Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,600,589 T→G 38.2% L78R (CTG→CGG)  lsrF → putative autoinducer‑2 (AI‑2) aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,600,5890TG38.2% 127.3 / inf 280L78R (CTG→CGG) lsrFputative autoinducer‑2 (AI‑2) aldolase
Reads supporting (aligned to +/- strand):  ref base T (94/79);  new base G (55/52);  total (149/131)
Fisher's exact test for biased strand distribution p-value = 7.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

Only 100 of 283 total aligned reads displayed.

TGCGGTGCGCTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGCCGTGGCGTTATCGATGG  >  CP009273/1600441‑1600738
                                                                                                                                                    |                                                                                                                                                     
  cGGTGCGCTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCGGtt                                                                                                                                                    >  2:4610949/1‑150 (MQ=255)
  cGGTGCGCTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCGGtt                                                                                                                                                    <  2:1116814/150‑1 (MQ=255)
   ggTGCGCTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGttt                                                                                                                                                   >  2:2463907/1‑150 (MQ=255)
       cgcTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCGGTTTGAAc                                                                                                                                               <  2:3470450/150‑1 (MQ=255)
        gcTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACa                                                                                                                                              <  1:3957690/150‑1 (MQ=255)
        gcTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACa                                                                                                                                              >  1:4371276/1‑150 (MQ=255)
        gcTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACa                                                                                                                                              >  1:4371404/1‑150 (MQ=255)
        gcTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCGGTTTGAACa                                                                                                                                              >  1:141772/1‑150 (MQ=255)
        gcTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTGTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACa                                                                                                                                              >  1:3426472/1‑150 (MQ=255)
          tGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATg                                                                                                                                            >  1:267376/1‑150 (MQ=255)
          tGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCGGTTTGAACATg                                                                                                                                            >  1:53306/1‑150 (MQ=255)
           ggATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCGGTTTGAACATGc                                                                                                                                           >  1:59002/1‑150 (MQ=255)
           ggATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGATGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGc                                                                                                                                           <  1:3606680/150‑1 (MQ=255)
            gATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGcc                                                                                                                                          <  2:1723013/150‑1 (MQ=255)
            gATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCGGTTTGAACATGcc                                                                                                                                          <  2:2031722/150‑1 (MQ=255)
                ggggAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATg                                                                                                                                      >  2:479516/1‑150 (MQ=255)
                  ggAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTCCCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTa                                                                                                                                    >  1:124097/1‑150 (MQ=255)
                   gAATGCAGTCACTCTTATCGCGGATATTTAATCCGAAAACGTGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCGGTTTGAACATGCCGATGTAt                                                                                                                                   <  1:4479184/150‑1 (MQ=255)
                    aaTGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTAtt                                                                                                                                  >  1:4607946/1‑150 (MQ=255)
                    aaTGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTAtt                                                                                                                                  >  1:3738755/1‑150 (MQ=255)
                     aTGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCGGTTTGAACATGCCGATGTATTa                                                                                                                                 >  2:4421293/1‑150 (MQ=255)
                           tCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATgtgt                                                                                                                           <  1:4211459/150‑1 (MQ=255)
                             aCGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTAc                                                                                                                         >  1:1873874/1‑150 (MQ=255)
                                cTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCGGTTTGAACATGCCGATGTATTAATGTGTAcgcg                                                                                                                      >  2:969413/1‑150 (MQ=255)
                                 ttATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTAcgcgc                                                                                                                     >  2:1256587/1‑150 (MQ=255)
                                    tCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCGGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGc                                                                                                                  <  1:2223479/150‑1 (MQ=255)
                                     cgcgGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCa                                                                                                                 <  2:4251835/150‑1 (MQ=255)
                                       cgGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCAtt                                                                                                               >  1:4406758/1‑150 (MQ=255)
                                        ggATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCAttt                                                                                                              >  1:3984483/1‑150 (MQ=255)
                                          atatTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTg                                                                                                            <  1:3095351/150‑1 (MQ=255)
                                              ttAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCa                                                                                                        >  1:233723/1‑150 (MQ=255)
                                              ttAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCGGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCa                                                                                                        <  1:4296123/150‑1 (MQ=255)
                                               tAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAg                                                                                                       <  1:2765899/150‑1 (MQ=255)
                                                aaTCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCGGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGc                                                                                                      >  1:1722158/1‑150 (MQ=255)
                                                    cGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCGGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAg                                                                                                  <  2:3606098/150‑1 (MQ=255)
                                                        aaCGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTcc                                                                                              >  2:1884822/1‑150 (MQ=255)
                                                        aaCGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCGGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTcc                                                                                              >  1:1089647/1‑150 (MQ=255)
                                                         aCGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTccc                                                                                             >  1:1629375/1‑150 (MQ=255)
                                                           gggTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTccccc                                                                                           >  1:325540/1‑150 (MQ=255)
                                                            ggTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCt                                                                                          >  2:2249516/1‑150 (MQ=255)
                                                            ggTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCt                                                                                          <  2:2963703/150‑1 (MQ=255)
                                                            ggTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCt                                                                                          >  2:924370/1‑150 (MQ=255)
                                                             gTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTg                                                                                         <  1:2717391/150‑1 (MQ=255)
                                                               aaaaCCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCGGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCg                                                                                       >  1:390234/1‑150 (MQ=255)
                                                                aaaCCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCGGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGa                                                                                      >  2:2414487/1‑150 (MQ=255)
                                                                 aaCCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGAc                                                                                     >  1:3336432/1‑150 (MQ=255)
                                                                      tGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATa                                                                                <  2:3226144/150‑1 (MQ=255)
                                                                      tGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATa                                                                                >  1:989908/1‑150 (MQ=255)
                                                                      tGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATa                                                                                <  2:3749042/150‑1 (MQ=255)
                                                                          gCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGcc                                                                            <  2:596803/150‑1 (MQ=255)
                                                                          gCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGcc                                                                            <  2:3423562/150‑1 (MQ=255)
                                                                          gCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCGGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGcc                                                                            >  2:2197935/1‑150 (MQ=255)
                                                                            tGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCgg                                                                          >  1:932789/1‑150 (MQ=255)
                                                                               cTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCggtgg                                                                       >  1:2967676/1‑150 (MQ=255)
                                                                                ttttGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCggtggt                                                                      >  1:129050/1‑150 (MQ=255)
                                                                                   tGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACt                                                                   <  1:1160518/150‑1 (MQ=255)
                                                                                     aCCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGc                                                                 <  1:2009219/150‑1 (MQ=255)
                                                                                     aCCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCGGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGc                                                                 <  1:3410745/150‑1 (MQ=255)
                                                                                      ccATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCGGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCg                                                                <  2:3012323/150‑1 (MQ=255)
                                                                                         tGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCGGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGc                                                             <  2:2784800/150‑1 (MQ=255)
                                                                                          ggTTATTTTCAGGGACCGACTCCCGGACTTGAACGCATTGATATAAATATCGCCCCGCGGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCg                                                            >  1:4413348/1‑150 (MQ=255)
                                                                                          ggTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCg                                                            <  1:1012677/150‑1 (MQ=255)
                                                                                              aTTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAg                                                        <  1:3929180/150‑1 (MQ=255)
                                                                                                    aGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGa                                                  >  2:181197/1‑150 (MQ=255)
                                                                                                    aGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGa                                                  >  1:664504/1‑150 (MQ=255)
                                                                                                    aGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCAGTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGa                                                  >  2:3955768/1‑150 (MQ=255)
                                                                                                      ggACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCGGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAAc                                                <  1:2581758/150‑1 (MQ=255)
                                                                                                       gACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAAct                                               <  2:1174254/150‑1 (MQ=255)
                                                                                                       gACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAAct                                               <  2:265826/150‑1 (MQ=255)
                                                                                                          cGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCGGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGGGGTACTGCGGGCGTCAGGTGCGAACTCTa                                            >  2:2125102/1‑150 (MQ=255)
                                                                                                           gACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCGGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTAt                                           <  2:2670327/150‑1 (MQ=255)
                                                                                                            aCTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGCACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTAtt                                          >  1:3600077/1‑150 (MQ=255)
                                                                                                              tACCGGACTTGAACGCATTGATATAAATATCGCCCCGCGGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCt                                        >  2:3893256/1‑150 (MQ=255)
                                                                                                               aCCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTg                                       <  2:1914922/150‑1 (MQ=255)
                                                                                                               aCCGGACTTGAACGCATTGATATAAATATCGCCCCGCGGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTg                                       >  1:2391887/1‑150 (MQ=255)
                                                                                                                ccGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTgg                                      <  2:3222421/150‑1 (MQ=255)
                                                                                                                 cGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGc                                     >  1:3067397/1‑150 (MQ=255)
                                                                                                                 cGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGc                                     >  1:4552961/1‑150 (MQ=255)
                                                                                                                  ggACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCg                                    <  2:2862156/150‑1 (MQ=255)
                                                                                                                     cTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGaa                                 <  1:2921756/150‑1 (MQ=255)
                                                                                                                      ttGAACGCTTTGATATAAATTTCGGCCCGCGGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCTCAGCGTTGTTCCCCCTGCGACCAATAGGCCGGTGTTACTGCGGGCGTCTGGTGCGAACTCTATTCTGGCGGAAt                                <  2:285098/150‑1 (MQ=255)
                                                                                                                      ttGAACGCATTGATATAAATATCGCCCCGCGGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAAt                                <  1:2013999/150‑1 (MQ=255)
                                                                                                                      ttGAACGCATTGATATAAATATCGCCCCGCGGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAAt                                <  1:1121447/150‑1 (MQ=255)
                                                                                                                        gAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTa                              >  2:605722/1‑150 (MQ=255)
                                                                                                                        gAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTa                              <  1:469877/150‑1 (MQ=255)
                                                                                                                             cATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTaa                         >  1:1837484/1‑150 (MQ=255)
                                                                                                                             cATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGACAGGTGCGAACTCTATTCTGGCGGAATTAAGaaa                         >  1:482471/1‑150 (MQ=255)
                                                                                                                               ttGATATAAATATCGCCCCGCGGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATg                       >  2:2987089/1‑150 (MQ=255)
                                                                                                                                   tataAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGc                   <  1:1742952/150‑1 (MQ=255)
                                                                                                                                   tataAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGc                   >  2:66494/1‑150 (MQ=255)
                                                                                                                                     taAATATCGCCCCGCGGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGCCg                 <  1:2125373/150‑1 (MQ=255)
                                                                                                                                      aaaTATCGCCCCGCGGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGCCGt                >  2:2506138/1‑150 (MQ=255)
                                                                                                                                       aaTATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGCCGTg               <  2:4060437/150‑1 (MQ=255)
                                                                                                                                         tatCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGCCGTGGc             >  2:3569499/1‑150 (MQ=255)
                                                                                                                                          atCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGCCGTGGCg            >  2:1534856/1‑150 (MQ=255)
                                                                                                                                             gCCCCGCGGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGCCGTGGCGTTa         >  1:2540073/1‑150 (MQ=255)
                                                                                                                                              ccccGCGGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGCCGTGGCGTTAt        <  1:3861388/150‑1 (MQ=255)
                                                                                                                                                ccGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGCCGTGGCGTTATCg      <  2:334693/150‑1 (MQ=255)
                                                                                                                                                    tGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGCCGTGGCGTTATCgatgg  >  2:2689413/1‑150 (MQ=255)
                                                                                                                                                    tGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGCCGTGGCGTTATCgatgg  >  2:4458915/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TGCGGTGCGCTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGCGAACTCTATTCTGGCGGAATTAAGTAATGAAGCCGTGGCGTTATCGATGG  >  CP009273/1600441‑1600738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A