Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,554,588 G→T 46.0% A200E (GCG→GAG)  ddpB ← D‑ala‑D‑ala transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,554,5880GT46.0% 188.9 / inf 252A200E (GCG→GAG) ddpBD‑ala‑D‑ala transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (73/63);  new base T (58/58);  total (131/121)
Fisher's exact test for biased strand distribution p-value = 6.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 256 total aligned reads displayed.

CGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGACCATAAAACAGCACAATGACGCCCAGC  >  CP009273/1554439‑1554737
                                                                                                                                                     |                                                                                                                                                     
cGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACg                                                                                                                                                       <  2:2401782/150‑1 (MQ=255)
  atatACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCa                                                                                                                                                     <  2:1157458/150‑1 (MQ=255)
    ataCCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCAtt                                                                                                                                                   >  1:4135344/1‑150 (MQ=255)
        cAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTgaag                                                                                                                                               <  2:245250/150‑1 (MQ=255)
           ccGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGaagaag                                                                                                                                            <  2:1608232/150‑1 (MQ=255)
            cGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAagaaga                                                                                                                                           >  2:3968406/1‑150 (MQ=255)
             gggCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGAc                                                                                                                                          <  2:1177287/150‑1 (MQ=255)
             gggCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGACGAAGAc                                                                                                                                          >  1:2588783/1‑150 (MQ=255)
             gggCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGAc                                                                                                                                          <  1:4389121/150‑1 (MQ=255)
              ggCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACt                                                                                                                                         <  1:3085399/150‑1 (MQ=255)
                cAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTc                                                                                                                                       <  1:460463/150‑1 (MQ=255)
                cAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTc                                                                                                                                       <  1:3906302/150‑1 (MQ=255)
                 aaGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCa                                                                                                                                      <  1:554646/150‑1 (MQ=255)
                   gCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCAcc                                                                                                                                    >  1:4060738/1‑150 (MQ=255)
                     cGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGt                                                                                                                                  >  2:2360063/1‑150 (MQ=255)
                      gCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGtt                                                                                                                                 <  1:2889110/150‑1 (MQ=255)
                      gCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGtt                                                                                                                                 <  1:2148827/150‑1 (MQ=255)
                       cTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTg                                                                                                                                <  1:3713792/150‑1 (MQ=255)
                        tGGCCCGGTCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGc                                                                                                                               <  1:4376383/150‑1 (MQ=255)
                         ggCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGGTACGCATTGAAGAAGACTTCACCGTTGcc                                                                                                                              <  2:3025825/150‑1 (MQ=255)
                           cccGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCtt                                                                                                                            <  1:1061050/150‑1 (MQ=255)
                            ccGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTc                                                                                                                           <  2:1272972/150‑1 (MQ=255)
                             cGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCa                                                                                                                          <  1:4549515/150‑1 (MQ=255)
                                 cGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAagca                                                                                                                      <  1:2396786/150‑1 (MQ=255)
                                  ggTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAagcag                                                                                                                     <  1:4236000/150‑1 (MQ=255)
                                  ggTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAagcag                                                                                                                     >  2:3788316/1‑150 (MQ=255)
                                   gaaCGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAagcagc                                                                                                                    >  1:1241267/3‑150 (MQ=255)
                                     aCGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAgcgc                                                                                                                  >  2:3741070/1‑150 (MQ=255)
                                      cGAATGTAGTCTTCTCTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCa                                                                                                                 <  2:3311086/150‑1 (MQ=255)
                                      cGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCa                                                                                                                 <  2:3520210/150‑1 (MQ=255)
                                       gAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCAt                                                                                                                >  1:1734767/1‑150 (MQ=255)
                                        aaTGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATc                                                                                                               >  2:4109298/1‑150 (MQ=255)
                                                cTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGAt                                                                                                       >  2:4375601/1‑150 (MQ=255)
                                                   ctcTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCATCGTTACTGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATaaa                                                                                                    <  1:682164/148‑1 (MQ=255)
                                                       cAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGcc                                                                                                <  1:1380528/150‑1 (MQ=255)
                                                            gTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTaa                                                                                           <  2:2869070/150‑1 (MQ=255)
                                                            gTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTaa                                                                                           <  1:3520557/150‑1 (MQ=255)
                                                            gTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTaa                                                                                           >  2:201065/1‑150 (MQ=255)
                                                             ttCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAAc                                                                                          <  1:1221635/150‑1 (MQ=255)
                                                              tCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACg                                                                                         >  1:966949/1‑150 (MQ=255)
                                                               ccAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCGGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGt                                                                                        >  2:3584091/1‑150 (MQ=255)
                                                                 aGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAATAAGAATTAACCGTTGCCTTCAAGAAGCGCATCGAGAAGATAAAAGCCGGTAACGTGc                                                                                      <  2:398620/150‑1 (MQ=255)
                                                                  gCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCg                                                                                     >  2:3265055/1‑150 (MQ=255)
                                                                   cATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCAACGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAATCAGGTAACGTTCGt                                                                                    >  2:1828377/1‑150 (MQ=255)
                                                                      cGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTtgg                                                                                 >  1:2642139/1‑150 (MQ=255)
                                                                        cTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTtggtg                                                                               <  2:2776073/150‑1 (MQ=255)
                                                                         tGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTtggtgg                                                                              >  2:4305804/1‑150 (MQ=255)
                                                                          gAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGa                                                                             >  1:3383751/1‑150 (MQ=255)
                                                                          gAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGa                                                                             >  1:1217098/1‑150 (MQ=255)
                                                                          gAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGa                                                                             >  1:782003/1‑150 (MQ=255)
                                                                                      cgagcgaCAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGtca                                                                 >  1:774798/1‑150 (MQ=255)
                                                                                      cgagcgaCAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGtca                                                                 >  2:2059581/1‑150 (MQ=255)
                                                                                      cgagcgaCAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGtca                                                                 >  2:265404/1‑150 (MQ=255)
                                                                                        agcgaCAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGtcatc                                                               >  1:3041869/1‑150 (MQ=255)
                                                                                        agcgaCAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGtcatc                                                               >  1:1473811/1‑150 (MQ=255)
                                                                                          cgaCAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCaa                                                             <  1:3985889/150‑1 (MQ=255)
                                                                                          cgaCAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCaa                                                             <  1:3154356/150‑1 (MQ=255)
                                                                                            aCAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGc                                                           >  2:1128159/1‑150 (MQ=255)
                                                                                             cAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGcc                                                          >  2:3455469/1‑150 (MQ=255)
                                                                                              aaTTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCt                                                         >  1:1691137/1‑150 (MQ=255)
                                                                                              aaTTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCt                                                         >  1:2411013/1‑150 (MQ=255)
                                                                                               aTTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCtt                                                        <  1:3016034/150‑1 (MQ=255)
                                                                                                 tCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTcc                                                      >  2:4142967/1‑150 (MQ=255)
                                                                                                 tCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTcc                                                      <  1:1105591/150‑1 (MQ=255)
                                                                                                  cccAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTccg                                                     <  2:2926186/150‑1 (MQ=255)
                                                                                                  cccAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTccg                                                     <  1:2948010/150‑1 (MQ=255)
                                                                                                        tGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTccgccgccg                                               <  2:1707552/150‑1 (MQ=255)
                                                                                                          aacgaacgCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCggg                                             >  2:211473/1‑150 (MQ=255)
                                                                                                            cgaacgCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGgaa                                           <  2:3666893/150‑1 (MQ=255)
                                                                                                              aacgCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGgaaga                                         >  1:329571/1‑150 (MQ=255)
                                                                                                                cgCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAAt                                       <  1:3308837/150‑1 (MQ=255)
                                                                                                                   cAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTg                                    <  1:274431/150‑1 (MQ=255)
                                                                                                                   cAGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTg                                    >  2:4564247/1‑150 (MQ=255)
                                                                                                                    aGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGc                                   >  1:4034294/1‑150 (MQ=255)
                                                                                                                    aGCGTTAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGc                                   >  1:4034112/1‑150 (MQ=255)
                                                                                                                         tAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATg                              >  2:1032511/1‑150 (MQ=255)
                                                                                                                         tAATGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATg                              >  1:1022207/1‑150 (MQ=255)
                                                                                                                         tAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATg                              <  1:3791753/150‑1 (MQ=255)
                                                                                                                          aaTGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGa                             >  1:183102/1‑150 (MQ=255)
                                                                                                                          aaTGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGa                             >  1:2696772/1‑150 (MQ=255)
                                                                                                                          aaTGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGa                             <  1:2101288/150‑1 (MQ=255)
                                                                                                                          aaTGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGa                             >  1:1809694/1‑150 (MQ=255)
                                                                                                                          aaTGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCATTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGa                             >  1:1649086/1‑150 (MQ=255)
                                                                                                                           aTGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGAc                            <  1:264682/150‑1 (MQ=255)
                                                                                                                            tGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGAcc                           <  1:677914/150‑1 (MQ=255)
                                                                                                                            tGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGAcc                           >  1:491694/1‑150 (MQ=255)
                                                                                                                            tGCCGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGAcc                           <  1:3720605/150‑1 (MQ=255)
                                                                                                                             gCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATTACCa                          >  2:855053/1‑150 (MQ=255)
                                                                                                                              ccGGTAAGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGACCAt                         <  2:2386788/150‑1 (MQ=255)
                                                                                                                                   aaGATGAGATGTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGACCATAAAAc                    <  1:2229622/150‑1 (MQ=255)
                                                                                                                                   aaGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGACCATAAAAc                    >  2:3527800/1‑150 (MQ=255)
                                                                                                                                    agatgagatgTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGACCATAAAACa                   >  2:1606194/1‑150 (MQ=255)
                                                                                                                                      atgagatgTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGACCATAAAACAGc                 >  2:657566/1‑150 (MQ=255)
                                                                                                                                           atgTTGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGACCATAAAACAGCACAAt            <  2:3079299/150‑1 (MQ=255)
                                                                                                                                               tGCAACTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGACCATAAAACAGCACAATGACg        >  1:617947/1‑150 (MQ=255)
                                                                                                                                               tGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGACCATAAAACAGCACAATGACg        <  1:2311818/150‑1 (MQ=255)
                                                                                                                                                 cAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGACCATAAAACAGCACAATGACgcc      >  2:2281858/1‑150 (MQ=255)
                                                                                                                                                  aaCTCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGACCATAAAACAGCACAATGACgccc     <  1:2236168/150‑1 (MQ=255)
                                                                                                                                                    cGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGACCATAAAACAGCACAATGACacccag   <  2:510084/150‑1 (MQ=255)
                                                                                                                                                     gCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGACCATAAAACAGCACAATGACgcccagc  >  2:1396961/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGATATACCAGCCGGGCAAGCCGCTGGCCCGGGCGGTACGAATGTAGTCTTCACTCAATTGTTCCAGCATCGCTGAGCGGATCTGGCGAGCGACAATTCCCAGGTGAACGAACGCCAGCGTTAATGCCGGTAAGATGAGATGTTGCAACGCATTGAAGAAGACTTCACCGTTGCCTTCAAGCAGCGCATCGAGCAGATAAAAGCCGGTAACGTGCGTTGGTGGATCCAGCCAGTCATCAAGCCTTCCGCCGCCGGGAAGAATTTGCAGATGACCATAAAACAGCACAATGACGCCCAGC  >  CP009273/1554439‑1554737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A