Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,519,349 T→A 100% K297* (AAA→TAA)  ansP ← L‑asparagine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,519,3490TA97.2% 856.3 / 9.6 248K297* (AAA→TAA) ansPL‑asparagine transporter
Reads supporting (aligned to +/- strand):  ref base T (3/4);  new base A (114/127);  total (117/131)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 253 total aligned reads displayed.

TTTTCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTT  >  CP009273/1519202‑1519498
                                                                                                                                                   |                                                                                                                                                     
ttttCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAag                                                                                                                                                     >  1:2139241/1‑150 (MQ=255)
 tttCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAaga                                                                                                                                                    <  2:4404584/150‑1 (MQ=255)
  ttCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAagag                                                                                                                                                   <  1:155285/150‑1 (MQ=255)
   tCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAgaga                                                                                                                                                  <  1:1872172/150‑1 (MQ=255)
    cGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGaa                                                                                                                                                 <  2:169904/150‑1 (MQ=255)
     gCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGaaa                                                                                                                                                >  2:3437967/1‑150 (MQ=255)
     gCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGaaa                                                                                                                                                <  1:2335576/150‑1 (MQ=255)
      ccATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGaaaa                                                                                                                                               >  1:2373080/1‑150 (MQ=255)
      ccATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGaaaa                                                                                                                                               >  1:2373280/1‑150 (MQ=255)
         tAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACg                                                                                                                                            >  2:4118278/1‑150 (MQ=255)
            aaCTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCa                                                                                                                                         <  2:4300728/150‑1 (MQ=255)
             aCTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCAc                                                                                                                                        <  2:9494/150‑1 (MQ=255)
                cGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGaa                                                                                                                                     >  1:4310518/1‑150 (MQ=255)
                 ggTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAAc                                                                                                                                    >  1:359792/1‑150 (MQ=255)
                  gTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACg                                                                                                                                   <  1:4362300/150‑1 (MQ=255)
                    gCGGAACCGCCCATCGCCTTTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCTGGCTGGAGAGGGCAGCTGTCAGCACCACAATTTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGa                                                                                                                                 <  1:4031523/150‑1 (MQ=255)
                    gCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGa                                                                                                                                 >  2:4548943/1‑150 (MQ=255)
                        aaCCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACttt                                                                                                                             <  1:4095263/150‑1 (MQ=255)
                        aaCCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACttt                                                                                                                             <  2:3082327/150‑1 (MQ=255)
                         aCCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTg                                                                                                                            >  2:3950013/1‑150 (MQ=255)
                          ccGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGc                                                                                                                           >  2:921436/1‑150 (MQ=255)
                              ccATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCg                                                                                                                       >  2:3531221/1‑150 (MQ=255)
                                  cGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTg                                                                                                                   >  1:3506603/1‑150 (MQ=255)
                                   gCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGa                                                                                                                  >  2:2106246/1‑150 (MQ=255)
                                   gCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGa                                                                                                                  <  1:1008823/150‑1 (MQ=255)
                                             gcAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTcc                                                                                                        >  1:1471446/1‑150 (MQ=255)
                                               aGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCAc                                                                                                      <  2:944369/150‑1 (MQ=255)
                                                       tCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAataa                                                                                              <  2:2198641/150‑1 (MQ=255)
                                                       tCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAataa                                                                                              >  2:3394474/1‑150 (MQ=255)
                                                       tCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAataa                                                                                              >  1:1295029/1‑150 (MQ=255)
                                                            tGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAataacataa                                                                                         >  2:4038766/1‑150 (MQ=255)
                                                             gCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAataacataac                                                                                        <  2:2264290/150‑1 (MQ=255)
                                                             gCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAataacataac                                                                                        >  1:2158931/1‑150 (MQ=255)
                                                              cAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAAcc                                                                                       >  2:714299/1‑150 (MQ=255)
                                                              cAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAAcc                                                                                       >  2:681643/1‑150 (MQ=255)
                                                               aGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCa                                                                                      >  2:3974111/1‑150 (MQ=255)
                                                               aGTACAGACCTGAATTCAGGCTGGAGAGGGAAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCa                                                                                      >  1:4458361/1‑150 (MQ=255)
                                                                  aCAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCa                                                                                   <  2:3126880/150‑1 (MQ=255)
                                                                      aCCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAacac                                                                               <  1:19740/150‑1 (MQ=255)
                                                                        cTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAAcacca                                                                             >  2:1821750/1‑150 (MQ=255)
                                                                           aaTTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACgg                                                                          <  2:635187/150‑1 (MQ=255)
                                                                             ttCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAg                                                                        <  1:9211/150‑1 (MQ=255)
                                                                             ttCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAg                                                                        <  1:3918946/150‑1 (MQ=255)
                                                                               cAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGcc                                                                      >  1:263295/1‑150 (MQ=255)
                                                                                 ggCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGa                                                                    <  1:2783195/150‑1 (MQ=255)
                                                                                   cTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACg                                                                  <  1:2319788/150‑1 (MQ=255)
                                                                                   cTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACg                                                                  <  2:1320722/150‑1 (MQ=255)
                                                                                    tGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGt                                                                 <  2:2732052/150‑1 (MQ=255)
                                                                                       agagGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTaaa                                                              >  2:2403471/1‑150 (MQ=255)
                                                                                       agagGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTaaa                                                              <  1:3003117/150‑1 (MQ=255)
                                                                                       agagGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTaaa                                                              <  2:3232641/150‑1 (MQ=255)
                                                                                        gagGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTaaaa                                                             <  1:245898/150‑1 (MQ=255)
                                                                                         agGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAAc                                                            <  1:1422168/150‑1 (MQ=255)
                                                                                         agGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAAc                                                            <  1:3220181/150‑1 (MQ=255)
                                                                                           ggCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAg                                                          <  1:4623570/150‑1 (MQ=255)
                                                                                              aGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGcc                                                       >  1:581503/1‑150 (MQ=255)
                                                                                              aGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGcc                                                       >  1:1734632/1‑150 (MQ=255)
                                                                                               gCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCa                                                      <  1:4179556/150‑1 (MQ=255)
                                                                                                 ggTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAAt                                                    >  2:2912227/1‑150 (MQ=255)
                                                                                                 ggTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAAt                                                    >  1:4589045/1‑150 (MQ=255)
                                                                                                   tCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATAc                                                  <  1:2064324/150‑1 (MQ=255)
                                                                                                     aGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGc                                                >  1:3254781/1‑150 (MQ=255)
                                                                                                      gCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGcc                                               >  2:3275901/1‑150 (MQ=255)
                                                                                                      gCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGcc                                               >  1:537190/1‑150 (MQ=255)
                                                                                                         ccacAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCaaa                                            >  2:3503633/1‑150 (MQ=255)
                                                                                                         ccacAATGTTCATAAAGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCaaa                                            >  2:1784794/1‑150 (MQ=255)
                                                                                                          cacaATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAAt                                           <  2:997092/150‑1 (MQ=255)
                                                                                                               tGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCacac                                      <  2:2683852/150‑1 (MQ=255)
                                                                                                                gTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACt                                     >  1:716626/1‑150 (MQ=255)
                                                                                                                   cATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGtt                                  >  2:1976324/1‑150 (MQ=255)
                                                                                                                   cATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGtt                                  >  1:353109/1‑150 (MQ=255)
                                                                                                                    aTAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTa                                 >  2:4562103/1‑150 (MQ=255)
                                                                                                                     tAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTaa                                <  1:4409490/150‑1 (MQ=255)
                                                                                                                      aaTGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAAt                               <  1:3333645/150‑1 (MQ=255)
                                                                                                                       aTGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATg                              >  1:1124147/1‑150 (MQ=255)
                                                                                                                       aTGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATg                              <  2:710687/150‑1 (MQ=255)
                                                                                                                        tgctgcCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATgg                             <  2:3071971/150‑1 (MQ=255)
                                                                                                                          ctgcCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCt                           >  1:2963391/1‑150 (MQ=255)
                                                                                                                            gcCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCttt                         <  1:2709069/150‑1 (MQ=255)
                                                                                                                             ccGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTa                        <  2:1692819/150‑1 (MQ=255)
                                                                                                                              cGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAg                       <  1:2620914/150‑1 (MQ=255)
                                                                                                                               gATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAgg                      <  1:2803428/150‑1 (MQ=255)
                                                                                                                               gATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAgg                      <  1:253259/150‑1 (MQ=255)
                                                                                                                                atatatGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGc                     <  1:3974111/150‑1 (MQ=255)
                                                                                                                                atatatGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGc                     <  2:4183981/150‑1 (MQ=255)
                                                                                                                                atatatGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGc                     <  1:4221154/150‑1 (MQ=255)
                                                                                                                                  atatGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCAc                   <  2:1867954/150‑1 (MQ=255)
                                                                                                                                   tatGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCAcc                  <  2:2526314/150‑1 (MQ=255)
                                                                                                                                    atGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCa                 <  1:2886396/150‑1 (MQ=255)
                                                                                                                                     tGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCAt                <  2:3709000/150‑1 (MQ=255)
                                                                                                                                      ggCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATg               >  2:4617253/1‑150 (MQ=255)
                                                                                                                                        cacaCCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGt             <  2:4161404/150‑1 (MQ=255)
                                                                                                                                        cacaCCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGt             >  2:3984483/1‑150 (MQ=255)
                                                                                                                                        cacaCCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGt             >  2:744902/1‑150 (MQ=255)
                                                                                                                                          cacCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCt           <  1:3768734/150‑1 (MQ=255)
                                                                                                                                           acCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTg          <  1:1650839/150‑1 (MQ=255)
                                                                                                                                            cccAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCCCGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGc         <  2:4642977/150‑1 (MQ=255)
                                                                                                                                              cAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCgg       >  1:22609/1‑150 (MQ=255)
                                                                                                                                               aGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGa      >  2:1883603/1‑150 (MQ=255)
                                                                                                                                                 tttAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATc    <  1:1085462/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TTTTCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTT  >  CP009273/1519202‑1519498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTTTCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTT  >  CP009273/1519201‑1519498
                                                                                                                                                    |                                                                                                                                                     
ATTTTCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAA                                                                                                                                                      >  SRR19973072.3263067/1‑150 (MQ=60)
ATTTTCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAA                                                                                                                                                      <  SRR19973072.1540544/150‑1 (MQ=60)
ATTTTCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAA                                                                                                                                                      <  SRR19973072.1540404/150‑1 (MQ=60)
ATTTTCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAA                                                                                                                                                      >  SRR19973072.3806519/1‑150 (MQ=60)
 TTTTCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAG                                                                                                                                                     >  SRR19973072.2143986/1‑150 (MQ=60)
 TTTTCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAG                                                                                                                                                     >  SRR19973072.2143904/1‑150 (MQ=60)
   TTCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAG                                                                                                                                                   <  SRR19973072.155696/150‑1 (MQ=60)
   TTCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAG                                                                                                                                                   <  SRR19973072.155681/150‑1 (MQ=60)
    TCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGT                                                                                                                                                  <  SRR19973072.775659/150‑1 (MQ=60)
    TCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGA                                                                                                                                                  <  SRR19973072.1876198/150‑1 (MQ=60)
    TCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGA                                                                                                                                                  <  SRR19973072.2714327/150‑1 (MQ=60)
     CGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAA                                                                                                                                                 >  SRR19973072.4170181/1‑150 (MQ=60)
     CGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAA                                                                                                                                                 >  SRR19973072.4454388/1‑150 (MQ=60)
     CGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAA                                                                                                                                                 >  SRR19973072.4170125/1‑150 (MQ=60)
      GCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAA                                                                                                                                                >  SRR19973072.1396229/1‑150 (MQ=60)
      GCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAA                                                                                                                                                <  SRR19973072.2340585/150‑1 (MQ=60)
       CCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAA                                                                                                                                               >  SRR19973072.4003908/1‑150 (MQ=60)
       CCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAA                                                                                                                                               >  SRR19973072.2378184/1‑150 (MQ=60)
       CCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAA                                                                                                                                               >  SRR19973072.2378204/1‑150 (MQ=60)
       CCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAA                                                                                                                                               >  SRR19973072.2378376/1‑150 (MQ=60)
       CCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAA                                                                                                                                               >  SRR19973072.2378404/1‑150 (MQ=60)
          TAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACG                                                                                                                                            >  SRR19973072.2689572/1‑150 (MQ=60)
             AACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCA                                                                                                                                         <  SRR19973072.2333581/150‑1 (MQ=60)
                 CGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAA                                                                                                                                     >  SRR19973072.2352113/1‑150 (MQ=60)
                 CGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAA                                                                                                                                     >  SRR19973072.4319525/1‑150 (MQ=60)
                  GGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAAC                                                                                                                                    >  SRR19973072.360588/1‑150 (MQ=60)
                   GTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACG                                                                                                                                   <  SRR19973072.4371465/150‑1 (MQ=60)
                    TGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGG                                                                                                                                  >  SRR19973072.1055125/1‑150 (MQ=60)
                     GCGGAACCGCCCTTCGCCTTTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCTGGCTGGAGAGGGCAGCTGTCAGCACCACAATTTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGA                                                                                                                                 <  SRR19973072.4039992/150‑1 (MQ=60)
                       GGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACT                                                                                                                               >  SRR19973072.22737/1‑150 (MQ=60)
                       GGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACT                                                                                                                               <  SRR19973072.441910/150‑1 (MQ=60)
                         AACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTT                                                                                                                             <  SRR19973072.4103864/150‑1 (MQ=60)
                          ACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTG                                                                                                                            >  SRR19973072.3330505/1‑150 (MQ=60)
                                   CGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTG                                                                                                                   <  SRR19973072.3372170/150‑1 (MQ=60)
                                   CGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTG                                                                                                                   >  SRR19973072.3513955/1‑150 (MQ=60)
                                    GCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGA                                                                                                                  <  SRR19973072.1011021/150‑1 (MQ=60)
                                      CATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATA                                                                                                                >  SRR19973072.4468928/1‑150 (MQ=60)
                                      CATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATA                                                                                                                <  SRR19973072.47430/150‑1 (MQ=60)
                                       ATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATAC                                                                                                               >  SRR19973072.2935744/1‑150 (MQ=60)
                                        TTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACG                                                                                                              <  SRR19973072.1416422/150‑1 (MQ=60)
                                              GCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCC                                                                                                        >  SRR19973072.1474698/1‑150 (MQ=60)
                                              GCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCC                                                                                                        >  SRR19973072.1474629/1‑150 (MQ=60)
                                              GCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCC                                                                                                        >  SRR19973072.1474600/1‑150 (MQ=60)
                                                AGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCATTAAAGAGAAAAACGACACAAACGAACTTTGCCCCGCATGAAACCCGCTCCAC                                                                                                      >  SRR19973072.1427181/1‑150 (MQ=60)
                                                 GAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACG                                                                                                     <  SRR19973072.4013042/150‑1 (MQ=60)
                                                        TCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAA                                                                                              >  SRR19973072.1297833/1‑150 (MQ=60)
                                                              GCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAAC                                                                                        >  SRR19973072.2163635/1‑150 (MQ=60)
                                                                AGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCA                                                                                      >  SRR19973072.4547141/1‑150 (MQ=60)
                                                                AGTACAGACCTGAATTCAGGCTGGAGAGGGAAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCA                                                                                      >  SRR19973072.4467767/1‑150 (MQ=60)
                                                                    CAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAA                                                                                  >  SRR19973072.4315168/1‑150 (MQ=60)
                                                                     AGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAAC                                                                                 <  SRR19973072.3941380/150‑1 (MQ=60)
                                                                       ACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACAC                                                                               <  SRR19973072.19799/150‑1 (MQ=60)
                                                                       ACCTGAATTCAGGCTGGAGAGGGCAGAGGTCAGCACCACAATGTTCTTAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACAC                                                                               <  SRR19973072.2334674/150‑1 (MQ=60)
                                                                         CTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCA                                                                             >  SRR19973072.2529485/1‑150 (MQ=60)
                                                                             ATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGA                                                                         >  SRR19973072.3661770/1‑150 (MQ=60)
                                                                              TTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAG                                                                        <  SRR19973072.3927125/150‑1 (MQ=60)
                                                                              TTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAG                                                                        <  SRR19973072.618676/150‑1 (MQ=60)
                                                                              TTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAG                                                                        <  SRR19973072.9242/150‑1 (MQ=60)
                                                                               TCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGC                                                                       >  SRR19973072.2543415/1‑150 (MQ=60)
                                                                                CAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCC                                                                      >  SRR19973072.263923/1‑150 (MQ=60)
                                                                                CAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCC                                                                      <  SRR19973072.2625928/150‑1 (MQ=60)
                                                                                  GGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGA                                                                    <  SRR19973072.2789109/150‑1 (MQ=60)
                                                                                  GGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGA                                                                    <  SRR19973072.2789153/150‑1 (MQ=60)
                                                                                    CTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACG                                                                  <  SRR19973072.2324752/150‑1 (MQ=60)
                                                                                     TGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGT                                                                 >  SRR19973072.387791/1‑150 (MQ=60)
                                                                                      GGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTA                                                                <  SRR19973072.4064806/150‑1 (MQ=60)
                                                                                        AGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAA                                                              <  SRR19973072.3009507/150‑1 (MQ=60)
                                                                                         GAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAA                                                             <  SRR19973072.246490/150‑1 (MQ=60)
                                                                                         GAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAA                                                             <  SRR19973072.1877618/150‑1 (MQ=60)
                                                                                          AGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGTGAAAATCGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAAC                                                            >  SRR19973072.1916406/1‑150 (MQ=60)
                                                                                          AGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAAC                                                            <  SRR19973072.4367963/150‑1 (MQ=60)
                                                                                          AGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAAC                                                            <  SRR19973072.3226995/150‑1 (MQ=60)
                                                                                          AGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAAC                                                            <  SRR19973072.2389969/150‑1 (MQ=60)
                                                                                          AGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAAC                                                            <  SRR19973072.1425250/150‑1 (MQ=60)
                                                                                           GGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACA                                                           >  SRR19973072.3119841/1‑150 (MQ=60)
                                                                                            GGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAG                                                          <  SRR19973072.4633269/150‑1 (MQ=60)
                                                                                            GGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAG                                                          <  SRR19973072.4633255/150‑1 (MQ=60)
                                                                                              CAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGC                                                        >  SRR19973072.2265541/1‑150 (MQ=60)
                                                                                               AGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCC                                                       >  SRR19973072.582859/1‑150 (MQ=60)
                                                                                               AGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCC                                                       >  SRR19973072.1738573/1‑150 (MQ=60)
                                                                                               AGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCC                                                       >  SRR19973072.1738433/1‑150 (MQ=60)
                                                                                                GCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCA                                                      <  SRR19973072.2112435/150‑1 (MQ=60)
                                                                                                GCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCA                                                      <  SRR19973072.4188306/150‑1 (MQ=60)
                                                                                                  GGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAAT                                                    <  SRR19973072.703571/150‑1 (MQ=60)
                                                                                                  GGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAAT                                                    >  SRR19973072.3522571/1‑150 (MQ=60)
                                                                                                  GGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAAT                                                    >  SRR19973072.3522520/1‑150 (MQ=60)
                                                                                                  GGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAAT                                                    <  SRR19973072.2808698/150‑1 (MQ=60)
                                                                                                  GGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAAT                                                    >  SRR19973072.4598682/1‑150 (MQ=60)
                                                                                                   GTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACACAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATT                                                   <  SRR19973072.4197170/150‑1 (MQ=60)
                                                                                                    TCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATAC                                                  <  SRR19973072.2068813/150‑1 (MQ=60)
                                                                                                    TCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGAATTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATAC                                                  <  SRR19973072.311559/150‑1 (MQ=60)
                                                                                                     CAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACG                                                 >  SRR19973072.4610485/1‑150 (MQ=60)
                                                                                                      AGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGC                                                >  SRR19973072.3261690/1‑150 (MQ=60)
                                                                                                      AGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGC                                                >  SRR19973072.3261688/1‑150 (MQ=60)
                                                                                                       GCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCC                                               >  SRR19973072.538481/1‑150 (MQ=60)
                                                                                                       GCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCC                                               >  SRR19973072.538465/1‑150 (MQ=60)
                                                                                                            ACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATC                                          <  SRR19973072.799695/150‑1 (MQ=60)
                                                                                                               ATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACA                                       >  SRR19973072.3279810/1‑150 (MQ=60)
                                                                                                                TGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACAC                                      <  SRR19973072.4282455/150‑1 (MQ=60)
                                                                                                                 GTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACT                                     >  SRR19973072.718180/1‑150 (MQ=60)
                                                                                                                  TTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTG                                    <  SRR19973072.2103503/150‑1 (MQ=60)
                                                                                                                    CATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTT                                  >  SRR19973072.353892/1‑150 (MQ=60)
                                                                                                                      TAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAA                                <  SRR19973072.4418768/150‑1 (MQ=60)
                                                                                                                      TAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAA                                <  SRR19973072.4418744/150‑1 (MQ=60)
                                                                                                                       AATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAAT                               <  SRR19973072.3340717/150‑1 (MQ=60)
                                                                                                                        ATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATG                              >  SRR19973072.1126510/1‑150 (MQ=60)
                                                                                                                        ATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATG                              >  SRR19973072.1126247/1‑150 (MQ=60)
                                                                                                                           CTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCT                           >  SRR19973072.3581668/1‑150 (MQ=60)
                                                                                                                           CTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCT                           >  SRR19973072.2969723/1‑150 (MQ=60)
                                                                                                                           CTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCT                           >  SRR19973072.2969717/1‑150 (MQ=60)
                                                                                                                             GCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTT                         <  SRR19973072.1270266/150‑1 (MQ=60)
                                                                                                                             GCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTT                         <  SRR19973072.2714843/150‑1 (MQ=60)
                                                                                                                               CGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAG                       <  SRR19973072.294724/150‑1 (MQ=60)
                                                                                                                               CGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAG                       <  SRR19973072.2626554/150‑1 (MQ=60)
                                                                                                                               CGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAG                       <  SRR19973072.2626611/150‑1 (MQ=60)
                                                                                                                                GATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGG                      >  SRR19973072.984998/1‑150 (MQ=60)
                                                                                                                                GATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGG                      <  SRR19973072.253866/150‑1 (MQ=60)
                                                                                                                                GATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGG                      <  SRR19973072.2809430/150‑1 (MQ=60)
                                                                                                                                GATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGCCCAAAACGCCAAATCACACAGATAATGTCTTTAGG                      >  SRR19973072.2195235/1‑150 (MQ=60)
                                                                                                                                 ATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGC                     <  SRR19973072.4229965/150‑1 (MQ=60)
                                                                                                                                 ATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGC                     <  SRR19973072.3982429/150‑1 (MQ=60)
                                                                                                                                     ATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCA                 <  SRR19973072.2892579/150‑1 (MQ=60)
                                                                                                                                      TGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCACATCACACTGTTAATGGCTTTAGGCACCAT                >  SRR19973072.3546581/1‑150 (MQ=60)
                                                                                                                                         CACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGT             <  SRR19973072.245192/150‑1 (MQ=60)
                                                                                                                                           CACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCT           <  SRR19973072.3776640/150‑1 (MQ=60)
                                                                                                                                           CACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCT           <  SRR19973072.2808829/150‑1 (MQ=60)
                                                                                                                                           CACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCT           <  SRR19973072.3776729/150‑1 (MQ=60)
                                                                                                                                            ACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTG          <  SRR19973072.1654494/150‑1 (MQ=60)
                                                                                                                                            ACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTG          <  SRR19973072.1654472/150‑1 (MQ=60)
                                                                                                                                            ACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTG          <  SRR19973072.1654449/150‑1 (MQ=60)
                                                                                                                                               CAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGG       >  SRR19973072.22675/1‑150 (MQ=60)
                                                                                                                                                AGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGA      >  SRR19973072.2851516/1‑150 (MQ=60)
                                                                                                                                                  TTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATC    <  SRR19973072.1087793/150‑1 (MQ=60)
                                                                                                                                                  TTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATC    <  SRR19973072.1087763/150‑1 (MQ=60)
                                                                                                                                                   TAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCT   >  SRR19973072.4568764/1‑150 (MQ=60)
                                                                                                                                                   TAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCCGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCT   >  SRR19973072.4568802/1‑150 (MQ=60)
                                                                                                                                                    TAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTT  <  SRR19973072.2005554/150‑1 (MQ=60)
                                                                                                                                                    AAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTT  <  SRR19973072.4188829/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
ATTTTCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTT  >  CP009273/1519201‑1519498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑