Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F54 I0 R1 640 319.5 10493376 95.3% 10000187 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,192,525 C→T 13.5% S155N (AGC→AAC)  ymfD ← e14 prophage; putative SAM‑dependent methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,192,5250CT13.5% 894.0 / 101.6 304S155N (AGC→AAC) ymfDe14 prophage; putative SAM‑dependent methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (149/114);  new base T (21/20);  total (170/134)
Fisher's exact test for biased strand distribution p-value = 6.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 304 total aligned reads displayed.

ACTTTAATATTTCAAGGCCATGTGAAGAACATATTTCTTGCACTGCGAGTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGAGAGAACATTAGAGCAAAGGATGAAATCGTAACCCCCAATTATTTTGTC  >  CP009273/1192379‑1192673
                                                                                                                                                  |                                                                                                                                                    
aCTTTAATATTTCAAGGCCATGTGAAGAACATATTTCTTGCACTGCGAGTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTA‑TTTTTGAAGTATGAGCtttt                                                                                                                                                  >  2:1384261/1‑150 (MQ=255)
 cTTTAATATTTCAAGGCCATGTGAAGAACATATTTCTTGCACTGCGAGTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCtttt                                                                                                                                                  <  1:3265285/150‑1 (MQ=255)
     aaTATTTCAAGGCCATGTGAAGAACATATTTCTTGCACTGCGAGTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTatat                                                                                                                                              >  1:4726341/1‑150 (MQ=255)
      atatTTCAAGGCCATGTGAAGAACATATTTCTTGCACTGCGAGTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATAtt                                                                                                                                             >  1:1703872/1‑150 (MQ=255)
      atatGTCATGTCCATGTGAAGATCTTATTTCTTGCACTTCGAGTTCATCGATTAAACCATAGTAAGAAACTCTTTTTGTATTTTTGTAAATGTATCCGTAAAGTTGTTTTCTTCCTGTTTCGTTTTTTTTGAAGTATGAGTTTTTATAtt                                                                                                                                             <  2:3201254/150‑1 (MQ=255)
       tatTTCAAGGCCATGTGAAGAACATATTTCTTGCACTGCGAGTTCATCGAGTAATCAATAGTAAGAAACACTTATTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTACTGTTTCGTATTTTTTGAAGAATGAGCTTTTATATTg                                                                                                                                            >  2:1792942/1‑150 (MQ=255)
         tttCAAGGCCATGTGAAGAACATATTTCTTGCACTGCGAGTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGAt                                                                                                                                          <  1:4587263/150‑1 (MQ=255)
          ttCAAGGCCATGTGAAGAACATATTTCTTGCACTGCGAGTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGAtt                                                                                                                                         >  1:1559463/1‑150 (MQ=255)
           tCAAGGCCATGTGAAGAACATATTTCTTGCACTGCGAGTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGAttt                                                                                                                                        <  2:2109721/150‑1 (MQ=255)
           tCAAGGCCATGTGAAGAACATATTTCTTGCACTGCGAGTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGAttt                                                                                                                                        <  2:716817/150‑1 (MQ=255)
             aaGGCCATGTGAAGAACATATTTCTTGCACTGCGAGTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTAc                                                                                                                                      >  2:2229944/1‑150 (MQ=255)
                gCCATGTGAAGAACATATTTCTTGCACTGCGAGTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAAt                                                                                                                                   <  2:4966107/150‑1 (MQ=255)
                gCCATGTGAAGAACATATTTCTTGCACTGCGAGTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAAt                                                                                                                                   <  1:1659543/150‑1 (MQ=255)
                 ccATGTGAAGAACATATTTCTTGCACTGCGAGTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATa                                                                                                                                  <  1:3931870/150‑1 (MQ=255)
                   aTGTGAAGAACATATTTCTTGCACTGCGAGTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAag                                                                                                                                >  1:2061480/1‑150 (MQ=255)
                     gtgAAGAACATATTTCTTGCACTGCGAGTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTTATTTACAATAagag                                                                                                                              >  2:3270346/1‑150 (MQ=255)
                      tgAAGAACATATTTCTTGCACTGCGAGTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGt                                                                                                                             <  2:4594195/150‑1 (MQ=255)
                       gaagaaCATATTTCTTGCACTGCGAGTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGtc                                                                                                                            <  1:3868505/150‑1 (MQ=255)
                         agaaCATATTTCTTGCACTGCGAGTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGACTTTGTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGtctc                                                                                                                          <  2:1613677/150‑1 (MQ=255)
                           aaCATTTTTCTTGCACTGCGAGTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGTTTTTATATTGATTTACAATAAGAGTCTCAc                                                                                                                        <  1:3134875/150‑1 (MQ=255)
                             cATATTTCTTGCACTGCGAGTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCAcct                                                                                                                      >  1:4514808/1‑150 (MQ=255)
                              atatTTCTTGCACTGCGAGTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCAcctc                                                                                                                     >  2:4836600/1‑150 (MQ=255)
                              atatTTCTTGCACTGCGAGTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTGTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCAcctc                                                                                                                     <  1:3656959/150‑1 (MQ=255)
                                       gCACTGCGAGTTAATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGTTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTa                                                                                                            <  2:2727073/150‑1 (MQ=255)
                                        cACTGCGAGTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAg                                                                                                           >  2:4557595/1‑150 (MQ=255)
                                         aCTGCGAGTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGt                                                                                                          >  2:3562134/1‑150 (MQ=255)
                                          cTGCGAGTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGTTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTa                                                                                                         <  2:2522456/150‑1 (MQ=255)
                                             cGAGTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAAtc                                                                                                      <  2:4521207/150‑1 (MQ=255)
                                             cGAGTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAAtc                                                                                                      <  1:2619247/150‑1 (MQ=255)
                                                gTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATctct                                                                                                   >  2:4732530/1‑150 (MQ=255)
                                                   cATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGa                                                                                                >  1:3619776/1‑150 (MQ=255)
                                                    aTCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGAt                                                                                               <  2:404559/150‑1 (MQ=255)
                                                       gattAATCCATACTAAGAAACACTTTTTGAATTTTAGTAAATGTATCCGTTTAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTTTGAGCTTTTATATTGATTTACTATAAGTGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCt                                                                                            <  1:4446043/147‑1 (MQ=255)
                                                       gAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCt                                                                                            >  2:1466807/1‑150 (MQ=255)
                                                        aGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTa                                                                                           <  1:1930465/150‑1 (MQ=255)
                                                           aaTCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGa                                                                                        >  1:966053/1‑150 (MQ=255)
                                                           aaTCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGa                                                                                        >  2:1514925/1‑150 (MQ=255)
                                                              ccATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGTTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACt                                                                                     >  2:3055254/1‑150 (MQ=255)
                                                                  aGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTAttt                                                                                 <  2:580372/150‑1 (MQ=255)
                                                                  aGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTAttt                                                                                 >  1:2965714/1‑150 (MQ=255)
                                                                    tAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTg                                                                               <  1:2476683/150‑1 (MQ=255)
                                                                    tAAGAAACACTTGTTGAATTTTTGTAAATGTTTCCGTAAAGTTGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACTATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTg                                                                               <  2:4222587/150‑1 (MQ=255)
                                                                      aGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATATTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTc                                                                             <  1:1222451/150‑1 (MQ=255)
                                                                      aGAAACACTTTTTGAATTTTTGTAAATGTATCCGNAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTc                                                                             >  1:4294343/1‑150 (MQ=255)
                                                                       gAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCg                                                                            <  2:3487357/150‑1 (MQ=255)
                                                                         aaCACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAATAGTCTCACCTCCTGATTTTAGTAATCTCTTTATGCTAAGAACTATTTTTTCGAt                                                                          >  1:142447/1‑150 (MQ=255)
                                                                         aaCACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGAt                                                                          <  2:5042053/150‑1 (MQ=255)
                                                                         aaCACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGAt                                                                          >  1:5114248/1‑150 (MQ=255)
                                                                           cacTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTg                                                                        <  1:1725629/150‑1 (MQ=255)
                                                                             cTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTa                                                                      >  1:4367817/1‑150 (MQ=255)
                                                                                    aaTTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGAc                                                               >  1:3627002/1‑150 (MQ=255)
                                                                                     aTTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACa                                                              >  1:2151265/1‑150 (MQ=255)
                                                                                     aTTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACa                                                              <  2:2245931/150‑1 (MQ=255)
                                                                                          tGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGaa                                                         <  1:2423728/150‑1 (MQ=255)
                                                                                            tAACTGTTTCCGTAAAGATGTTTTCTTCCTGTTTCGTAATTTTTGAATTATGAGTTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACg                                                       <  2:3628127/150‑1 (MQ=255)
                                                                                              aaTGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGc                                                     >  1:3321557/1‑150 (MQ=255)
                                                                                              aaTGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGc                                                     >  1:694986/1‑150 (MQ=255)
                                                                                                 gTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCaga                                                  >  2:568539/1‑150 (MQ=255)
                                                                                                  tATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCagag                                                 <  2:1135793/150‑1 (MQ=255)
                                                                                                  tATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCagag                                                 >  2:1641785/1‑150 (MQ=255)
                                                                                                       gTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGTTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGAGAGAAc                                            >  1:4409260/1‑150 (MQ=255)
                                                                                                       gTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGTTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGAGAGAAc                                            <  1:490917/150‑1 (MQ=255)
                                                                                                       gTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGAGAGAAc                                            <  2:3342303/150‑1 (MQ=255)
                                                                                                       gTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGAGAGAAc                                            >  1:1028205/1‑150 (MQ=255)
                                                                                                       gTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGAGAGAAc                                            <  2:3758079/150‑1 (MQ=255)
                                                                                                       gTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGAGAGAAc                                            <  1:2856487/150‑1 (MQ=255)
                                                                                                       gTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGAGAGAAc                                            >  1:4743400/1‑150 (MQ=255)
                                                                                                       gTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGAGAGAAc                                            <  1:3194279/150‑1 (MQ=255)
                                                                                                        tAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGTTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGAGAGAACa                                           >  2:1396812/1‑150 (MQ=255)
                                                                                                           aGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGAGAGAACATTa                                        >  1:501245/1‑150 (MQ=255)
                                                                                                            gATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGTTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGAGAGAACATTag                                       >  2:2654605/1‑150 (MQ=255)
                                                                                                              tGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGATAGAACATTagag                                     >  2:1208845/1‑150 (MQ=255)
                                                                                                               gTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGAGAGAACATTAGAGc                                    >  1:3769472/1‑150 (MQ=255)
                                                                                                                ttttCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGAGAGAACATTAGAGCa                                   <  1:2210276/150‑1 (MQ=255)
                                                                                                                   tcttcCTGTTTCGTATTTTTTGAAGTATGAGTTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGAGAGAACATTAGAGCAAAg                                <  2:1151699/150‑1 (MQ=255)
                                                                                                                    cttcCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGAGAGAACATTAGAGCAAAgg                               >  2:3923698/1‑150 (MQ=255)
                                                                                                                    cttcCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGAGAGAACATTAGAGCAAAgg                               >  2:5207884/1‑150 (MQ=255)
                                                                                                                        cTGTTTCGTATTTTTTGAAGTATGATCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTTATTTTATTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACTGCAGAGAGTACATTAGAGCAAAGGATGa                           <  2:2976934/150‑1 (MQ=255)
                                                                                                                            ttCGTATTTTTTGAAGTATGAGTTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGAGAGAACATTAGAGCAAAGGATGAAATc                       <  1:4330252/150‑1 (MQ=255)
                                                                                                                            ttCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAATTTAATAACATCCTGATTTTAGTAATATATTTATGCTATGAAATATTTTGACGATTTTATCCCGACAGGGAACGCCAGTGTAAACATTGTAGCAAAGTATGaaaac                       >  2:1306742/1‑148 (MQ=255)
                                                                                                                             tCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGAGAGAACATTAGAGCAAAGGATGAAATCg                      >  2:4105964/1‑150 (MQ=255)
                                                                                                                             tCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGAGAGAACATTAGAGCAAAGGATGAAATCg                      >  2:88198/1‑150 (MQ=255)
                                                                                                                              cGTATTTTTTGAAGTATGAGTTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGAGAGAACATTAGAGCAAAGGATGAAATCGt                     >  1:3670301/1‑150 (MQ=255)
                                                                                                                               gTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGAGAGAACATTAGAGCAAAGGATGAAATCGTa                    >  1:4875509/1‑150 (MQ=255)
                                                                                                                                tATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGAGAGAACATTAGAGCAAAGGATGAAATCGTaa                   >  1:2271086/1‑150 (MQ=255)
                                                                                                                                tATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGAGAGAACATTAGAGCAAAGGATGAAATCGTaa                   >  1:462235/1‑150 (MQ=255)
                                                                                                                                 aTTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGAGAGAACATTAGAGCACAGGATGAAATCGTTAc                  >  2:4989203/1‑150 (MQ=255)
                                                                                                                                 aTTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATATTGTCGATTGTATCCCGACAAGGAACGGCTGAGAGAACATTAGAGCAAAGGATGAAATCGTAAc                  >  2:4902123/1‑150 (MQ=255)
                                                                                                                                     tttGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGAGAGAACATTAGAGCAAAGGATGAAATCGTAAccccc              >  2:2395368/1‑150 (MQ=255)
                                                                                                                                     tttGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGAGAGAACATTAGAGCAAAGGATGAAATCGTAAccccc              >  2:2073982/1‑150 (MQ=255)
                                                                                                                                         aaGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGAGAGAACATTAGAGCAAAGGATGAAATCGTAACCCCCAatt          <  2:3065956/150‑1 (MQ=255)
                                                                                                                                          aGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGAGAGAACATTAGAGCAAAGGATGAAATCGTAACCCCCAatta         <  1:2711324/150‑1 (MQ=255)
                                                                                                                                           gTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGAGAGAACATTAGAGCAAAGGATGAAATCGTAACCCCCAattat        <  1:654806/150‑1 (MQ=255)
                                                                                                                                           gTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGAGAGAACATTAGAGCAAAGGATGAAATCGTAACCCCCAattat        >  1:1856040/1‑150 (MQ=255)
                                                                                                                                            tATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGAGAGAACATTAGAGCAAAGGATGAAATCGTAACCCCCAattatt       >  1:796968/1‑150 (MQ=255)
                                                                                                                                            tATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGAGAGAACATTAGAGCAAAGGATGAAATCGTAACCCCCAattatt       >  1:3421342/1‑150 (MQ=255)
                                                                                                                                             aTGAGTTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGAGAGAACATTAGAGCAAAGGATGAAATCGTAACCCCCAATTAttt      <  2:4236176/150‑1 (MQ=255)
                                                                                                                                             aTGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTT‑GTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGAGAGAACATTAGAGCAAAGGATGAAATCGTAACCCCCAATTAtttt     >  2:1954590/1‑150 (MQ=255)
                                                                                                                                              tGAGTTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGAGAGAACATTAGAGCAAAGGATGAAATCGTAACCCCCAATTAtttt     >  1:1133140/1‑150 (MQ=255)
                                                                                                                                                 gCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGAGAGAACATTAGAGCAAAGGATGAAATCGTAACCCCCAATTATTTTGTc  <  1:4149190/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
ACTTTAATATTTCAAGGCCATGTGAAGAACATATTTCTTGCACTGCGAGTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAATGTATCCGTAAAGATGTTTTCTTCCTGTTTCGTATTTTTTGAAGTATGAGCTTTTATATTGATTTACAATAAGAGTCTCACCTCCTGATTTTAGTAATCTCTTGATGCTAAGAACTATTTTGTCGATTGTATCCCGACAAGGAACGGCAGAGAGAACATTAGAGCAAAGGATGAAATCGTAACCCCCAATTATTTTGTC  >  CP009273/1192379‑1192673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A