Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 858,992 C→T 54.6% intergenic (‑25/‑106) ybiY ← / → fsaA putative pyruvate formate lyase activating enzyme/fructose‑6‑phosphate aldolase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273858,9920CT54.6% 77.0 / inf 238intergenic (‑25/‑106)ybiY/fsaAputative pyruvate formate lyase activating enzyme/fructose‑6‑phosphate aldolase 1
Reads supporting (aligned to +/- strand):  ref base C (51/57);  new base T (49/81);  total (100/138)
Fisher's exact test for biased strand distribution p-value = 1.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 239 total aligned reads displayed.

CGTGCGGGCGCGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACGTTGTTGCGGTGAAGGC  >  CP009273/858845‑859141
                                                                                                                                                   |                                                                                                                                                     
cgtgcgGGCGCGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCAt                                                                                                                                                     <  2:1861682/150‑1 (MQ=255)
cgtgcgGGCGCGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCAt                                                                                                                                                     <  2:3439010/150‑1 (MQ=255)
cgtgcgGGCGCGGCTGTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCAt                                                                                                                                                     <  2:3438982/150‑1 (MQ=255)
 gtgcgGGCGCGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTAtt                                                                                                                                                    >  1:3048431/1‑150 (MQ=255)
  tgcgGGCGCGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTa                                                                                                                                                   >  1:2891214/1‑150 (MQ=255)
   gcgGGCGCGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTaa                                                                                                                                                  <  1:1337751/150‑1 (MQ=255)
    cgGGCGCGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTaaa                                                                                                                                                 <  1:563121/150‑1 (MQ=255)
    cgGGCGCGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTaaa                                                                                                                                                 >  2:1629737/1‑150 (MQ=255)
     gggCGCGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTaaaa                                                                                                                                                >  1:1075780/1‑150 (MQ=255)
      ggCGCGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAAt                                                                                                                                               <  2:3127724/150‑1 (MQ=255)
       gcgcGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATa                                                                                                                                              <  1:972748/150‑1 (MQ=255)
         gcgGCTTTCCGGGTTCTGACACCCGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACt                                                                                                                                            <  2:2804744/150‑1 (MQ=255)
           ggCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACttt                                                                                                                                          <  1:2537826/150‑1 (MQ=255)
             cTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCg                                                                                                                                        <  1:4073778/150‑1 (MQ=255)
              tttCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGa                                                                                                                                       >  2:4230227/1‑150 (MQ=255)
               ttCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGaa                                                                                                                                      <  2:481340/150‑1 (MQ=255)
                tCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTATCGAAt                                                                                                                                     >  1:3669672/1‑150 (MQ=255)
                   gggTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGaa                                                                                                                                  <  2:2952280/150‑1 (MQ=255)
                    ggTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGaaa                                                                                                                                 <  2:740466/150‑1 (MQ=255)
                      ttCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGt                                                                                                                               <  1:401243/150‑1 (MQ=255)
                        cTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTa                                                                                                                             <  2:2891214/150‑1 (MQ=255)
                        cTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTa                                                                                                                             <  1:3264079/150‑1 (MQ=255)
                          gACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGa                                                                                                                           <  1:3400317/150‑1 (MQ=255)
                                    gCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCg                                                                                                                 <  1:2571498/150‑1 (MQ=255)
                                    gCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCg                                                                                                                 <  1:619940/150‑1 (MQ=255)
                                         ccAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCTGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAATGTTAAATTGATGTGCGTCAAc                                                                                                            <  2:1066981/150‑1 (MQ=255)
                                         ccAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAAc                                                                                                            <  2:2112834/150‑1 (MQ=255)
                                           aGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTg                                                                                                          <  1:523522/150‑1 (MQ=255)
                                               aaCAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCa                                                                                                      <  1:772700/150‑1 (MQ=255)
                                               aaCAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCa                                                                                                      >  2:2914048/1‑150 (MQ=255)
                                                aCAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCag                                                                                                     <  2:1059677/150‑1 (MQ=255)
                                                aCAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCag                                                                                                     >  2:4438668/1‑150 (MQ=255)
                                                    ccTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCagagag                                                                                                 <  2:4252862/150‑1 (MQ=255)
                                                      tttAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTAGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGtt                                                                                               <  2:2656356/150‑1 (MQ=255)
                                                        tAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGtttt                                                                                             >  1:1960707/1‑150 (MQ=255)
                                                         aaGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCTAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTc                                                                                            <  2:448024/150‑1 (MQ=255)
                                                         aaGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTc                                                                                            >  2:4298917/1‑150 (MQ=255)
                                                          aGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTcc                                                                                           <  1:698441/150‑1 (MQ=255)
                                                          aGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTcc                                                                                           <  1:1629737/150‑1 (MQ=255)
                                                              aTACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTg                                                                                       <  1:1572772/150‑1 (MQ=255)
                                                                aCGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGAt                                                                                     <  1:4474419/150‑1 (MQ=255)
                                                                 cGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATa                                                                                    <  1:381962/150‑1 (MQ=255)
                                                                  gACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAg                                                                                   <  1:4206413/150‑1 (MQ=255)
                                                                  gACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAg                                                                                   >  1:4518327/1‑150 (MQ=255)
                                                                   aCCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGt                                                                                  <  2:1613021/150‑1 (MQ=255)
                                                                     cGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCt                                                                                <  2:3387832/150‑1 (MQ=255)
                                                                       tGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTAc                                                                              <  1:4312738/150‑1 (MQ=255)
                                                                         cGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACAt                                                                            <  1:1670978/150‑1 (MQ=255)
                                                                          ggATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACAtt                                                                           >  2:198353/1‑150 (MQ=255)
                                                                            aTACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCa                                                                         >  1:1093861/1‑150 (MQ=255)
                                                                            aTACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCa                                                                         >  1:3055475/1‑150 (MQ=255)
                                                                             tACCGGGGCCATCATGGGTCTAGTAGCGATGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTTATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAg                                                                        <  2:4125116/150‑1 (MQ=255)
                                                                                 ggggCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACaa                                                                    >  1:1491523/1‑150 (MQ=255)
                                                                                       atcatGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTa                                                              >  1:2671992/1‑150 (MQ=255)
                                                                                         catGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACa                                                            <  1:3433581/150‑1 (MQ=255)
                                                                                         catGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACa                                                            <  1:3432832/150‑1 (MQ=255)
                                                                                         catGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACa                                                            <  1:1160157/150‑1 (MQ=255)
                                                                                         catGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACa                                                            >  2:3861798/1‑150 (MQ=255)
                                                                                         catGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACa                                                            <  1:3433633/150‑1 (MQ=255)
                                                                                           tGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTAAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTAAAtt                                                          >  2:3260768/1‑150 (MQ=255)
                                                                                            gggTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACAttt                                                         <  1:478248/150‑1 (MQ=255)
                                                                                             ggTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACAtttt                                                        >  1:2175612/1‑150 (MQ=255)
                                                                                             ggTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACAtttt                                                        <  1:3140974/150‑1 (MQ=255)
                                                                                              gTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTATAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCTGACAAAAAGTACATTTTg                                                       <  2:4406655/150‑1 (MQ=255)
                                                                                                   gTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGAt                                                  >  2:120201/1‑150 (MQ=255)
                                                                                                   gTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGAt                                                  <  1:2239835/150‑1 (MQ=255)
                                                                                                     aGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACAGTTTGAGGATgg                                                >  1:3290326/1‑150 (MQ=255)
                                                                                                       cgcTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGtt                                              <  1:1471924/150‑1 (MQ=255)
                                                                                                        gcTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTa                                             >  1:1270002/1‑150 (MQ=255)
                                                                                                        gcTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACAGTTTGAGGATGGTTa                                             >  1:3575487/1‑150 (MQ=255)
                                                                                                         cTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTAt                                            >  1:1087361/1‑150 (MQ=255)
                                                                                                         cTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTAt                                            <  2:1467200/150‑1 (MQ=255)
                                                                                                         cTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTAt                                            >  2:1699469/1‑150 (MQ=255)
                                                                                                           gAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATgg                                          >  2:1739750/1‑150 (MQ=255)
                                                                                                               atTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATGTTGAGGATGGTTATGGAACt                                      >  2:2513847/1‑150 (MQ=255)
                                                                                                                 tGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGt                                    <  1:4438668/150‑1 (MQ=255)
                                                                                                                  gAAAATCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTa                                   >  2:2279364/1‑150 (MQ=255)
                                                                                                                   aaaaTCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTAt                                  >  1:1745998/1‑150 (MQ=255)
                                                                                                                   aaaaTCATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTAt                                  >  1:1745986/1‑150 (MQ=255)
                                                                                                                    aaaTCATAGTTGCCTCTCTATTTAGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATc                                 <  2:3213885/150‑1 (MQ=255)
                                                                                                                     aaTCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCt                                <  2:3052853/150‑1 (MQ=255)
                                                                                                                        cATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGa                             >  1:1816700/1‑150 (MQ=255)
                                                                                                                        cATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGa                             >  2:1936399/1‑150 (MQ=255)
                                                                                                                        cATAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGa                             <  1:2555345/150‑1 (MQ=255)
                                                                                                                         aTAGTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGAt                            <  2:1024792/150‑1 (MQ=255)
                                                                                                                         aTAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGAt                            >  2:1108775/1‑150 (MQ=255)
                                                                                                                         aTAGTTGCCTCTCTATTTCGCTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGAt                            >  2:1108762/1‑150 (MQ=255)
                                                                                                                          tcgTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGAGAGTCTACATTCAGACAATAAGTTCATTTTGAGGATGGTTATGGAACTGTATCTGGATa                           <  2:3664524/148‑1 (MQ=255)
                                                                                                                            gTTGCCTCTCTATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACt                         <  1:352349/150‑1 (MQ=255)
                                                                                                                                ccTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAg                     <  1:2788861/150‑1 (MQ=255)
                                                                                                                                 ctctctATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGa                    <  1:4290242/150‑1 (MQ=255)
                                                                                                                                 ctctctATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGa                    >  1:4455923/1‑150 (MQ=255)
                                                                                                                                   ctctATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACg                  >  2:2961219/1‑150 (MQ=255)
                                                                                                                                    tctATTTCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACgt                 <  2:1048401/150‑1 (MQ=255)
                                                                                                                                          tCGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACGTTGTTGc           <  2:3769831/150‑1 (MQ=255)
                                                                                                                                           cGTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACGTTGTTGCg          >  2:1628713/1‑150 (MQ=255)
                                                                                                                                            gTTCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACGTTGTTGCgg         >  2:4204328/1‑150 (MQ=255)
                                                                                                                                              tCAAGTATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACGTTGTTGCGGTg       <  2:3343595/150‑1 (MQ=255)
                                                                                                                                              tCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAGCTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACGTTGTTGCGGTg       <  2:1411010/150‑1 (MQ=255)
                                                                                                                                               cAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACGTTGTTGCGGTGa      >  1:1992050/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CGTGCGGGCGCGGCTTTCCGGGTTCTGACACCAGCGGCAGCCCAGCGAACAGCCTTTAAGAAATACGACCGTGCGGATACCGGGGCCATCATGGGTCGAGTAGCGCTGAATATTGAAAATCATAGTTGCCTCTCTATTTCGTTCAAGCATTAAAATACTTTCGAATGAAAGTTAGATTGATGTGCGTCAACTGTTCAGAGAGTTTTCCCGTGATAGTCTACATTCAGACAAAAAGTACATTTTGAGGATGGTTATGGAACTGTATCTGGATACTTCAGACGTTGTTGCGGTGAAGGC  >  CP009273/858845‑859141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A