Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,277,786 G→A 100% D823N (GAC→AAC)  narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,277,7860GA99.6% 902.9 / ‑4.7 277D823N (GAC→AAC) narGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (149/127);  total (150/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 277 total aligned reads displayed.

TTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAA  >  CP009273/1277639‑1277931
                                                                                                                                                   |                                                                                                                                                 
 tCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACt                                                                                                                                                <  2:3388367/150‑1 (MQ=255)
 tCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACt                                                                                                                                                <  2:361707/150‑1 (MQ=255)
    gTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTggg                                                                                                                                             <  2:3303791/150‑1 (MQ=255)
    gTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTggg                                                                                                                                             <  1:407089/150‑1 (MQ=255)
      cTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGaa                                                                                                                                           >  2:2993907/1‑150 (MQ=255)
       tGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGaaa                                                                                                                                          >  1:3999495/1‑150 (MQ=255)
           gAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGACAAGCAACTGGGAAATCTa                                                                                                                                      >  1:4095195/1‑150 (MQ=255)
           gAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTa                                                                                                                                      <  1:419346/150‑1 (MQ=255)
           gAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTa                                                                                                                                      <  1:3112915/150‑1 (MQ=255)
             gCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACa                                                                                                                                    <  2:1627452/150‑1 (MQ=255)
              cACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACaa                                                                                                                                   <  1:714004/150‑1 (MQ=255)
              cACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACaa                                                                                                                                   <  1:4313502/150‑1 (MQ=255)
              cACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACaa                                                                                                                                   >  2:3873421/1‑150 (MQ=255)
               aCCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACaaa                                                                                                                                  >  2:4054584/1‑150 (MQ=255)
                         aTTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCga                                                                                                                        >  2:741965/1‑150 (MQ=255)
                          ttCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCgaa                                                                                                                       <  2:5176047/150‑1 (MQ=255)
                          ttCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCgaa                                                                                                                       >  1:1349849/1‑150 (MQ=255)
                            ccGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCgaaga                                                                                                                     <  1:4327431/150‑1 (MQ=255)
                              gACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATATACAAAGCCATCGCGAAGaaa                                                                                                                   <  2:3971930/150‑1 (MQ=255)
                                 atcatTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATtc                                                                                                                <  1:4974921/150‑1 (MQ=255)
                                  tcatTTTGCCGCCGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATTTGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATtct                                                                                                               <  1:882062/150‑1 (MQ=255)
                                   catTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATtctc                                                                                                              >  2:3478831/1‑150 (MQ=255)
                                    atTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTcc                                                                                                             >  2:4728553/1‑150 (MQ=255)
                                    atTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTcc                                                                                                             >  1:4453941/1‑150 (MQ=255)
                                      tttGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGa                                                                                                           >  2:146487/1‑150 (MQ=255)
                                      tttGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGa                                                                                                           >  2:3104400/1‑150 (MQ=255)
                                        tGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAg                                                                                                         <  1:2125767/150‑1 (MQ=255)
                                          ccGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAgtg                                                                                                       <  2:707178/150‑1 (MQ=255)
                                          ccGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAgtg                                                                                                       <  1:3601042/150‑1 (MQ=255)
                                           cGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAgtgt                                                                                                      >  1:4720884/1‑150 (MQ=255)
                                            gACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGtgtg                                                                                                     >  2:2734672/1‑150 (MQ=255)
                                                  gACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCgg                                                                                               >  1:2434590/1‑150 (MQ=255)
                                                     ctggaccGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCa                                                                                            <  1:4900676/144‑1 (MQ=255)
                                                     cTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCa                                                                                            >  1:4797222/1‑150 (MQ=255)
                                                     cTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCa                                                                                            <  1:3921473/150‑1 (MQ=255)
                                                        gTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCt                                                                                         <  1:4425370/150‑1 (MQ=255)
                                                         tACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTg                                                                                        >  2:527306/1‑150 (MQ=255)
                                                         tACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTGTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTg                                                                                        <  2:5014602/150‑1 (MQ=255)
                                                          aCGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTgg                                                                                       <  2:1970847/150‑1 (MQ=255)
                                                           cGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTggg                                                                                      >  2:3581962/1‑150 (MQ=255)
                                                           cGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTggg                                                                                      <  1:2067801/150‑1 (MQ=255)
                                                             aaaaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTa                                                                                    >  1:478192/1‑150 (MQ=255)
                                                             aaaaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTa                                                                                    >  1:5096592/1‑150 (MQ=255)
                                                             aaaaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTa                                                                                    >  1:478170/1‑150 (MQ=255)
                                                              aaaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTaa                                                                                   >  2:1093051/1‑150 (MQ=255)
                                                              aaaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTaa                                                                                   >  2:2288525/1‑150 (MQ=255)
                                                              aaaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTaa                                                                                   >  2:428056/1‑150 (MQ=255)
                                                                   acgacATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGaaa                                                                              <  1:58547/150‑1 (MQ=255)
                                                                      acATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCg                                                                           >  1:366783/1‑150 (MQ=255)
                                                                       cATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGa                                                                          <  2:2168464/150‑1 (MQ=255)
                                                                        aTGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGAc                                                                         <  1:2221997/150‑1 (MQ=255)
                                                                          gAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACAt                                                                       >  1:2900271/1‑150 (MQ=255)
                                                                           aaTACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATc                                                                      >  1:1832872/1‑150 (MQ=255)
                                                                            aTACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCTAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCg                                                                     <  1:4539760/150‑1 (MQ=255)
                                                                            aTACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCg                                                                     <  2:181898/150‑1 (MQ=255)
                                                                              aCTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTc                                                                   >  1:4940176/1‑150 (MQ=255)
                                                                                   ggATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTCAAGAAACCGACATCGTCACGCt                                                              >  2:2765490/1‑150 (MQ=255)
                                                                                   ggATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCt                                                              <  2:1321329/150‑1 (MQ=255)
                                                                                    gATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTg                                                             >  2:1185379/1‑150 (MQ=255)
                                                                                     atatGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGc                                                            >  2:1518269/1‑150 (MQ=255)
                                                                                      tatGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGcc                                                           >  2:2503546/1‑150 (MQ=255)
                                                                                        tGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa                                                         <  1:1184258/150‑1 (MQ=255)
                                                                                        tGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa                                                         >  2:2606186/1‑150 (MQ=255)
                                                                                        tGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa                                                         >  1:3393956/1‑150 (MQ=255)
                                                                                        tGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa                                                         >  1:1459776/1‑150 (MQ=255)
                                                                                        tGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa                                                         <  1:3402862/150‑1 (MQ=255)
                                                                                         gCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTAt                                                        <  2:5149081/150‑1 (MQ=255)
                                                                                         gCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTAt                                                        >  1:2532809/1‑150 (MQ=255)
                                                                                         gCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTAt                                                        >  2:2887047/1‑150 (MQ=255)
                                                                                          cATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATc                                                       >  2:2098740/1‑150 (MQ=255)
                                                                                           aTCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATcc                                                      <  1:4733923/150‑1 (MQ=255)
                                                                                            tCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCa                                                     <  1:2625918/150‑1 (MQ=255)
                                                                                             ccGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAg                                                    >  1:1151572/1‑150 (MQ=255)
                                                                                               gTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCa                                                  >  1:4860174/1‑150 (MQ=255)
                                                                                                    ttCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGAct                                             >  2:2013098/1‑150 (MQ=255)
                                                                                                         ccGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGcc                                        >  2:242124/1‑150 (MQ=255)
                                                                                                            cggTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCt                                     <  2:4418942/148‑1 (MQ=255)
                                                                                                              gTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGa                                   <  1:1676730/150‑1 (MQ=255)
                                                                                                                     gcggTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGgcg                            >  2:4097040/1‑150 (MQ=255)
                                                                                                                          cGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGcc                       >  2:4130185/1‑150 (MQ=255)
                                                                                                                           gATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCg                      >  1:521616/1‑150 (MQ=255)
                                                                                                                            aTCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGc                     >  2:3815926/1‑150 (MQ=255)
                                                                                                                             tCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCt                    <  2:1016864/150‑1 (MQ=255)
                                                                                                                               cGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTgg                  >  1:1740739/1‑150 (MQ=255)
                                                                                                                                   cTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGAtgt              >  2:323571/1‑150 (MQ=255)
                                                                                                                                   cTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGAtgt              >  1:2382449/1‑150 (MQ=255)
                                                                                                                                    tGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGAtgtg             >  2:4481541/1‑150 (MQ=255)
                                                                                                                                    tGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGAtgtg             >  2:1411276/1‑150 (MQ=255)
                                                                                                                                     gggAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGa            <  2:1349849/150‑1 (MQ=255)
                                                                                                                                        aaGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAg         <  2:5215158/150‑1 (MQ=255)
                                                                                                                                        aaGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAg         <  1:2780951/150‑1 (MQ=255)
                                                                                                                                          gCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGAc       >  1:5264677/1‑150 (MQ=255)
                                                                                                                                           cGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACt      <  2:547236/150‑1 (MQ=255)
                                                                                                                                           cGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACt      >  2:900046/1‑150 (MQ=255)
                                                                                                                                             aaaaGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATTGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTgg    >  2:614190/1‑150 (MQ=255)
                                                                                                                                              aaaGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGa   >  2:3932841/1‑150 (MQ=255)
                                                                                                                                               aaGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGaa  <  2:4797222/150‑1 (MQ=255)
                                                                                                                                               aaGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGaa  <  2:4361754/150‑1 (MQ=255)
                                                                                                                                               aaGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGaa  >  1:4667683/1‑150 (MQ=255)
                                                                                                                                               aaGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGaa  <  2:2565572/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAA  >  CP009273/1277639‑1277931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGAC‑GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAA  >  CP009273/1277638‑1277934
                                                                                                                                                     |                                                                                                                                                    
CTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGAC‑GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA                                                                                                                                                     <  SRR19973062.3003762/150‑1 (MQ=60)
CTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGAC‑GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA                                                                                                                                                     >  SRR19973062.2926984/1‑150 (MQ=60)
CTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGAC‑GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAA                                                                                                                                                     >  SRR19973062.1671153/1‑150 (MQ=60)
 TTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGAC‑GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAAC                                                                                                                                                    <  SRR19973062.3859956/150‑1 (MQ=60)
     GTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGAC‑GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGG                                                                                                                                                <  SRR19973062.408995/150‑1 (MQ=60)
     GTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGAC‑GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGG                                                                                                                                                <  SRR19973062.408901/150‑1 (MQ=60)
     GTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGAC‑GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGG                                                                                                                                                <  SRR19973062.408869/150‑1 (MQ=60)
      TCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGAC‑GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGA                                                                                                                                               >  SRR19973062.2579563/1‑150 (MQ=60)
        TGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGAC‑GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAA                                                                                                                                             >  SRR19973062.4019697/1‑150 (MQ=60)
         GTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGAC‑GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAAT                                                                                                                                            <  SRR19973062.818573/150‑1 (MQ=60)
         GTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGAC‑GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAAT                                                                                                                                            <  SRR19973062.4449290/150‑1 (MQ=60)
           CGAGCACCTTTCTCTATTCCGACATAATTTTGCCGAC‑GGCGACCTGGTACGAAAAAGACGACATGATTACTTCGGATATGAATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCT                                                                                                                                          <  SRR19973062.2574268/150‑1 (MQ=60)
            GAGCACCTGTCTCTATTCCGACATCATTTTGCCGAC‑GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGACAAGCAACTGGGAAATCTA                                                                                                                                         >  SRR19973062.4116300/1‑150 (MQ=60)
            GAGCACCTGTCTCTATTCCGACATCATTTTGCCGAC‑GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTA                                                                                                                                         <  SRR19973062.3128942/150‑1 (MQ=60)
            GAGCACCTGTCTCTATTCCGACATCATTTTGCCGAC‑GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTA                                                                                                                                         <  SRR19973062.421153/150‑1 (MQ=60)
               aacgtgtataTATTCCGACATATTTTTGCCGAC‑GGCGACCTGGTACTAAAAAGACGACATGAATACTTCGGATTTGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGTACGCTAAAAGCAACTGGGAAATCTACAA                                                                                                                                      <  SRR19973062.4926886/140‑1 (MQ=60)
               CACCTGTCTTTATTCCTACAT‑ATTTTGGCGGCGGGCGACCTGTAACTAAAAAGACGACATGAATAATTGGTATTTGAATCGTTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCGGGGAAGCTAAAAGCAACTGGGAAATCTACAA                                                                                                                                      <  SRR19973062.4926472/150‑1 (MQ=35)
               CACCTGTCTCTATTCCGACATCATTTTGCCGAC‑GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAA                                                                                                                                      <  SRR19973062.1839713/150‑1 (MQ=60)
               CACCTGTCTCTATTCCGACATCATTTTGCCGAC‑GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAA                                                                                                                                      >  SRR19973062.5176297/1‑150 (MQ=60)
               CACCTGTCTCTATTCCGACATCATTTTGCCGAC‑GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAA                                                                                                                                      <  SRR19973062.717279/150‑1 (MQ=60)
               CACCTGTCTCTATTCCGACATCATTTTGCCGAC‑GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAA                                                                                                                                      <  SRR19973062.4335744/150‑1 (MQ=60)
                 CCTGTCTCTATTCCGACATCATTTTGCCGAC‑GGCGACCTGGTACGAAAAATACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAG                                                                                                                                    <  SRR19973062.5004787/150‑1 (MQ=60)
                   ttttTCTATTCCTACATCTTTTTTCCGTC‑GTCGACCTGGTACGAAAATGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGATGTCTGCGGCGGTCTTTCCGGCATGGGATGCGAAAAGCAACTGGGAAATCTACAAAGCC                                                                                                                                  <  SRR19973062.476348/146‑1 (MQ=60)
                     TATCTATTCCGACATCATTTTGCCGAC‑GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCAT                                                                                                                                <  SRR19973062.4039874/150‑1 (MQ=60)
                      CTCTATTCCGACATCATTTTGCCGAC‑GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATC                                                                                                                               >  SRR19973062.2108119/1‑150 (MQ=60)
                           TTCCGACATCATTTTGCCGAC‑GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAA                                                                                                                          >  SRR19973062.1355946/1‑150 (MQ=60)
                             ccgccctcctcTTGCCGAC‑GGCGACCCGGTACGAAACAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGA                                                                                                                        <  SRR19973062.4349672/139‑1 (MQ=60)
                             CCGACATCATTTTGCCGAC‑GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGA                                                                                                                        <  SRR19973062.4349724/150‑1 (MQ=60)
                              CGACATCATTTTGCCGAC‑GGCGACCTGGTACTAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAA                                                                                                                       <  SRR19973062.2296125/150‑1 (MQ=60)
                              CGACATCATTTTGCCGAC‑GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAA                                                                                                                       >  SRR19973062.3413665/1‑150 (MQ=60)
                                 CATCATTTTGCCGAC‑GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATT                                                                                                                    <  SRR19973062.673537/150‑1 (MQ=60)
                                  ATCATTTTGCCGAC‑GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTC                                                                                                                   <  SRR19973062.5001147/150‑1 (MQ=60)
                                   TCATTTTGCCGCC‑GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATTTGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCT                                                                                                                  <  SRR19973062.885842/150‑1 (MQ=60)
                                   TCATTTTGCCGAC‑GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCT                                                                                                                  <  SRR19973062.4814049/150‑1 (MQ=60)
                                   TCATTTTGCCGAC‑GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCT                                                                                                                  <  SRR19973062.4814022/150‑1 (MQ=60)
                                   TCATTTTGCCGAC‑GGCGACCGGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCT                                                                                                                  <  SRR19973062.4183825/150‑1 (MQ=60)
                                     ATTTTGCCGAC‑GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCC                                                                                                                >  SRR19973062.4476640/1‑150 (MQ=60)
                                      TTTTGCCGAC‑GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCG                                                                                                               <  SRR19973062.3439581/150‑1 (MQ=60)
                                         TGCCGAC‑GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAG                                                                                                            <  SRR19973062.528661/150‑1 (MQ=60)
                                         TGCCGAC‑GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAG                                                                                                            <  SRR19973062.2136570/150‑1 (MQ=60)
                                           CCGAC‑GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTG                                                                                                          <  SRR19973062.3619349/150‑1 (MQ=60)
                                            CGAC‑GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGT                                                                                                         >  SRR19973062.4745523/1‑150 (MQ=60)
                                             GAC‑GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTG                                                                                                        >  SRR19973062.1661514/1‑150 (MQ=60)
                                               C‑GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCG                                                                                                      >  SRR19973062.1696435/1‑150 (MQ=60)
                                                  GCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTC                                                                                                    <  SRR19973062.3902025/150‑1 (MQ=60)
                                                   CGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCG                                                                                                   <  SRR19973062.3658527/150‑1 (MQ=60)
                                                   CGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCG                                                                                                   <  SRR19973062.3658677/150‑1 (MQ=60)
                                                    GACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGG                                                                                                  >  SRR19973062.2447658/1‑150 (MQ=60)
                                                    GACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGG                                                                                                  >  SRR19973062.2447644/1‑150 (MQ=60)
                                                       CTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCA                                                                                               >  SRR19973062.4822108/1‑150 (MQ=60)
                                                       CTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCA                                                                                               <  SRR19973062.3941422/150‑1 (MQ=60)
                                                       CTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCA                                                                                               >  SRR19973062.4384148/1‑150 (MQ=60)
                                                       CTGGACCGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCA                                                                                               <  SRR19973062.4925929/150‑1 (MQ=60)
                                                        TGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCAT                                                                                              >  SRR19973062.4504635/1‑150 (MQ=60)
                                                        TGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCAT                                                                                              >  SRR19973062.4504214/1‑150 (MQ=60)
                                                         GGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATACTCCGAAGTGTGCGTCGGCCATC                                                                                             >  SRR19973062.4912480/1‑150 (MQ=60)
                                                          GTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCT                                                                                            <  SRR19973062.4447972/150‑1 (MQ=60)
                                                            ACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGG                                                                                          <  SRR19973062.4842208/150‑1 (MQ=60)
                                                             CGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGG                                                                                         <  SRR19973062.2078386/150‑1 (MQ=60)
                                                             CGAAAAAGACGACATGAAGACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGG                                                                                         <  SRR19973062.2095209/150‑1 (MQ=60)
                                                               AAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTA                                                                                       >  SRR19973062.480363/1‑150 (MQ=60)
                                                               AAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTA                                                                                       >  SRR19973062.480344/1‑150 (MQ=60)
                                                               AAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTA                                                                                       >  SRR19973062.480341/1‑150 (MQ=60)
                                                               AAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTA                                                                                       >  SRR19973062.5123570/1‑150 (MQ=60)
                                                                 AAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGTGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAA                                                                                     >  SRR19973062.2768799/1‑150 (MQ=60)
                                                                  AAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAG                                                                                    >  SRR19973062.4855998/1‑150 (MQ=60)
                                                                  AAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAG                                                                                    <  SRR19973062.201779/150‑1 (MQ=60)
                                                                  AAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAG                                                                                    <  SRR19973062.4922577/150‑1 (MQ=60)
                                                                     ACGACATGAATACTTCGGATATGCATCGGTTTTTTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAA                                                                                 <  SRR19973062.2328626/150‑1 (MQ=60)
                                                                     ACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAA                                                                                 <  SRR19973062.58955/150‑1 (MQ=60)
                                                                        ACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCG                                                                              >  SRR19973062.368434/1‑150 (MQ=60)
                                                                         CATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGA                                                                             >  SRR19973062.2963340/1‑150 (MQ=60)
                                                                          ATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGAC                                                                            <  SRR19973062.2233125/150‑1 (MQ=60)
                                                                           TGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACA                                                                           <  SRR19973062.4326867/150‑1 (MQ=60)
                                                                           TGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACA                                                                           >  SRR19973062.4752194/1‑150 (MQ=60)
                                                                            GAATACTTCGGTTTTGCATCCGTTTATTCACCCGCTGTCTGGGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACAT                                                                          <  SRR19973062.4979007/150‑1 (MQ=60)
                                                                            GAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACAT                                                                          >  SRR19973062.2915578/1‑150 (MQ=60)
                                                                             AATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATC                                                                         <  SRR19973062.1398415/150‑1 (MQ=60)
                                                                             AATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATC                                                                         >  SRR19973062.1841948/1‑150 (MQ=60)
                                                                              ATACTTCGTATTTTCATCAGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGTAATGGTAAGCGAAAATCAACTGGGAAATCTACAAAGCCATCGAGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCG                                                                        <  SRR19973062.4563420/150‑1 (MQ=60)
                                                                              ATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCTAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCG                                                                        <  SRR19973062.4563417/150‑1 (MQ=60)
                                                                                ACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTC                                                                      >  SRR19973062.4966027/1‑150 (MQ=60)
                                                                                   TCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACG                                                                   <  SRR19973062.3266241/150‑1 (MQ=60)
                                                                                     GGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCT                                                                 >  SRR19973062.4737247/1‑150 (MQ=60)
                                                                                      TATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTG                                                                <  SRR19973062.218490/150‑1 (MQ=60)
                                                                                      GATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTG                                                                <  SRR19973062.1431486/150‑1 (MQ=60)
                                                                                      GATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTG                                                                <  SRR19973062.6963/150‑1 (MQ=60)
                                                                                      GATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATACTCCGAAGTGTGCGTCGGCCATCTGGGTCAAGAAACCGACATCGTCACGCTG                                                                >  SRR19973062.4345669/1‑150 (MQ=60)
                                                                                       ATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGC                                                               >  SRR19973062.4192548/1‑150 (MQ=60)
                                                                                         ATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCT                                                             >  SRR19973062.4314165/1‑150 (MQ=60)
                                                                                          TGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTA                                                            >  SRR19973062.1466913/1‑150 (MQ=60)
                                                                                          TGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTA                                                            >  SRR19973062.3411373/1‑150 (MQ=60)
                                                                                          TGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTA                                                            <  SRR19973062.2850618/150‑1 (MQ=60)
                                                                                          TGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTA                                                            <  SRR19973062.1189801/150‑1 (MQ=60)
                                                                                          TGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTA                                                            <  SRR19973062.3420303/150‑1 (MQ=60)
                                                                                           GCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTAT                                                           >  SRR19973062.2546323/1‑150 (MQ=60)
                                                                                            CATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATC                                                          >  SRR19973062.3097456/1‑150 (MQ=60)
                                                                                            CATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACAACGTCACGCTGCCTATC                                                          >  SRR19973062.401240/1‑150 (MQ=60)
                                                                                             ATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCC                                                         <  SRR19973062.4758606/150‑1 (MQ=60)
                                                                                              TCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCA                                                        <  SRR19973062.2639759/150‑1 (MQ=60)
                                                                                               CCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAG                                                       >  SRR19973062.1156964/1‑150 (MQ=60)
                                                                                                 GTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCA                                                     >  SRR19973062.521305/1‑150 (MQ=60)
                                                                                                 GTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCA                                                     >  SRR19973062.4885242/1‑150 (MQ=60)
                                                                                                     ATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGAC                                                 >  SRR19973062.4727427/1‑150 (MQ=60)
                                                                                                     ATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGAC                                                 >  SRR19973062.2835546/1‑150 (MQ=60)
                                                                                                       TCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAGATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTC                                               >  SRR19973062.5040272/1‑150 (MQ=60)
                                                                                                        CACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACAGGGAAATCTACAAAGCCATCGCGAAGAAATACTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACAACGTCACGCAGCCTATCCAGCATGACTCA                                              >  SRR19973062.4384619/1‑150 (MQ=60)
                                                                                                               TGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTG                                       <  SRR19973062.5259223/150‑1 (MQ=60)
                                                                                                                GTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGA                                      <  SRR19973062.1684967/150‑1 (MQ=60)
                                                                                                                GTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGA                                      >  SRR19973062.2534903/1‑150 (MQ=60)
                                                                                                                   TGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACT                                   <  SRR19973062.540828/150‑1 (MQ=60)
                                                                                                                    GCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTG                                  >  SRR19973062.1178598/1‑150 (MQ=60)
                                                                                                                      GGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGC                                <  SRR19973062.2474117/150‑1 (MQ=60)
                                                                                                                        CGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGC                              >  SRR19973062.965578/1‑150 (MQ=60)
                                                                                                                         GGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAATAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCA                             >  SRR19973062.891524/1‑150 (MQ=60)
                                                                                                                         GGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCA                             >  SRR19973062.4458761/1‑150 (MQ=60)
                                                                                                                          GTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAG                            >  SRR19973062.3144912/1‑150 (MQ=60)
                                                                                                                             GATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCG                         >  SRR19973062.524078/1‑150 (MQ=60)
                                                                                                                             GATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCAATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCG                         >  SRR19973062.4087642/1‑150 (MQ=60)
                                                                                                                               TCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCT                       <  SRR19973062.150098/150‑1 (MQ=60)
                                                                                                                               TCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCT                       >  SRR19973062.34638/1‑150 (MQ=60)
                                                                                                                               TCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCT                       >  SRR19973062.1255887/1‑150 (MQ=60)
                                                                                                                               TCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCT                       <  SRR19973062.4954195/150‑1 (MQ=60)
                                                                                                                                 CGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGG                     >  SRR19973062.1749202/1‑150 (MQ=60)
                                                                                                                                    CCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATG                  >  SRR19973062.4748667/1‑150 (MQ=60)
                                                                                                                                    CCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATG                  >  SRR19973062.2055168/1‑150 (MQ=60)
                                                                                                                                     CTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGT                 >  SRR19973062.2395219/1‑150 (MQ=60)
                                                                                                                                          AAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAG            <  SRR19973062.2795642/150‑1 (MQ=60)
                                                                                                                                            GCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGAC          >  SRR19973062.5292378/1‑150 (MQ=60)
                                                                                                                                             CGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTCAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACT         >  SRR19973062.74272/1‑150 (MQ=60)
                                                                                                                                              GAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTG        >  SRR19973062.179161/1‑150 (MQ=60)
                                                                                                                                              GAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTG        <  SRR19973062.4776317/150‑1 (MQ=60)
                                                                                                                                                 AAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAA     >  SRR19973062.2425172/1‑150 (MQ=60)
                                                                                                                                                 AAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAA     <  SRR19973062.1337674/150‑1 (MQ=60)
                                                                                                                                                 AAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAA     >  SRR19973062.4692099/1‑150 (MQ=60)
                                                                                                                                                    CAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAA  >  SRR19973062.2527265/1‑150 (MQ=60)
                                                                                                                                                    CAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAA  >  SRR19973062.4307825/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
CTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGAC‑GGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAA  >  CP009273/1277638‑1277934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑