Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 735,817 G→T 7.3% L285L (CTG→CTT)  phr → deoxyribodipyrimidine photolyase, FAD‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273735,8170GT 7.3% 1050.0 / 35.3 274L285L (CTG→CTT) phrdeoxyribodipyrimidine photolyase, FAD‑binding
Reads supporting (aligned to +/- strand):  ref base G (99/155);  new base T (8/12);  total (107/167)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 277 total aligned reads displayed.

GCCGTTTGTCGGCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGCTATGCGTCAGCTTAA  >  CP009273/735669‑735966
                                                                                                                                                    |                                                                                                                                                     
  cGTTTGTCGGCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATa                                                                                                                                                    <  2:2293550/150‑1 (MQ=255)
    tttGTCGGCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAAc                                                                                                                                                  <  1:2295163/150‑1 (MQ=255)
      tGTCGGCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGt                                                                                                                                                <  2:339381/150‑1 (MQ=255)
       gTCGGCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTa                                                                                                                                               <  2:1793709/150‑1 (MQ=255)
        tCGGCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTAt                                                                                                                                              <  2:1135474/150‑1 (MQ=255)
          ggCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCa                                                                                                                                            >  2:718232/1‑150 (MQ=255)
          ggCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCa                                                                                                                                            >  2:544324/1‑150 (MQ=255)
          ggCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCa                                                                                                                                            >  2:4075045/1‑150 (MQ=255)
           gCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCAc                                                                                                                                           >  1:2683017/1‑150 (MQ=255)
             cAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCAccc                                                                                                                                         >  1:4438692/1‑150 (MQ=255)
             cAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCAccc                                                                                                                                         >  1:3469499/1‑150 (MQ=255)
                ccTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTc                                                                                                                                      <  1:3906279/150‑1 (MQ=255)
                ccTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTc                                                                                                                                      <  2:1841542/150‑1 (MQ=255)
                 cTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCg                                                                                                                                     >  2:1615930/1‑150 (MQ=255)
                 cTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCg                                                                                                                                     <  1:2104344/150‑1 (MQ=255)
                 cTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCg                                                                                                                                     <  2:2453057/150‑1 (MQ=255)
                  tGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTTATAACGTATCACCCCTCGt                                                                                                                                    >  1:4591645/1‑150 (MQ=255)
                   ggCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTTATAACGTATCACCCCTCGtt                                                                                                                                   <  2:4051914/150‑1 (MQ=255)
                           gcgggTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAAc                                                                                                                           <  1:4416882/150‑1 (MQ=255)
                            cgggTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACa                                                                                                                          >  1:1787203/1‑150 (MQ=255)
                                ttATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGt                                                                                                                      <  1:2624020/150‑1 (MQ=255)
                                 tATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTc                                                                                                                     >  2:2238875/1‑150 (MQ=255)
                                  aTCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTcc                                                                                                                    >  2:164960/1‑150 (MQ=255)
                                   tcgcctcgccAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCa                                                                                                                   <  1:119340/150‑1 (MQ=255)
                                   tcgcctcgccAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCa                                                                                                                   >  2:4524407/1‑150 (MQ=255)
                                   tcgcctcgccAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCa                                                                                                                   >  2:3165797/1‑150 (MQ=255)
                                   tcgcctcgccAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCa                                                                                                                   >  2:2787891/1‑150 (MQ=255)
                                     gactcgccAGTGCTTGCATGGCTTGTTGGCTGAACTGCCGCTGTCTCTGGACGGTGGTGCCTGTAGTGTCTGGCTTAATGTTCTGTTCTGGCGCGTGTTTTACCGTCACCTGATAACGTATTACCCCTCGTTGTGTTAACTTCGTCCAtt                                                                                                                 <  2:2108398/148‑1 (MQ=255)
                                      cctcgccAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTTATAACGTATCACCCCTCGTTGTGTAAACATCGTCCAttt                                                                                                                <  2:1997755/150‑1 (MQ=255)
                                      cctcgccAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTTATAACGTATCACCCCTCGTTGTGTAAACATCGTCCAttt                                                                                                                <  1:2436831/150‑1 (MQ=255)
                                       ctcgccAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTGGTGTAAAAATCGTCCATTTa                                                                                                               >  2:4409181/1‑150 (MQ=255)
                                        tcgccAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTAt                                                                                                              <  2:1369519/150‑1 (MQ=255)
                                         cgccAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTAtt                                                                                                             <  2:2714854/150‑1 (MQ=255)
                                         cgccAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTAtt                                                                                                             <  2:3409385/150‑1 (MQ=255)
                                         cgccAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTAtt                                                                                                             >  1:1319797/1‑150 (MQ=255)
                                            cAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGcc                                                                                                          <  2:2182676/150‑1 (MQ=255)
                                              gTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTg                                                                                                        <  1:3942586/150‑1 (MQ=255)
                                                gCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTTATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGa                                                                                                      <  2:2554501/150‑1 (MQ=255)
                                                  ttGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTTATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACg                                                                                                    <  1:3894677/150‑1 (MQ=255)
                                                     cATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGTCGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGAt                                                                                                 <  2:940718/150‑1 (MQ=255)
                                                     cATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTTATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGAt                                                                                                 >  2:4495958/1‑150 (MQ=255)
                                                         gCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCgtg                                                                                             <  1:1661940/150‑1 (MQ=255)
                                                         gCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCgtg                                                                                             <  2:4280469/150‑1 (MQ=255)
                                                         gCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCgtg                                                                                             >  2:1776678/1‑150 (MQ=255)
                                                          cTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCgtgt                                                                                            <  1:2229294/150‑1 (MQ=255)
                                                           ttgttgGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTa                                                                                           <  2:2962563/150‑1 (MQ=255)
                                                                   tGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCa                                                                                   <  1:2774048/150‑1 (MQ=255)
                                                                    gAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCag                                                                                  >  1:1155385/1‑150 (MQ=255)
                                                                    gAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCag                                                                                  <  2:3177864/150‑1 (MQ=255)
                                                                          ccGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAAt                                                                            <  1:946087/150‑1 (MQ=255)
                                                                           cGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATc                                                                           <  2:3891887/150‑1 (MQ=255)
                                                                                    tGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACAtt                                                                  >  2:3672987/1‑150 (MQ=255)
                                                                                    tGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACAtt                                                                  <  1:3756321/150‑1 (MQ=255)
                                                                                             gggCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCt                                                         <  2:2755017/150‑1 (MQ=255)
                                                                                             gggCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCt                                                         <  2:582950/150‑1 (MQ=255)
                                                                                             gggCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCt                                                         <  2:2397381/150‑1 (MQ=255)
                                                                                              ggCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTg                                                        >  1:544594/1‑150 (MQ=255)
                                                                                                ccGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGc                                                      >  1:4493300/1‑150 (MQ=255)
                                                                                                ccGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGc                                                      <  1:4084842/150‑1 (MQ=255)
                                                                                                 cGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCa                                                     <  1:3446991/150‑1 (MQ=255)
                                                                                                     aGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGaa                                                 <  1:2949377/150‑1 (MQ=255)
                                                                                                       tgtCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAgg                                               >  2:823478/1‑150 (MQ=255)
                                                                                                         tCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCa                                             <  1:2102990/150‑1 (MQ=255)
                                                                                                           tGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCaaa                                           >  2:371418/1‑150 (MQ=255)
                                                                                                           tGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCaaa                                           >  1:4176097/1‑150 (MQ=255)
                                                                                                            ggCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGACGGCaaaa                                          >  2:145758/1‑150 (MQ=255)
                                                                                                            ggCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCaaaa                                          <  1:1478105/150‑1 (MQ=255)
                                                                                                             gCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTTATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAAc                                         >  1:3652922/1‑150 (MQ=255)
                                                                                                                tAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACggg                                      <  2:300115/150‑1 (MQ=255)
                                                                                                                 aaTGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGa                                     <  2:537331/150‑1 (MQ=255)
                                                                                                                   tGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATa                                   <  1:2838420/150‑1 (MQ=255)
                                                                                                                    gAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATAc                                  <  1:2067163/150‑1 (MQ=255)
                                                                                                                     aGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATAcc                                 <  1:772950/150‑1 (MQ=255)
                                                                                                                     aGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATAcc                                 >  2:1708252/1‑150 (MQ=255)
                                                                                                                      gCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATAccc                                >  2:3995891/1‑150 (MQ=255)
                                                                                                                        tGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGa                              >  1:3263886/1‑150 (MQ=255)
                                                                                                                        tGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGa                              >  1:1733512/1‑150 (MQ=255)
                                                                                                                         gATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGAt                             <  2:1407412/150‑1 (MQ=255)
                                                                                                                          aTCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGAtt                            <  1:2372688/150‑1 (MQ=255)
                                                                                                                          aTCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGAtt                            <  1:2337418/150‑1 (MQ=255)
                                                                                                                           tCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGAttg                           <  1:1978203/150‑1 (MQ=255)
                                                                                                                             tGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGAttgtt                         >  2:3964981/1‑150 (MQ=255)
                                                                                                                                 gcgAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATg                     >  2:3879988/1‑150 (MQ=255)
                                                                                                                                   gAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGcc                   >  2:2934541/1‑150 (MQ=255)
                                                                                                                                   gAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGcc                   >  1:87514/1‑150 (MQ=255)
                                                                                                                                     gTTTTACCGTCACCTTATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGc                 >  1:40169/1‑150 (MQ=255)
                                                                                                                                     gTTTTACCGTCACCTTATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGc                 >  2:1048464/1‑150 (MQ=255)
                                                                                                                                     gTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGc                 >  2:2663890/1‑150 (MQ=255)
                                                                                                                                      ttttACCGTCACCTTATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGCt                <  1:1864961/150‑1 (MQ=255)
                                                                                                                                       tttACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGCTa               <  1:2907103/150‑1 (MQ=255)
                                                                                                                                       tttACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGCTa               <  2:607305/150‑1 (MQ=255)
                                                                                                                                         tACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGCTATg             >  1:1944973/1‑150 (MQ=255)
                                                                                                                                          aCCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGCTATGc            >  1:3195282/1‑150 (MQ=255)
                                                                                                                                              tCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGCTATGCGTCa        <  1:1951818/150‑1 (MQ=255)
                                                                                                                                              tCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGCTATGCGTCa        <  1:4366332/150‑1 (MQ=255)
                                                                                                                                               cACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTCCAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGCTATGCGTCAg       >  2:1658125/1‑150 (MQ=255)
                                                                                                                                               cACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGCTATGCGTCAg       >  2:4566656/1‑150 (MQ=255)
                                                                                                                                                  cTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGCTATGCGTCAGCtt    <  2:2625088/150‑1 (MQ=255)
                                                                                                                                                    gATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGCTATGCGTCAGCTTaa  >  1:2044935/1‑150 (MQ=255)
                                                                                                                                                    gATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGCTATGCGTCAGCTTaa  >  2:2843356/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GCCGTTTGTCGGCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGCTATGCGTCAGCTTAA  >  CP009273/735669‑735966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A