Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 735,817 G→T 5.8% L285L (CTG→CTT)  phr → deoxyribodipyrimidine photolyase, FAD‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273735,8170GT 5.8% 660.8 / 12.8 172L285L (CTG→CTT) phrdeoxyribodipyrimidine photolyase, FAD‑binding
Reads supporting (aligned to +/- strand):  ref base G (68/94);  new base T (4/6);  total (72/100)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 174 total aligned reads displayed.

CCGTTTGTCGGCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGCTATGCGTCAGCTT  >  CP009273/735670‑735964
                                                                                                                                                   |                                                                                                                                                   
ccGTTTGTCGGCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTg                                                                                                                                                     >  1:1370460/1‑148 (MQ=255)
  gTTTGTCGGCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCAGCTGAt                                                                                                                                                   >  1:1810467/1‑148 (MQ=255)
   tttGTCGGCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATa                                                                                                                                                  >  2:1192138/1‑148 (MQ=255)
    ttGTCGGCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTTATaa                                                                                                                                                 <  2:833928/148‑1 (MQ=255)
          gCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATc                                                                                                                                           <  2:2407478/148‑1 (MQ=255)
           ccAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCa                                                                                                                                          <  1:2001312/148‑1 (MQ=255)
              gCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCAccc                                                                                                                                       >  2:463652/1‑148 (MQ=255)
              gCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCAccc                                                                                                                                       >  1:847897/1‑148 (MQ=255)
               ccTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCAcccc                                                                                                                                      <  2:1459135/148‑1 (MQ=255)
                cTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCt                                                                                                                                     <  1:273126/148‑1 (MQ=255)
                cTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCt                                                                                                                                     <  2:1042881/148‑1 (MQ=255)
                 tGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTc                                                                                                                                    >  2:2209796/1‑148 (MQ=255)
                   gCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGt                                                                                                                                  >  1:1200420/1‑148 (MQ=255)
                            gggTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAAc                                                                                                                         <  1:1948667/148‑1 (MQ=255)
                                  tcgcctcgccAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTc                                                                                                                   >  1:1555037/1‑148 (MQ=255)
                                    gcctcgccAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCa                                                                                                                 <  1:3015967/148‑1 (MQ=255)
                                       tcgccAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCAttt                                                                                                              <  1:611717/148‑1 (MQ=255)
                                        cgccAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTa                                                                                                             >  2:610708/1‑148 (MQ=255)
                                          ccAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTAtt                                                                                                           <  1:2988826/148‑1 (MQ=255)
                                            aGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGc                                                                                                         <  1:1014362/148‑1 (MQ=255)
                                              tGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCt                                                                                                       >  2:1620446/1‑148 (MQ=255)
                                              tGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCt                                                                                                       >  1:2132542/1‑148 (MQ=255)
                                                 ttGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGa                                                                                                    >  1:1849800/1‑148 (MQ=255)
                                                  tGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGATGATCTGGCGCGAGTTTTACCGTCACCTTATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGAc                                                                                                   >  2:1460154/1‑148 (MQ=255)
                                                     aTCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGa                                                                                                <  2:517708/148‑1 (MQ=255)
                                                     aTCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGa                                                                                                <  2:2993029/148‑1 (MQ=255)
                                                      tCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGTTGGGTCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGAt                                                                                               >  1:2759356/1‑148 (MQ=255)
                                                              tgGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACa                                                                                       >  1:1765126/1‑148 (MQ=255)
                                                               ggCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAg                                                                                      <  2:1350394/148‑1 (MQ=255)
                                                               ggCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAg                                                                                      <  2:1350317/148‑1 (MQ=255)
                                                               ggCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAg                                                                                      <  2:2977001/148‑1 (MQ=255)
                                                                gCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGt                                                                                     <  2:1648795/148‑1 (MQ=255)
                                                                gCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGt                                                                                     <  2:2708827/148‑1 (MQ=255)
                                                                 cTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTg                                                                                    <  1:468499/148‑1 (MQ=255)
                                                                  tGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTgg                                                                                   >  2:771277/1‑148 (MQ=255)
                                                                   gAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGc                                                                                  >  2:2357627/1‑148 (MQ=255)
                                                                    aaCAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCa                                                                                 >  1:2140542/1‑148 (MQ=255)
                                                                    aaCAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCa                                                                                 >  1:483261/1‑148 (MQ=255)
                                                                    aaCAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAAAGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCa                                                                                 >  1:3127548/1‑148 (MQ=255)
                                                                      cAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCaga                                                                               >  2:947386/1‑148 (MQ=255)
                                                                      cAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCaga                                                                               >  2:3051088/1‑148 (MQ=255)
                                                                      cAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCaga                                                                               >  1:906214/1‑148 (MQ=255)
                                                                        gCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGc                                                                             >  1:43338/1‑148 (MQ=255)
                                                                         ccGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCa                                                                            >  1:2490650/1‑148 (MQ=255)
                                                                         ccGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCa                                                                            <  1:1192138/148‑1 (MQ=255)
                                                                           gCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTTATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAAt                                                                          <  2:2837794/148‑1 (MQ=255)
                                                                           gCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAAt                                                                          <  1:319380/148‑1 (MQ=255)
                                                                            cAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATc                                                                         >  2:2590914/1‑148 (MQ=255)
                                                                                  cTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGccc                                                                   >  1:443599/1‑146 (MQ=255)
                                                                                  cTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGcac                                                                   >  1:2108996/1‑148 (MQ=255)
                                                                                     gACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACAtt                                                                <  2:1575256/148‑1 (MQ=255)
                                                                                     gACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACAtt                                                                <  2:1573945/148‑1 (MQ=255)
                                                                                     gACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACAtt                                                                >  2:3147043/1‑148 (MQ=255)
                                                                                         gTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACa                                                            <  2:1370460/148‑1 (MQ=255)
                                                                                         gTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACa                                                            <  1:356495/148‑1 (MQ=255)
                                                                                         gTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACa                                                            <  2:814129/148‑1 (MQ=255)
                                                                                            gggCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTTATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGc                                                         >  2:2802592/1‑148 (MQ=255)
                                                                                              gCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCt                                                       <  2:2547868/148‑1 (MQ=255)
                                                                                               ccGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTg                                                      <  2:848622/148‑1 (MQ=255)
                                                                                                 ggTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGc                                                    <  2:2871783/148‑1 (MQ=255)
                                                                                                     gtgtCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTTATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGa                                                >  2:68650/1‑148 (MQ=255)
                                                                                                     gtgtCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGa                                                <  2:2592713/148‑1 (MQ=255)
                                                                                                     gtgtCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGa                                                <  2:2432635/148‑1 (MQ=255)
                                                                                                       gtCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTTATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAg                                              >  2:2200463/1‑148 (MQ=255)
                                                                                                         cTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGc                                            >  2:2963234/1‑148 (MQ=255)
                                                                                                           ggCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCaa                                          >  2:2617191/1‑148 (MQ=255)
                                                                                                           ggCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCaa                                          >  2:963179/1‑148 (MQ=255)
                                                                                                            gCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCaaa                                         <  2:1020110/148‑1 (MQ=255)
                                                                                                            gCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCaaa                                         <  2:1858745/148‑1 (MQ=255)
                                                                                                            gCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCaaa                                         >  1:1551986/1‑148 (MQ=255)
                                                                                                                 aTGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACggg                                    <  2:1473392/148‑1 (MQ=255)
                                                                                                                  tGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGa                                   <  2:1626101/148‑1 (MQ=255)
                                                                                                                     gCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTAGTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATAc                                <  2:398230/148‑1 (MQ=255)
                                                                                                                      cTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATAcc                               <  1:818681/148‑1 (MQ=255)
                                                                                                                         aTCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGa                            >  1:2607828/1‑148 (MQ=255)
                                                                                                                         aTCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGa                            >  2:293618/1‑148 (MQ=255)
                                                                                                                          tCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGAt                           >  1:86570/1‑148 (MQ=255)
                                                                                                                          tCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGAt                           <  1:1623987/148‑1 (MQ=255)
                                                                                                                             ggCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGAttgt                        >  1:2487266/1‑148 (MQ=255)
                                                                                                                               cgcgAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGAttgttg                      <  1:3148009/148‑1 (MQ=255)
                                                                                                                               cgcgAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGAttgttg                      <  1:1303281/148‑1 (MQ=255)
                                                                                                                                     ttttACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCg                <  1:2947508/148‑1 (MQ=255)
                                                                                                                                     ttttACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGAGTGTTGATGCCg                >  2:1818150/1‑148 (MQ=255)
                                                                                                                                      tttACCGTCACCTTATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGc               <  1:2442961/148‑1 (MQ=255)
                                                                                                                                      tttACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGc               >  1:2343758/1‑148 (MQ=255)
                                                                                                                                      tttACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGc               >  1:707416/1‑148 (MQ=255)
                                                                                                                                       ttACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGCt              <  2:2718979/148‑1 (MQ=255)
                                                                                                                                        tACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGCTa             <  2:1945597/148‑1 (MQ=255)
                                                                                                                                         aCCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTTGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGCTAt            <  1:637256/148‑1 (MQ=255)
                                                                                                                                         aCCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGCTAt            <  1:637348/148‑1 (MQ=255)
                                                                                                                                           cGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGCTATGc          >  1:1066389/1‑148 (MQ=255)
                                                                                                                                           cGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGCTATGc          >  1:1066330/1‑148 (MQ=255)
                                                                                                                                           cGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGCTATGc          <  1:2223079/148‑1 (MQ=255)
                                                                                                                                           cGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGCTATGc          <  1:2223271/148‑1 (MQ=255)
                                                                                                                                           cGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGCTATGc          <  1:2223335/148‑1 (MQ=255)
                                                                                                                                           cGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGCTATGc          >  1:2577340/1‑148 (MQ=255)
                                                                                                                                              cACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGCTATGCGTc       <  1:2534583/148‑1 (MQ=255)
                                                                                                                                              cACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGCTATGCGTc       >  1:2971103/1‑148 (MQ=255)
                                                                                                                                                 cTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGCTATGCGTCAGc    >  2:3294188/1‑148 (MQ=255)
                                                                                                                                                   gATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGCTATGCGTCAGCtt  >  2:413658/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CCGTTTGTCGGCCAGCCTGGCAACGGGCGGGTTATCGCCTCGCCAGTGCTTGCATCGCTTGTTGGCTGAACAGCCGCAGGCGCTGGACGGTGGGGCCGGTAGTGTCTGGCTTAATGAGCTGATCTGGCGCGAGTTTTACCGTCACCTGATAACGTATCACCCCTCGTTGTGTAAACATCGTCCATTTATTGCCTGGACGGATCGTGTACAGTGGCAGAGCAATCCCGCACATTTACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGCTATGCGTCAGCTT  >  CP009273/735670‑735964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A