Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F54 I0 R1 640 319.5 10493376 95.3% 10000187 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 971,532 A→T 12.4% Q152L (CAG→CTG)  mukE → chromosome condensin MukBEF, MukE localization factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273971,5320AT12.4% 1031.7 / 116.2 330Q152L (CAG→CTG) mukEchromosome condensin MukBEF, MukE localization factor
Reads supporting (aligned to +/- strand):  ref base A (127/162);  new base T (17/24);  total (144/186)
Fisher's exact test for biased strand distribution p-value = 8.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.02e-01

Only 100 of 331 total aligned reads displayed.

CTATCTCAGCCCGGAACGGCTGGCGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAG  >  CP009273/971383‑971680
                                                                                                                                                     |                                                                                                                                                    
ctatctcAGCCCGGAACGGCTGGCGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCa                                                                                                                                                      >  2:3850835/1‑150 (MQ=255)
  atctcAGCCCGGAACGGCTGGCGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAgg                                                                                                                                                    >  1:4604399/1‑150 (MQ=255)
    ctcAGCCCGGAACGGCTGGCGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGgag                                                                                                                                                  <  1:2253354/150‑1 (MQ=255)
    ctcAGCCCGGAACGGCTGGCGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGgag                                                                                                                                                  >  1:39207/1‑150 (MQ=255)
     tcAGCCCGGACCGGCTGGCGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAACCTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGACAGAAGTTGCAGgaga                                                                                                                                                 <  2:4410050/150‑1 (MQ=255)
     tcAGCCCGGAACGGCTGGCGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGgaga                                                                                                                                                 >  1:2980582/1‑150 (MQ=255)
     tcAGCCCGGAACGGCTGGCGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGgaga                                                                                                                                                 >  1:2656415/1‑150 (MQ=255)
     tcAGCCCGGAACGGCTGGCGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGgaga                                                                                                                                                 <  1:4550807/150‑1 (MQ=255)
      cAGCCCGGAACGGCTGGCGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGaa                                                                                                                                                >  2:3995588/1‑150 (MQ=255)
       aGCCCGGAACGGCTGGCGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGaaa                                                                                                                                               >  2:1306252/1‑150 (MQ=255)
        gCCCGGAACGGCTGGCGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAg                                                                                                                                              <  2:3312796/150‑1 (MQ=255)
         cccGGTACGGCTGGCGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCAGTAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGt                                                                                                                                             <  2:2727221/150‑1 (MQ=255)
         cccGGAACGGCTGGCGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCTGGAGAAAGt                                                                                                                                             <  1:2377244/150‑1 (MQ=255)
         cccGGAACGGCTGGCGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGt                                                                                                                                             <  2:2681192/150‑1 (MQ=255)
          ccGGAACGGCTGGCGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTa                                                                                                                                            >  1:3192681/1‑150 (MQ=255)
           cGGAACGGCTGGCGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTAc                                                                                                                                           >  1:1045018/1‑150 (MQ=255)
           cGGAACGGCTGGCGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTAc                                                                                                                                           <  1:3564672/150‑1 (MQ=255)
            ggAACGGCTGGCGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACg                                                                                                                                          >  1:519638/1‑150 (MQ=255)
            ggAACGGCTGGCGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACg                                                                                                                                          <  1:4881010/150‑1 (MQ=255)
            ggAACGGCTGGCGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACg                                                                                                                                          >  2:1377650/1‑150 (MQ=255)
                cGGCTGGCGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCTGGAGAAAGTACGttct                                                                                                                                      <  2:4132073/150‑1 (MQ=255)
                 ggCTGGCGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGttctt                                                                                                                                     <  2:3244511/150‑1 (MQ=255)
                       cGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCa                                                                                                                               >  2:2689954/1‑150 (MQ=255)
                        gAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCTACCGGTTCAGACGTTGACCGTCAGAAGGTGCAGGAGAAAGTCCGTTCTTCGCTCaa                                                                                                                              >  2:2810895/1‑150 (MQ=255)
                          aTGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAAcc                                                                                                                            <  2:2565218/150‑1 (MQ=255)
                          aTGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAAcc                                                                                                                            <  2:5192295/150‑1 (MQ=255)
                          aTGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAAcc                                                                                                                            <  1:5066526/150‑1 (MQ=255)
                            gAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGt                                                                                                                          >  2:3609254/1‑150 (MQ=255)
                             aGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTc                                                                                                                         >  1:1618576/1‑150 (MQ=255)
                             aGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTc                                                                                                                         >  2:2105081/1‑150 (MQ=255)
                              ggggATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCt                                                                                                                        <  1:840458/150‑1 (MQ=255)
                                ggATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGc                                                                                                                      >  2:2502638/1‑150 (MQ=255)
                                ggATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGc                                                                                                                      >  1:398857/1‑150 (MQ=255)
                                 gATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCTGGAGAAAGTACGTTCTTCGCTCAACCGTCTGcg                                                                                                                     <  1:3325157/150‑1 (MQ=255)
                                   ttttCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGcgtc                                                                                                                   <  2:1097683/150‑1 (MQ=255)
                                   ttttCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGcgtc                                                                                                                   <  2:4852732/150‑1 (MQ=255)
                                     ttCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGcgtcgt                                                                                                                 <  1:424889/150‑1 (MQ=255)
                                      tCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGtt                                                                                                                >  2:3165657/1‑150 (MQ=255)
                                      tCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGtt                                                                                                                <  1:974000/150‑1 (MQ=255)
                                        aCCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGCTGCAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTa                                                                                                              <  2:2769242/150‑1 (MQ=255)
                                           cagcagGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGc                                                                                                           >  1:3817261/1‑150 (MQ=255)
                                            agcagGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCa                                                                                                          >  1:3743336/1‑150 (MQ=255)
                                            agcagGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCa                                                                                                          <  1:4990372/150‑1 (MQ=255)
                                               agGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATgg                                                                                                       <  1:1088702/150‑1 (MQ=255)
                                                    cTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTgg                                                                                                  <  2:3013848/150‑1 (MQ=255)
                                                       tACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGttt                                                                                               >  1:51215/1‑150 (MQ=255)
                                                       tACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGttt                                                                                               >  1:209645/1‑150 (MQ=255)
                                                         cgacgaACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTAt                                                                                             <  2:1622201/150‑1 (MQ=255)
                                                         cgacgaACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTAt                                                                                             >  1:1415811/1‑150 (MQ=255)
                                                                 tGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACg                                                                                     >  1:1575851/1‑150 (MQ=255)
                                                                   cTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCTGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGAc                                                                                   >  1:1222902/1‑150 (MQ=255)
                                                                    tCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCTGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGAca                                                                                  <  2:3472787/150‑1 (MQ=255)
                                                                     cACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGAcag                                                                                 <  2:4139211/150‑1 (MQ=255)
                                                                       cccTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGAcagca                                                                               <  1:994482/150‑1 (MQ=255)
                                                                       cccTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGAcagca                                                                               <  1:3204554/150‑1 (MQ=255)
                                                                       cccTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGAcagca                                                                               <  1:4707760/150‑1 (MQ=255)
                                                                         cTGGCCGATGAATCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCTGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACagcagc                                                                             <  1:1816803/150‑1 (MQ=255)
                                                                           ggCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCaa                                                                           <  1:3489866/150‑1 (MQ=255)
                                                                              cGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGtt                                                                        <  2:4787309/150‑1 (MQ=255)
                                                                               gATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGttt                                                                       <  1:1418939/150‑1 (MQ=255)
                                                                                aTGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCTGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTc                                                                      <  2:3133630/150‑1 (MQ=255)
                                                                                aTGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCTGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTc                                                                      >  1:2701938/1‑150 (MQ=255)
                                                                                aTGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCTGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTc                                                                      >  2:2518491/1‑150 (MQ=255)
                                                                                   aaGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCa                                                                   >  2:4508178/1‑150 (MQ=255)
                                                                                     gCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCAtt                                                                 <  1:2203115/150‑1 (MQ=255)
                                                                                         aaCTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCg                                                             >  2:1884960/1‑150 (MQ=255)
                                                                                         aaCTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGGTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCg                                                             >  2:2307629/1‑150 (MQ=255)
                                                                                          aCTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGa                                                            <  1:4441358/150‑1 (MQ=255)
                                                                                            tgctgAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAAt                                                          >  1:2510848/1‑150 (MQ=255)
                                                                                               tgAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCgg                                                       <  2:1345132/150‑1 (MQ=255)
                                                                                                 aaaCTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCTGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGgtg                                                     >  2:2660561/1‑150 (MQ=255)
                                                                                                     tGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTcc                                                 <  2:4555010/150‑1 (MQ=255)
                                                                                                     tGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTcc                                                 >  2:2954325/1‑150 (MQ=255)
                                                                                                       gTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGc                                               >  1:640821/1‑150 (MQ=255)
                                                                                                         gAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCCACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCtt                                             <  1:1837596/150‑1 (MQ=255)
                                                                                                         gAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCtt                                             >  2:3569357/1‑150 (MQ=255)
                                                                                                          aacaacCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTc                                            <  1:110915/150‑1 (MQ=255)
                                                                                                          aacaacCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTc                                            >  2:796855/1‑150 (MQ=255)
                                                                                                               ccGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGc                                       >  1:4470093/1‑150 (MQ=255)
                                                                                                                cGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGcc                                      <  2:875311/150‑1 (MQ=255)
                                                                                                                   tCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCTGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAt                                   >  1:540881/1‑150 (MQ=255)
                                                                                                                     aaCCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAtgt                                 <  2:5122342/150‑1 (MQ=255)
                                                                                                                     aaCCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAtgt                                 >  2:4582578/1‑150 (MQ=255)
                                                                                                                     aaCCGGTTCAGACGTTGACCGTAATAAGATGCATGAGAAAGTACGTGCTTCTCTCAACCGACTGAGTCGTTTAGGCATGGAGTGGTTTAAGGGCCACGAAAGCAGAAAGTTTCGCATTACCGAATCGGAGTTCCGATTCTGAGCCGAtgt                                 >  1:4781837/1‑150 (MQ=255)
                                                                                                                       ccGGTTCAGACGTTGACCGTCAGAAGTTGCTGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCATGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATgtgc                               <  1:179849/150‑1 (MQ=255)
                                                                                                                       ccGGTTCAGACGTTGACCGTCAGAAGGTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATgtgc                               <  2:1486142/150‑1 (MQ=255)
                                                                                                                        cGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGGCAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATgtgcg                              <  2:4205139/150‑1 (MQ=255)
                                                                                                                        cGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATgtgcg                              >  1:1473501/1‑150 (MQ=255)
                                                                                                                          gTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATgtgcgtg                            >  1:2814145/1‑150 (MQ=255)
                                                                                                                             cAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTg                         <  2:1451472/150‑1 (MQ=255)
                                                                                                                              aGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTgg                        <  2:4604399/150‑1 (MQ=255)
                                                                                                                              aGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTgg                        >  1:2019033/1‑150 (MQ=255)
                                                                                                                                 cGTTGACCGTCAGAAGTTGCTGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGcga                     <  2:4969796/150‑1 (MQ=255)
                                                                                                                                 cGTTGACCGTCAGAAGTTGCTGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGcga                     >  2:4469438/1‑150 (MQ=255)
                                                                                                                                   ttGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGcgacg                   <  1:1156954/150‑1 (MQ=255)
                                                                                                                                    tGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGcgacga                  >  2:636607/1‑150 (MQ=255)
                                                                                                                                      aCCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATc                >  2:2453529/1‑150 (MQ=255)
                                                                                                                                              aaGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGaa        >  1:2764156/1‑150 (MQ=255)
                                                                                                                                                   gCAGGAGAAAGTACGTTCGTCGCTCACCCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGcaca   <  1:4662554/150‑1 (MQ=255)
                                                                                                                                                    cAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAg  >  2:4317032/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CTATCTCAGCCCGGAACGGCTGGCGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAG  >  CP009273/971383‑971680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A