Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,268,535 A→T 20.1% S191S (TCA→TCT)  chaC → cation transport regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,268,5350AT20.1% 732.5 / 185.3 304S191S (TCA→TCT) chaCcation transport regulator
Reads supporting (aligned to +/- strand):  ref base A (130/112);  new base T (34/27);  total (164/140)
Fisher's exact test for biased strand distribution p-value = 8.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.22e-01

Only 100 of 308 total aligned reads displayed.

TTGATGATGGACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTC  >  CP009273/1268387‑1268684
                                                                                                                                                    |                                                                                                                                                     
ttgatGATGGACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCAc                                                                                                                                                      <  1:2050597/150‑1 (MQ=255)
    tgatgGACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGa                                                                                                                                                  <  2:2973078/150‑1 (MQ=255)
     gatgGACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCTCTGGaa                                                                                                                                                 >  2:3863015/1‑150 (MQ=255)
     gatgGACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGaa                                                                                                                                                 >  1:3805355/1‑150 (MQ=255)
      atgGACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGACCCAACGCACAATACCTGTTTTCTCTGGAAc                                                                                                                                                >  2:2906546/1‑150 (MQ=255)
      atgGACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCTCTGGAAc                                                                                                                                                >  2:1656605/1‑150 (MQ=255)
         gACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCTCTGGAACAgg                                                                                                                                             <  2:3501298/150‑1 (MQ=255)
          aCGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGa                                                                                                                                            >  2:705536/1‑150 (MQ=255)
           cGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAg                                                                                                                                           <  1:1322193/150‑1 (MQ=255)
             cacaGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCt                                                                                                                                         >  1:3463134/1‑150 (MQ=255)
              acaGTACACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCtt                                                                                                                                        <  1:832201/150‑1 (MQ=255)
              acaGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCtt                                                                                                                                        <  1:3240072/150‑1 (MQ=255)
                aGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCTCTGGAACAGGAGCTTAt                                                                                                                                      <  2:1507812/150‑1 (MQ=255)
                   aaaCGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCaa                                                                                                                                   >  1:36683/1‑150 (MQ=255)
                     aCGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAAc                                                                                                                                 >  2:690572/1‑150 (MQ=255)
                         cATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGTGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTggg                                                                                                                             <  1:4157892/150‑1 (MQ=255)
                         cATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTggg                                                                                                                             >  1:3901432/1‑150 (MQ=255)
                           ttGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGaa                                                                                                                           <  2:4145205/150‑1 (MQ=255)
                              tgtTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGc                                                                                                                        >  1:852227/1‑150 (MQ=255)
                               gtTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCa                                                                                                                       <  1:147741/150‑1 (MQ=255)
                                tttATTATGGACCCGCGACATCCAGAATATNAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAg                                                                                                                      >  1:4280369/1‑150 (MQ=255)
                                 ttattaTGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAgg                                                                                                                     <  2:328927/150‑1 (MQ=255)
                                  tattatGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGa                                                                                                                    <  2:3250458/150‑1 (MQ=255)
                                     tatGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGa                                                                                                                 >  2:1799833/1‑150 (MQ=255)
                                      atGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCTCTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGAt                                                                                                                <  2:537307/150‑1 (MQ=255)
                                      atGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCTCTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGAt                                                                                                                <  2:1384266/150‑1 (MQ=255)
                                       tGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATg                                                                                                               >  2:815794/1‑150 (MQ=255)
                                         gACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATggg                                                                                                             <  2:3537660/150‑1 (MQ=255)
                                          aCCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGc                                                                                                            >  2:3915593/1‑150 (MQ=255)
                                           cccGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAACCTGGGAATGCAGGACGATGGGCt                                                                                                           <  2:605082/150‑1 (MQ=255)
                                           cccGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCt                                                                                                           <  1:3134000/150‑1 (MQ=255)
                                           cccGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCt                                                                                                           <  1:2177622/150‑1 (MQ=255)
                                               cgACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAAt                                                                                                       >  2:2159177/1‑150 (MQ=255)
                                               cgACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAAt                                                                                                       >  2:245116/1‑150 (MQ=255)
                                                gACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCTCTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATg                                                                                                      >  2:210701/1‑150 (MQ=255)
                                                gACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCTCTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATg                                                                                                      >  2:3624742/1‑150 (MQ=255)
                                                gACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATg                                                                                                      <  1:2321517/150‑1 (MQ=255)
                                                  cATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGAt                                                                                                    >  1:1260451/1‑150 (MQ=255)
                                                  cATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGAt                                                                                                    <  1:1080999/150‑1 (MQ=255)
                                                  cATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGAt                                                                                                    <  1:882536/150‑1 (MQ=255)
                                                   aTCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGAtt                                                                                                   >  2:672650/1‑150 (MQ=255)
                                                   aTCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGAtt                                                                                                   >  1:1846679/1‑150 (MQ=255)
                                                    tCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGAttt                                                                                                  <  1:1856459/150‑1 (MQ=255)
                                                      cAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGc                                                                                                >  1:4177149/1‑150 (MQ=255)
                                                          atatGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCTCTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGt                                                                                            >  1:4149915/1‑150 (MQ=255)
                                                           tatGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCTCTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTa                                                                                           <  2:3905874/150‑1 (MQ=255)
                                                            atGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTAt                                                                                          >  1:400212/1‑150 (MQ=255)
                                                              gAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCg                                                                                        >  2:2521605/1‑150 (MQ=255)
                                                               aaTCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTCTCgg                                                                                       >  1:2236998/1‑150 (MQ=255)
                                                                aTCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCTCTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGt                                                                                      >  1:1196486/1‑150 (MQ=255)
                                                                    gATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCTCTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTaaaa                                                                                  >  1:2031895/1‑150 (MQ=255)
                                                                    gATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTaaaa                                                                                  <  2:1611959/150‑1 (MQ=255)
                                                                      tACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTaaaaaa                                                                                >  2:2275767/1‑150 (MQ=255)
                                                                       aCTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTaaaaaaa                                                                               >  1:1006534/1‑150 (MQ=255)
                                                                          cgcgCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCTCTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAActg                                                                            <  1:4636874/150‑1 (MQ=255)
                                                                          cgcgCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCTCTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAActg                                                                            <  1:1736443/150‑1 (MQ=255)
                                                                          cgcgCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAActg                                                                            <  1:4469666/150‑1 (MQ=255)
                                                                          cgcgCTCAGGTCATCGCGCCGATGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCTGTAAAAAAActg                                                                            >  2:4208366/1‑150 (MQ=255)
                                                                            cgcTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCTCTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAActgct                                                                          <  2:2576331/150‑1 (MQ=255)
                                                                            cgcTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAActgct                                                                          <  2:124284/150‑1 (MQ=255)
                                                                                 aGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCgg                                                                     >  2:1027689/1‑150 (MQ=255)
                                                                                   gTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGgag                                                                   >  1:3022707/1‑150 (MQ=255)
                                                                                    tCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCTCTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGgaga                                                                  >  2:751845/1‑150 (MQ=255)
                                                                                    tCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGgaga                                                                  >  2:4189926/1‑150 (MQ=255)
                                                                                    tCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGgaga                                                                  >  1:1379177/1‑150 (MQ=255)
                                                                                     cATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGaa                                                                 >  2:3050587/1‑150 (MQ=255)
                                                                                      aTCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCTCTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAAt                                                                >  2:4047627/1‑150 (MQ=255)
                                                                                        cgcgCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCTCTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAAttt                                                              >  2:1547280/1‑150 (MQ=255)
                                                                                        cgcgCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAAttt                                                              >  1:3543675/1‑150 (MQ=255)
                                                                                        cgcgCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAAttt                                                              <  2:4364134/150‑1 (MQ=255)
                                                                                             cGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCTCTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCgg                                                         <  2:1332414/150‑1 (MQ=255)
                                                                                               ttGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCTCTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGAt                                                       >  1:2441906/1‑150 (MQ=255)
                                                                                                tGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCTCTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATg                                                      <  2:3877312/150‑1 (MQ=255)
                                                                                                tGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATg                                                      >  2:1217987/1‑150 (MQ=255)
                                                                                                   ttGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGgtg                                                   <  2:2479177/150‑1 (MQ=255)
                                                                                                     gcggcggcgAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGgtgtg                                                 <  1:2118744/150‑1 (MQ=255)
                                                                                                      cggcggcgAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGgtgtgt                                                >  1:272435/1‑150 (MQ=255)
                                                                                                        gcggcgAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCTCTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTa                                              <  1:596712/150‑1 (MQ=255)
                                                                                                                  gTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGAt                                    >  2:1546875/1‑150 (MQ=255)
                                                                                                                   tCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGAtt                                   <  2:2786935/150‑1 (MQ=255)
                                                                                                                    ccGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTc                                  >  1:2893747/1‑150 (MQ=255)
                                                                                                                    ccGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTc                                  <  1:258047/150‑1 (MQ=255)
                                                                                                                     cGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCg                                 <  2:1060378/150‑1 (MQ=255)
                                                                                                                          ggAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGa                            >  2:4503299/1‑150 (MQ=255)
                                                                                                                           gAACCAACGCACAATACCTGTTTTCTCTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAg                           <  1:2815991/150‑1 (MQ=255)
                                                                                                                              ccAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTaa                        <  1:4066437/150‑1 (MQ=255)
                                                                                                                               cAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTaaa                       >  1:4377777/1‑150 (MQ=255)
                                                                                                                                aaCGCACAATACCTGTTTTCTCTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAAc                      >  2:2357566/1‑150 (MQ=255)
                                                                                                                                aaCGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAAc                      <  1:1056628/150‑1 (MQ=255)
                                                                                                                                  cGCACAATACCTGTTTTCACTGGAACAGGAGATTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCGGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACtt                    <  2:1017757/150‑1 (MQ=255)
                                                                                                                                    cacaATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTcc                  >  1:64491/1‑150 (MQ=255)
                                                                                                                                     acaATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTccc                 <  2:1260451/150‑1 (MQ=255)
                                                                                                                                       aaTACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGc               >  2:3881188/1‑150 (MQ=255)
                                                                                                                                       aaTACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGc               >  2:3120964/1‑150 (MQ=255)
                                                                                                                                       aaTACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGc               >  2:1733721/1‑150 (MQ=255)
                                                                                                                                          aCCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATa            >  2:1979876/1‑150 (MQ=255)
                                                                                                                                             tGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTg         <  1:1587132/150‑1 (MQ=255)
                                                                                                                                              gTTTTCTCTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTgg        <  2:1749740/150‑1 (MQ=255)
                                                                                                                                                tttCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTgggg      <  1:3553730/150‑1 (MQ=255)
                                                                                                                                                    aCTGGAACAGGAGCTTATCAAACTGTGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTc  <  2:1558736/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TTGATGATGGACGCACAGTAAACGCCATTGTGTTTATTATGGACCCGCGACATCCAGAATATGAATCTGATACTCGCGCTCAGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTC  >  CP009273/1268387‑1268684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A