Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 262,114 C→A 100% D51Y (GAT→TAT)  yafY ← lipoprotein, inner membrane; overproduction stimulates degP expression; CP4‑6 prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273262,1140CA97.9% 640.4 / 3.2 205D51Y (GAT→TAT) yafYlipoprotein, inner membrane; overproduction stimulates degP expression; CP4‑6 prophage
Reads supporting (aligned to +/- strand):  ref base C (4/1);  new base A (81/119);  total (85/120)
Fisher's exact test for biased strand distribution p-value = 1.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 209 total aligned reads displayed.

TCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGTTTTA  >  CP009273/261970‑262256
                                                                                                                                                |                                                                                                                                              
tcaGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACataatc                                                                                                                                             <  1:2437191/148‑1 (MQ=255)
tcaGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACataatc                                                                                                                                             >  2:1166874/1‑148 (MQ=255)
tcaGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACataatc                                                                                                                                             <  2:1067474/148‑1 (MQ=255)
 caGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATcc                                                                                                                                            <  1:378676/148‑1 (MQ=255)
  aGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCt                                                                                                                                           >  1:2204380/1‑148 (MQ=255)
  aGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCt                                                                                                                                           <  1:201846/148‑1 (MQ=255)
   gTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCtg                                                                                                                                          <  1:136025/148‑1 (MQ=255)
   gTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCtg                                                                                                                                          <  1:148494/148‑1 (MQ=255)
   gTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCtg                                                                                                                                          >  1:1900719/1‑148 (MQ=255)
      gCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCtggtg                                                                                                                                       >  2:2645644/1‑148 (MQ=255)
      gCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCtggtg                                                                                                                                       >  2:2434441/1‑148 (MQ=255)
        tGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGc                                                                                                                                     <  1:3207332/148‑1 (MQ=255)
        tGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGc                                                                                                                                     <  2:2881607/148‑1 (MQ=255)
          gACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTg                                                                                                                                   >  2:1420653/1‑148 (MQ=255)
          gACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTg                                                                                                                                   >  2:3240347/1‑148 (MQ=255)
           aCGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGa                                                                                                                                  >  2:2859718/1‑148 (MQ=255)
             ggTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGAcc                                                                                                                                >  1:3160248/1‑148 (MQ=255)
              gTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACca                                                                                                                               >  2:1495634/1‑148 (MQ=255)
                  tttCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATc                                                                                                                           <  1:2872412/148‑1 (MQ=255)
                   ttCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCa                                                                                                                          <  1:2041410/148‑1 (MQ=255)
                   ttCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCa                                                                                                                          <  1:269420/148‑1 (MQ=255)
                   ttCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCa                                                                                                                          <  1:1156958/148‑1 (MQ=255)
                   ttCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCa                                                                                                                          >  1:1102415/1‑148 (MQ=255)
                      cTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTg                                                                                                                       <  2:3121815/148‑1 (MQ=255)
                       tGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGa                                                                                                                      >  2:3177971/1‑148 (MQ=255)
                        gCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGaa                                                                                                                     <  2:2090075/148‑1 (MQ=255)
                         cATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGaaa                                                                                                                    <  2:1818347/148‑1 (MQ=255)
                            atCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAAcgc                                                                                                                 >  1:2844341/1‑148 (MQ=255)
                            atCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAAcgc                                                                                                                 >  2:777031/1‑148 (MQ=255)
                              ccACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCgg                                                                                                               <  2:2279253/148‑1 (MQ=255)
                                aCGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTg                                                                                                             <  1:3240735/148‑1 (MQ=255)
                                    ggTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGt                                                                                                         >  2:3096091/1‑148 (MQ=255)
                                      tagtagtaACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGa                                                                                                       >  2:872748/1‑148 (MQ=255)
                                         tagtaACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGt                                                                                                    <  1:2303462/148‑1 (MQ=255)
                                         tagtaACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGt                                                                                                    >  1:1350401/1‑148 (MQ=255)
                                         tagtaACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGt                                                                                                    >  1:3320976/1‑148 (MQ=255)
                                          agtaACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTc                                                                                                   <  1:31951/148‑1 (MQ=255)
                                          agtaACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCAGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTc                                                                                                   <  2:1698714/148‑1 (MQ=255)
                                               cTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTaa                                                                                              >  2:1565020/1‑148 (MQ=255)
                                               cTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTaa                                                                                              >  1:2181445/1‑148 (MQ=255)
                                                      ccTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACt                                                                                       >  2:1654929/1‑148 (MQ=255)
                                                      ccTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACt                                                                                       <  1:1662046/148‑1 (MQ=255)
                                                       cTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTg                                                                                      <  1:1895283/148‑1 (MQ=255)
                                                        tCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGa                                                                                     >  2:2703293/1‑148 (MQ=255)
                                                            aGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGc                                                                                 <  1:382616/148‑1 (MQ=255)
                                                            aGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGc                                                                                 <  1:2625603/148‑1 (MQ=255)
                                                             gCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGcc                                                                                >  2:2186355/1‑148 (MQ=255)
                                                             gCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGcc                                                                                >  1:1581801/1‑148 (MQ=255)
                                                               cATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCtt                                                                              >  2:484951/1‑148 (MQ=255)
                                                               cATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCtt                                                                              >  2:933797/1‑148 (MQ=255)
                                                                aTCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTc                                                                             <  2:2685245/148‑1 (MQ=255)
                                                                 tCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCa                                                                            >  1:3015802/1‑148 (MQ=255)
                                                                  cGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAg                                                                           >  1:1032980/1‑148 (MQ=255)
                                                                    ggCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGt                                                                         >  2:564331/1‑148 (MQ=255)
                                                                    ggCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGt                                                                         <  2:1289108/148‑1 (MQ=255)
                                                                       gACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGtcat                                                                      <  2:588644/148‑1 (MQ=255)
                                                                         cTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGtcatca                                                                    >  2:1522319/1‑148 (MQ=255)
                                                                            gCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACtt                                                                 <  1:154040/148‑1 (MQ=255)
                                                                            gCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACtt                                                                 <  1:3057989/148‑1 (MQ=255)
                                                                                       aaaCCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCa                                                      <  1:836797/148‑1 (MQ=255)
                                                                                       aaaCCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCa                                                      <  1:174687/148‑1 (MQ=255)
                                                                                         aCCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATg                                                    <  2:3320976/148‑1 (MQ=255)
                                                                                           cTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCTCATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCg                                                  <  2:1553348/148‑1 (MQ=255)
                                                                                           cTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCg                                                  <  1:745445/148‑1 (MQ=255)
                                                                                                aGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATgag                                             <  2:346711/148‑1 (MQ=255)
                                                                                                aGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATgag                                             >  1:1734593/1‑148 (MQ=255)
                                                                                                aGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATgag                                             <  2:345172/148‑1 (MQ=255)
                                                                                                aGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATgag                                             <  2:2218072/148‑1 (MQ=255)
                                                                                                aGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATgag                                             <  2:278514/148‑1 (MQ=255)
                                                                                                 ggTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATgaga                                            >  1:769509/1‑148 (MQ=255)
                                                                                                    gggACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAAc                                         <  2:1302047/148‑1 (MQ=255)
                                                                                                    gggACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAAc                                         <  1:2917080/148‑1 (MQ=255)
                                                                                                    gggACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAAc                                         <  1:1026173/148‑1 (MQ=255)
                                                                                                     ggACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACa                                        >  1:1995867/1‑148 (MQ=255)
                                                                                                          ggATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAgtg                                   <  2:1900719/148‑1 (MQ=255)
                                                                                                          ggATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAgtg                                   <  1:2709976/148‑1 (MQ=255)
                                                                                                           gATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAgtgg                                  <  2:843959/148‑1 (MQ=255)
                                                                                                            aTAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAgtggt                                 >  2:3351883/1‑148 (MQ=255)
                                                                                                              aaGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGtggtgg                               >  1:688828/1‑148 (MQ=255)
                                                                                                               agtagtCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGc                              >  1:2424928/1‑148 (MQ=255)
                                                                                                                 tagtCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAATTTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCAGGCGATGAGAAACAGAGTGGTGGcga                            >  2:1816393/1‑146 (MQ=255)
                                                                                                                  agtCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAgtggt                                 >  1:3097298/1‑142 (MQ=255)
                                                                                                                   gtCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAAcc                          <  2:40720/148‑1 (MQ=255)
                                                                                                                    tCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCa                         >  1:2911725/1‑148 (MQ=255)
                                                                                                                     cAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAg                        >  2:61196/1‑148 (MQ=255)
                                                                                                                        ccTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAgcgc                     <  1:638005/148‑1 (MQ=255)
                                                                                                                        ccTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAgcgc                     <  2:719782/148‑1 (MQ=255)
                                                                                                                              aCCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCcagcag               <  2:3015802/148‑1 (MQ=255)
                                                                                                                              aCCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCcagcag               <  1:3267769/148‑1 (MQ=255)
                                                                                                                               ccATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCaga              >  1:1846660/1‑148 (MQ=255)
                                                                                                                                 aTTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCACCAGAgg            >  1:43091/1‑148 (MQ=255)
                                                                                                                                    cttcGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAg         >  2:3094672/1‑148 (MQ=255)
                                                                                                                                     ttcGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAgt        <  1:2050316/148‑1 (MQ=255)
                                                                                                                                     ttcGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAgt        <  1:3081974/148‑1 (MQ=255)
                                                                                                                                     ttcGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAgt        <  2:967409/148‑1 (MQ=255)
                                                                                                                                      tcGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAgtg       <  2:182647/148‑1 (MQ=255)
                                                                                                                                      tcGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAgtg       <  2:111402/148‑1 (MQ=255)
                                                                                                                                        gCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGtt     <  1:1420653/148‑1 (MQ=255)
                                                                                                                                         cTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGttt    <  1:3501401/148‑1 (MQ=255)
                                                                                                                                         cTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGttt    <  2:213460/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                              
TCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGTTTTA  >  CP009273/261970‑262256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGTTTTACGTTTCA  >  CP009273/261967‑262263
                                                                                                                                                   |                                                                                                                                                     
TCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAAT                                                                                                                                                     <  SRR19973065.2008416/150‑1 (MQ=60)
  ATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCC                                                                                                                                                   >  SRR19973065.355740/1‑150 (MQ=60)
   TCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCT                                                                                                                                                  >  SRR19973065.2218485/1‑150 (MQ=60)
   TCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCT                                                                                                                                                  >  SRR19973065.2218462/1‑150 (MQ=60)
   TCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCT                                                                                                                                                  <  SRR19973065.2452560/150‑1 (MQ=60)
    CAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTG                                                                                                                                                 <  SRR19973065.381320/150‑1 (MQ=60)
    CAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTG                                                                                                                                                 <  SRR19973065.381534/150‑1 (MQ=60)
    CAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTG                                                                                                                                                 >  SRR19973065.1913205/1‑150 (MQ=60)
     AGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGG                                                                                                                                                >  SRR19973065.1912638/1‑150 (MQ=60)
     AGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGG                                                                                                                                                <  SRR19973065.203403/150‑1 (MQ=60)
      GTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGT                                                                                                                                               <  SRR19973065.149749/150‑1 (MQ=60)
      GTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGT                                                                                                                                               <  SRR19973065.137190/150‑1 (MQ=60)
       TGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCAGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTG                                                                                                                                              >  SRR19973065.1710042/1‑150 (MQ=60)
           TGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTG                                                                                                                                          <  SRR19973065.3225988/150‑1 (MQ=60)
           TGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTG                                                                                                                                          <  SRR19973065.3226638/150‑1 (MQ=60)
           TGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTG                                                                                                                                          >  SRR19973065.1372957/1‑150 (MQ=60)
            GGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGA                                                                                                                                         >  SRR19973065.1439604/1‑150 (MQ=60)
              ACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACC                                                                                                                                       >  SRR19973065.3179395/1‑150 (MQ=60)
                 GTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACA                                                                                                                                    >  SRR19973065.3163737/1‑150 (MQ=60)
                    TTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCA                                                                                                                                 >  SRR19973065.1109655/1‑150 (MQ=60)
                     TTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCAC                                                                                                                                <  SRR19973065.2890014/150‑1 (MQ=60)
                      TTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACT                                                                                                                               <  SRR19973065.1165412/150‑1 (MQ=60)
                      TTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACT                                                                                                                               <  SRR19973065.271584/150‑1 (MQ=60)
                      TTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACT                                                                                                                               <  SRR19973065.1163550/150‑1 (MQ=60)
                      TTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACT                                                                                                                               <  SRR19973065.1164383/150‑1 (MQ=60)
                      TTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACT                                                                                                                               <  SRR19973065.271245/150‑1 (MQ=60)
                      TTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACT                                                                                                                               <  SRR19973065.2054478/150‑1 (MQ=60)
                             ATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGC                                                                                                                        >  SRR19973065.2861845/1‑150 (MQ=60)
                             ATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCATGGTGGCTGACCACATCACTGAAACGC                                                                                                                        >  SRR19973065.2861471/1‑150 (MQ=60)
                              TATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCG                                                                                                                       <  SRR19973065.205987/150‑1 (MQ=60)
                                   ACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGG                                                                                                                  <  SRR19973065.3260264/150‑1 (MQ=60)
                                    CGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGG                                                                                                                 >  SRR19973065.2222540/1‑150 (MQ=60)
                                      AGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTG                                                                                                               >  SRR19973065.41216/1‑150 (MQ=60)
                                       GGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGA                                                                                                              <  SRR19973065.1567111/150‑1 (MQ=60)
                                       GGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGA                                                                                                              >  SRR19973065.3241795/1‑150 (MQ=60)
                                          AGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGT                                                                                                           >  SRR19973065.3341235/1‑150 (MQ=60)
                                          AGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGT                                                                                                           >  SRR19973065.1359334/1‑150 (MQ=60)
                                           GTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTC                                                                                                          <  SRR19973065.3328458/150‑1 (MQ=60)
                                            TAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCC                                                                                                         <  SRR19973065.2317949/150‑1 (MQ=60)
                                            TAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCC                                                                                                         <  SRR19973065.2115147/150‑1 (MQ=60)
                                             AGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCT                                                                                                        <  SRR19973065.32350/150‑1 (MQ=60)
                                              GTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTT                                                                                                       <  SRR19973065.2626701/150‑1 (MQ=60)
                                                AACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAA                                                                                                     >  SRR19973065.3053797/1‑150 (MQ=60)
                                                AACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAA                                                                                                     >  SRR19973065.2195438/1‑150 (MQ=60)
                                                 ACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAA                                                                                                    >  SRR19973065.648308/1‑150 (MQ=60)
                                                         CCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGA                                                                                            <  SRR19973065.1673175/150‑1 (MQ=60)
                                                         CCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGA                                                                                            <  SRR19973065.1673177/150‑1 (MQ=60)
                                                          CTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGAT                                                                                           <  SRR19973065.1907737/150‑1 (MQ=60)
                                                          CTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGAT                                                                                           <  SRR19973065.1907026/150‑1 (MQ=60)
                                                           TCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATG                                                                                          <  SRR19973065.2872027/150‑1 (MQ=60)
                                                           TCGCAGCCATCGGGAGTCTGTCTGGTTCGTAAAACATGCAAGGTGTGACCGGATAAGTAGTCAAACTGTGACCATTGTTCGCTGACATAATCCTTGTGGGTGACCACATCACTGAAACGCGGTTGGGTGAGGTCCTTAAATTTACTGATG                                                                                          <  SRR19973065.2698649/150‑1 (MQ=60)
                                                             GCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGC                                                                                        <  SRR19973065.2042951/150‑1 (MQ=60)
                                                              CAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCC                                                                                       >  SRR19973065.1592349/1‑150 (MQ=60)
                                                               AGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCT                                                                                      <  SRR19973065.385291/150‑1 (MQ=60)
                                                               AGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCT                                                                                      <  SRR19973065.2641845/150‑1 (MQ=60)
                                                               AGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCT                                                                                      <  SRR19973065.1368900/150‑1 (MQ=60)
                                                                 CCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTC                                                                                    <  SRR19973065.3086662/150‑1 (MQ=60)
                                                                  CATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCA                                                                                   >  SRR19973065.1777089/1‑150 (MQ=60)
                                                                  CATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCA                                                                                   >  SRR19973065.3034273/1‑150 (MQ=60)
                                                                   ATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAG                                                                                  >  SRR19973065.1039815/1‑150 (MQ=60)
                                                                      GGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTC                                                                               <  SRR19973065.1943899/150‑1 (MQ=60)
                                                                      GGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTC                                                                               >  SRR19973065.2451559/1‑150 (MQ=60)
                                                                               GCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCT                                                                      <  SRR19973065.155325/150‑1 (MQ=60)
                                                                               GCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCT                                                                      <  SRR19973065.3076682/150‑1 (MQ=60)
                                                                               GCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCT                                                                      <  SRR19973065.155263/150‑1 (MQ=60)
                                                                                 GGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGT                                                                    <  SRR19973065.722861/150‑1 (MQ=60)
                                                                                       GTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGC                                                              >  SRR19973065.1036203/1‑150 (MQ=60)
                                                                                         AAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCAT                                                            >  SRR19973065.3278473/1‑150 (MQ=60)
                                                                                         AAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCAT                                                            >  SRR19973065.1747534/1‑150 (MQ=60)
                                                                                         AAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCAT                                                            >  SRR19973065.1747536/1‑150 (MQ=60)
                                                                                          AAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATG                                                           <  SRR19973065.842250/150‑1 (MQ=60)
                                                                                          AAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATG                                                           <  SRR19973065.176079/150‑1 (MQ=60)
                                                                                           AACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGC                                                          >  SRR19973065.898441/1‑150 (MQ=60)
                                                                                           AACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGC                                                          <  SRR19973065.1065481/150‑1 (MQ=60)
                                                                                              CTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGAT                                                       <  SRR19973065.750433/150‑1 (MQ=60)
                                                                                               TGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATG                                                      <  SRR19973065.3359241/150‑1 (MQ=60)
                                                                                                 CAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAG                                                    >  SRR19973065.1746073/1‑150 (MQ=60)
                                                                                                  AAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGA                                                   >  SRR19973065.774624/1‑150 (MQ=60)
                                                                                                    GGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAA                                                 <  SRR19973065.2344355/150‑1 (MQ=60)
                                                                                                      TGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACA                                               >  SRR19973065.2008799/1‑150 (MQ=60)
                                                                                                       GGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAG                                              <  SRR19973065.2934823/150‑1 (MQ=60)
                                                                                                       GGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAG                                              <  SRR19973065.1032960/150‑1 (MQ=60)
                                                                                                       GGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAG                                              <  SRR19973065.2934774/150‑1 (MQ=60)
                                                                                                         GACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAG                                            <  SRR19973065.405177/150‑1 (MQ=60)
                                                                                                           CCTGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTG                                          <  SRR19973065.2958457/150‑1 (MQ=60)
                                                                                                             GGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGT                                        <  SRR19973065.2726826/150‑1 (MQ=60)
                                                                                                               ATAAGTAGTCAACCTGTGTCCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGG                                      <  SRR19973065.620615/150‑1 (MQ=60)
                                                                                                               ATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGG                                      <  SRR19973065.3197177/150‑1 (MQ=60)
                                                                                                               ATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGG                                      >  SRR19973065.693407/1‑150 (MQ=60)
                                                                                                                TAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGC                                     >  SRR19973065.2440225/1‑150 (MQ=60)
                                                                                                                 AAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCA                                    <  SRR19973065.3078034/150‑1 (MQ=60)
                                                                                                                   GTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGagat                                  >  SRR19973065.3116213/1‑146 (MQ=60)
                                                                                                                     AGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCA                                >  SRR19973065.2929445/1‑150 (MQ=60)
                                                                                                                     AGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCA                                >  SRR19973065.2929424/1‑150 (MQ=60)
                                                                                                                           CCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCA                          <  SRR19973065.2396914/150‑1 (MQ=60)
                                                                                                                           CCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCA                          <  SRR19973065.642184/150‑1 (MQ=60)
                                                                                                                               TGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAG                      <  SRR19973065.2009777/150‑1 (MQ=60)
                                                                                                                               TGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAG                      >  SRR19973065.752732/1‑150 (MQ=60)
                                                                                                                                GACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGA                     >  SRR19973065.1858827/1‑150 (MQ=60)
                                                                                                                                 ACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAG                    <  SRR19973065.3287584/150‑1 (MQ=60)
                                                                                                                                  CCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCACCAGAGG                   >  SRR19973065.43608/1‑150 (MQ=60)
                                                                                                                                        TTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGT             <  SRR19973065.3100797/150‑1 (MQ=60)
                                                                                                                                        TTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGT             <  SRR19973065.3100794/150‑1 (MQ=60)
                                                                                                                                        TTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGT             <  SRR19973065.2063415/150‑1 (MQ=60)
                                                                                                                                         TCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGTT            <  SRR19973065.782197/150‑1 (MQ=60)
                                                                                                                                           GCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGTTTT          <  SRR19973065.1429924/150‑1 (MQ=60)
                                                                                                                                            CTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGTTTTA         <  SRR19973065.3522663/150‑1 (MQ=60)
                                                                                                                                                   AATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGATGTCCTTAAATTTACTGATGTACTTCAGGTCATCACTTATGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGTTTTACGTTTCT  >  SRR19973065.1881786/1‑150 (MQ=60)
                                                                                                                                                   AATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGTTTTACGTTTCA  >  SRR19973065.3449809/1‑150 (MQ=60)
                                                                                                                                                   AATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTAACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGGTTTACGTTTCA  >  SRR19973065.1946603/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
TCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGTTTTACGTTTCA  >  CP009273/261967‑262263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑