Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,554,588 G→T 38.3% A200E (GCG→GAG)  ddpB ← D‑ala‑D‑ala transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,554,5880GT38.3% 331.9 / 250.7 237A200E (GCG→GAG) ddpBD‑ala‑D‑ala transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (72/74);  new base T (34/57);  total (106/131)
Fisher's exact test for biased strand distribution p-value = 8.15e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 237 total aligned reads displayed.

GATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGACCATAAAACAGCACAATGACGCCCAG  >  CP009273/1554440‑1554736
                                                                                                                                                    |                                                                                                                                                    
gATAAACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATAGCTGAGCGGATCTGGCGAGCGACAATACCCAGGTGAACGAACGCCAGCGATAATGCCGGTCAGATGAGATGTTGCAACGc                                                                                                                                                     >  1:3057279/1‑150 (MQ=255)
    taCCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTg                                                                                                                                                 >  1:1730766/1‑150 (MQ=255)
    taCCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTg                                                                                                                                                 >  2:2505758/1‑150 (MQ=255)
        aGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTgaaga                                                                                                                                             >  1:1793556/1‑150 (MQ=255)
          ccGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGaagaag                                                                                                                                           <  1:2553798/150‑1 (MQ=255)
           cGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAagaaga                                                                                                                                          >  2:3216750/1‑150 (MQ=255)
           cGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAagaaga                                                                                                                                          >  2:2294832/1‑150 (MQ=255)
             ggCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACt                                                                                                                                        <  2:4036774/150‑1 (MQ=255)
             ggCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACt                                                                                                                                        >  1:501279/1‑150 (MQ=255)
             ggCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAAGTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACt                                                                                                                                        <  2:889347/150‑1 (MQ=255)
                 aGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACTATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCAc                                                                                                                                    <  2:1988377/150‑1 (MQ=255)
                 aGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGCTGTTGCAACGCATTGAAGAAGACTTCAc                                                                                                                                    <  2:4087404/150‑1 (MQ=255)
                  gCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCAcc                                                                                                                                   <  1:1568162/150‑1 (MQ=255)
                   ccGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCg                                                                                                                                  >  2:3287034/1‑150 (MQ=255)
                     gCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGtt                                                                                                                                <  2:902550/150‑1 (MQ=255)
                     gCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGtt                                                                                                                                >  2:1633501/1‑150 (MQ=255)
                      cTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTg                                                                                                                               <  1:2193008/150‑1 (MQ=255)
                       tGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGc                                                                                                                              <  2:2763960/150‑1 (MQ=255)
                         gCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCt                                                                                                                            <  2:1807539/150‑1 (MQ=255)
                           ccGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTc                                                                                                                          <  2:1571094/150‑1 (MQ=255)
                                cGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAagca                                                                                                                     >  2:3199395/1‑150 (MQ=255)
                                cGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAagca                                                                                                                     >  2:1756176/1‑150 (MQ=255)
                                      gAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCAt                                                                                                               >  1:658365/1‑150 (MQ=255)
                                        aTGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCg                                                                                                             >  2:2459862/1‑150 (MQ=255)
                                         tGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGa                                                                                                            >  1:810886/1‑150 (MQ=255)
                                          gTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAg                                                                                                           >  1:2561625/1‑150 (MQ=255)
                                           tAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCTAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGc                                                                                                          >  1:1676655/1‑150 (MQ=255)
                                           tAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCATCAAGCAGCGCATCGAGc                                                                                                          >  2:4150064/1‑150 (MQ=255)
                                            aGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCa                                                                                                         <  1:112694/150‑1 (MQ=255)
                                              tCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGa                                                                                                       >  2:214910/1‑150 (MQ=255)
                                               cTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGAt                                                                                                      >  1:942947/1‑150 (MQ=255)
                                                ttCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATa                                                                                                     <  1:1512369/150‑1 (MQ=255)
                                                  cACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATaaa                                                                                                   >  1:2944947/1‑150 (MQ=255)
                                                      cAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGcc                                                                                               >  1:222855/1‑150 (MQ=255)
                                                        aTTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCgg                                                                                             >  1:984440/1‑150 (MQ=255)
                                                         ttGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGt                                                                                            <  1:2240001/150‑1 (MQ=255)
                                                           gTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTaa                                                                                          <  1:3110687/150‑1 (MQ=255)
                                                           gTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTaa                                                                                          <  1:558899/150‑1 (MQ=255)
                                                           gTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTaa                                                                                          >  1:1321393/1‑150 (MQ=255)
                                                                   aTCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGtt                                                                                  >  1:391043/1‑150 (MQ=255)
                                                                    tCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTtg                                                                                 <  2:2236052/150‑1 (MQ=255)
                                                                       cTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTtggtg                                                                              <  1:3236322/150‑1 (MQ=255)
                                                                         gAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGa                                                                            <  1:987751/150‑1 (MQ=255)
                                                                            cGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATcc                                                                         <  1:2841066/150‑1 (MQ=255)
                                                                            cGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGGAAGTTGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATcc                                                                         >  2:727227/1‑150 (MQ=255)
                                                                             ggATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATcca                                                                        >  2:392514/1‑150 (MQ=255)
                                                                             ggATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATcca                                                                        <  2:116068/150‑1 (MQ=255)
                                                                              gATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATccag                                                                       >  1:1692579/1‑150 (MQ=255)
                                                                              gATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATccag                                                                       <  2:3449169/150‑1 (MQ=255)
                                                                               aTCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATccagc                                                                      <  1:4126404/150‑1 (MQ=255)
                                                                               aTCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATccagc                                                                      >  1:114677/1‑150 (MQ=255)
                                                                                 cTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATccagcca                                                                    <  2:655941/150‑1 (MQ=255)
                                                                                  tGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATccagccag                                                                   >  1:1312078/1‑150 (MQ=255)
                                                                                  tGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATccagccag                                                                   >  2:1053260/1‑150 (MQ=255)
                                                                                  tGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATccagccag                                                                   <  1:2087245/150‑1 (MQ=255)
                                                                                     cgagcgaCAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGtca                                                                <  2:2594648/150‑1 (MQ=255)
                                                                                     cgagcgaCAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGtca                                                                >  2:102388/1‑150 (MQ=255)
                                                                                     cgagcgaCAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGtca                                                                <  1:4108859/150‑1 (MQ=255)
                                                                                      gagcgaCAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGtcat                                                               <  2:39893/150‑1 (MQ=255)
                                                                                       agcgaCAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGtcatc                                                              >  2:1825193/1‑150 (MQ=255)
                                                                                          gaCAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAg                                                           <  1:101821/150‑1 (MQ=255)
                                                                                           aCAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGc                                                          <  1:2382852/150‑1 (MQ=255)
                                                                                           aCAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGc                                                          <  1:2648381/150‑1 (MQ=255)
                                                                                            cAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGcc                                                         <  1:2577926/150‑1 (MQ=255)
                                                                                            cAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGcc                                                         <  2:1094191/150‑1 (MQ=255)
                                                                                             aaTTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCt                                                        <  1:2876389/150‑1 (MQ=255)
                                                                                             aaTTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCt                                                        >  2:478036/1‑150 (MQ=255)
                                                                                             aaTTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCt                                                        >  2:2435589/1‑150 (MQ=255)
                                                                                                tCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTcc                                                     <  2:3299365/150‑1 (MQ=255)
                                                                                                    aGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTccgccg                                                 <  2:634190/150‑1 (MQ=255)
                                                                                                    aGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTccgccg                                                 >  1:3451403/1‑150 (MQ=255)
                                                                                                      gTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTccgccgcc                                               <  1:3088490/150‑1 (MQ=255)
                                                                                                        gaacgaacGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCgg                                             >  1:3637154/1‑150 (MQ=255)
                                                                                                           cgaacgCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGgaa                                          <  1:2370353/150‑1 (MQ=255)
                                                                                                           cgaacgCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGgaa                                          <  2:514908/150‑1 (MQ=255)
                                                                                                           cgaacgCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGgaa                                          <  2:1880045/150‑1 (MQ=255)
                                                                                                              acgCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGgaagaa                                       <  2:1500927/150‑1 (MQ=255)
                                                                                                              acgCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGgaagaa                                       <  2:3597551/150‑1 (MQ=255)
                                                                                                               cgCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAAt                                      <  1:336582/150‑1 (MQ=255)
                                                                                                                gCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAAtt                                     <  2:179940/150‑1 (MQ=255)
                                                                                                                     cGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAg                                <  2:3046989/150‑1 (MQ=255)
                                                                                                                      gTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGa                               >  1:197936/1‑150 (MQ=255)
                                                                                                                         aaTGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGa                            <  1:877131/150‑1 (MQ=255)
                                                                                                                           tGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGAcc                          >  1:3968267/1‑150 (MQ=255)
                                                                                                                              cGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGACCATa                       <  2:3115743/150‑1 (MQ=255)
                                                                                                                                gTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGACCATaaa                     >  2:3155775/1‑150 (MQ=255)
                                                                                                                                   agatgagatgTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGACCATAAAACa                  >  2:2458915/1‑150 (MQ=255)
                                                                                                                                     atgagatgTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGACCATAAAACAGc                <  2:2112226/150‑1 (MQ=255)
                                                                                                                                      tgagatgTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGACCATAAAACAGca               <  2:3830575/150‑1 (MQ=255)
                                                                                                                                       gagatgTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGACCATAAAACAGcac              >  2:366620/1‑150 (MQ=255)
                                                                                                                                       gagatgTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGACCATAAAACAGcac              <  2:401567/150‑1 (MQ=255)
                                                                                                                                       gagatgTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGACCATAAAACAGcac              >  2:3178072/1‑150 (MQ=255)
                                                                                                                                           tgTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGACCATAAAACAGCACAATg          <  2:3922432/150‑1 (MQ=255)
                                                                                                                                           tgTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGACCATAAAACAGCACAATg          <  2:1318605/150‑1 (MQ=255)
                                                                                                                                              tGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGATCAGATATAAGCCGGTTACGTGTGTTGTTGGATCCAGCCAGTCATCAAGCCTTCCGCCGACGGTAAGATTTTGCAGATGACCATAAAACAGCACAATGACg       <  2:2961126/150‑1 (MQ=255)
                                                                                                                                                cAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGACCATAAAACAGCACAATGACgcc     <  1:1853755/150‑1 (MQ=255)
                                                                                                                                                cAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGACCATAAAACAGCACAATGACgcc     >  2:2913893/1‑150 (MQ=255)
                                                                                                                                                 aaCGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGACGAATTTGCAGATGACCATAAAACAGCACAATGACgccc    >  1:561715/1‑150 (MQ=255)
                                                                                                                                                 aaCGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGACCATAAAACAGCACAATGACgccc    >  2:3315497/1‑150 (MQ=255)
                                                                                                                                                   cGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGACCATAAAACAGCACAATGACgcccag  <  1:3822665/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGACCATAAAACAGCACAATGACGCCCAG  >  CP009273/1554440‑1554736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A