Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,277,786 G→A 100% D823N (GAC→AAC)  narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,277,7860GA98.8% 835.3 / ‑0.2 240D823N (GAC→AAC) narGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (0/3);  new base A (123/114);  total (123/117)
Fisher's exact test for biased strand distribution p-value = 1.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 240 total aligned reads displayed.

TCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGG  >  CP009273/1277640‑1277929
                                                                                                                                                  |                                                                                                                                               
tCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACt                                                                                                                                              >  2:2228602/1‑150 (MQ=255)
 ccGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTg                                                                                                                                             >  1:4171472/1‑150 (MQ=255)
   gTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTggg                                                                                                                                           <  1:2780033/150‑1 (MQ=255)
   gTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTggg                                                                                                                                           <  1:1040905/150‑1 (MQ=255)
   gTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGAGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTggg                                                                                                                                           <  1:2864236/150‑1 (MQ=255)
    tCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGa                                                                                                                                          <  1:542542/150‑1 (MQ=255)
    tCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGa                                                                                                                                          <  1:981041/150‑1 (MQ=255)
     cTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGaa                                                                                                                                         >  2:1885120/1‑150 (MQ=255)
      tGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGaaa                                                                                                                                        <  2:4508431/150‑1 (MQ=255)
        tCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATc                                                                                                                                      >  2:2204380/1‑150 (MQ=255)
        tCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATc                                                                                                                                      >  1:979113/1‑150 (MQ=255)
         cGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCt                                                                                                                                     >  2:2811725/1‑150 (MQ=255)
         cGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCAGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCt                                                                                                                                     >  1:4535197/1‑150 (MQ=255)
          gAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTa                                                                                                                                    >  2:209100/1‑150 (MQ=255)
          gAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTa                                                                                                                                    >  1:3503318/1‑150 (MQ=255)
          gAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTa                                                                                                                                    >  2:2672060/1‑150 (MQ=255)
             cACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACaa                                                                                                                                 <  1:408606/150‑1 (MQ=255)
              aCCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACaaa                                                                                                                                <  2:2114158/150‑1 (MQ=255)
              aCCTGTATCTTTTCCGACATCATTTTGCCGACGGCGACCTGTTACGAAAAAGACGACATGTATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACata                                                                                                                                <  2:610582/150‑3 (MQ=25)
               ccTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAg                                                                                                                               <  1:1411919/150‑1 (MQ=255)
               ccTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAg                                                                                                                               >  1:2840189/1‑150 (MQ=255)
                cTGTCTCTATTCCGACATCATATTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGAGGCGGTCGATCCGGCCTGGGAAGCGAAAAGAAACTGGGAAATCTACAAAGc                                                                                                                              >  1:2211303/1‑150 (MQ=255)
                   tctcTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCAt                                                                                                                           <  2:4232202/150‑1 (MQ=255)
                             gACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGaaa                                                                                                                 <  1:1193412/150‑1 (MQ=255)
                             gACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGaaa                                                                                                                 <  2:3567136/150‑1 (MQ=255)
                             gACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGaaa                                                                                                                 <  1:75674/150‑1 (MQ=255)
                                atcatTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATtc                                                                                                              >  1:2291401/1‑150 (MQ=255)
                                 tcatTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATtct                                                                                                             <  2:3641098/150‑1 (MQ=255)
                                  catTATGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCAGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATtctc                                                                                                            >  1:3164468/1‑150 (MQ=255)
                                   atTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTcc                                                                                                           <  2:157392/150‑1 (MQ=255)
                                   atTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTcc                                                                                                           <  1:2687880/150‑1 (MQ=255)
                                   atTTTGACGACGGCGAACTTGTACGAAAAAGACGTCATTTATACTTCGGATATGCTTACGTTTATTCACCCGCTGTCTGCTGAGGTCGATCCGTACTGGGAAGCGAAAAGCAACTGGGAAATATTCAAATCATTCGCGAAGTAATTCTcc                                                                                                           <  2:560504/150‑1 (MQ=255)
                                       tGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAg                                                                                                       >  2:4121889/1‑150 (MQ=255)
                                        gCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAgt                                                                                                      <  1:215612/150‑1 (MQ=255)
                                          cGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAgtgt                                                                                                    <  1:2467474/150‑1 (MQ=255)
                                          cGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAgtgt                                                                                                    <  2:4641925/150‑1 (MQ=255)
                                          cGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAgtgt                                                                                                    <  2:3157149/150‑1 (MQ=255)
                                          cGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAgtgt                                                                                                    <  1:1257883/150‑1 (MQ=255)
                                            aCGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGc                                                                                                  >  2:485707/1‑150 (MQ=255)
                                              ggCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGt                                                                                                >  2:736028/1‑150 (MQ=255)
                                              ggCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGt                                                                                                >  2:3378227/1‑150 (MQ=255)
                                              ggCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGt                                                                                                >  1:2608729/1‑150 (MQ=255)
                                               gCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACCAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTc                                                                                               <  2:4056338/150‑1 (MQ=255)
                                               gCGACCTGGTACGAAAAAGACGACATGAATAATTAGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGATGTGTGCGTc                                                                                               <  1:1123998/150‑1 (MQ=255)
                                                 gACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCgg                                                                                             >  1:4424358/1‑150 (MQ=255)
                                                  aCCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGc                                                                                            >  2:3380977/1‑150 (MQ=255)
                                                    cTGGTACGAACAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCa                                                                                          <  2:2406084/150‑1 (MQ=255)
                                                    cTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCa                                                                                          <  2:2406812/150‑1 (MQ=255)
                                                    cTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCa                                                                                          >  1:1735870/1‑150 (MQ=255)
                                                       gTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCt                                                                                       >  1:3079096/1‑150 (MQ=255)
                                                        tACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTg                                                                                      >  2:4404520/1‑150 (MQ=255)
                                                        tACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTg                                                                                      >  1:2271767/1‑150 (MQ=255)
                                                         ccGAAAAAGACGCCCGGAATCCTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTgg                                                                                     <  1:1618194/149‑1 (MQ=25)
                                                         aCGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTgg                                                                                     <  2:1500820/150‑1 (MQ=255)
                                                            aaaaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTa                                                                                  >  2:392758/1‑150 (MQ=255)
                                                            aaaaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTa                                                                                  <  2:595114/150‑1 (MQ=255)
                                                             aaaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTaa                                                                                 >  1:3114446/1‑150 (MQ=255)
                                                             aaaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTaa                                                                                 >  1:3114340/1‑150 (MQ=255)
                                                             aaaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTaa                                                                                 <  2:644787/150‑1 (MQ=255)
                                                               aaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAg                                                                               <  2:3402290/150‑1 (MQ=255)
                                                                   cgacATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAAc                                                                           <  2:2061031/150‑1 (MQ=255)
                                                                   cgacATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAAc                                                                           >  1:3626744/1‑150 (MQ=255)
                                                                       aTGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGAc                                                                       <  1:2015654/150‑1 (MQ=255)
                                                                        tGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACa                                                                      <  1:3264272/150‑1 (MQ=255)
                                                                          aaTACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATc                                                                    >  1:4105772/1‑150 (MQ=255)
                                                                                tCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACg                                                              >  1:4632342/1‑150 (MQ=255)
                                                                                tCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACg                                                              <  1:3558205/150‑1 (MQ=255)
                                                                                    atatGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGc                                                          >  2:13492/1‑150 (MQ=255)
                                                                                    atatGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGc                                                          >  1:620075/1‑150 (MQ=255)
                                                                                    atatGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGc                                                          >  2:3889539/1‑150 (MQ=255)
                                                                                      atGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCt                                                        <  1:3007498/150‑1 (MQ=255)
                                                                                        gCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTAt                                                      >  2:204278/1‑150 (MQ=255)
                                                                                           tCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCa                                                   >  2:3298389/1‑150 (MQ=255)
                                                                                              gTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCa                                                >  2:2977135/1‑150 (MQ=255)
                                                                                                 tATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGa                                             >  2:946026/1‑150 (MQ=255)
                                                                                                 tATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGa                                             >  2:947082/1‑150 (MQ=255)
                                                                                                  aTTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGAc                                            >  2:1787568/1‑150 (MQ=255)
                                                                                                   ttCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAGAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGAct                                           <  2:4104114/150‑1 (MQ=255)
                                                                                                   ttCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGAct                                           <  2:4538285/150‑1 (MQ=255)
                                                                                                     cACCCGCTGTCTGCGGCGGACGAACCGGCCGGGGAAGCGAAAAGAAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTAAGAAGTGTGCGTCGTCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGActct                                         >  1:1902825/1‑150 (MQ=255)
                                                                                                      aCCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTg                                        <  1:1365209/150‑1 (MQ=255)
                                                                                                      aCCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTg                                        <  1:4236810/150‑1 (MQ=255)
                                                                                                         cGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCg                                     >  2:4164819/1‑150 (MQ=255)
                                                                                                            tGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTg                                  >  2:1595882/1‑150 (MQ=255)
                                                                                                                tGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACt                              <  1:2313865/150‑1 (MQ=255)
                                                                                                                      ggTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCa                        >  2:1200544/1‑150 (MQ=255)
                                                                                                                       gTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAg                       >  1:2017524/1‑150 (MQ=255)
                                                                                                                         cGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGcc                     <  1:737506/150‑1 (MQ=255)
                                                                                                                          gATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCg                    >  2:1730076/1‑150 (MQ=255)
                                                                                                                             ccGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTg                 <  2:2508283/150‑1 (MQ=255)
                                                                                                                               ggCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGa               >  1:3817979/1‑150 (MQ=255)
                                                                                                                                 ccTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGAtg             >  2:607237/1‑150 (MQ=255)
                                                                                                                                  cTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGAtgt            <  2:2991701/150‑1 (MQ=255)
                                                                                                                                     ggAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGaa         <  2:1014414/150‑1 (MQ=255)
                                                                                                                                      gAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGaaa        <  2:4515663/150‑1 (MQ=255)
                                                                                                                                       aaGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAg       >  2:4565402/1‑150 (MQ=255)
                                                                                                                                        aGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGACCTGGCGCAGCCGCTGGATGTGAAAGa      >  1:1291607/1‑150 (MQ=255)
                                                                                                                                        aGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGa      >  2:3123489/1‑150 (MQ=255)
                                                                                                                                         gCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGAc     >  1:1141303/1‑150 (MQ=255)
                                                                                                                                            aaaaGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTgg  >  2:2301525/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
TCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGG  >  CP009273/1277640‑1277929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACT  >  CP009273/1277637‑1277927
                                                                                                                                                     |                                                                                                                                             
ACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCA                                                                                                                                               <  SRR19973072.2510317/150‑1 (MQ=60)
   TCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGCTCGATCCGGCCTGGGAAGCGAAAAGCAACT                                                                                                                                            <  SRR19973072.2386411/150‑1 (MQ=60)
    CCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTG                                                                                                                                           >  SRR19973072.4180206/1‑150 (MQ=60)
      GTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGG                                                                                                                                         <  SRR19973072.1043151/150‑1 (MQ=60)
      GTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGG                                                                                                                                         <  SRR19973072.2785985/150‑1 (MQ=60)
      GTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGAGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGG                                                                                                                                         <  SRR19973072.2870377/150‑1 (MQ=60)
       TCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGA                                                                                                                                        <  SRR19973072.983192/150‑1 (MQ=60)
       TCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGA                                                                                                                                        <  SRR19973072.983153/150‑1 (MQ=60)
       TCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGA                                                                                                                                        <  SRR19973072.543826/150‑1 (MQ=60)
        CTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAA                                                                                                                                       >  SRR19973072.2860349/1‑150 (MQ=60)
           TCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATC                                                                                                                                    >  SRR19973072.981255/1‑150 (MQ=60)
            CGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCT                                                                                                                                   <  SRR19973072.2780035/150‑1 (MQ=60)
            CGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCAGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCT                                                                                                                                   >  SRR19973072.4544764/1‑150 (MQ=60)
             GAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTA                                                                                                                                  >  SRR19973072.3510661/1‑150 (MQ=60)
                CACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAA                                                                                                                               <  SRR19973072.409514/150‑1 (MQ=60)
                CACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAA                                                                                                                               <  SRR19973072.409590/150‑1 (MQ=60)
                CACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAA                                                                                                                               <  SRR19973072.409592/150‑1 (MQ=60)
                  CCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAG                                                                                                                             >  SRR19973072.2846265/1‑150 (MQ=60)
                  CCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAG                                                                                                                             >  SRR19973072.2846269/1‑150 (MQ=60)
                  CCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAG                                                                                                                             <  SRR19973072.1414991/150‑1 (MQ=60)
                   CTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGC                                                                                                                            >  SRR19973072.2216116/1‑150 (MQ=60)
                   CTGTCTCTATTCCGACATCATATTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGAGGCGGTCGATCCGGCCTGGGAAGCGAAAAGAAACTGGGAAATCTACAAAGC                                                                                                                            >  SRR19973072.2216108/1‑150 (MQ=60)
                      TCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCAT                                                                                                                         <  SRR19973072.2178456/150‑1 (MQ=60)
                           ATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGA                                                                                                                    >  SRR19973072.2615493/1‑150 (MQ=60)
                           ATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGA                                                                                                                    >  SRR19973072.2614794/1‑150 (MQ=60)
                                GACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAA                                                                                                               <  SRR19973072.75867/150‑1 (MQ=60)
                                GACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAA                                                                                                               <  SRR19973072.1195926/150‑1 (MQ=60)
                                GACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAA                                                                                                               <  SRR19973072.75719/150‑1 (MQ=60)
                                GACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAA                                                                                                               <  SRR19973072.1195938/150‑1 (MQ=60)
                                 ACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAAT                                                                                                              >  SRR19973072.319182/1‑150 (MQ=60)
                                 ACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAAT                                                                                                              <  SRR19973072.4595066/150‑1 (MQ=60)
                                   ATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTC                                                                                                            >  SRR19973072.2296343/1‑150 (MQ=60)
                                   ATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTC                                                                                                            >  SRR19973072.2296308/1‑150 (MQ=60)
                                   ATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTC                                                                                                            >  SRR19973072.3018848/1‑150 (MQ=60)
                                     CATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTC                                                                                                          <  SRR19973072.1148659/150‑1 (MQ=60)
                                     CATTATGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCAGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTC                                                                                                          >  SRR19973072.3171149/1‑150 (MQ=60)
                                      ATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCC                                                                                                         <  SRR19973072.2693605/150‑1 (MQ=60)
                                      ATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCC                                                                                                         <  SRR19973072.966133/150‑1 (MQ=60)
                                      ATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCC                                                                                                         <  SRR19973072.3124124/150‑1 (MQ=60)
                                      ATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCC                                                                                                         <  SRR19973072.149830/150‑1 (MQ=60)
                                      ATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCC                                                                                                         <  SRR19973072.149948/150‑1 (MQ=60)
                                           GCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGT                                                                                                    <  SRR19973072.216155/150‑1 (MQ=60)
                                             CGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGTTCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGT                                                                                                  <  SRR19973072.1260639/150‑1 (MQ=60)
                                             CGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGT                                                                                                  <  SRR19973072.3589586/150‑1 (MQ=60)
                                             CGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGT                                                                                                  <  SRR19973072.2472837/150‑1 (MQ=60)
                                             CGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGT                                                                                                  <  SRR19973072.1260588/150‑1 (MQ=60)
                                              GACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTG                                                                                                 <  SRR19973072.2063029/150‑1 (MQ=60)
                                                 GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGT                                                                                              >  SRR19973072.2614350/1‑150 (MQ=60)
                                                  GCGACCTGGTACGAAAAAGACGACATGAATAATTAGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGATGTGTGCGTC                                                                                             <  SRR19973072.1126361/150‑1 (MQ=60)
                                                    GACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGG                                                                                           >  SRR19973072.1105932/1‑150 (MQ=60)
                                                    GACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGG                                                                                           >  SRR19973072.2901644/1‑150 (MQ=60)
                                                    GACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGG                                                                                           >  SRR19973072.3552162/1‑150 (MQ=60)
                                                    GACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGG                                                                                           >  SRR19973072.4433664/1‑150 (MQ=60)
                                                    GACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGG                                                                                           >  SRR19973072.4433680/1‑150 (MQ=60)
                                                       CTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCA                                                                                        >  SRR19973072.1739890/1‑150 (MQ=60)
                                                       CTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCA                                                                                        >  SRR19973072.1739672/1‑150 (MQ=60)
                                                          GTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCT                                                                                     >  SRR19973072.3085596/1‑150 (MQ=60)
                                                           TACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTG                                                                                    >  SRR19973072.2276672/1‑150 (MQ=60)
                                                           TACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTG                                                                                    >  SRR19973072.2276647/1‑150 (MQ=60)
                                                           TACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTG                                                                                    <  SRR19973072.3133565/150‑1 (MQ=60)
                                                            CCGAAAAAGACGCCCGGAATCCTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGG                                                                                   <  SRR19973072.1621706/150‑1 (MQ=60)
                                                              GAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGT                                                                                 >  SRR19973072.2225852/1‑150 (MQ=60)
                                                               AAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTA                                                                                >  SRR19973072.2093020/1‑150 (MQ=60)
                                                                AAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAA                                                                               >  SRR19973072.2458736/1‑150 (MQ=60)
                                                                AAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAA                                                                               >  SRR19973072.3120907/1‑150 (MQ=60)
                                                                AAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAA                                                                               >  SRR19973072.3121003/1‑150 (MQ=60)
                                                                AAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAA                                                                               >  SRR19973072.3121013/1‑150 (MQ=60)
                                                                    GACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAA                                                                           <  SRR19973072.716879/150‑1 (MQ=60)
                                                                    GACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAA                                                                           <  SRR19973072.3097956/150‑1 (MQ=60)
                                                                      CGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAAC                                                                         >  SRR19973072.3634388/1‑150 (MQ=60)
                                                                          ATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGAC                                                                     <  SRR19973072.2020004/150‑1 (MQ=60)
                                                                          ATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGAC                                                                     >  SRR19973072.4402998/1‑150 (MQ=60)
                                                                           TGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACA                                                                    <  SRR19973072.3271200/150‑1 (MQ=60)
                                                                             AATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATC                                                                  >  SRR19973072.4114395/1‑150 (MQ=60)
                                                                                  TTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCAC                                                             >  SRR19973072.1742298/1‑150 (MQ=60)
                                                                                   TCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACG                                                            >  SRR19973072.4642038/1‑150 (MQ=60)
                                                                                   TCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACG                                                            <  SRR19973072.3565687/150‑1 (MQ=60)
                                                                                     GGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCT                                                          >  SRR19973072.562105/1‑150 (MQ=60)
                                                                                       ATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGC                                                        >  SRR19973072.621496/1‑150 (MQ=60)
                                                                                         ATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAACGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCT                                                      <  SRR19973072.1402528/150‑1 (MQ=60)
                                                                                         ATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCT                                                      <  SRR19973072.3013892/150‑1 (MQ=60)
                                                                                              TCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCA                                                 >  SRR19973072.449765/1‑150 (MQ=60)
                                                                                                 GTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCA                                              <  SRR19973072.3320860/150‑1 (MQ=60)
                                                                                                  TTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCAT                                             <  SRR19973072.3897623/150‑1 (MQ=60)
                                                                                                        CACCCGCTGTCTGCGGCGGACGAACCGGCCGGGGAAGCGAAAAGAAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTAAGAAGTGTGCGTCGTCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCT                                       >  SRR19973072.1906886/1‑150 (MQ=60)
                                                                                                         ACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTG                                      <  SRR19973072.4245633/150‑1 (MQ=60)
                                                                                                         ACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTG                                      <  SRR19973072.1368193/150‑1 (MQ=60)
                                                                                                         ACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTG                                      <  SRR19973072.3497189/150‑1 (MQ=60)
                                                                                                           CCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCC                                    <  SRR19973072.1987713/150‑1 (MQ=60)
                                                                                                            CGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCG                                   >  SRR19973072.984298/1‑150 (MQ=60)
                                                                                                                   TGCGGCGGTCTATCCGGCCTGGGAAGCTAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACT                            <  SRR19973072.3691566/150‑1 (MQ=60)
                                                                                                                   TGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACT                            >  SRR19973072.2503420/1‑150 (MQ=60)
                                                                                                                   TGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACT                            <  SRR19973072.2318803/150‑1 (MQ=60)
                                                                                                                         GGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCA                      >  SRR19973072.489092/1‑150 (MQ=60)
                                                                                                                          GTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAATCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAG                     >  SRR19973072.3776739/1‑150 (MQ=60)
                                                                                                                          GTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAG                     <  SRR19973072.4557429/150‑1 (MQ=60)
                                                                                                                          GTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAG                     >  SRR19973072.2021875/1‑150 (MQ=60)
                                                                                                                          GTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAG                     >  SRR19973072.2021884/1‑150 (MQ=60)
                                                                                                                            CGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCC                   <  SRR19973072.4159911/150‑1 (MQ=60)
                                                                                                                            CGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCC                   <  SRR19973072.739088/150‑1 (MQ=60)
                                                                                                                            CGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCC                   <  SRR19973072.739080/150‑1 (MQ=60)
                                                                                                                            CGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCC                   <  SRR19973072.1677352/150‑1 (MQ=60)
                                                                                                                             GATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCG                  >  SRR19973072.1275432/1‑150 (MQ=60)
                                                                                                                               TCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCT                <  SRR19973072.3042740/150‑1 (MQ=60)
                                                                                                                                 CGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGG              >  SRR19973072.4236217/1‑150 (MQ=60)
                                                                                                                                  GGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGA             >  SRR19973072.3825967/1‑150 (MQ=60)
                                                                                                                                  GGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGA             <  SRR19973072.120064/150‑1 (MQ=60)
                                                                                                                                    CCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATG           <  SRR19973072.2799818/150‑1 (MQ=60)
                                                                                                                                     CTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGT          <  SRR19973072.1313739/150‑1 (MQ=60)
                                                                                                                                           AGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGACCTGGCGCAGCCGCTGGATGTGAAAGA    >  SRR19973072.1294403/1‑150 (MQ=60)
                                                                                                                                            GCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGAC   >  SRR19973072.1143690/1‑150 (MQ=60)
                                                                                                                                            GCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGAC   >  SRR19973072.1143683/1‑150 (MQ=60)
                                                                                                                                             CGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACT  <  SRR19973072.947992/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                             
ACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACT  >  CP009273/1277637‑1277927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑