Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,281,560 T→A 17.5% pseudogene (834/2525 nt) yejO ← pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,281,5600TA17.5% 718.8 / 136.5 269pseudogene (834/2525 nt)yejOpseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system
Reads supporting (aligned to +/- strand):  ref base T (128/94);  new base A (20/27);  total (148/121)
Fisher's exact test for biased strand distribution p-value = 7.54e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 270 total aligned reads displayed.

ACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAGGAATACGTGATACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCG  >  CP009273/2281412‑2281709
                                                                                                                                                    |                                                                                                                                                     
aCGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAGGAATACGTGATACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTg                                                                                                                                                      <  2:6009/150‑1 (MQ=255)
  gTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAGGAATACGTGATACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGc                                                                                                                                                    <  1:3820062/150‑1 (MQ=255)
  gTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAGGAATACGTGATACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGc                                                                                                                                                    >  2:1033645/1‑150 (MQ=255)
     aGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAGGAATACGTGATACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGAGGCATg                                                                                                                                                 >  1:4328113/1‑150 (MQ=255)
      gcgcGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAGGAATACGTGATACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGc                                                                                                                                                >  1:456763/1‑150 (MQ=255)
         cgGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAGGAATACGTGATACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTa                                                                                                                                             >  1:2606337/1‑150 (MQ=255)
         cgGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAGGAATACGTGATACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTa                                                                                                                                             >  1:706951/1‑150 (MQ=255)
         cgGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAGGAATACGTGATACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGAGGCATGCTTa                                                                                                                                             >  1:2496179/1‑150 (MQ=255)
           gCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAGGAATACGTGATACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATc                                                                                                                                           >  2:4245637/1‑150 (MQ=255)
             cATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAGGAATACGTGATACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGAGGCATGCTTATCaa                                                                                                                                         >  2:519733/1‑150 (MQ=255)
                   tgATAACCATATTTTCAGAGATGGCTTTTGAGGAATACGTGATACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAg                                                                                                                                   >  2:3124807/1‑150 (MQ=255)
                    gATAACCATATTTTCAGAGATGGCTTTTGAGGAATACGTGATACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGt                                                                                                                                  <  2:238086/150‑1 (MQ=255)
                     aTAACCATATTTTCAGAGATGGCTTTTGAGGAATACGTGATACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTa                                                                                                                                 >  1:4082821/1‑150 (MQ=255)
                       aaCCATATTTTCAGAGATGGCTTTTGAGGAATACGTGATACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATc                                                                                                                               >  1:2608077/1‑150 (MQ=255)
                         ccATATTTTCAGAGATGGCTTTTGAGGAATACGTGATACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGAGGCATGCTTATCAAGGATAGTATCaa                                                                                                                             <  1:2976884/150‑1 (MQ=255)
                           atatTTTCAGAGATGGCTTTTGAGGAATACGTGATACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATg                                                                                                                           >  1:4361855/1‑150 (MQ=255)
                            tatTTTCAGAGATGGCTTTTGAGGAATACGTGATACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATgg                                                                                                                          >  1:4240029/1‑150 (MQ=255)
                             atTTTCAGAGATGGCTTTTGAGGAATACGTGATACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGc                                                                                                                         >  2:1507211/1‑150 (MQ=255)
                             atTTTCAGAGATGGCTTTTGAGGAATACGTGATACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGAGGCATGCTTATCAAGGATAGTATCAATGGc                                                                                                                         >  2:1923793/1‑150 (MQ=255)
                                  cAGAGATGGCTTTTGAGGAATACGTGATACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCg                                                                                                                    >  1:728338/1‑150 (MQ=255)
                                  cAGAGATGGCTTTTGAGGAATACGTGATACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCg                                                                                                                    <  1:3686340/150‑1 (MQ=255)
                                  cAGAGATGGCTTTTGAGGAATACGTGATACTTCCATTCTGATAGGACCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGAGGCATGCTTATCAAGGATAGTATCAATGGCCTGCg                                                                                                                    <  2:1364903/150‑1 (MQ=255)
                                     agaTGGCTTTTGAGGAATACGTGATACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCgtgt                                                                                                                 <  1:1905777/150‑1 (MQ=255)
                                     agaTGGCTTTTGAGGAATACGTGATACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGAGGCATGCTTATCAAGGATAGTATCAATGGCCTGCgtgt                                                                                                                 >  1:3942142/1‑150 (MQ=255)
                                      gaTGGCTTTTGAGGAATACGTGATACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGtc                                                                                                                <  1:3138123/150‑1 (MQ=255)
                                       aTGGCTTTTGAGGAATACGTGATACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGAGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGtcc                                                                                                               >  1:3405806/1‑150 (MQ=255)
                                        tGGCTTTTGAGGAATACGTGATACTTCCATTCTGATATGATCGACCGAGATCATAGTCCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGtcct                                                                                                              <  1:3292318/150‑1 (MQ=255)
                                          gCTTTTGAGGAATACGTGATACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGtcctcc                                                                                                            >  1:3346377/1‑150 (MQ=255)
                                             tttGAGGAATACGTGATACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATa                                                                                                         <  1:3046507/150‑1 (MQ=255)
                                               tGAGGAATACGTGATACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGAGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAAc                                                                                                       <  1:4410843/150‑1 (MQ=255)
                                                gAGGAATACGTGATACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACa                                                                                                      >  2:3214956/1‑150 (MQ=255)
                                                   gAATACGTGATACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGAGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATg                                                                                                   <  2:1440693/150‑1 (MQ=255)
                                                     aTACGTGATACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAg                                                                                                 <  2:1735582/150‑1 (MQ=255)
                                                     aTACGTGATACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGAGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACACTGAg                                                                                                 <  1:1122681/150‑1 (MQ=255)
                                                      tACGTGATACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGc                                                                                                <  1:4096790/150‑1 (MQ=255)
                                                      tACGTGATACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGc                                                                                                >  1:3154519/1‑150 (MQ=255)
                                                        cGTGATACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGAGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGccc                                                                                              >  1:2738517/1‑150 (MQ=255)
                                                         gTGATACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCg                                                                                             >  2:4304905/1‑150 (MQ=255)
                                                             tACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAAc                                                                                         <  2:729925/150‑1 (MQ=255)
                                                               cTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGAGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACca                                                                                       <  1:4426869/150‑1 (MQ=255)
                                                                ttCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACcat                                                                                      <  2:2042343/150‑1 (MQ=255)
                                                                 tCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACcatc                                                                                     >  1:3718686/1‑150 (MQ=255)
                                                                 tCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACcatc                                                                                     >  1:5080/1‑150 (MQ=255)
                                                                    aTTCTGATATGATAGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATTCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCatcatc                                                                                  >  1:2103760/1‑150 (MQ=255)
                                                                       cTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAg                                                                               >  1:1075413/1‑150 (MQ=255)
                                                                        tGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGt                                                                              >  1:1841166/1‑150 (MQ=255)
                                                                        tGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGt                                                                              >  2:3088113/1‑150 (MQ=255)
                                                                         gATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGtt                                                                             <  1:2546650/150‑1 (MQ=255)
                                                                              gATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATaa                                                                        >  1:3527546/1‑150 (MQ=255)
                                                                                tCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAAtt                                                                      <  2:132916/150‑1 (MQ=255)
                                                                                 cGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAAttt                                                                     <  1:1263203/150‑1 (MQ=255)
                                                                                 cGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGAGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGACCCATCATCCAGTTCATAAttt                                                                     >  2:2879833/1‑150 (MQ=255)
                                                                                  gACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGAGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAAtttt                                                                    >  1:50945/1‑150 (MQ=255)
                                                                                    ccGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAAtttttt                                                                  >  2:4572439/1‑150 (MQ=255)
                                                                                     cGAGATCAAAAACCGCATTTGCCTGCACTTTCGTACCAGAATCATATCCCAGCGATTGCATAGTGTCATACTTATAAATTAAAGTATCAATGTCCTGCTTGTCCTCCATAACAATAAGCACGGAACAATCATACAGTTCATAATTTATTg                                                                 >  2:1590564/1‑150 (MQ=255)
                                                                                      gagaTCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGa                                                                >  2:2748735/1‑150 (MQ=255)
                                                                                      gagaTCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGa                                                                >  1:1050856/1‑150 (MQ=255)
                                                                                       agaTCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGac                                                               >  2:2523544/1‑150 (MQ=255)
                                                                                         aTCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGacac                                                             >  1:1492256/1‑150 (MQ=255)
                                                                                                 ccGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCtt                                                     <  2:3378231/150‑1 (MQ=255)
                                                                                                  cGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCttt                                                    <  2:3768877/150‑1 (MQ=255)
                                                                                                   gCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCtttt                                                   <  2:4448151/150‑1 (MQ=255)
                                                                                                   gCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGAGTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACAATCATACAGTTCATAATTTTTTGACACACCATCtttt                                                   <  2:615793/150‑1 (MQ=255)
                                                                                                    cATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTta                                                  <  2:1790606/150‑1 (MQ=255)
                                                                                                       ttGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATAc                                               >  1:840542/1‑150 (MQ=255)
                                                                                                          ccTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTaa                                            >  1:226971/1‑150 (MQ=255)
                                                                                                          ccTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTaa                                            >  1:1711180/1‑150 (MQ=255)
                                                                                                           cTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTaaa                                           <  2:4411977/150‑1 (MQ=255)
                                                                                                           cTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTaaa                                           <  2:352839/150‑1 (MQ=255)
                                                                                                             gCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAAtt                                         <  2:1853993/150‑1 (MQ=255)
                                                                                                              aaatttAGTACCAGTATCCTTTCCAAGCGATTGCATAGTGGCATGCTTTTATTGGATAGTATCAATGGCCTGCGTGTCCTCCATAACATTGATCCAGGAACCATCATCCAGTTAATAATTTTTTGACACAACATATTTTTTACTTAATTg                                        <  2:2136048/147‑1 (MQ=255)
                                                                                                                cTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGc                                      >  1:644614/1‑150 (MQ=255)
                                                                                                                cTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGc                                      <  2:2330337/150‑1 (MQ=255)
                                                                                                                  ttCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCt                                    <  2:3472165/150‑1 (MQ=255)
                                                                                                                  ttCGTACCAGTATCCTTTCCCAGCGATTGCATAGAGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCt                                    <  2:1672670/150‑1 (MQ=255)
                                                                                                                     gTACCAGTATCCTTTCCCAGCGATTGCATAGAGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTa                                 >  1:1038848/1‑150 (MQ=255)
                                                                                                                      tACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTAc                                <  2:2512203/150‑1 (MQ=255)
                                                                                                                       aCCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTTCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACt                               <  1:413590/150‑1 (MQ=255)
                                                                                                                       aCCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACt                               >  2:189840/1‑150 (MQ=255)
                                                                                                                           gTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGttt                           >  2:4173218/1‑150 (MQ=255)
                                                                                                                            tATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTg                          <  2:4543830/150‑1 (MQ=255)
                                                                                                                            tATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTg                          >  2:798301/1‑150 (MQ=255)
                                                                                                                              tCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGa                        <  2:4361855/150‑1 (MQ=255)
                                                                                                                               ccTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGAc                       <  1:1533406/150‑1 (MQ=255)
                                                                                                                                 tttCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACca                     <  1:1157177/150‑1 (MQ=255)
                                                                                                                                   tCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACcact                   <  2:3069476/150‑1 (MQ=255)
                                                                                                                                   tCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACcact                   <  2:1152523/150‑1 (MQ=255)
                                                                                                                                         cGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACcactcactgc             >  2:4046134/1‑148 (MQ=255)
                                                                                                                                         cGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTc             >  2:3314912/1‑150 (MQ=255)
                                                                                                                                         cGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTc             >  2:2854137/1‑150 (MQ=255)
                                                                                                                                          gATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTcc            >  1:3090655/1‑150 (MQ=255)
                                                                                                                                             tGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGTCCTTTACTGTTTGGACCACTCACTTCCAgc         >  1:2318128/1‑150 (MQ=255)
                                                                                                                                              gCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAgcg        >  1:2815925/1‑150 (MQ=255)
                                                                                                                                              gCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAgcg        >  2:3938635/1‑150 (MQ=255)
                                                                                                                                               cATAGAGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAgcgc       >  1:3746695/1‑150 (MQ=255)
                                                                                                                                                 tAGAGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCAt     <  2:3405806/150‑1 (MQ=255)
                                                                                                                                                  aGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCAtt    >  2:2555154/1‑150 (MQ=255)
                                                                                                                                                  aGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCAtt    <  2:1626254/150‑1 (MQ=255)
                                                                                                                                                   gTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCCGCGCATTc   <  2:2606337/150‑1 (MQ=255)
                                                                                                                                                   gTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTc   <  2:2119325/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAGGAATACGTGATACTTCCATTCTGATATGATCGACCGAGATCATATACCGCATTTGCCTGCACTTTCGTACCAGTATCCTTTCCCAGCGATTGCATAGTGGCATGCTTATCAAGGATAGTATCAATGGCCTGCGTGTCCTCCATAACAATGAGCCCGGAACCATCATCCAGTTCATAATTTTTTGACACACCATCTTTTATACTAAATTGGCCTTTACTGTTTGGACCACTCACTTCCAGCGCATTCG  >  CP009273/2281412‑2281709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A