255)
                                                                                                                    tgGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGgcg                                   >  2:2183220/1‑150 (MQ=255)
                                                                                                                       cTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCtt                                <  2:1325756/150‑1 (MQ=255)
                                                                                                                        tGCACAACTTCGTGGCTTAACGGCTTGCTTACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTc                               <  2:2847344/150‑1 (MQ=255)
                                                                                                                        tGCACAACTTCGTGGCTTAACGGCTTGCTTACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTc                               >  2:1855146/1‑150 (MQ=255)
                                                                                                                        tGCACAACTTCGTGGCTTAACGGCTTGCTTACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTc                               <  2:3621650/150‑1 (MQ=255)
                                                                                                                            caACTTCGTGGCTTAACGGCTTGCTTACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCatat                           >  1:3459100/1‑150 (MQ=255)
                                                                                                                            caACTTCGTGGCTTAACGGCTTGCTTACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCatat                           >  1:2870567/1‑150 (MQ=255)
                                                                                                                             aaCTTCGTGGCTTAACGGCTTGCTTACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAACATTTATGGCGCTTCATAtt                          >  2:4300809/1‑150 (MQ=255)
                                                                                                                             aaCTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATAtt                          >  1:2328308/1‑150 (MQ=255)
                                                                                                                             aaCTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATAtt                          >  1:2791631/1‑150 (MQ=255)
                                                                                                                                   gTGGCTTAACGGCTTGCTTACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTc                    <  2:1761605/150‑1 (MQ=255)
                                                                                                                                   gTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTGCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTc                    >  1:1026775/1‑150 (MQ=255)
                                                                                                                                    tGGCTTAACGGCTTGCTTACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCg                   <  1:1455675/150‑1 (MQ=255)
                                                                                                                                       cTTAACGGCTTGCTTACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGGTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTc                >  2:233489/1‑150 (MQ=255)
                                                                                                                                       cTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTc                >  1:905267/1‑150 (MQ=255)
                                                                                                                                        ttAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCa               <  1:1833562/150‑1 (MQ=255)
                                                                                                                                         tAACGGCTTGCTTACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCAt              >  2:2955625/1‑150 (MQ=255)
                                                                                                                                         tAACGGCTTGCTTACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCAt              <  2:147183/150‑1 (MQ=255)
                                                                                                                                          aaCGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATg             >  1:3576416/1‑150 (MQ=255)
                                                                                                                                            cGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCg           <  1:2680355/150‑1 (MQ=255)
                                                                                                                                             ggCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCgg          >  2:2227105/1‑150 (MQ=255)
                                                                                                                                             ggCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCgg          >  1:3247862/1‑150 (MQ=255)
                                                                                                                                              gCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGa         <  1:4170843/150‑1 (MQ=255)
                                                                                                                                                     gACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTa  <  2:4003002/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATGTGGTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGACCATTGACCACAGAAACGGTTGGTGGCTTACGGGTTTGCTCAAAGTAGTCGTAACCCGGCTTCAGTTGCTCCCAGAAGTCCTTAAAGTTGGAATATTTATGGCGCTTCATATTGGCGTCGGTCATGCGGAACGGGTA  >  CP009273/241475‑241773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A