Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,797,907 G→T 35.8% V190V (GTC→GTA)  waaS ← lipopolysaccharide core biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,797,9070GT35.8% 344.5 / 214.6 218V190V (GTC→GTA) waaSlipopolysaccharide core biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base G (77/63);  new base T (37/41);  total (114/104)
Fisher's exact test for biased strand distribution p-value = 3.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

Only 100 of 218 total aligned reads displayed.

CCTCAAGTATACGAAGAGTCCAGCCAGATTGGTTTTCTTTGGTTATATCAACAATTATATTAGCATTTAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGAAAAAACATATTGGCTGGCTGTTATTTTTGTCAGATAAACTATAATTTCTAATTTCAT  >  CP009273/3797759‑3798055
                                                                                                                                                    |                                                                                                                                                    
ccTCAAGTATACGAAGAGTCCAGCCAGATTGGTTTTCTTTGGTTATATCAACAATTATATTAGCATTTAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGa                                                                                                                                                     <  1:3403183/150‑1 (MQ=255)
    aaGTATACGAAGAGTCCAGCCAGATTGGTTTTCTTTGGTTATATCAACAATTATATTAGCATTTAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGacaac                                                                                                                                                 >  2:3229314/1‑150 (MQ=255)
     aGTATACGAAGAGTCCAGCCAGATTGGTTTTCTTTGGTTATATCAACAATTATATTAGCATTTAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTTacaaca                                                                                                                                                >  1:3395037/1‑150 (MQ=255)
     aGTATACGAAGAGTCCAGCCAGATTGGTTTTCTTTGGTTATATCAACAATTATATTAGCATTTAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTTacaaca                                                                                                                                                >  1:3394948/1‑150 (MQ=255)
     aGTATACGAAGAGTCCAGCCAGATTGGTTTTCTTTGGTTATATCAACAATTATATTAGCATTTAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGacaaca                                                                                                                                                >  1:636053/1‑150 (MQ=255)
     aGTATACGAAGAGTCCAGCCAGATTGGTTTTCTTTGGTTATATCAACAATTATATTAGCATTTAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGacaaca                                                                                                                                                >  1:1809316/1‑150 (MQ=255)
       tataCGAAGAGTCCAGCCAGATTGGTTTTCTTTGGTTATATCAACAATTATATTAGCATTTAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACAtt                                                                                                                                              >  2:3751016/1‑150 (MQ=255)
            gAAGAGTCCAGCCAGATTGGTTTTCTTTGGTTATATCAACAATTATATTAGCATTTAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAAt                                                                                                                                         >  1:2242031/1‑150 (MQ=255)
              agagTCCAGCCAGATTGGTTTTCTTTTGTTATATCAACAATTATATTAGAATTTAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTTACAACATTAAAATCt                                                                                                                                       <  2:3121057/150‑1 (MQ=255)
               gagTCCAGCCAGATTGGTTTTCTTTGGTTATATCAACAATTATATTAGCATTTAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTTACAACATTAAAATCTa                                                                                                                                      <  2:3492671/150‑1 (MQ=255)
                agTCCAGCCAGATTGGTTTTCTTTGGTTATATCAACAATTATATTAGCATTTAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAg                                                                                                                                     >  1:4105152/1‑150 (MQ=255)
                    cagccagATTGGTTTTCTTTGGTTATATCAACAATTATATTAGCATTTAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTa                                                                                                                                 <  2:2570622/150‑1 (MQ=255)
                       ccagATTGGTTTTCTTTGGTTATATCAACAATTATATTAGCATTTAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACAt                                                                                                                              >  1:1027031/1‑150 (MQ=255)
                        cagATTGGTTTTCTTTGGTTATATCAACAATTATATTAGCATTTAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTTACAACATTAAAATCTAGCTTACATc                                                                                                                             >  1:4017876/1‑150 (MQ=255)
                        cagATTGGTTTTCTTTGGTTATATCAACAATTATATTAGCATTTAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTTACAACATTAAAATCTAGCTTACATc                                                                                                                             >  1:3486357/1‑150 (MQ=255)
                          gATTGGTTTTCTTTGGTTATATCAACAATTATATTAGCATTTAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCt                                                                                                                           <  2:631924/150‑1 (MQ=255)
                          gATTGGTTTTCTTTGGTTATATCAACAATTATATTAGCATTTAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCt                                                                                                                           <  2:168040/150‑1 (MQ=255)
                           aTTGGTTTTCTTTGGTTATATCAACAATTATATTAGCATTTAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTa                                                                                                                          >  2:4416486/1‑150 (MQ=255)
                            ttGGTTTTCTTTGGTTATATCAACAATTATATTAGCATTTAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAg                                                                                                                         >  1:2190523/1‑150 (MQ=255)
                               gTTTTCTTTGGTTATATCAACAATTATATTAGCATTTAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAgtgt                                                                                                                      >  2:1672147/1‑150 (MQ=255)
                               gTTTTCTTTGGTTATATCAACAATTATATTAGCATTTAATGTTCTTCTAATATTATCTTCATAAGATATTTGTTTTTCAATCAAATACTTTTAAGTCTTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTAAATACTAgtgt                                                                                                                      >  1:3098951/1‑150 (MQ=255)
                                ttttCTTTGGTTATATCAACAATTATATTAGCATTTAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGtgtt                                                                                                                     >  2:3657315/1‑150 (MQ=255)
                                 tttCTTTGGTTATATCAACAATTATATTAGCATTTAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGtgttg                                                                                                                    >  2:4089603/1‑150 (MQ=255)
                                 tttCTTTGGTTATATCAACAATTATATTAGCATTTAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGtgttg                                                                                                                    >  2:4089717/1‑150 (MQ=255)
                                  ttCTTTGGTTATATCAACAATTATATTAGCATTTAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGtgttgt                                                                                                                   >  2:2778825/1‑150 (MQ=255)
                                   tCTTTGGTTATATCAACAATTATATTAGCATTTAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTgttgtt                                                                                                                  <  1:2281224/150‑1 (MQ=255)
                                        ggTTATATCAACAATTATATTAGCATTTAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTTACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAAtct                                                                                                             <  1:4442186/150‑1 (MQ=255)
                                           tataTCAACAATTATATTAGCATTTAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAAtctctc                                                                                                          >  2:3293807/1‑150 (MQ=255)
                                           tataTCAACAATTATATTAGCATTTAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAAtctctc                                                                                                          <  1:2545110/150‑1 (MQ=255)
                                             tatCAACAATTATATTAGCATTTAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTTACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCgg                                                                                                        >  2:3284678/1‑150 (MQ=255)
                                              atCAACAATTATATTAGCATTTAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTTACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGc                                                                                                       >  1:4175968/1‑150 (MQ=255)
                                                   caaTTATATTAGCATTTAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTTACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACt                                                                                                  <  1:2400537/150‑1 (MQ=255)
                                                      ttATATTAGCATTTAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTTACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCat                                                                                               <  1:1634874/150‑1 (MQ=255)
                                                          atTAGCATTTAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAGGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCattaat                                                                                           >  1:578517/1‑150 (MQ=255)
                                                          atTAGCATTTAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTTACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCattaat                                                                                           >  1:590679/1‑150 (MQ=255)
                                                                aTTTAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTg                                                                                     >  2:1634469/1‑150 (MQ=255)
                                                                aTTTAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTg                                                                                     <  2:3331786/150‑1 (MQ=255)
                                                                 tttAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGa                                                                                    >  2:3911711/1‑150 (MQ=255)
                                                                 tttAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGa                                                                                    >  2:1776512/1‑150 (MQ=255)
                                                                  ttAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTTaa                                                                                   >  1:791385/1‑150 (MQ=255)
                                                                     aTGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTTACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGAc                                                                                <  1:354563/150‑1 (MQ=255)
                                                                      tGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACg                                                                               <  1:4324156/150‑1 (MQ=255)
                                                                      tGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACg                                                                               <  2:1187348/150‑1 (MQ=255)
                                                                       gTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGc                                                                              >  1:2970041/1‑150 (MQ=255)
                                                                          cttctAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTTACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCt                                                                           >  1:2876069/1‑150 (MQ=255)
                                                                          cttctAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCt                                                                           <  1:1727510/150‑1 (MQ=255)
                                                                            tctAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCttt                                                                         >  1:1321535/1‑150 (MQ=255)
                                                                             ctAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTa                                                                        <  1:337571/150‑1 (MQ=255)
                                                                             ctAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTa                                                                        <  1:2656325/150‑1 (MQ=255)
                                                                              tAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTAt                                                                       >  1:4547021/1‑150 (MQ=255)
                                                                               aaTATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTTACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATc                                                                      >  1:3601977/1‑150 (MQ=255)
                                                                               aaTATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATc                                                                      <  1:815069/150‑1 (MQ=255)
                                                                                atatTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTTACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCa                                                                     >  1:1189745/1‑150 (MQ=255)
                                                                                atatTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTTACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCa                                                                     >  1:2549834/1‑150 (MQ=255)
                                                                                atatTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCa                                                                     >  2:2469922/1‑150 (MQ=255)
                                                                                atatTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCa                                                                     >  1:3763769/1‑150 (MQ=255)
                                                                                     ctctTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGAcc                                                                >  2:2800492/1‑150 (MQ=255)
                                                                                        ttCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAg                                                             <  2:4587710/150‑1 (MQ=255)
                                                                                          cATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTTACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGa                                                           >  2:1514983/1‑150 (MQ=255)
                                                                                          cATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTTACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGa                                                           <  1:1513615/150‑1 (MQ=255)
                                                                                              aGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTTACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGaaaaa                                                       <  2:2228488/150‑1 (MQ=255)
                                                                                              aGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGaaaaa                                                       >  2:132711/1‑150 (MQ=255)
                                                                                               gATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGaaaaaa                                                      <  1:1045674/150‑1 (MQ=255)
                                                                                                 tatTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTTACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGAAAAAACa                                                    <  2:3064273/150‑1 (MQ=255)
                                                                                                 tatTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTTACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGAAAAAACa                                                    >  2:73445/1‑150 (MQ=255)
                                                                                                 tatTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGAAAAAACa                                                    >  2:1418717/1‑150 (MQ=255)
                                                                                                  atTTGTTTTTCAATCAGATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTATTTATTTGAAGACGCCCTTTATCACGACCAAGGAAAAAACat                                                   >  2:1003366/1‑150 (MQ=255)
                                                                                                    ttGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGAAAAAACatat                                                 <  1:1002604/150‑1 (MQ=255)
                                                                                                     tGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTTTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGAAAAAACATAtt                                                <  2:237455/150‑1 (MQ=255)
                                                                                                     tGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGAAAAAACATAtt                                                <  1:3793013/150‑1 (MQ=255)
                                                                                                          ttCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGAAAAAACATATtggctg                                           >  2:3791657/1‑150 (MQ=255)
                                                                                                           tcaatcaaATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGAAAAAACATATtggctgg                                          >  1:4473283/1‑150 (MQ=255)
                                                                                                             aatcaaATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGAAAAAACATATTggctggct                                        >  1:217423/1‑150 (MQ=255)
                                                                                                              atcaaATACTTTGAAGTCGTAGATGATGTCTTATCTTTTACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGAAAAAACATATTGgctggctg                                       <  2:3477008/150‑1 (MQ=255)
                                                                                                              atcaaATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGAAAAAACATATTGgctggctg                                       <  1:4459603/150‑1 (MQ=255)
                                                                                                                  aaTACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGAAAAAACATATTGGCTGGCTGTTAt                                   >  1:4452549/1‑150 (MQ=255)
                                                                                                                  aaTACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGAAAAAACATATTGGCTGGCTGTTAt                                   >  2:1535130/1‑150 (MQ=255)
                                                                                                                   aTACTTTGAAGTCGTAGATGATGTCTTATCTTTTACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGAAAAAACATATTGGCTGGCTGTTAtt                                  >  1:718277/1‑150 (MQ=255)
                                                                                                                    tACTTTGAAGTCGTAGATGATGTCTTATCTTTTACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGAAAAAACATATTGGCTGGCTGTTAttt                                 <  1:4203653/150‑1 (MQ=255)
                                                                                                                    tACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGAAAAAACATATTGGCTGGCTGTTAttt                                 >  1:1130538/1‑150 (MQ=255)
                                                                                                                       tttGAAGTCGTAGATGATGTCTTATCTTTTACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAATACGCCCTTTATCACGACCAAGGAAAAAACATATTGGCTGGCTGTTATTTTTg                              <  2:4263538/150‑1 (MQ=255)
                                                                                                                        ttGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGAAAAAACATATTGGCTGGCTGTTATTTTTGt                             <  2:1982978/150‑1 (MQ=255)
                                                                                                                         tGAAGTCGTAGATGATGTCTTATCTTTTACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGAAAAAACATATTGGCTGGCTGTTATTTTTGTc                            >  2:3322769/1‑150 (MQ=255)
                                                                                                                         tGAAGTCGTAGATGATGTCTTATCTTTTACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGAAAAAACATATTGGCTGGCTGTTATTTTTGTc                            >  2:3821622/1‑150 (MQ=255)
                                                                                                                           aaGTCGTAGATGATGTCTTATCTTTTACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGAAAAAACATATTGGCTGGCTGTTATTTTTGTCAg                          >  1:3233678/1‑150 (MQ=255)
                                                                                                                           aaGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGAAAAAACATATTGGCTGGCTGTTATTTTTGTCAg                          >  2:752498/1‑150 (MQ=255)
                                                                                                                           aaGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGAAAAAACATATTGGCTGGCTGTTATTTTTGTCAg                          <  2:2447833/150‑1 (MQ=255)
                                                                                                                              tCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGAAAAAACATATTGGCTGGCTGTTATTTTTGTCAGATa                       <  2:1904838/150‑1 (MQ=255)
                                                                                                                                 tAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGAAAAAACATATTGGCTGGCTGTTATTTTTGTCAGATAAAc                    >  2:1832251/1‑150 (MQ=255)
                                                                                                                                  aGATGATGTCTTATCTTTTACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGAAAAAACATATTGGCTGGCTGTTATTTTTGTCAGATAAACt                   <  1:2746158/150‑1 (MQ=255)
                                                                                                                                  aGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGAAAAAACATANTGGCTGGCTGTTATTTTTGTCAGATAAACt                   <  1:4278886/150‑1 (MQ=255)
                                                                                                                                     tgatgTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGAAAAAACATATTGGCTGGCTGTTATTTTTGTCAGATAAACtata                <  2:1478991/150‑1 (MQ=255)
                                                                                                                                         gTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGAAAAAACATATTGGCTGGCTGTTATTTTTGTCAGATAAACTAtaattt            <  2:3026232/150‑1 (MQ=255)
                                                                                                                                          tCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGAAAAAACATATTGGCTGGCTGTTATTTTTGTCAGATAAACTAtaatttc           >  1:569535/1‑150 (MQ=255)
                                                                                                                                           cTTATCTTTTACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGAAAAAACATATTGGCTGGCTGTTATTTTTGTCAGATAAACTAtaatttct          >  2:4387095/1‑150 (MQ=255)
                                                                                                                                            ttATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGACCTTTATCACGACCAAGGAAAAAACATATTGGCTGGCTGTTATTTTTGTCAGATAAACTAtaatttcta         <  2:3098951/150‑1 (MQ=255)
                                                                                                                                              aTCTTTTACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGAAAAAACATATTGGCTGGCTGTTATTTTTGTCAGATAAACTAtaatttctaat       <  1:3635657/150‑1 (MQ=255)
                                                                                                                                              aTCTTTTACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGAAAAAACATATTGGCTGGCTGTTATTTTTGTCAGATAAACTAtaatttctaat       <  1:3635301/150‑1 (MQ=255)
                                                                                                                                              aTCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGAAAAAACATATTGGCTGGCTGTTATTTTTGTCAGATAAACTAtaatttctaat       >  2:77590/1‑150 (MQ=255)
                                                                                                                                               tCTTTTACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGAAAAAACATATTGGCTGGCTGTTATTTTTGTCAGATAAACTAtaatttctaatt      <  2:4017876/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CCTCAAGTATACGAAGAGTCCAGCCAGATTGGTTTTCTTTGGTTATATCAACAATTATATTAGCATTTAATGTTCTTCTAATATTCTCTTCATAAGATATTTGTTTTTCAATCAAATACTTTGAAGTCGTAGATGATGTCTTATCTTTGACAACATTAAAATCTAGCTTACATCCTAGTGTTGTTAATCTCTCGGCTAACTCATTAATTATTTGAAGACGCCCTTTATCACGACCAAGGAAAAAACATATTGGCTGGCTGTTATTTTTGTCAGATAAACTATAATTTCTAATTTCAT  >  CP009273/3797759‑3798055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A