Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,554,475 T→A 30.7% T238S (ACC→TCC)  ddpB ← D‑ala‑D‑ala transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,554,4750TA30.7% 402.4 / 251.1 257T238S (ACC→TCC) ddpBD‑ala‑D‑ala transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (92/86);  new base A (41/38);  total (133/124)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 259 total aligned reads displayed.

GGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCG  >  CP009273/1554326‑1554624
                                                                                                                                                     |                                                                                                                                                     
 ggCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTa                                                                                                                                                      <  1:2272021/150‑1 (MQ=255)
  gCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATCCGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGAAc                                                                                                                                                     <  2:1967685/150‑1 (MQ=255)
  gCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTAc                                                                                                                                                     <  1:4550068/150‑1 (MQ=255)
  gCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGAAc                                                                                                                                                     <  2:1578842/150‑1 (MQ=255)
     aGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGaa                                                                                                                                                  >  1:3288657/1‑150 (MQ=255)
       gCAAACACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACCTAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATg                                                                                                                                                <  2:4432064/150‑1 (MQ=255)
       gCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATg                                                                                                                                                <  1:156943/150‑1 (MQ=255)
        cAAACACTGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATCCGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAATACGTTATTCCAGCCGGGCAAGCCGCTGGCCCGGGCGGAACGAATGt                                                                                                                                               <  1:3415480/150‑1 (MQ=255)
          aaaaCGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAg                                                                                                                                             <  1:4388774/150‑1 (MQ=255)
           aaaCGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGt                                                                                                                                            <  2:3613850/150‑1 (MQ=255)
                         aGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGt                                                                                                                              >  2:3185728/1‑150 (MQ=255)
                          gCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTCGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGAACGAATGTAGTCTTCACTCAATTGtt                                                                                                                             >  2:1278387/1‑150 (MQ=255)
                            aCTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGAACGAATGTAGTCTTCACTCAATTGTTcc                                                                                                                           >  1:1974996/1‑150 (MQ=255)
                              tGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAg                                                                                                                         >  1:2569407/1‑150 (MQ=255)
                              tGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGAACGAATGTAGTCTTCACTCAATTGTTCCAg                                                                                                                         <  1:1413474/150‑1 (MQ=255)
                              tGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGAACGAATGTAGTCTTCACTCAATTGTTCCAg                                                                                                                         <  1:2845566/150‑1 (MQ=255)
                                 gcCATACCACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCCACGCATTGGGTAGCGCATACCATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCAt                                                                                                                      <  2:331162/150‑1 (MQ=255)
                                   cATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGAACGAATGTAGTCTTCACTCAATTGTTCCAGCATCg                                                                                                                    >  1:1001880/1‑150 (MQ=255)
                                    aTACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGc                                                                                                                   >  2:1767534/1‑150 (MQ=255)
                                    aTACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGc                                                                                                                   >  1:2611164/1‑150 (MQ=255)
                                     tACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCt                                                                                                                  >  2:3921892/1‑150 (MQ=255)
                                          caaATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGAACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCg                                                                                                             >  1:54795/1‑150 (MQ=255)
                                          caaATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGAACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCg                                                                                                             <  2:4331613/150‑1 (MQ=255)
                                             aTCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGAt                                                                                                          >  2:4457815/1‑150 (MQ=255)
                                             aTCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGAt                                                                                                          >  2:4156606/1‑150 (MQ=255)
                                              tCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGAACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATc                                                                                                         >  1:3763088/1‑150 (MQ=255)
                                               cGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGAACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCt                                                                                                        >  2:3521079/1‑150 (MQ=255)
                                                gCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTg                                                                                                       >  2:2686451/1‑150 (MQ=255)
                                                gCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTg                                                                                                       >  2:1903645/1‑150 (MQ=255)
                                                       gcCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGAACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGgcgagcg                                                                                                >  2:284157/1‑150 (MQ=255)
                                                                aaTACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTccc                                                                                       >  1:1851018/1‑150 (MQ=255)
                                                                    cGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGAACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGt                                                                                   <  2:1669322/150‑1 (MQ=255)
                                                                        aaTCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTgaac                                                                               <  2:4138566/150‑1 (MQ=255)
                                                                         aTCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGAACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTgaacg                                                                              <  2:899848/150‑1 (MQ=255)
                                                                           cGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCACTGGCCCGGGCGGAACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTgaacgaa                                                                            >  2:2386166/1‑150 (MQ=255)
                                                                            gATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTgaacgaac                                                                           >  2:2587513/1‑150 (MQ=255)
                                                                                  aTCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGAACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGc                                                                     <  1:964792/150‑1 (MQ=255)
                                                                                   tCAACGCATTGGGTCGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCg                                                                    >  1:3937473/1‑150 (MQ=255)
                                                                                    cAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGt                                                                   >  1:2796989/1‑150 (MQ=255)
                                                                                     aaCGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGtt                                                                  <  2:146383/150‑1 (MQ=255)
                                                                                     aaCGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGtt                                                                  <  2:4084815/150‑1 (MQ=255)
                                                                                     aaCGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGtt                                                                  >  1:4009077/1‑150 (MQ=255)
                                                                                     aaCGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGAACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGtt                                                                  <  1:1278387/150‑1 (MQ=255)
                                                                                     aaCGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGAACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGtt                                                                  <  1:2454819/150‑1 (MQ=255)
                                                                                      aCGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGAACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTa                                                                 >  1:3860226/1‑150 (MQ=255)
                                                                                        gCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAAt                                                               >  1:597214/1‑150 (MQ=255)
                                                                                        gCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAAt                                                               <  2:3938524/150‑1 (MQ=255)
                                                                                        gCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAAt                                                               <  2:3914873/150‑1 (MQ=255)
                                                                                         cATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATg                                                              <  2:3264907/150‑1 (MQ=255)
                                                                                          aTTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGc                                                             >  2:1487004/1‑150 (MQ=255)
                                                                                           ttGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGAACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGcc                                                            <  2:2818831/150‑1 (MQ=255)
                                                                                              ggTAGCGCATAACATAACACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGt                                                         <  2:2645645/150‑1 (MQ=255)
                                                                                               gTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTa                                                        <  2:1433445/150‑1 (MQ=255)
                                                                                               gTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTa                                                        <  2:3181916/150‑1 (MQ=255)
                                                                                               gTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTa                                                        >  1:3775613/1‑150 (MQ=255)
                                                                                                    gcATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAagatg                                                   <  1:4188502/150‑1 (MQ=255)
                                                                                                     cataacataaAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAagatga                                                  >  2:384232/1‑150 (MQ=255)
                                                                                                      ataacataaAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGAACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAagatgag                                                 >  2:2183952/1‑150 (MQ=255)
                                                                                                       taacataaAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAagatgaga                                                >  2:1703005/1‑150 (MQ=255)
                                                                                                             aaaaCGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGc                                          <  2:1197315/150‑1 (MQ=255)
                                                                                                              aaaCGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCa                                         >  2:3664923/1‑150 (MQ=255)
                                                                                                              aaaCGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGAACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCa                                         >  2:2324994/1‑150 (MQ=255)
                                                                                                                 cGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACg                                      >  2:2555422/1‑150 (MQ=255)
                                                                                                                  gATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGc                                     >  1:2415316/1‑150 (MQ=255)
                                                                                                                  gATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGAACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGc                                     <  1:4030707/150‑1 (MQ=255)
                                                                                                                     ataCCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCAtt                                  >  1:1634461/1‑150 (MQ=255)
                                                                                                                      taCCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTg                                 <  1:4428287/150‑1 (MQ=255)
                                                                                                                      taCCAGCCGGGCAAGCCGCTGGCCCGGGCGGAACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTg                                 <  1:210481/150‑1 (MQ=255)
                                                                                                                        ccAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTgaa                               >  2:1084700/1‑150 (MQ=255)
                                                                                                                         cAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTgaag                              >  1:116208/1‑150 (MQ=255)
                                                                                                                         cAGCCGGGCAAGCCGCTGGCCCGGGCGGAACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTgaag                              >  1:1902797/1‑150 (MQ=255)
                                                                                                                          aGCCGGGCAAGCCGCTGGCCCGGGCGGAACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTgaaga                             >  2:4607725/1‑150 (MQ=255)
                                                                                                                             ggggCAAGCCGCTGGCCCGGGCGGTACGTATGTAGTCTTCACTCAATTGTTCCAGCATCTCTGAGCGTATTTTGCGAGCGACAATTCACAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAtgaaga                          <  2:1897990/149‑1 (MQ=255)
                                                                                                                             cGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAagaaga                          <  2:3105819/150‑1 (MQ=255)
                                                                                                                              gtgCAAGCCTGTGGCCCTTGCGGAACGTATGTAGTTTTCTCTCAATTGTTCCTGCATCGATGATCTTATCTGGCGAGAGACTCTTCCCAGGTGTAAGAACTCCATCGTTAATGCCGGTAAGATTAGATGTTGCAACGCATTGAagatgaa                         <  2:614541/148‑2 (MQ=255)
                                                                                                                              gggCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTCAGATGAGATGTTGCAACGCATTGAAGAAGAc                         >  1:324259/1‑150 (MQ=255)
                                                                                                                               ggCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACt                        <  2:4001704/150‑1 (MQ=255)
                                                                                                                                  aaGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCa                     >  1:3603344/1‑150 (MQ=255)
                                                                                                                                  aaGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCa                     <  1:1767534/150‑1 (MQ=255)
                                                                                                                                  aaGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCa                     >  1:1511252/1‑150 (MQ=255)
                                                                                                                                  aaGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAAGTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGACCGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCa                     >  2:23882/1‑150 (MQ=255)
                                                                                                                                  aaGCCGCTGGCCCGGGCGGAACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCa                     >  2:4390803/1‑150 (MQ=255)
                                                                                                                                  aaGCCGCTGGCCCGGGCGGAACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCa                     >  2:2463021/1‑150 (MQ=255)
                                                                                                                                       gCTGGCCCGGGCGGTACGATTGTAGTATTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTTAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGtt                <  2:1331447/150‑1 (MQ=255)
                                                                                                                                       gCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGtt                <  2:692604/150‑1 (MQ=255)
                                                                                                                                         tGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGc              >  2:2859293/1‑150 (MQ=255)
                                                                                                                                         tGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGc              >  2:3929819/1‑150 (MQ=255)
                                                                                                                                           gCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCt            >  1:3106000/1‑150 (MQ=255)
                                                                                                                                            cccGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGTCAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATTTTGCAACTAATTGTAGAAGACTTCACCGTTGCCtt           <  2:3424077/150‑1 (MQ=255)
                                                                                                                                            cccGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCtt           <  2:4009077/150‑1 (MQ=255)
                                                                                                                                            cccGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCtt           <  1:1374899/150‑1 (MQ=255)
                                                                                                                                            cccGGGCGGAACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCtt           >  1:1801488/1‑150 (MQ=255)
                                                                                                                                                ggCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAag       <  2:1751586/150‑1 (MQ=255)
                                                                                                                                                 gCGGAACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAagc      <  1:903444/150‑1 (MQ=255)
                                                                                                                                                  cGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAagca     <  1:2220497/150‑1 (MQ=255)
                                                                                                                                                  cGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAagca     >  1:3049031/1‑150 (MQ=255)
                                                                                                                                                  cGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAagca     >  1:661552/1‑150 (MQ=255)
                                                                                                                                                  cGGTACGAATGTAGTCTTCACTCAATTGGTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAagca     <  1:429349/150‑1 (MQ=255)
                                                                                                                                                  cGGAACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAagca     <  2:1606663/150‑1 (MQ=255)
                                                                                                                                                     tACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAgcg  >  1:1342945/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGGCCAGGCAAAAACGGTTTCGGTGAGCACTGCGCCATACAACAAATCGCCCAGCGCCAAACCCAATACGGTAATCGATGGGATCAACGCATTGGGTAGCGCATAACATAAAACGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCG  >  CP009273/1554326‑1554624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A