Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 858,992 C→T 52.3% intergenic (‑25/‑106) ybiY ← / → fsaA putative pyruvate formate lyase activating enzyme/fructose‑6‑phosphate aldolase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273858,9920CT52.3% 6.7 / inf 243intergenic (‑25/‑106)ybiY/fsaAputative pyruvate formate lyase activating enzyme/fructose‑6‑phosphate aldolase 1
Reads supporting (aligned to +/- strand):  ref base C (57/59);  new base T (54/73);  total (111/132)
Fisher's exact test for biased strand distribution p-value = 3.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 244 total aligned reads displayed.

GCGTGCGGGCGCGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACGTTGTTGCGGTGAAGGC  >  CP009273/858844‑859141
                                                                                                                                                    |                                                                                                                                                     
  gtgcgGGCGCGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTAtt                                                                                                                                                    >  1:2092666/1‑150 (MQ=255)
  gtgcgGGCGCGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCAtt                                                                                                                                                    <  2:930022/150‑1 (MQ=255)
     cgGGCGCGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTaaa                                                                                                                                                 >  2:3720230/1‑150 (MQ=255)
          gcgGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACt                                                                                                                                            <  2:3988372/150‑1 (MQ=255)
           cgGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTTTCTATTTCGTTCAAGCATTAAAATACtt                                                                                                                                           >  1:429694/1‑150 (MQ=255)
            ggCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACttt                                                                                                                                          >  1:4514796/1‑150 (MQ=255)
             gCTTTCCGGGTTCTGACACCAGCGGTAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTc                                                                                                                                         <  1:3866193/150‑1 (MQ=255)
              cTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCg                                                                                                                                        >  2:3863200/1‑150 (MQ=255)
               tttCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAAAACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGa                                                                                                                                       >  2:3064846/1‑150 (MQ=255)
                 tCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAAt                                                                                                                                     >  2:1842690/1‑150 (MQ=255)
                  ccGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATATCGTTCAAGTATTAAAATACTTTCTAATg                                                                                                                                    >  1:4275558/1‑150 (MQ=255)
                   cGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGa                                                                                                                                   <  1:4423069/150‑1 (MQ=255)
                     ggTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGaaa                                                                                                                                 <  1:4464627/150‑1 (MQ=255)
                      gTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTGTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAg                                                                                                                                <  1:1972622/150‑1 (MQ=255)
                         cTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTa                                                                                                                             >  2:2555189/1‑150 (MQ=255)
                         cTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTa                                                                                                                             >  2:4020756/1‑150 (MQ=255)
                            acacCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGAt                                                                                                                          <  1:409854/150‑1 (MQ=255)
                            acacCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGAt                                                                                                                          >  1:1464655/1‑150 (MQ=255)
                               ccAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGa                                                                                                                       <  2:1652592/150‑1 (MQ=255)
                                 aGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGAtg                                                                                                                     >  1:2135380/1‑150 (MQ=255)
                                  gCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGAtgt                                                                                                                    <  1:3756187/150‑1 (MQ=255)
                                  gCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGAtgt                                                                                                                    <  2:1156767/150‑1 (MQ=255)
                                    ggCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGc                                                                                                                  <  2:1415353/150‑1 (MQ=255)
                                    ggCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGc                                                                                                                  <  2:1134091/150‑1 (MQ=255)
                                       aGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTc                                                                                                               <  1:3641175/150‑1 (MQ=255)
                                       aGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTc                                                                                                               >  2:4493470/1‑150 (MQ=255)
                                           cAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACt                                                                                                           >  1:4041467/1‑150 (MQ=255)
                                            aGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTg                                                                                                          >  1:60526/1‑150 (MQ=255)
                                               gAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTc                                                                                                       >  2:3170608/1‑150 (MQ=255)
                                                aaCAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGTGTCGATTAGCGCTTAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCa                                                                                                      <  2:4042488/150‑1 (MQ=255)
                                                 aCAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCag                                                                                                     <  2:170197/150‑1 (MQ=255)
                                                   aGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCagag                                                                                                   >  1:1441801/1‑150 (MQ=255)
                                                      cTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGt                                                                                                <  1:3773458/150‑1 (MQ=255)
                                                       tttAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGtt                                                                                               <  2:3309426/150‑1 (MQ=255)
                                                       tttAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGtt                                                                                               <  1:4596181/150‑1 (MQ=255)
                                                        ttAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGttt                                                                                              <  2:388526/150‑1 (MQ=255)
                                                         tAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGtttt                                                                                             <  1:2566648/150‑1 (MQ=255)
                                                          aaGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTc                                                                                            <  1:2702795/150‑1 (MQ=255)
                                                          aaGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTc                                                                                            >  2:2800388/1‑150 (MQ=255)
                                                          aaGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGGAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTc                                                                                            <  2:3717686/150‑1 (MQ=255)
                                                            gAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTccc                                                                                          <  1:133037/150‑1 (MQ=255)
                                                             aaaTACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCg                                                                                         >  1:4406525/1‑150 (MQ=255)
                                                             aaaTACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCg                                                                                         <  1:345903/150‑1 (MQ=255)
                                                                   gACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAg                                                                                   >  1:3523978/1‑150 (MQ=255)
                                                                   gACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAg                                                                                   >  2:117526/1‑150 (MQ=255)
                                                                      cGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCt                                                                                <  1:4136798/150‑1 (MQ=255)
                                                                       gTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTa                                                                               <  2:2223100/150‑1 (MQ=255)
                                                                       gTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTa                                                                               >  2:2069822/1‑150 (MQ=255)
                                                                         gCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACa                                                                             <  1:2294030/150‑1 (MQ=255)
                                                                          cGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACAt                                                                            >  2:1873537/1‑150 (MQ=255)
                                                                              tACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAg                                                                        <  1:283566/150‑1 (MQ=255)
                                                                               aCCGGGGCGATCATGGGTCGAGTAGCGCTGAATATTGAATATCATAGTTGCCTATCTATTTCGTTCAAGTATTAATATTCTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGa                                                                       <  1:2010568/150‑1 (MQ=255)
                                                                                    ggCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACaaaa                                                                  <  1:3831251/150‑1 (MQ=255)
                                                                                       catcatGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGt                                                               <  1:3026414/150‑1 (MQ=255)
                                                                                         tcatGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTAc                                                             <  1:1377742/150‑1 (MQ=255)
                                                                                         tcatGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTAc                                                             <  2:4421436/150‑1 (MQ=255)
                                                                                           atGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACAt                                                           <  1:276482/150‑1 (MQ=255)
                                                                                           atGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACAt                                                           <  2:157970/150‑1 (MQ=255)
                                                                                              ggTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACAtttt                                                        <  1:4025174/150‑1 (MQ=255)
                                                                                                  gAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAgg                                                    <  1:1066764/150‑1 (MQ=255)
                                                                                                   aGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGa                                                   >  2:2121915/1‑150 (MQ=255)
                                                                                                   aGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGa                                                   >  2:252887/1‑150 (MQ=255)
                                                                                                   aGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGa                                                   >  2:2871983/1‑150 (MQ=255)
                                                                                                    gTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGAt                                                  <  1:3276508/150‑1 (MQ=255)
                                                                                                       gcgcTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGt                                               <  1:1573985/150‑1 (MQ=255)
                                                                                                          cTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTAt                                            >  1:4066413/1‑150 (MQ=255)
                                                                                                          cTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTAt                                            >  2:1039282/1‑150 (MQ=255)
                                                                                                           tGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATg                                           <  2:2174079/150‑1 (MQ=255)
                                                                                                            gAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATgg                                          >  2:451416/1‑150 (MQ=255)
                                                                                                             aaTATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGa                                         >  1:3389675/1‑150 (MQ=255)
                                                                                                             aaTATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGa                                         <  2:3127434/150‑1 (MQ=255)
                                                                                                             aaTATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGCTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGa                                         >  1:1830311/1‑150 (MQ=255)
                                                                                                             aaTATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGCTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGa                                         >  1:4161430/1‑150 (MQ=255)
                                                                                                              atatTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGaa                                        <  1:3877672/150‑1 (MQ=255)
                                                                                                               tatTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAAc                                       >  2:432615/1‑150 (MQ=255)
                                                                                                                   gAAAATCATATTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATTAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCAGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTa                                   >  1:101175/1‑150 (MQ=255)
                                                                                                                         cATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGa                             >  1:1121439/1‑150 (MQ=255)
                                                                                                                           tAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATa                           >  2:3858220/1‑150 (MQ=255)
                                                                                                                           tAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATa                           <  2:4018973/150‑1 (MQ=255)
                                                                                                                             gTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACt                         >  2:668604/1‑150 (MQ=255)
                                                                                                                               tGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTc                       >  2:721257/1‑150 (MQ=255)
                                                                                                                               tGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTc                       >  2:2217859/1‑150 (MQ=255)
                                                                                                                               tGCCTCTCTAGTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTc                       <  2:1077762/150‑1 (MQ=255)
                                                                                                                                  ctctctATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGa                    >  1:2424068/1‑150 (MQ=255)
                                                                                                                                  ctctctATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGa                    >  2:1257119/1‑150 (MQ=255)
                                                                                                                                   tctctATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGAc                   <  2:92701/150‑1 (MQ=255)
                                                                                                                                    ctctATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACg                  >  2:2449267/1‑150 (MQ=255)
                                                                                                                                     tctATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACgt                 >  1:3924995/1‑150 (MQ=255)
                                                                                                                                     tctATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACgt                 >  1:3147933/1‑150 (MQ=255)
                                                                                                                                      ctATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACgtt                >  2:4142496/1‑150 (MQ=255)
                                                                                                                                      ctATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACgtt                >  2:3471511/1‑150 (MQ=255)
                                                                                                                                       tATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACgttg               <  2:3130629/150‑1 (MQ=255)
                                                                                                                                        aTTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACgttgt              >  2:571229/1‑150 (MQ=255)
                                                                                                                                        aTTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACgttgt              >  1:3904798/1‑150 (MQ=255)
                                                                                                                                            cGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACGTTGTTGCg          <  1:501643/150‑1 (MQ=255)
                                                                                                                                               tCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACGTTGTTGCGGTg       >  2:2074662/1‑150 (MQ=255)
                                                                                                                                               tCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACGTTGTTGCGGTg       >  2:3655547/1‑150 (MQ=255)
                                                                                                                                                  aGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACGTTGTTGCGGTGAAg    <  2:3773074/150‑1 (MQ=255)
                                                                                                                                                   gCATTAAAATAATTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGAGGGTTATGTAACTGTATCTGGATACTTCAGACGTTGTTGCGGTGAAgg   >  1:1740540/1‑150 (MQ=255)
                                                                                                                                                    cATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACGTTGTTGCGGTGAAGgc  <  1:2915498/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GCGTGCGGGCGCGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACGTTGTTGCGGTGAAGGC  >  CP009273/858844‑859141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGTGCGGGCGCGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACGTTGTTGCGGTGAAGGC  >  CP009273/858844‑859141
                                                                                                                                                    |                                                                                                                                                     
GCGTGCGGGCGCGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCA                                                                                                                                                      <  SRR19973072.3878033/150‑1 (MQ=60)
  GTGCGGGCGCGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATT                                                                                                                                                    >  SRR19973072.2097224/1‑150 (MQ=60)
     CGGGCGCGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAA                                                                                                                                                 <  SRR19973072.1361060/150‑1 (MQ=60)
         CGCGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATAC                                                                                                                                             <  SRR19973072.3645718/150‑1 (MQ=60)
          GCGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACT                                                                                                                                            <  SRR19973072.2073904/150‑1 (MQ=60)
          GCGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACT                                                                                                                                            <  SRR19973072.351888/150‑1 (MQ=60)
           CGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTTTCTATTTCGTTCAAGCATTAAAATACTT                                                                                                                                           >  SRR19973072.430681/1‑150 (MQ=60)
           CGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTTTCTATTTCGTTCAAGCATTAAAATACTT                                                                                                                                           >  SRR19973072.430663/1‑150 (MQ=60)
            GGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTT                                                                                                                                          >  SRR19973072.4524362/1‑150 (MQ=60)
            GGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTT                                                                                                                                          >  SRR19973072.4524327/1‑150 (MQ=60)
             GCTTTCCGGGTTCTGACACCAGCGGTAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTC                                                                                                                                         <  SRR19973072.3874236/150‑1 (MQ=60)
                  CCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATG                                                                                                                                    <  SRR19973072.4064952/150‑1 (MQ=60)
                  CCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATG                                                                                                                                    <  SRR19973072.4064961/150‑1 (MQ=60)
                  CCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATATCGTTCAAGTATTAAAATACTTTCTAATG                                                                                                                                    >  SRR19973072.4284455/1‑150 (MQ=60)
                   CGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGA                                                                                                                                   <  SRR19973072.4432389/150‑1 (MQ=60)
                   CGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGA                                                                                                                                   <  SRR19973072.4432376/150‑1 (MQ=60)
                   CGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGA                                                                                                                                   <  SRR19973072.2888841/150‑1 (MQ=60)
                    GGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAA                                                                                                                                  <  SRR19973072.4002153/150‑1 (MQ=60)
                     GGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAA                                                                                                                                 <  SRR19973072.4474049/150‑1 (MQ=60)
                     GGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAA                                                                                                                                 <  SRR19973072.4474067/150‑1 (MQ=60)
                      GTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAG                                                                                                                                >  SRR19973072.2192576/1‑150 (MQ=60)
                      GTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTGTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAG                                                                                                                                <  SRR19973072.1976849/150‑1 (MQ=60)
                        TCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTT                                                                                                                              >  SRR19973072.2808801/1‑150 (MQ=60)
                         CTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTA                                                                                                                             <  SRR19973072.1467105/150‑1 (MQ=60)
                            ACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGAT                                                                                                                          <  SRR19973072.410765/150‑1 (MQ=60)
                            ACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGAT                                                                                                                          >  SRR19973072.1467859/1‑150 (MQ=60)
                            ACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGAT                                                                                                                          >  SRR19973072.1467804/1‑150 (MQ=60)
                             CACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATT                                                                                                                         <  SRR19973072.1228306/150‑1 (MQ=60)
                                CAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGAT                                                                                                                      <  SRR19973072.3963306/150‑1 (MQ=60)
                                 AGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATG                                                                                                                     >  SRR19973072.2140032/1‑150 (MQ=60)
                                  GCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGT                                                                                                                    <  SRR19973072.3764081/150‑1 (MQ=60)
                                   CGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTG                                                                                                                   <  SRR19973072.44646/150‑1 (MQ=60)
                                       AGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTC                                                                                                               <  SRR19973072.3648848/150‑1 (MQ=60)
                                          CCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAAC                                                                                                            <  SRR19973072.4045325/150‑1 (MQ=60)
                                           CAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACT                                                                                                           >  SRR19973072.4049945/1‑150 (MQ=60)
                                            AGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTG                                                                                                          >  SRR19973072.60689/1‑150 (MQ=60)
                                               GAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTC                                                                                                       <  SRR19973072.1944499/150‑1 (MQ=60)
                                                   AGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAG                                                                                                   >  SRR19973072.1444909/1‑150 (MQ=60)
                                                   AGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAG                                                                                                   >  SRR19973072.2649367/1‑150 (MQ=60)
                                                    GCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGA                                                                                                  >  SRR19973072.4279951/1‑150 (MQ=60)
                                                      CTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATAAAAATACTTTAGAATGAAAGTTAGATTGATGTGCGTCAAATGTTCAGAGAGT                                                                                                >  SRR19973072.225920/1‑150 (MQ=60)
                                                      CTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCGGAGAGT                                                                                                <  SRR19973072.3781365/150‑1 (MQ=60)
                                                      CTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGT                                                                                                <  SRR19973072.3781371/150‑1 (MQ=60)
                                                      CTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGT                                                                                                <  SRR19973072.340970/150‑1 (MQ=60)
                                                       TTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTT                                                                                               <  SRR19973072.4605829/150‑1 (MQ=60)
                                                       TTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTT                                                                                               <  SRR19973072.4605853/150‑1 (MQ=60)
                                                         TAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTT                                                                                             <  SRR19973072.2572205/150‑1 (MQ=60)
                                                          AAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTC                                                                                            <  SRR19973072.2708558/150‑1 (MQ=60)
                                                          AAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTC                                                                                            <  SRR19973072.2708545/150‑1 (MQ=60)
                                                           ATAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCC                                                                                           >  SRR19973072.1035633/1‑150 (MQ=60)
                                                            GAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCC                                                                                          <  SRR19973072.2115904/150‑1 (MQ=60)
                                                            GAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCC                                                                                          <  SRR19973072.133372/150‑1 (MQ=60)
                                                            GAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCC                                                                                          <  SRR19973072.1095181/150‑1 (MQ=60)
                                                            GAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCC                                                                                          <  SRR19973072.3171050/150‑1 (MQ=60)
                                                            GAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCC                                                                                          <  SRR19973072.3171089/150‑1 (MQ=60)
                                                             AAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCG                                                                                         >  SRR19973072.4415794/1‑150 (MQ=60)
                                                             AAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCG                                                                                         >  SRR19973072.4415798/1‑150 (MQ=60)
                                                             AAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCG                                                                                         <  SRR19973072.346678/150‑1 (MQ=60)
                                                                   GACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAG                                                                                   >  SRR19973072.3531379/1‑150 (MQ=60)
                                                                   GACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAG                                                                                   <  SRR19973072.3688603/150‑1 (MQ=60)
                                                                      CGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCT                                                                                <  SRR19973072.53451/150‑1 (MQ=60)
                                                                      CGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCT                                                                                <  SRR19973072.4145479/150‑1 (MQ=60)
                                                                         GCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACA                                                                             <  SRR19973072.2298940/150‑1 (MQ=60)
                                                                         GCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACA                                                                             >  SRR19973072.3883758/1‑150 (MQ=60)
                                                                           GGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATT                                                                           <  SRR19973072.3360898/150‑1 (MQ=60)
                                                                           GGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATT                                                                           <  SRR19973072.2486990/150‑1 (MQ=60)
                                                                              TACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAG                                                                        <  SRR19973072.736211/150‑1 (MQ=60)
                                                                              TACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAG                                                                        <  SRR19973072.284230/150‑1 (MQ=60)
                                                                              TACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTACCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAG                                                                        <  SRR19973072.2935402/150‑1 (MQ=60)
                                                                               ACCGGGGCGATCATGGGTCGAGTAGCGCTGAATATTGAATATCATAGTTGCCTATCTATTTCGTTCAAGTATTAATATTCTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGA                                                                       <  SRR19973072.2014904/150‑1 (MQ=60)
                                                                               ACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGA                                                                       <  SRR19973072.2014906/150‑1 (MQ=60)
                                                                                   GGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAA                                                                   >  SRR19973072.3931164/1‑150 (MQ=60)
                                                                                    GGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAA                                                                  <  SRR19973072.3839245/150‑1 (MQ=60)
                                                                                    GGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAA                                                                  <  SRR19973072.3839258/150‑1 (MQ=60)
                                                                                    GGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAA                                                                  <  SRR19973072.3839320/150‑1 (MQ=60)
                                                                                     GCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAA                                                                 <  SRR19973072.3455138/150‑1 (MQ=60)
                                                                                       CATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGT                                                               >  SRR19973072.2303855/1‑150 (MQ=60)
                                                                                       CATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGT                                                               <  SRR19973072.3032829/150‑1 (MQ=60)
                                                                                         TCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTAC                                                             <  SRR19973072.1380744/150‑1 (MQ=60)
                                                                                           ATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACAT                                                           <  SRR19973072.277130/150‑1 (MQ=60)
                                                                                           ATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACAT                                                           >  SRR19973072.1906325/1‑150 (MQ=60)
                                                                                           ATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACAT                                                           <  SRR19973072.4138061/150‑1 (MQ=60)
                                                                                           ATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACAT                                                           <  SRR19973072.4138231/150‑1 (MQ=60)
                                                                                              GGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTT                                                        <  SRR19973072.4033633/150‑1 (MQ=60)
                                                                                                  GAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGG                                                    <  SRR19973072.1069045/150‑1 (MQ=60)
                                                                                                   AGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTGTGAGGA                                                   >  SRR19973072.308500/1‑150 (MQ=60)
                                                                                                    GTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGAT                                                  <  SRR19973072.3283461/150‑1 (MQ=60)
                                                                                                       GCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGT                                               <  SRR19973072.1591835/150‑1 (MQ=60)
                                                                                                       GCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGT                                               <  SRR19973072.1577343/150‑1 (MQ=60)
                                                                                                       GCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGT                                               <  SRR19973072.1577332/150‑1 (MQ=60)
                                                                                                          CTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTAT                                            >  SRR19973072.4074944/1‑150 (MQ=60)
                                                                                                             AATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGA                                         >  SRR19973072.3396837/1‑150 (MQ=60)
                                                                                                             AATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGCTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGA                                         >  SRR19973072.1238740/1‑150 (MQ=60)
                                                                                                             AATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGCTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGA                                         >  SRR19973072.1834294/1‑150 (MQ=60)
                                                                                                             AATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGCTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGA                                         >  SRR19973072.4170151/1‑150 (MQ=60)
                                                                                                              ATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAA                                        <  SRR19973072.3885736/150‑1 (MQ=60)
                                                                                                                ATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACT                                      >  SRR19973072.3792639/1‑150 (MQ=60)
                                                                                                                   GAAAATCATATTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATTAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCAGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTA                                   >  SRR19973072.101433/1‑150 (MQ=60)
                                                                                                                    AAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTAT                                  <  SRR19973072.4502961/150‑1 (MQ=60)
                                                                                                                        TCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGG                              <  SRR19973072.1396545/150‑1 (MQ=60)
                                                                                                                         CATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGA                             >  SRR19973072.1123802/1‑150 (MQ=60)
                                                                                                                               TGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTC                       >  SRR19973072.2460107/1‑150 (MQ=60)
                                                                                                                               TGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTC                       >  SRR19973072.141750/1‑150 (MQ=60)
                                                                                                                               TGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTC                       <  SRR19973072.4200275/150‑1 (MQ=60)
                                                                                                                               TGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTC                       <  SRR19973072.4167887/150‑1 (MQ=60)
                                                                                                                                 CCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAG                     >  SRR19973072.4319708/1‑150 (MQ=60)
                                                                                                                                  CTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGA                    >  SRR19973072.2429324/1‑150 (MQ=60)
                                                                                                                                    CTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACG                  >  SRR19973072.2954361/1‑150 (MQ=60)
                                                                                                                                     TCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACGT                 >  SRR19973072.4616434/1‑150 (MQ=60)
                                                                                                                                     TCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACGT                 >  SRR19973072.3933192/1‑150 (MQ=60)
                                                                                                                                     TCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACGT                 >  SRR19973072.3154577/1‑150 (MQ=60)
                                                                                                                                        ATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACGTTGT              >  SRR19973072.3722321/1‑150 (MQ=60)
                                                                                                                                        ATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACGTTGT              >  SRR19973072.3912935/1‑150 (MQ=60)
                                                                                                                                        ATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACGTTGT              >  SRR19973072.3912929/1‑150 (MQ=60)
                                                                                                                                           TCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACGTTGTTGC           >  SRR19973072.4103354/1‑150 (MQ=60)
                                                                                                                                            CGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACGTTGTTGCG          <  SRR19973072.502831/150‑1 (MQ=60)
                                                                                                                                            CGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACGTTGTTGCG          <  SRR19973072.1977570/150‑1 (MQ=60)
                                                                                                                                                  AGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACGTTGTTGCGGTGAAG    >  SRR19973072.3589865/1‑150 (MQ=60)
                                                                                                                                                  AGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACGTTGTTGCGGTGAAG    >  SRR19973072.3589834/1‑150 (MQ=60)
                                                                                                                                                  AGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACGTTGTTGCGGTGAAG    <  SRR19973072.2807240/150‑1 (MQ=60)
                                                                                                                                                   GCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACGTTGTTGCGGTGAAGG   >  SRR19973072.1744351/1‑150 (MQ=60)
                                                                                                                                                   GCATTAAAATAATTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGAGGGTTATGTAACTGTATCTGGATACTTCAGACGTTGTTGCGGTGAAGG   >  SRR19973072.1744348/1‑150 (MQ=60)
                                                                                                                                                    CATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACGTTGTTGCGGTGAAGGC  <  SRR19973072.2921744/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
GCGTGCGGGCGCGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACGTTGTTGCGGTGAAGGC  >  CP009273/858844‑859141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑