Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 262,114 C→A 90.5% D51Y (GAT→TAT)  yafY ← lipoprotein, inner membrane; overproduction stimulates degP expression; CP4‑6 prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273262,1140CA90.5% 725.9 / 71.0 262D51Y (GAT→TAT) yafYlipoprotein, inner membrane; overproduction stimulates degP expression; CP4‑6 prophage
Reads supporting (aligned to +/- strand):  ref base C (16/9);  new base A (107/130);  total (123/139)
Fisher's exact test for biased strand distribution p-value = 9.19e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 271 total aligned reads displayed.

TTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGTTTTACGTT  >  CP009273/261966‑262260
                                                                                                                                                    |                                                                                                                                                  
  catcaGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACataatc                                                                                                                                                 >  1:2478915/1‑150 (MQ=255)
  catcaGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACataatc                                                                                                                                                 >  2:3739541/1‑150 (MQ=255)
  catcaGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACataatc                                                                                                                                                 <  2:1558959/150‑1 (MQ=255)
   atcaGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATcc                                                                                                                                                >  1:4125502/1‑150 (MQ=255)
    tcaGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCt                                                                                                                                               <  1:2410564/150‑1 (MQ=255)
    tcaGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCt                                                                                                                                               <  1:3091209/150‑1 (MQ=255)
     caGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCtg                                                                                                                                              >  2:1247236/1‑150 (MQ=255)
     caGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATACTTCGCTGACATCAACCtg                                                                                                                                              >  1:3730533/1‑150 (MQ=255)
      aGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAATTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCtgg                                                                                                                                             <  2:2352012/150‑1 (MQ=255)
       gTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCtggt                                                                                                                                            <  2:2969280/150‑1 (MQ=255)
       gTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCtggt                                                                                                                                            <  1:2806233/150‑1 (MQ=255)
              gACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGAc                                                                                                                                     <  1:2649436/150‑1 (MQ=255)
                cGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACca                                                                                                                                   <  2:4261498/150‑1 (MQ=255)
                  gTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACcaca                                                                                                                                 >  2:4084805/1‑150 (MQ=255)
                  gTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACcaca                                                                                                                                 <  2:314595/150‑1 (MQ=255)
                  gTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACcaca                                                                                                                                 >  2:664801/1‑150 (MQ=255)
                   ttttttCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACAt                                                                                                                                >  1:1838100/1‑150 (MQ=255)
                   ttttttCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACAt                                                                                                                                >  1:634662/1‑150 (MQ=255)
                   ttttttCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACAt                                                                                                                                >  1:3275549/1‑150 (MQ=255)
                    tttttCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATc                                                                                                                               <  1:1007711/150‑1 (MQ=255)
                    tttttCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATc                                                                                                                               <  1:1678873/150‑1 (MQ=255)
                    tttttCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATc                                                                                                                               <  1:1252006/150‑1 (MQ=255)
                       ttCCAGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCTGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACt                                                                                                                            >  2:4096304/1‑150 (MQ=255)
                          cTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGaa                                                                                                                         <  2:4182781/150‑1 (MQ=255)
                          cTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGaa                                                                                                                         >  1:3381984/1‑150 (MQ=255)
                             cATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAAcg                                                                                                                      <  2:2888143/150‑1 (MQ=255)
                             cATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAAcg                                                                                                                      <  2:2218003/150‑1 (MQ=255)
                              atatCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAAcgc                                                                                                                     >  2:614534/1‑150 (MQ=255)
                                atCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCgg                                                                                                                   <  2:4290474/150‑1 (MQ=255)
                                atCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCgg                                                                                                                   >  1:3802734/1‑150 (MQ=255)
                                 tCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGt                                                                                                                  <  2:2207058/150‑1 (MQ=255)
                                      gAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGt                                                                                                             >  2:1930467/1‑150 (MQ=255)
                                      gAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGt                                                                                                             >  2:2767784/1‑150 (MQ=255)
                                      gAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGt                                                                                                             <  2:3639958/150‑1 (MQ=255)
                                      gAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGt                                                                                                             <  2:4001750/150‑1 (MQ=255)
                                       aGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTg                                                                                                            >  2:1425719/1‑150 (MQ=255)
                                          tagtagtaACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATACGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAgg                                                                                                         >  1:3519745/1‑150 (MQ=255)
                                          tagtagtaACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAgg                                                                                                         <  1:2186066/150‑1 (MQ=255)
                                           agtagtaACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGt                                                                                                        <  2:4631414/150‑1 (MQ=255)
                                            gtagtaACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTc                                                                                                       >  2:4505554/1‑150 (MQ=255)
                                                taACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCttc                                                                                                   >  1:3870407/1‑149 (MQ=255)
                                                taACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTa                                                                                                   >  1:506614/1‑150 (MQ=255)
                                                 aaCTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTaa                                                                                                  >  1:516558/1‑150 (MQ=255)
                                                 aaCTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTaa                                                                                                  >  1:211508/1‑150 (MQ=255)
                                                    tGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAAtt                                                                                               <  2:4333660/150‑1 (MQ=255)
                                                       cATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTAc                                                                                            >  1:664934/1‑150 (MQ=255)
                                                       cATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTAc                                                                                            <  2:1013449/150‑1 (MQ=255)
                                                       cATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTAc                                                                                            >  1:1550174/1‑150 (MQ=255)
                                                        aTCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACt                                                                                           <  2:3585423/150‑1 (MQ=255)
                                                         tCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTg                                                                                          <  1:4379981/150‑1 (MQ=255)
                                                          ccTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGa                                                                                         >  2:3920711/1‑150 (MQ=255)
                                                             cGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATgg                                                                                      <  1:4506151/150‑1 (MQ=255)
                                                                    aTCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGATGACCACATAACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAg                                                                               >  1:3196174/1‑150 (MQ=255)
                                                                         gCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGtcat                                                                          <  1:1066845/150‑1 (MQ=255)
                                                                           gACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGtcatca                                                                        <  1:2989870/150‑1 (MQ=255)
                                                                           gACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGtcatca                                                                        <  1:4237158/150‑1 (MQ=255)
                                                                              tGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAACCGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACtt                                                                     <  2:4378062/150‑1 (MQ=255)
                                                                                gCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCTCATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCt                                                                   <  2:829499/150‑1 (MQ=255)
                                                                                      aCGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATc                                                             <  2:3094126/150‑1 (MQ=255)
                                                                                      aCGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATc                                                             <  1:3737809/150‑1 (MQ=255)
                                                                                       cGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCg                                                            <  2:2506105/150‑1 (MQ=255)
                                                                                        gTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGc                                                           <  1:229282/150‑1 (MQ=255)
                                                                                          aaaaCCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCAt                                                         >  1:251130/1‑150 (MQ=255)
                                                                                          aaaaCCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGATGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCAt                                                         >  1:2163882/1‑150 (MQ=255)
                                                                                           aaaCCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAACCGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATg                                                        >  2:2001732/1‑150 (MQ=255)
                                                                                              ccTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGa                                                     <  2:1485727/150‑1 (MQ=255)
                                                                                               cTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGAt                                                    <  2:3895870/150‑1 (MQ=255)
                                                                                                  cAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATgag                                                 >  2:687918/1‑150 (MQ=255)
                                                                                                   aaGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATgaga                                                >  2:764973/1‑150 (MQ=255)
                                                                                                      gTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAAc                                             >  2:1128398/1‑150 (MQ=255)
                                                                                                      gTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAAc                                             <  2:2165090/150‑1 (MQ=255)
                                                                                                          gACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACagag                                         <  1:1223875/150‑1 (MQ=255)
                                                                                                              ggATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAgtggt                                     <  2:3753852/150‑1 (MQ=255)
                                                                                                              ggATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAgtggt                                     <  1:4295111/150‑1 (MQ=255)
                                                                                                                aTAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGtggtgg                                   >  2:3396463/1‑150 (MQ=255)
                                                                                                                 tAAGTAGTCACCCTGGGACCATTCGTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGAGAGGTCCTTAACTGTACTGCGGGCCTTCAGGTCATCACTTCTGTCATAGCATGAGATGAGAACCAGACTGGTGGc                                  <  2:3721923/150‑1 (MQ=255)
                                                                                                                    gtagtCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAAc                               >  2:1732379/1‑150 (MQ=255)
                                                                                                                     tagtCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAAcc                              >  1:3125941/1‑150 (MQ=255)
                                                                                                                            ccTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCca                       >  1:3075852/1‑150 (MQ=255)
                                                                                                                            ccTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCca                       >  2:2301816/1‑150 (MQ=255)
                                                                                                                            ccTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCca                       >  2:4575381/1‑150 (MQ=255)
                                                                                                                                tgACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCcagcag                   >  1:4050270/1‑150 (MQ=255)
                                                                                                                                 gACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCaga                  >  2:299982/1‑150 (MQ=255)
                                                                                                                                  aCCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCagag                 >  2:1072479/1‑150 (MQ=255)
                                                                                                                                  aCCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCagag                 <  2:2478915/150‑1 (MQ=255)
                                                                                                                                   ccATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAgg                <  2:1282615/150‑1 (MQ=255)
                                                                                                                                    cATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGc               >  1:2239700/1‑150 (MQ=255)
                                                                                                                                     aTTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCa              <  2:1639441/150‑1 (MQ=255)
                                                                                                                                       tcttcGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAgt            <  1:1862872/150‑1 (MQ=255)
                                                                                                                                        cttcGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAgtg           <  1:3956773/150‑1 (MQ=255)
                                                                                                                                        cttcGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAgtg           <  1:771461/150‑1 (MQ=255)
                                                                                                                                         ttcGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAgtgt          >  1:10481/1‑150 (MQ=255)
                                                                                                                                          tcGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGtt         >  1:671536/1‑150 (MQ=255)
                                                                                                                                           cGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGttt        >  1:1104317/1‑150 (MQ=255)
                                                                                                                                           cGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGttt        <  1:2939380/150‑1 (MQ=255)
                                                                                                                                            gCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGtttt       <  2:2633433/150‑1 (MQ=255)
                                                                                                                                             cTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGTTTTa      >  2:1348888/1‑150 (MQ=255)
                                                                                                                                             cTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGTTTTa      <  2:1838100/150‑1 (MQ=255)
                                                                                                                                              tGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGTTTTAc     <  2:3324756/150‑1 (MQ=255)
                                                                                                                                               gACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGTTTTACg    <  1:1613110/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGTTTTACGTT  >  CP009273/261966‑262260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGTTTTACGTT  >  CP009273/261966‑262260
                                                                                                                                                    |                                                                                                                                                  
TTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGTGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATCA                                                                                                                                                   <  SRR19973072.3212885/150‑1 (MQ=60)
TTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATCA                                                                                                                                                   <  SRR19973072.3212882/150‑1 (MQ=60)
  CATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATC                                                                                                                                                 >  SRR19973072.2484292/1‑150 (MQ=60)
   ATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCC                                                                                                                                                >  SRR19973072.4134158/1‑150 (MQ=60)
   ATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAATAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCC                                                                                                                                                >  SRR19973072.4396616/1‑150 (MQ=60)
    TCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCT                                                                                                                                               <  SRR19973072.2416110/150‑1 (MQ=60)
    TCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCT                                                                                                                                               <  SRR19973072.2415792/150‑1 (MQ=60)
    TCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCT                                                                                                                                               <  SRR19973072.3097723/150‑1 (MQ=60)
     CAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTG                                                                                                                                              >  SRR19973072.3738401/1‑150 (MQ=60)
     CAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTG                                                                                                                                              >  SRR19973072.3738402/1‑150 (MQ=60)
     CAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATACTTCGCTGACATCAACCTG                                                                                                                                              >  SRR19973072.3738385/1‑150 (MQ=60)
       GTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGT                                                                                                                                            <  SRR19973072.2812242/150‑1 (MQ=60)
        TGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTG                                                                                                                                           >  SRR19973072.2196817/1‑150 (MQ=60)
           CTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCT                                                                                                                                        <  SRR19973072.3836537/150‑1 (MQ=60)
           CTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCT                                                                                                                                        <  SRR19973072.4202988/150‑1 (MQ=60)
              GACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGAC                                                                                                                                     <  SRR19973072.2655115/150‑1 (MQ=60)
                   TTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACAT                                                                                                                                >  SRR19973072.1842094/1‑150 (MQ=60)
                   TTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACAT                                                                                                                                >  SRR19973072.3282501/1‑150 (MQ=60)
                   TTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACAT                                                                                                                                >  SRR19973072.636110/1‑150 (MQ=60)
                    TTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATC                                                                                                                               <  SRR19973072.1682545/150‑1 (MQ=60)
                    TTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATC                                                                                                                               <  SRR19973072.1254694/150‑1 (MQ=60)
                    TTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATC                                                                                                                               <  SRR19973072.1009908/150‑1 (MQ=60)
                      TTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCAC                                                                                                                             >  SRR19973072.2313391/1‑150 (MQ=60)
                       TTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACT                                                                                                                            <  SRR19973072.2417032/150‑1 (MQ=60)
                          CTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAA                                                                                                                         >  SRR19973072.3389135/1‑150 (MQ=60)
                          CTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAA                                                                                                                         <  SRR19973072.4270435/150‑1 (MQ=60)
                            GCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAAC                                                                                                                       <  SRR19973072.617718/150‑1 (MQ=60)
                            GCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAAC                                                                                                                       <  SRR19973072.617450/150‑1 (MQ=60)
                             CATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATACTCCTGGTGGCTGACCACATCACTGAAACG                                                                                                                      >  SRR19973072.2132937/1‑150 (MQ=60)
                              ATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGC                                                                                                                     <  SRR19973072.769749/150‑1 (MQ=60)
                                ATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGG                                                                                                                   >  SRR19973072.3810696/1‑150 (MQ=60)
                                  CCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTG                                                                                                                 <  SRR19973072.249468/150‑1 (MQ=60)
                                   CACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGG                                                                                                                >  SRR19973072.3437526/1‑150 (MQ=60)
                                      GAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGT                                                                                                             <  SRR19973072.2999657/150‑1 (MQ=60)
                                      GAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGT                                                                                                             <  SRR19973072.1814505/150‑1 (MQ=60)
                                      GAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGT                                                                                                             >  SRR19973072.2307004/1‑150 (MQ=60)
                                          TAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATACGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGG                                                                                                         >  SRR19973072.3527131/1‑150 (MQ=60)
                                          TAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGG                                                                                                         <  SRR19973072.2190819/150‑1 (MQ=60)
                                                TAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTC                                                                                                   >  SRR19973072.3878451/1‑150 (MQ=60)
                                                TAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTA                                                                                                   >  SRR19973072.3878553/1‑150 (MQ=60)
                                                TAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTA                                                                                                   >  SRR19973072.507830/1‑150 (MQ=60)
                                                TAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTA                                                                                                   >  SRR19973072.507819/1‑150 (MQ=60)
                                                TAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTA                                                                                                   >  SRR19973072.764270/1‑150 (MQ=60)
                                                 AACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAA                                                                                                  >  SRR19973072.517791/1‑150 (MQ=60)
                                                 AACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAA                                                                                                  >  SRR19973072.212036/1‑150 (MQ=60)
                                                  ACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAA                                                                                                 >  SRR19973072.3202875/1‑150 (MQ=60)
                                                  ACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAA                                                                                                 <  SRR19973072.1042180/150‑1 (MQ=60)
                                                       CATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTAC                                                                                            >  SRR19973072.1553461/1‑150 (MQ=60)
                                                       CATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTAC                                                                                            >  SRR19973072.666426/1‑150 (MQ=60)
                                                        ATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACT                                                                                           <  SRR19973072.1396665/150‑1 (MQ=60)
                                                         TCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTG                                                                                          <  SRR19973072.4389197/150‑1 (MQ=60)
                                                         TCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTG                                                                                          <  SRR19973072.4389187/150‑1 (MQ=60)
                                                          CCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGA                                                                                         <  SRR19973072.2552881/150‑1 (MQ=60)
                                                           CTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGAT                                                                                        <  SRR19973072.4314/150‑1 (MQ=60)
                                                            TCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATG                                                                                       >  SRR19973072.2169806/1‑150 (MQ=60)
                                                            TCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATG                                                                                       >  SRR19973072.1104767/1‑150 (MQ=60)
                                                            TCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATG                                                                                       <  SRR19973072.2518534/150‑1 (MQ=60)
                                                             CGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGG                                                                                      <  SRR19973072.4515666/150‑1 (MQ=60)
                                                              GCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGC                                                                                     >  SRR19973072.3776908/1‑150 (MQ=60)
                                                               CAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCC                                                                                    >  SRR19973072.1196448/1‑150 (MQ=60)
                                                                   CATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCA                                                                                >  SRR19973072.660540/1‑150 (MQ=60)
                                                                   CATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCA                                                                                >  SRR19973072.660430/1‑150 (MQ=60)
                                                                    ATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAG                                                                               <  SRR19973072.2164122/150‑1 (MQ=60)
                                                                    ATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATAACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAG                                                                               >  SRR19973072.4343920/1‑150 (MQ=60)
                                                                    ATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGATGACCACATAACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAG                                                                               >  SRR19973072.3202927/1‑150 (MQ=60)
                                                                         GCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCAT                                                                          <  SRR19973072.1069126/150‑1 (MQ=60)
                                                                         GCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCAT                                                                          <  SRR19973072.1069008/150‑1 (MQ=60)
                                                                         GCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCAT                                                                          <  SRR19973072.1068978/150‑1 (MQ=60)
                                                                           GACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCA                                                                        <  SRR19973072.2996241/150‑1 (MQ=60)
                                                                           GACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCA                                                                        <  SRR19973072.2754112/150‑1 (MQ=60)
                                                                           GACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCA                                                                        <  SRR19973072.4245981/150‑1 (MQ=60)
                                                                                GCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCT                                                                   <  SRR19973072.410265/150‑1 (MQ=60)
                                                                                     TACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCAT                                                              >  SRR19973072.3333349/1‑150 (MQ=60)
                                                                                      ACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATC                                                             <  SRR19973072.949889/150‑1 (MQ=60)
                                                                                      ACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATC                                                             <  SRR19973072.666782/150‑1 (MQ=60)
                                                                                      ACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATC                                                             <  SRR19973072.3745670/150‑1 (MQ=60)
                                                                                        GTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGC                                                           <  SRR19973072.229848/150‑1 (MQ=60)
                                                                                          AAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCAT                                                         >  SRR19973072.251734/1‑150 (MQ=60)
                                                                                          AAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGATGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCAT                                                         >  SRR19973072.2168598/1‑150 (MQ=60)
                                                                                          AAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGATGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCAT                                                         >  SRR19973072.2168637/1‑150 (MQ=60)
                                                                                             ACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCG                                                      <  SRR19973072.360665/150‑1 (MQ=60)
                                                                                                          GACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAG                                         <  SRR19973072.1688840/150‑1 (MQ=60)
                                                                                                          GACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAG                                         <  SRR19973072.1226491/150‑1 (MQ=60)
                                                                                                          GACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAG                                         <  SRR19973072.1226496/150‑1 (MQ=60)
                                                                                                           ACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGT                                        <  SRR19973072.2988051/150‑1 (MQ=60)
                                                                                                            CCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTG                                       <  SRR19973072.186371/150‑1 (MQ=60)
                                                                                                            CCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTG                                       <  SRR19973072.186335/150‑1 (MQ=60)
                                                                                                            CCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTG                                       <  SRR19973072.1453923/150‑1 (MQ=60)
                                                                                                              GGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGT                                     <  SRR19973072.4304065/150‑1 (MQ=60)
                                                                                                              GGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGT                                     <  SRR19973072.3961453/150‑1 (MQ=60)
                                                                                                               GATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTG                                    <  SRR19973072.4528292/150‑1 (MQ=60)
                                                                                                                   ttTAGTCAACATGTGACCATTCTTCGCTGACATAATCCGGGTGGCTGACCACATCACTGACACGCGGTGGGGTGAGGTCCTTAAATTTTCTGAGGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAA                                <  SRR19973072.4104889/148‑1 (MQ=60)
                                                                                                                   AGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAA                                >  SRR19973072.2016052/1‑150 (MQ=60)
                                                                                                                   AGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATAACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAA                                <  SRR19973072.4104908/150‑1 (MQ=60)
                                                                                                                     TAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACC                              >  SRR19973072.3132538/1‑150 (MQ=60)
                                                                                                                       GTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAG                            >  SRR19973072.3863120/1‑150 (MQ=60)
                                                                                                                           ACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCC                        >  SRR19973072.2245427/1‑150 (MQ=60)
                                                                                                                           ACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCC                        >  SRR19973072.2245041/1‑150 (MQ=60)
                                                                                                                            CCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCA                       >  SRR19973072.3082347/1‑150 (MQ=60)
                                                                                                                            CCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCA                       >  SRR19973072.3082479/1‑150 (MQ=60)
                                                                                                                            CCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCA                       >  SRR19973072.3082729/1‑150 (MQ=60)
                                                                                                                                TGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAG                   >  SRR19973072.4058772/1‑150 (MQ=60)
                                                                                                                                TGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAG                   <  SRR19973072.3461987/150‑1 (MQ=60)
                                                                                                                                 GACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGA                  >  SRR19973072.1101600/1‑150 (MQ=60)
                                                                                                                                    CATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACTCGGTGGGGTGAGGTCCTTAAATTTACTTATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGC               >  SRR19973072.1634195/1‑150 (MQ=60)
                                                                                                                                    CATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGC               >  SRR19973072.1634237/1‑150 (MQ=60)
                                                                                                                                    CATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGC               >  SRR19973072.2244538/1‑150 (MQ=60)
                                                                                                                                    CATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCAGGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGC               >  SRR19973072.353207/1‑150 (MQ=60)
                                                                                                                                     ATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCA              >  SRR19973072.2999461/1‑150 (MQ=60)
                                                                                                                                      TTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAG             <  SRR19973072.4012188/150‑1 (MQ=60)
                                                                                                                                       TCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGTGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAATCAGTGTGGTGGCAACCAGCGCCTGCAGAGGCAGT            <  SRR19973072.4241759/150‑1 (MQ=60)
                                                                                                                                       TCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGT            <  SRR19973072.1866892/150‑1 (MQ=60)
                                                                                                                                        CTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTG           <  SRR19973072.3965039/150‑1 (MQ=60)
                                                                                                                                        CTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTG           <  SRR19973072.773098/150‑1 (MQ=60)
                                                                                                                                         TTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGT          >  SRR19973072.10520/1‑150 (MQ=60)
                                                                                                                                          TCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGTT         >  SRR19973072.673035/1‑150 (MQ=60)
                                                                                                                                           CGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGTTT        >  SRR19973072.2921773/1‑150 (MQ=60)
                                                                                                                                           CGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGTTT        >  SRR19973072.1106651/1‑150 (MQ=60)
                                                                                                                                           CGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGTTT        <  SRR19973072.2945668/150‑1 (MQ=60)
                                                                                                                                             CTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGTTTTA      >  SRR19973072.3187028/1‑150 (MQ=60)
                                                                                                                                             CTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGTTTTA      <  SRR19973072.3116446/150‑1 (MQ=60)
                                                                                                                                              TGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGTTTTAC     >  SRR19973072.2662212/1‑150 (MQ=60)
                                                                                                                                              TGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGTTTTAC     >  SRR19973072.2962654/1‑150 (MQ=60)
                                                                                                                                               GACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGTTTTACG    <  SRR19973072.1616607/150‑1 (MQ=60)
                                                                                                                                               GACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGTTTTACG    <  SRR19973072.1616550/150‑1 (MQ=60)
                                                                                                                                                ACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGTTTTACGT   >  SRR19973072.1247148/1‑150 (MQ=60)
                                                                                                                                                 CATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGTTTTACGTT  <  SRR19973072.451832/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                  
TTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGTTTTACGTT  >  CP009273/261966‑262260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑