Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,600,589 T→G 12.6% L78R (CTG→CGG)  lsrF → putative autoinducer‑2 (AI‑2) aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,600,5890TG12.6% 617.9 / 97.1 247L78R (CTG→CGG) lsrFputative autoinducer‑2 (AI‑2) aldolase
Reads supporting (aligned to +/- strand):  ref base T (118/98);  new base G (16/15);  total (134/113)
Fisher's exact test for biased strand distribution p-value = 8.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 249 total aligned reads displayed.

TGCGGTGCGCTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGCCGTGGCGTTATCGATGG  >  CP009273/1600441‑1600738
                                                                                                                                                    |                                                                                                                                                     
tGCGGTGCGCTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTg                                                                                                                                                      <  2:3731191/150‑1 (MQ=255)
  cGGTGCGCTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGtt                                                                                                                                                    >  2:2538999/1‑150 (MQ=255)
  cGGTGCGCTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGtt                                                                                                                                                    >  2:1635429/1‑150 (MQ=255)
  cGGTGCGCTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCAGGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGtt                                                                                                                                                    >  1:1011136/1‑150 (MQ=255)
   ggTGCGCTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGttt                                                                                                                                                   <  1:1766371/150‑1 (MQ=255)
       cgcTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAAc                                                                                                                                               <  1:1218365/150‑1 (MQ=255)
         cTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCTTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACAt                                                                                                                                             <  2:1917437/150‑1 (MQ=255)
         cTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACAt                                                                                                                                             >  1:2587043/1‑150 (MQ=255)
          tGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATg                                                                                                                                            >  2:818452/1‑150 (MQ=255)
          tGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATg                                                                                                                                            >  2:1367395/1‑150 (MQ=255)
          tGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATg                                                                                                                                            >  1:3406044/1‑150 (MQ=255)
            gATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGcc                                                                                                                                          >  2:1930771/1‑150 (MQ=255)
              ttGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGa                                                                                                                                        >  1:2467484/1‑150 (MQ=255)
                ggggAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCGGTTTGAACATGCCGATg                                                                                                                                      <  1:3134066/150‑1 (MQ=255)
                   gAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTAt                                                                                                                                   >  2:3479877/1‑150 (MQ=255)
                   gAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTAt                                                                                                                                   >  2:4427748/1‑150 (MQ=255)
                    aaTGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCGGTTTGAACATGCCGATGTAtt                                                                                                                                  >  1:4084996/1‑150 (MQ=255)
                    aaTGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCGGTTTGAACATGCCGATGTAtt                                                                                                                                  <  1:3528876/150‑1 (MQ=255)
                           tCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATgtgt                                                                                                                           <  2:1691028/150‑1 (MQ=255)
                            cACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCGGTTTGAACATGCCGATGTATTAATGTGTa                                                                                                                          <  2:234358/150‑1 (MQ=255)
                             aCGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTAc                                                                                                                         <  1:789905/150‑1 (MQ=255)
                                cTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTAcgcg                                                                                                                      <  1:3268341/150‑1 (MQ=255)
                                 ttATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTAcgcgc                                                                                                                     <  2:2835852/150‑1 (MQ=255)
                                  tATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTAcgcgcg                                                                                                                    <  1:2352592/150‑1 (MQ=255)
                                   aTCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCgg                                                                                                                   <  2:1886964/150‑1 (MQ=255)
                                    tCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGc                                                                                                                  >  1:4245030/1‑150 (MQ=255)
                                     cgcgGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCa                                                                                                                 <  2:3128652/150‑1 (MQ=255)
                                      gcgGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCAt                                                                                                                >  1:757691/1‑150 (MQ=255)
                                      gcgGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCAt                                                                                                                <  2:1497473/150‑1 (MQ=255)
                                         gATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCAtttt                                                                                                             >  1:775689/1‑150 (MQ=255)
                                          atatTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTg                                                                                                            <  1:997012/150‑1 (MQ=255)
                                           tatTTAATCCGAAAACGGTTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTgc                                                                                                           <  2:3603622/150‑1 (MQ=255)
                                           tatTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTgc                                                                                                           <  2:2820049/150‑1 (MQ=255)
                                               tAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAg                                                                                                       >  2:4361749/1‑150 (MQ=255)
                                                 aTCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCg                                                                                                     <  2:3441439/150‑1 (MQ=255)
                                                   ccGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCAGGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTa                                                                                                   >  2:4599245/1‑150 (MQ=255)
                                                    cGAAAACGGGTAAAACCGTGATGCTGGCTTGTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAg                                                                                                  >  2:1242428/1‑150 (MQ=255)
                                                     gAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGt                                                                                                 <  1:4349691/150‑1 (MQ=255)
                                                      aaaaCGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGtt                                                                                                >  1:2421108/1‑150 (MQ=255)
                                                      aaaaCGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGtt                                                                                                <  2:3915607/150‑1 (MQ=255)
                                                        aaCGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTcc                                                                                              >  1:4118894/1‑150 (MQ=255)
                                                            ggTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCt                                                                                          <  1:1958146/150‑1 (MQ=255)
                                                            ggTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCt                                                                                          >  2:4009273/1‑150 (MQ=255)
                                                             gTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTg                                                                                         >  2:648070/1‑150 (MQ=255)
                                                                 aaCCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGAc                                                                                     >  1:481265/1‑150 (MQ=255)
                                                                    cGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCaa                                                                                  >  2:4053785/1‑150 (MQ=255)
                                                                     gTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAAt                                                                                 <  2:2777833/150‑1 (MQ=255)
                                                                      tGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCGGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATa                                                                                <  1:3411747/150‑1 (MQ=255)
                                                                        aTGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAgg                                                                              <  1:2112752/150‑1 (MQ=255)
                                                                        aTGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAgg                                                                              >  1:2656728/1‑150 (MQ=255)
                                                                         tGCTGGCTTTTGACCATGGTTATTGTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGc                                                                             <  2:3954447/150‑1 (MQ=255)
                                                                          gCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGcc                                                                            <  2:2132480/150‑1 (MQ=255)
                                                                            tGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCgg                                                                          >  2:2977763/1‑150 (MQ=255)
                                                                            tGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCGGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCgg                                                                          >  1:1624703/1‑150 (MQ=255)
                                                                               cTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCGGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCggtgg                                                                       >  1:348924/1‑150 (MQ=255)
                                                                                 tttGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTa                                                                     >  2:3570220/1‑150 (MQ=255)
                                                                                  ttGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTAc                                                                    <  1:898909/150‑1 (MQ=255)
                                                                                   tGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACt                                                                   <  1:62583/150‑1 (MQ=255)
                                                                                   tGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACt                                                                   >  2:2500509/1‑150 (MQ=255)
                                                                                    gACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTg                                                                  >  2:3859495/1‑150 (MQ=255)
                                                                                    gACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTg                                                                  <  2:4294024/150‑1 (MQ=255)
                                                                                       cATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCgg                                                               >  2:555888/1‑150 (MQ=255)
                                                                                       cATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCgg                                                               >  2:4026208/1‑150 (MQ=255)
                                                                                       cATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCgg                                                               <  2:1655992/150‑1 (MQ=255)
                                                                                       cATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCgg                                                               <  2:1447610/150‑1 (MQ=255)
                                                                                        aTGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCggg                                                              >  2:2934312/1‑150 (MQ=255)
                                                                                          ggTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCg                                                            <  1:4048377/150‑1 (MQ=255)
                                                                                            ttATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTc                                                          <  1:2772869/150‑1 (MQ=255)
                                                                                             tATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCa                                                         <  1:2538999/150‑1 (MQ=255)
                                                                                                tttCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGt                                                      >  1:436999/1‑150 (MQ=255)
                                                                                                 ttCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTg                                                     <  1:2859977/150‑1 (MQ=255)
                                                                                                    aGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGa                                                  >  2:4275202/1‑150 (MQ=255)
                                                                                                      ggACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAAc                                                >  2:2168841/1‑150 (MQ=255)
                                                                                                      ggACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCGGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAAc                                                <  1:1586206/150‑1 (MQ=255)
                                                                                                            aCTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCGGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTAtt                                          <  2:4084996/150‑1 (MQ=255)
                                                                                                              tACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCt                                        >  1:4482037/1‑150 (MQ=255)
                                                                                                              tACCGGACTTGAACGCATTGATATAAATATCGCCCCGCGGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCt                                        <  1:1426400/150‑1 (MQ=255)
                                                                                                               aCCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTg                                       >  1:2023068/1‑150 (MQ=255)
                                                                                                                ccGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTgg                                      <  2:1318362/150‑1 (MQ=255)
                                                                                                                    aCTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGa                                  >  2:2668762/1‑150 (MQ=255)
                                                                                                                      ttGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAAt                                >  1:298291/1‑150 (MQ=255)
                                                                                                                       tGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAAtt                               >  1:1905460/1‑150 (MQ=255)
                                                                                                                       tGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAAtt                               <  1:2153661/150‑1 (MQ=255)
                                                                                                                          aCGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAg                            <  1:3773949/150‑1 (MQ=255)
                                                                                                                           cGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGt                           <  1:3799101/150‑1 (MQ=255)
                                                                                                                           cGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGt                           >  2:4183417/1‑150 (MQ=255)
                                                                                                                             cATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTaa                         >  2:4102850/1‑150 (MQ=255)
                                                                                                                             cATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTaa                         >  2:3304870/1‑150 (MQ=255)
                                                                                                                              aTTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAAt                        >  2:4513731/1‑150 (MQ=255)
                                                                                                                                tGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGa                      >  2:4279437/1‑150 (MQ=255)
                                                                                                                                   tataAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGc                   >  1:3607542/1‑150 (MQ=255)
                                                                                                                                       aaTATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGCCGTg               >  1:3077243/1‑150 (MQ=255)
                                                                                                                                       aaTATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGCCGTg               <  2:901627/150‑1 (MQ=255)
                                                                                                                                        atatCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGCCGTgg              >  1:1811853/1‑150 (MQ=255)
                                                                                                                                          atCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGCCGTGGCg            <  2:1107989/150‑1 (MQ=255)
                                                                                                                                            cGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGCCGTGGCGtt          >  2:1741895/1‑150 (MQ=255)
                                                                                                                                               cccGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGCCGTGGCGTTATc       >  1:29575/1‑150 (MQ=255)
                                                                                                                                               cccGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGCCGTGGCGTTATc       >  1:861250/1‑150 (MQ=255)
                                                                                                                                               cccGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGCCGTGGCGTTATc       >  1:1734613/1‑150 (MQ=255)
                                                                                                                                                 cGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGCCGTGGCGTTATCga     >  2:478494/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TGCGGTGCGCTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGCCGTGGCGTTATCGATGG  >  CP009273/1600441‑1600738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A