Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F86 I0 R1 593 297.4 9515568 94.4% 8982696 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 132,632 A→T 12.4% V107E (GTG→GAG)  speE ← spermidine synthase (putrescine aminopropyltransferase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273132,6320AT12.4% 758.1 / 67.7 239V107E (GTG→GAG) speEspermidine synthase (putrescine aminopropyltransferase)
Reads supporting (aligned to +/- strand):  ref base A (103/105);  new base T (17/13);  total (121/118)
Fisher's exact test for biased strand distribution p-value = 5.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 251 total aligned reads displayed.

AGATAATGACATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACTCGTCGCG  >  CP009273/132483‑132780
                                                                                                                                                      |                                                                                                                                                    
 gaTAATGACATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCCCATCGATTTacaa                                                                                                                                                     >  1:1579162/1‑149 (MQ=255)
  aTAATGACATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTccacc                                                                                                                                                    >  2:190770/1‑150 (MQ=255)
     aTGACATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATc                                                                                                                                                 <  1:3045478/150‑1 (MQ=255)
      tGACATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCg                                                                                                                                                >  1:2298648/1‑150 (MQ=255)
      tGACATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTGAT‑TGTTGGGTAGATAATTACGGCAGAACGATAAGACACCCGCAACGATTTacaacaaag                                                                                                                                                >  2:3608360/1‑147 (MQ=255)
       gACATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATAGt                                                                                                                                               >  2:3162323/1‑150 (MQ=255)
       gACATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATAATGACGGCAGAAAGATACGACACCCGCATCGATTTACACCATCGt                                                                                                                                               >  2:1976718/1‑150 (MQ=255)
        aCATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTGAT‑GGTTGGGTAGATACAGACGGCAGAACGATACGACACCCCCAACGATTTCCACCAACGTg                                                                                                                                              >  1:941511/1‑150 (MQ=255)
         cATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGa                                                                                                                                             >  2:3529036/1‑150 (MQ=255)
         cATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGa                                                                                                                                             >  2:371671/1‑150 (MQ=255)
          aTCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTATGGGTTGGGT‑GATACATAAGGCAGAACCAAACCACACCCCCAACGATTTCCACCATAGTGAt                                                                                                                                            >  1:4362776/1‑150 (MQ=255)
            cAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTACTCCATCGTGATTg                                                                                                                                          >  1:667683/1‑150 (MQ=255)
             aaaGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACATAAGGCACAACGATACGACACCCGCATCGATTTCCACCAACGTGATTGa                                                                                                                                         >  2:438516/1‑150 (MQ=255)
              aaGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTCCCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCTCCATCGTGATTGAc                                                                                                                                        >  2:3909468/1‑150 (MQ=255)
               aGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGTCGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACt                                                                                                                                       >  2:2961042/1‑150 (MQ=255)
                ggTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTc                                                                                                                                      <  1:103603/150‑1 (MQ=255)
                ggTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTc                                                                                                                                      >  2:2185866/1‑150 (MQ=255)
                 gTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCa                                                                                                                                     >  2:3384767/1‑150 (MQ=255)
                    tggctggTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACg                                                                                                                                  <  1:2377379/150‑1 (MQ=255)
                    tggctggTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACg                                                                                                                                  <  1:2643879/150‑1 (MQ=255)
                      gctggTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGtt                                                                                                                                <  1:2540868/150‑1 (MQ=255)
                      gctggTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGtt                                                                                                                                <  2:708129/150‑1 (MQ=255)
                        tggTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGtttt                                                                                                                              <  2:2517212/150‑1 (MQ=255)
                        tggTTTGATTAACGAAATTGACGCCATCGACGATCACCAGATTAAAGCGCGGATCGTCGTAGCTACCGGCTTTAT‑TTTTTGGTAGATACTGACGGCATAACGATAAGAAACACGCAAAGAATTCCACCATCGTGATTGACTCCACGttgt                                                                                                                              >  2:1409353/1‑148 (MQ=255)
                         ggTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCTCCATCGTGATTGACTCAACGttttt                                                                                                                             >  2:4144249/1‑150 (MQ=255)
                         ggTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCTCCATCGTGATTGACTCAACGttttt                                                                                                                             >  2:247229/1‑150 (MQ=255)
                           tttGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAAAGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTAt                                                                                                                           >  1:215073/1‑150 (MQ=255)
                            ttGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCTCCATCGTGATTGACTCAACGTTTTTATg                                                                                                                          <  1:52301/150‑1 (MQ=255)
                             tGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCTCCATCGTGATTGACTCAACGTTTTTATGt                                                                                                                         <  1:3732160/150‑1 (MQ=255)
                             tGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGt                                                                                                                         <  1:4222644/150‑1 (MQ=255)
                               aTTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCg                                                                                                                       >  2:2125677/1‑150 (MQ=255)
                                ttAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCTCCATCGTGATTGACTCAACGTTTTTATGTCgg                                                                                                                      >  2:305819/1‑150 (MQ=255)
                                  aaCGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCgggg                                                                                                                    >  1:1855464/1‑149 (MQ=255)
                                     gAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTAGGGTTAc                                                                                                                 >  2:528529/1‑150 (MQ=255)
                                      aaaTTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACt                                                                                                                >  2:4356927/1‑150 (MQ=255)
                                       aaTTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACtt                                                                                                               <  2:2719683/150‑1 (MQ=255)
                                       aaTTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCAACGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTAcgt                                                                                                               >  1:2620891/1‑148 (MQ=255)
                                           gACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACg                                                                                                           <  1:1314642/150‑1 (MQ=255)
                                           gACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACg                                                                                                           <  1:4681226/150‑1 (MQ=255)
                                             cGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACgca                                                                                                         <  2:3434329/150‑1 (MQ=255)
                                                cATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑TGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCCTCGATTTCCACCAACGTGTTTTACTCAACGTTTTTATGTGGGGTTAATTCACgcagca                                                                                                      >  1:1980432/1‑150 (MQ=255)
                                                  tcgtcgATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATg                                                                                                    <  2:2445056/150‑1 (MQ=255)
                                                  tcgtcgATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATg                                                                                                    <  2:1094197/150‑1 (MQ=255)
                                                  tcgtcgATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATg                                                                                                    <  1:3142764/150‑1 (MQ=255)
                                                  tcgtcgATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACATACGGCAGAACGAAAAGACACCCCCATCGATTTCCACCAACCGGATTGACACAACGTTTTTATGTAGGGTTACTTCACGCAGCATg                                                                                                    >  2:1623843/1‑150 (MQ=255)
                                                   cgtcgATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATgg                                                                                                   >  2:1803968/1‑150 (MQ=255)
                                                     tcgATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCa                                                                                                 <  1:612145/150‑1 (MQ=255)
                                                     tcgATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCa                                                                                                 <  1:2959633/150‑1 (MQ=255)
                                                     tcgATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCa                                                                                                 <  1:4748443/150‑1 (MQ=255)
                                                       gATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATTTCGGGTTACTTAACGCAGCATGGCAcc                                                                                               >  1:3038979/1‑150 (MQ=255)
                                                       gATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCAcc                                                                                               >  1:1507959/1‑150 (MQ=255)
                                                         tCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCTCCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGt                                                                                             >  1:3538792/1‑150 (MQ=255)
                                                          cACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTc                                                                                            >  2:3753783/1‑150 (MQ=255)
                                                             cAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTcgcc                                                                                         <  2:1086704/150‑1 (MQ=255)
                                                                 ttAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTcgccgccg                                                                                     <  1:683456/150‑1 (MQ=255)
                                                                 ttAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTcgccgccg                                                                                     <  2:665152/150‑1 (MQ=255)
                                                                 ttAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTcgccgccg                                                                                     <  2:3737747/150‑1 (MQ=255)
                                                                  tAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTcgccgccgc                                                                                    >  1:2724117/1‑150 (MQ=255)
                                                                    aaGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGccgccgccg                                                                                  <  2:405599/150‑1 (MQ=255)
                                                                    aaGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGccgccgccg                                                                                  <  1:3437013/150‑1 (MQ=255)
                                                                     aGCGCGGATCGTCGTAGCTACCGGCGTTAA‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGCTTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACATCACGCCGCATGGCACCGTCcccgcccccca                                                                                 >  1:3852607/1‑148 (MQ=255)
                                                                        gcgGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCTCCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATaa                                                                              <  2:1334833/150‑1 (MQ=255)
                                                                          ggATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATc                                                                            <  1:71032/150‑1 (MQ=255)
                                                                                 cgtAGCTACCGGCGTTAT‑GGTTGGGTAGATACTTAAGGCAGAACGATACCACACCCCCAACGATTTACACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCCTGGCACCGTCGCCGCCCCCGCTAAACAGcccaa                                                                     >  1:3321343/1‑149 (MQ=255)
                                                                                  gtAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGGACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATg                                                                    <  2:1219890/150‑1 (MQ=255)
                                                                                  gtAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATg                                                                    <  1:266185/150‑1 (MQ=255)
                                                                                     gCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCTCCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGttt                                                                 <  2:4030568/150‑1 (MQ=255)
                                                                                      cTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTc                                                                <  1:3210356/150‑1 (MQ=255)
                                                                                         ccGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTcgcg                                                             <  2:2293786/150‑1 (MQ=255)
                                                                                         ccGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTcgcg                                                             <  2:3226878/150‑1 (MQ=255)
                                                                                          cGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGt                                                            <  2:1043764/150‑1 (MQ=255)
                                                                                                  t‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGAGAATCATCACATGTTTCGCGTGACCATgg                                                    >  2:825753/1‑150 (MQ=255)
                                                                                                          gTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTa                                             <  1:4564583/150‑1 (MQ=255)
                                                                                                          gTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTa                                             <  2:1004631/150‑1 (MQ=255)
                                                                                                          gTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTa                                             <  2:184113/150‑1 (MQ=255)
                                                                                                           tAGATACTGACGGCAGAACGAGACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAg                                            <  2:4747877/150‑1 (MQ=255)
                                                                                                            aGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGc                                           >  1:1347438/1‑150 (MQ=255)
                                                                                                              aTACTGACGGCAGAACGATACGACACCCGCATCGATTTCCTCCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCgg                                         >  1:1756432/1‑150 (MQ=255)
                                                                                                              aTACTGACGGCAGAACGATACGACACCCGCATCGATTTCCTCCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCgg                                         <  2:1138850/150‑1 (MQ=255)
                                                                                                               tACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGa                                        >  1:4199554/1‑150 (MQ=255)
                                                                                                                aCTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGaa                                       >  1:3951476/1‑150 (MQ=255)
                                                                                                                    aCGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATg                                   >  1:2148982/1‑150 (MQ=255)
                                                                                                                         aGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATCATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGtca                              >  2:4115439/1‑150 (MQ=255)
                                                                                                                         aGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGtca                              >  2:1366420/1‑150 (MQ=255)
                                                                                                                          gAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGtcat                             >  2:1630982/1‑150 (MQ=255)
                                                                                                                            aCGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGtcatca                           <  2:4229811/150‑1 (MQ=255)
                                                                                                                               aTACGACACCCGCATCGATGTCCACCATCGTGATTGACTCAACGTTTTTCTGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTGTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCAtct                        <  1:556848/150‑1 (MQ=255)
                                                                                                                                tACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCAtctc                       <  1:3467112/150‑1 (MQ=255)
                                                                                                                                 aCGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCa                      <  2:921177/150‑1 (MQ=255)
                                                                                                                                  cGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATTATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCAt                     <  2:1460696/150‑1 (MQ=255)
                                                                                                                                  cGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCAt                     <  2:450197/150‑1 (MQ=255)
                                                                                                                                  cGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCAt                     >  1:4165439/1‑150 (MQ=255)
                                                                                                                                         cGCATCGATTTCCTCCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATgatagat              <  1:3647507/150‑1 (MQ=255)
                                                                                                                                         cGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATgatagat              >  2:768945/1‑150 (MQ=255)
                                                                                                                                           cATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaa            <  1:847324/150‑1 (MQ=255)
                                                                                                                                              cGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACt         <  2:3285237/150‑1 (MQ=255)
                                                                                                                                                aTTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACtcg       <  1:1618760/150‑1 (MQ=255)
                                                                                                                                                   tCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACtcgtcg    >  2:333771/1‑150 (MQ=255)
                                                                                                                                                    ccaccaTCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACTCGTcgc   >  1:2010685/1‑150 (MQ=255)
                                                                                                                                                     caccaTCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACTCGTcgcg  <  2:2602300/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
AGATAATGACATCAAAGGTCTGGCTGGTTTGATTAACGAAATTGACGCCATCGTCGATCACCAGCTTAAAGCGCGGATCGTCGTAGCTACCGGCGTTAT‑GGTTGGGTAGATACTGACGGCAGAACGATACGACACCCGCATCGATTTCCACCATCGTGATTGACTCAACGTTTTTATGTCGGGTTACTTCACGCAGCATGGCACCGTCGCCGCCGCCGATAATCAGCACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAACTCGTCGCG  >  CP009273/132483‑132780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A