Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,519,349 T→A 100% K297* (AAA→TAA)  ansP ← L‑asparagine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,519,3490TA99.3% 864.6 / ‑3.2 263K297* (AAA→TAA) ansPL‑asparagine transporter
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base A (127/134);  total (127/136)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 263 total aligned reads displayed.

TTTTCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGAT  >  CP009273/1519202‑1519495
                                                                                                                                                   |                                                                                                                                                  
  ttCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAagag                                                                                                                                                <  2:3586222/150‑1 (MQ=255)
    cGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGaa                                                                                                                                              >  2:3915081/1‑150 (MQ=255)
      ccATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAACGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGaaaa                                                                                                                                            <  2:4185494/150‑1 (MQ=255)
      ccACAACACACGGCGCGGAACCGCCCATCGCCAGTGACCGCCGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCGGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGaaaa                                                                                                                                            <  2:2811551/150‑1 (MQ=255)
       cATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGaaaaa                                                                                                                                           >  1:4165938/1‑150 (MQ=255)
       cATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGaaaaa                                                                                                                                           <  1:7447/150‑1 (MQ=255)
       cATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGaaaaa                                                                                                                                           >  2:1229175/1‑150 (MQ=255)
         tAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACg                                                                                                                                         <  2:3136219/150‑1 (MQ=255)
           aaaCTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTc                                                                                                                                       <  2:5252044/150‑1 (MQ=255)
           aaaCTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTc                                                                                                                                       >  2:5087307/1‑150 (MQ=255)
           aaaCTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTc                                                                                                                                       >  2:688160/1‑150 (MQ=255)
           aaaCTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTc                                                                                                                                       >  2:3260547/1‑150 (MQ=255)
            aaCTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCa                                                                                                                                      <  1:191468/150‑1 (MQ=255)
              cTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACg                                                                                                                                    <  2:2339988/150‑1 (MQ=255)
                  gTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACg                                                                                                                                <  2:3361700/150‑1 (MQ=255)
                  gTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACg                                                                                                                                <  1:5295700/150‑1 (MQ=255)
                  gTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACg                                                                                                                                <  1:4359368/150‑1 (MQ=255)
                  gTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACg                                                                                                                                >  2:4035070/1‑150 (MQ=255)
                   tGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACgg                                                                                                                               >  1:4855896/1‑150 (MQ=255)
                     cGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGAc                                                                                                                             <  1:5241695/150‑1 (MQ=255)
                      ggAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACt                                                                                                                            >  2:1599095/1‑150 (MQ=255)
                        aaCCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACttt                                                                                                                          <  2:421444/150‑1 (MQ=255)
                        aaCCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACttt                                                                                                                          <  1:4733312/150‑1 (MQ=255)
                            gCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGccc                                                                                                                      <  1:1435183/150‑1 (MQ=255)
                              ccATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCg                                                                                                                    <  1:1006348/150‑1 (MQ=255)
                               cATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGc                                                                                                                   <  1:4989666/150‑1 (MQ=255)
                                    ccATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGAt                                                                                                              <  1:1159455/150‑1 (MQ=255)
                                     cATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATa                                                                                                             >  2:491364/1‑150 (MQ=255)
                                     cATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATa                                                                                                             >  2:1453180/1‑150 (MQ=255)
                                      aTTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATAc                                                                                                            >  1:2015771/1‑150 (MQ=255)
                                         gAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATAcgcg                                                                                                         >  1:3922466/1‑150 (MQ=255)
                                               aGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCAc                                                                                                   >  1:1046933/1‑150 (MQ=255)
                                               aGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCAc                                                                                                   >  2:4000467/1‑150 (MQ=255)
                                                    aCGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCaa                                                                                              >  1:3297986/1‑150 (MQ=255)
                                                         cGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAataaca                                                                                         <  1:1648475/150‑1 (MQ=255)
                                                             gaaGTACAGACCTGAATTCAGGCTGTAGAGGGCAGCGGACAGCACCAAAATGTTCATAATGCTGCAGATATATGGCACACCCAGTTAAGACAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAataacataac                                                                                     <  1:1842084/148‑1 (MQ=255)
                                                             gCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCActaacataac                                                                                     >  1:4707764/1‑150 (MQ=255)
                                                              cAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAAcc                                                                                    <  2:1930611/150‑1 (MQ=255)
                                                                      aCCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAacac                                                                            <  1:1104908/150‑1 (MQ=255)
                                                                       ccTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAAcacc                                                                           >  1:4694052/1‑150 (MQ=255)
                                                                         tGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAAcaccac                                                                         <  2:4707108/150‑1 (MQ=255)
                                                                           aaTTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACgg                                                                       <  1:258729/150‑1 (MQ=255)
                                                                           aaTTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACgg                                                                       <  2:5081908/150‑1 (MQ=255)
                                                                             ttCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAg                                                                     <  2:4387248/150‑1 (MQ=255)
                                                                               cAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGcc                                                                   <  2:5213849/150‑1 (MQ=255)
                                                                               cAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAAAGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGcc                                                                   <  2:3310680/150‑1 (MQ=255)
                                                                                  gCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGAc                                                                <  1:1879058/150‑1 (MQ=255)
                                                                                       agagGGCAGCGGTCAGCACCACTATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTaaa                                                           <  1:1529695/150‑1 (MQ=255)
                                                                                         agGGCAGCGGTCAGCACCACAATGTACATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACTGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAAc                                                         <  2:2114498/150‑1 (MQ=255)
                                                                                          gggCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACa                                                        <  2:2777356/150‑1 (MQ=255)
                                                                                            gCAGCGGTCAGCCCCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAgg                                                      <  2:1706441/150‑1 (MQ=255)
                                                                                                 ggTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAAt                                                 >  2:309062/1‑150 (MQ=255)
                                                                                                  gTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATa                                                <  1:3260547/150‑1 (MQ=255)
                                                                                                  gTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATa                                                <  1:3453984/150‑1 (MQ=255)
                                                                                                   tCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATAc                                               <  2:1321693/150‑1 (MQ=255)
                                                                                                   tCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATAc                                               <  2:2638135/150‑1 (MQ=255)
                                                                                                     aGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGc                                             >  2:3647762/1‑150 (MQ=255)
                                                                                                      gCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGcc                                            <  1:2659604/150‑1 (MQ=255)
                                                                                                      gCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGcc                                            >  2:2589407/1‑150 (MQ=255)
                                                                                                      gCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGcc                                            <  1:726992/150‑1 (MQ=255)
                                                                                                       caccacAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCa                                           >  2:2939868/1‑150 (MQ=255)
                                                                                                       caccacAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTATGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCa                                           >  1:2220938/1‑150 (MQ=255)
                                                                                                        accacAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCaa                                          >  2:1297863/1‑150 (MQ=255)
                                                                                                        accacAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCaa                                          >  1:983427/1‑150 (MQ=255)
                                                                                                          cacaATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAAt                                        >  1:3020960/1‑150 (MQ=255)
                                                                                                          cacaATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAAt                                        >  2:2707111/1‑150 (MQ=255)
                                                                                                            caATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCACTAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATca                                      >  2:3419963/1‑150 (MQ=255)
                                                                                                            caATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATca                                      >  2:4261155/1‑150 (MQ=255)
                                                                                                            caATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATca                                      <  2:589098/150‑1 (MQ=255)
                                                                                                               tGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCacac                                   >  2:2493616/1‑150 (MQ=255)
                                                                                                                gTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACt                                  <  2:2444135/150‑1 (MQ=255)
                                                                                                                gTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACt                                  <  1:2437176/150‑1 (MQ=255)
                                                                                                                gTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACt                                  <  2:4662505/150‑1 (MQ=255)
                                                                                                                   cATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCCAATCACACTGtt                               >  2:4790408/1‑150 (MQ=255)
                                                                                                                   cATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGtt                               <  1:5118309/150‑1 (MQ=255)
                                                                                                                      aaTGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAAt                            >  2:3837442/1‑150 (MQ=255)
                                                                                                                       aTGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATg                           <  1:1694079/150‑1 (MQ=255)
                                                                                                                       aTGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATg                           <  2:2374338/150‑1 (MQ=255)
                                                                                                                           tgcCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCtt                       <  1:1460764/150‑1 (MQ=255)
                                                                                                                           tgcCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCtt                       <  1:2471245/150‑1 (MQ=255)
                                                                                                                             ccGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTa                     <  1:3053369/150‑1 (MQ=255)
                                                                                                                              cGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACTCCAAATCACACTGTTAATGGCTTTAg                    >  2:1806886/1‑150 (MQ=255)
                                                                                                                              cGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAg                    >  2:4846619/1‑150 (MQ=255)
                                                                                                                               gATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAgg                   >  1:4622430/1‑150 (MQ=255)
                                                                                                                                atatatGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGc                  >  2:1877831/1‑150 (MQ=255)
                                                                                                                                 tatatGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCa                 >  2:2682593/1‑150 (MQ=255)
                                                                                                                                  atatGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCAc                <  2:3718604/150‑1 (MQ=255)
                                                                                                                                    atGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCa              >  1:926654/1‑150 (MQ=255)
                                                                                                                                     tGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGTAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCAt             <  1:2175285/150‑1 (MQ=255)
                                                                                                                                      ggCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATg            <  2:3010206/150‑1 (MQ=255)
                                                                                                                                      ggCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATg            <  2:3010058/150‑1 (MQ=255)
                                                                                                                                       gCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATgg           <  1:2833137/150‑1 (MQ=255)
                                                                                                                                           acCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTg       >  1:3504076/1‑150 (MQ=255)
                                                                                                                                           acCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAAACAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTg       >  2:928601/1‑150 (MQ=255)
                                                                                                                                            cccAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGc      >  2:5278637/1‑150 (MQ=255)
                                                                                                                                             ccAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCg     <  2:1868778/150‑1 (MQ=255)
                                                                                                                                             ccAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCg     <  2:821914/150‑1 (MQ=255)
                                                                                                                                               aGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGa   >  1:374569/1‑150 (MQ=255)
                                                                                                                                                gTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGAt  <  2:2263031/150‑1 (MQ=255)
                                                                                                                                                gTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGAt  >  1:1479325/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTTTCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGAT  >  CP009273/1519202‑1519495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CATTTTCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTT  >  CP009273/1519200‑1519498
                                                                                                                                                     |                                                                                                                                                     
CATTTTCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTA                                                                                                                                                       >  SRR19973062.5241348/1‑150 (MQ=60)
CATTTTCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTA                                                                                                                                                       <  SRR19973062.4637874/150‑1 (MQ=60)
   TTTCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGA                                                                                                                                                    <  SRR19973062.2689219/150‑1 (MQ=60)
     TCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGA                                                                                                                                                  <  SRR19973062.4426819/150‑1 (MQ=60)
      CGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAA                                                                                                                                                 <  SRR19973062.2267844/150‑1 (MQ=60)
      CGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAA                                                                                                                                                 >  SRR19973062.5276545/1‑150 (MQ=60)
       GCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAA                                                                                                                                                <  SRR19973062.262246/150‑1 (MQ=60)
        CCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAA                                                                                                                                               >  SRR19973062.2014412/1‑150 (MQ=60)
        CCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAA                                                                                                                                               >  SRR19973062.1443265/1‑150 (MQ=60)
        CCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAA                                                                                                                                               >  SRR19973062.1443279/1‑150 (MQ=60)
         CATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAA                                                                                                                                              >  SRR19973062.4187556/1‑150 (MQ=60)
         CATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAA                                                                                                                                              <  SRR19973062.7504/150‑1 (MQ=60)
          ATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAAC                                                                                                                                             <  SRR19973062.3217619/150‑1 (MQ=60)
            AAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGT                                                                                                                                           >  SRR19973062.2043847/1‑150 (MQ=60)
            AAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGT                                                                                                                                           <  SRR19973062.2421384/150‑1 (MQ=60)
             AAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTC                                                                                                                                          <  SRR19973062.4736918/150‑1 (MQ=60)
              AACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCA                                                                                                                                         <  SRR19973062.192511/150‑1 (MQ=60)
               ACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCAC                                                                                                                                        <  SRR19973062.5353443/150‑1 (MQ=60)
                   GGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAAC                                                                                                                                    >  SRR19973062.3535853/1‑150 (MQ=60)
                   GGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAAC                                                                                                                                    >  SRR19973062.4659891/1‑150 (MQ=60)
                   GGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAAC                                                                                                                                    >  SRR19973062.1913570/1‑150 (MQ=60)
                   GGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAAC                                                                                                                                    >  SRR19973062.1913525/1‑150 (MQ=60)
                    GTGCGGAACCGCCCATCGCCATTGAGCGCAGTATACGTCCGGTGAAGTACAGACCTGAATTCTGGCTGGAGAGGGCAGCGGTCAGCACCAAAATGTTCATAATGCTGCCGTTATATGGCAAACCCTGTTAAGAGAAAAACGTCACGAACG                                                                                                                                   <  SRR19973062.5061935/150‑1 (MQ=60)
                    GTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACG                                                                                                                                   <  SRR19973062.5323523/150‑1 (MQ=60)
                    GTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACG                                                                                                                                   <  SRR19973062.4381756/150‑1 (MQ=60)
                    GTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACG                                                                                                                                   <  SRR19973062.4381759/150‑1 (MQ=60)
                    GTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACG                                                                                                                                   <  SRR19973062.5276004/150‑1 (MQ=60)
                     TGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGG                                                                                                                                  >  SRR19973062.3777477/1‑150 (MQ=60)
                     TGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGG                                                                                                                                  >  SRR19973062.4880953/1‑150 (MQ=60)
                       CGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGAC                                                                                                                                <  SRR19973062.5269304/150‑1 (MQ=60)
                        GGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACT                                                                                                                               >  SRR19973062.738930/1‑150 (MQ=60)
                          AACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTT                                                                                                                             <  SRR19973062.4757994/150‑1 (MQ=60)
                          AACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTT                                                                                                                             <  SRR19973062.4336531/150‑1 (MQ=60)
                          AACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTT                                                                                                                             <  SRR19973062.1488825/150‑1 (MQ=60)
                           ACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTG                                                                                                                            <  SRR19973062.283610/150‑1 (MQ=60)
                              GCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCC                                                                                                                         <  SRR19973062.1442147/150‑1 (MQ=60)
                                CCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCG                                                                                                                       <  SRR19973062.1011049/150‑1 (MQ=60)
                                 CATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGC                                                                                                                      >  SRR19973062.501827/1‑150 (MQ=60)
                                 CATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGC                                                                                                                      <  SRR19973062.5016029/150‑1 (MQ=60)
                                    CGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTG                                                                                                                   <  SRR19973062.4906335/150‑1 (MQ=60)
                                      CCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGAT                                                                                                                 <  SRR19973062.414283/150‑1 (MQ=60)
                                      CCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGAT                                                                                                                 <  SRR19973062.1164888/150‑1 (MQ=60)
                                        ATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATAC                                                                                                               >  SRR19973062.2026131/1‑150 (MQ=60)
                                        ATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATAC                                                                                                               <  SRR19973062.2583099/150‑1 (MQ=60)
                                           GAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCG                                                                                                            >  SRR19973062.3942420/1‑150 (MQ=60)
                                               GCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCC                                                                                                        <  SRR19973062.12768/150‑1 (MQ=60)
                                                CAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCA                                                                                                       >  SRR19973062.4731048/1‑150 (MQ=60)
                                                 AGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCAC                                                                                                      >  SRR19973062.1051852/1‑150 (MQ=60)
                                                 AGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCAC                                                                                                      >  SRR19973062.813133/1‑150 (MQ=60)
                                                    ATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGC                                                                                                   >  SRR19973062.5180752/1‑150 (MQ=60)
                                                      ACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAA                                                                                                 >  SRR19973062.3315055/1‑150 (MQ=60)
                                                        GTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATA                                                                                               <  SRR19973062.1107626/150‑1 (MQ=60)
                                                          CCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAAC                                                                                             >  SRR19973062.2451458/1‑150 (MQ=60)
                                                           CGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACA                                                                                            <  SRR19973062.1656598/150‑1 (MQ=60)
                                                               GCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCACTAACATAAC                                                                                        >  SRR19973062.4732343/1‑150 (MQ=60)
                                                               GAAGTACAGACCTGAATTCAGGCTGTAGAGGGCAGCGGACAGCACCAAAATGTTCATAATGCTGCAGATATATGGCACACCCAGTTAAGACAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAAC                                                                                        <  SRR19973062.1851268/150‑1 (MQ=60)
                                                                CAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACC                                                                                       >  SRR19973062.907928/1‑150 (MQ=60)
                                                                     CAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAA                                                                                  >  SRR19973062.2596092/1‑150 (MQ=60)
                                                                      AGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAAC                                                                                 >  SRR19973062.2888290/1‑150 (MQ=60)
                                                                        ACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACAC                                                                               <  SRR19973062.1110104/150‑1 (MQ=60)
                                                                         CCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACC                                                                              >  SRR19973062.4718563/1‑150 (MQ=60)
                                                                         CCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACC                                                                              >  SRR19973062.4718576/1‑150 (MQ=60)
                                                                         CCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACC                                                                              <  SRR19973062.1547433/150‑1 (MQ=60)
                                                                            GAATTCTGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACG                                                                           <  SRR19973062.4838225/150‑1 (MQ=60)
                                                                            GAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACG                                                                           <  SRR19973062.477580/150‑1 (MQ=60)
                                                                             AATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGG                                                                          >  SRR19973062.1266296/1‑150 (MQ=60)
                                                                             AATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGG                                                                          <  SRR19973062.260028/150‑1 (MQ=60)
                                                                                    GCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGAC                                                                   <  SRR19973062.1888110/150‑1 (MQ=60)
                                                                                    GCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGAC                                                                   <  SRR19973062.1888576/150‑1 (MQ=60)
                                                                                         AGAGGGCAGCGGTCAGCACCACTATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAA                                                              <  SRR19973062.1537237/150‑1 (MQ=60)
                                                                                           AGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAAC                                                            >  SRR19973062.1049918/1‑150 (MQ=60)
                                                                                                AGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCC                                                       >  SRR19973062.114026/1‑150 (MQ=60)
                                                                                                    GTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATA                                                   <  SRR19973062.3471601/150‑1 (MQ=60)
                                                                                                    GTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATA                                                   <  SRR19973062.3471590/150‑1 (MQ=60)
                                                                                                    GTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATA                                                   <  SRR19973062.3277553/150‑1 (MQ=60)
                                                                                                    GTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATA                                                   <  SRR19973062.3277462/150‑1 (MQ=60)
                                                                                                     TCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATAC                                                  <  SRR19973062.348734/150‑1 (MQ=60)
                                                                                                     TCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATAC                                                  >  SRR19973062.1494752/1‑150 (MQ=60)
                                                                                                      CAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACG                                                 >  SRR19973062.3046925/1‑150 (MQ=60)
                                                                                                       AGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGC                                                >  SRR19973062.4747332/1‑150 (MQ=60)
                                                                                                        GCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCC                                               <  SRR19973062.2673564/150‑1 (MQ=60)
                                                                                                        GCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCC                                               >  SRR19973062.1799957/1‑150 (MQ=60)
                                                                                                        GCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCC                                               <  SRR19973062.730309/150‑1 (MQ=60)
                                                                                                         CACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTATGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCA                                              >  SRR19973062.2232062/1‑150 (MQ=60)
                                                                                                          ACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAA                                             >  SRR19973062.987988/1‑150 (MQ=60)
                                                                                                           CCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAA                                            <  SRR19973062.3302064/150‑1 (MQ=60)
                                                                                                           CCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAA                                            <  SRR19973062.394182/150‑1 (MQ=60)
                                                                                                            CACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAAT                                           <  SRR19973062.2975417/150‑1 (MQ=60)
                                                                                                            CACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAAT                                           >  SRR19973062.3036731/1‑150 (MQ=60)
                                                                                                             AAAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATC                                          <  SRR19973062.525723/150‑1 (MQ=60)
                                                                                                                ATGTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACA                                       >  SRR19973062.108026/1‑150 (MQ=60)
                                                                                                                  GTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACT                                     <  SRR19973062.2450257/150‑1 (MQ=60)
                                                                                                                  GTTCATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACT                                     <  SRR19973062.1607026/150‑1 (MQ=60)
                                                                                                                     CATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTT                                  >  SRR19973062.627748/1‑150 (MQ=60)
                                                                                                                     CATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTT                                  <  SRR19973062.5145393/150‑1 (MQ=60)
                                                                                                                      ATAATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTA                                 >  SRR19973062.1695116/1‑150 (MQ=60)
                                                                                                                         ATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATG                              <  SRR19973062.1702382/150‑1 (MQ=60)
                                                                                                                         ATGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATG                              >  SRR19973062.176209/1‑150 (MQ=60)
                                                                                                                          TGCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGG                             <  SRR19973062.3092379/150‑1 (MQ=60)
                                                                                                                           GCTGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGC                            >  SRR19973062.1958009/1‑150 (MQ=60)
                                                                                                                             TGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTT                          <  SRR19973062.357212/150‑1 (MQ=60)
                                                                                                                             TGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTT                          <  SRR19973062.1478635/150‑1 (MQ=60)
                                                                                                                             TGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTT                          <  SRR19973062.1467901/150‑1 (MQ=60)
                                                                                                                             TGCCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTT                          <  SRR19973062.2484486/150‑1 (MQ=60)
                                                                                                                               CCGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTA                        <  SRR19973062.3069228/150‑1 (MQ=60)
                                                                                                                                CGATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAG                       <  SRR19973062.947999/150‑1 (MQ=60)
                                                                                                                                 GATATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGG                      >  SRR19973062.4646612/1‑150 (MQ=60)
                                                                                                                                    ATATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCAC                   <  SRR19973062.975948/150‑1 (MQ=60)
                                                                                                                                      ATGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCA                 >  SRR19973062.930779/1‑150 (MQ=60)
                                                                                                                                       TGGCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGTAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCAT                <  SRR19973062.2186257/150‑1 (MQ=60)
                                                                                                                                         GCACACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGG              <  SRR19973062.2848124/150‑1 (MQ=60)
                                                                                                                                             ACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTG          >  SRR19973062.3521854/1‑150 (MQ=60)
                                                                                                                                             ACCCAGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTG          >  SRR19973062.3521845/1‑150 (MQ=60)
                                                                                                                                                 AGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGA      >  SRR19973062.376242/1‑150 (MQ=60)
                                                                                                                                                 AGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGA      <  SRR19973062.2204283/150‑1 (MQ=60)
                                                                                                                                                 AGTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGA      <  SRR19973062.2204253/150‑1 (MQ=60)
                                                                                                                                                  GTTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGAT     >  SRR19973062.1486578/1‑150 (MQ=60)
                                                                                                                                                   TTAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATC    <  SRR19973062.493638/150‑1 (MQ=60)
                                                                                                                                                    TAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCT   >  SRR19973062.48184/1‑150 (MQ=60)
                                                                                                                                                    TAAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAAAAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCA   >  SRR19973062.612823/1‑150 (MQ=60)
                                                                                                                                                     CAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTT  <  SRR19973062.127903/150‑1 (MQ=60)
                                                                                                                                                     AAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATCCGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTT  <  SRR19973062.2559280/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CATTTTCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTT  >  CP009273/1519200‑1519498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑