Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F86 I0 R1 593 297.4 9515568 94.4% 8982696 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,986,289 T→A 61.1% N4Y (AAT→TAT)  pgsA ← phosphatidylglycerophosphate synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,986,2890TA61.1% 202.4 / 303.0 221N4Y (AAT→TAT) pgsAphosphatidylglycerophosphate synthetase
Reads supporting (aligned to +/- strand):  ref base T (51/35);  new base A (62/73);  total (113/108)
Fisher's exact test for biased strand distribution p-value = 5.51e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 224 total aligned reads displayed.

TGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCTCGACGCTGGCGT  >  CP009273/1986140‑1986437
                                                                                                                                                     |                                                                                                                                                    
tGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATAtt                                                                                                                                                      <  1:2566633/150‑1 (MQ=255)
  ccAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCACACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTaa                                                                                                                                                    >  2:3463952/1‑150 (MQ=255)
     gAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGAGAAAAGACCAATACAAAGAATGGGAGAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAAtt                                                                                                                                                 >  2:4216849/1‑150 (MQ=255)
       aaaCCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGc                                                                                                                                               >  1:3179966/1‑150 (MQ=255)
         aCCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCAt                                                                                                                                             <  1:598633/150‑1 (MQ=255)
          ccATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATa                                                                                                                                            <  1:3436865/150‑1 (MQ=255)
          ccATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATa                                                                                                                                            <  1:353188/150‑1 (MQ=255)
            aTCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAAt                                                                                                                                          <  2:411084/150‑1 (MQ=255)
             tCGAACCAGTCAGTCACCGCCGCTACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATg                                                                                                                                         <  1:1254596/150‑1 (MQ=255)
             tCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATg                                                                                                                                         <  2:459702/150‑1 (MQ=255)
               gAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGAc                                                                                                                                       <  2:4738735/150‑1 (MQ=255)
               gAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGAc                                                                                                                                       <  1:888318/150‑1 (MQ=255)
                  ccAGTCAGTCACGGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACggg                                                                                                                                    >  2:50239/1‑150 (MQ=255)
                   cagtcagtCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGt                                                                                                                                   >  1:3659372/1‑150 (MQ=255)
                    agtcagtcACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTa                                                                                                                                  <  1:149709/150‑1 (MQ=255)
                     gtcagtcaCCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCATGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTaa                                                                                                                                 <  2:3567388/150‑1 (MQ=255)
                     gtcagtcaCCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTaa                                                                                                                                 <  1:933957/150‑1 (MQ=255)
                     gtcagtcaCCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTaa                                                                                                                                 <  2:3567273/150‑1 (MQ=255)
                       cagtcaCCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACt                                                                                                                               <  2:2911456/150‑1 (MQ=255)
                              cgccgcGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCtgtt                                                                                                                        >  1:473521/1‑150 (MQ=255)
                               gccgcGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCtgttg                                                                                                                       <  2:3988617/150‑1 (MQ=255)
                                  gcgACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCa                                                                                                                    <  2:275198/150‑1 (MQ=255)
                                   cgACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAg                                                                                                                   >  2:4032106/1‑150 (MQ=255)
                                     aCGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTa                                                                                                                 <  1:4430475/150‑1 (MQ=255)
                                        cAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGa                                                                                                              <  2:3706518/150‑1 (MQ=255)
                                         aGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGAt                                                                                                             <  1:2752598/150‑1 (MQ=255)
                                         aGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGAt                                                                                                             >  1:3822205/1‑150 (MQ=255)
                                         aGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGAt                                                                                                             <  2:2278499/150‑1 (MQ=255)
                                          gAACATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGAtt                                                                                                            <  2:3175078/150‑1 (MQ=255)
                                          gAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACACAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGAtt                                                                                                            <  2:1075345/150‑1 (MQ=255)
                                              aTGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTAccc                                                                                                        <  1:4618326/150‑1 (MQ=255)
                                                gAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCt                                                                                                      <  1:3964792/150‑1 (MQ=255)
                                                gAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCt                                                                                                      >  2:3227509/1‑150 (MQ=255)
                                                 aGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTa                                                                                                     <  2:4299474/150‑1 (MQ=255)
                                                 aGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTa                                                                                                     <  2:2025040/150‑1 (MQ=255)
                                                   cgcgGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATg                                                                                                   <  2:3262890/150‑1 (MQ=255)
                                                         ggcAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTACGCAACGTAGGGATATTAAATTGCATAATGACGGGTACCTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTa                                                                                             >  2:1743761/1‑150 (MQ=255)
                                                         ggcAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTa                                                                                             >  2:2558719/1‑150 (MQ=255)
                                                         ggcAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTa                                                                                             >  1:764444/1‑150 (MQ=255)
                                                          gcAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTAc                                                                                            <  2:1692711/150‑1 (MQ=255)
                                                           cAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACa                                                                                           >  1:4048658/1‑150 (MQ=255)
                                                            aaaCGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGACTGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACag                                                                                          <  2:3308446/150‑1 (MQ=255)
                                                             aaCGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACaga                                                                                         <  1:2433917/150‑1 (MQ=255)
                                                               cGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAgaga                                                                                       >  2:4672052/1‑150 (MQ=255)
                                                                ggCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGAc                                                                                      <  1:1194209/150‑1 (MQ=255)
                                                                ggCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGAc                                                                                      <  2:1366325/150‑1 (MQ=255)
                                                                  cGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACAt                                                                                    >  2:3817203/1‑150 (MQ=255)
                                                                   gACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATc                                                                                   >  2:1548764/1‑150 (MQ=255)
                                                                     ccAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCaa                                                                                 <  1:1021231/150‑1 (MQ=255)
                                                                       aGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATg                                                                               >  1:3739929/1‑150 (MQ=255)
                                                                       aGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATg                                                                               >  1:743982/1‑150 (MQ=255)
                                                                        gttGACAGGCAGATAAAAGACCATTACAAAGTATGGGATATGGATGACACGGAACAGTGTAAGCAACGTAGGTATATAAAATTGCATAATGACGTGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGt                                                                              <  2:1835838/148‑1 (MQ=255)
                                                                          tGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGttt                                                                            >  2:2331678/1‑150 (MQ=255)
                                                                          tGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGttt                                                                            >  2:1612661/1‑150 (MQ=255)
                                                                           gACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTc                                                                           >  1:4660291/1‑150 (MQ=255)
                                                                            aCAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCa                                                                          <  1:694260/150‑1 (MQ=255)
                                                                              aGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAAc                                                                        <  1:2283958/150‑1 (MQ=255)
                                                                              aGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAAc                                                                        <  1:1431048/150‑1 (MQ=255)
                                                                                   gATAAAAGACCAATCCAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCa                                                                   <  2:608407/150‑1 (MQ=255)
                                                                                   gATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCa                                                                   <  2:2978717/150‑1 (MQ=255)
                                                                                       aaaGACCAATACAAAGAATGGTATAAGGATGACACGGAACAGTGTAAGCAACGTAGGTATATAAAATTGAATAATGACGGGTAACTATCTGTTTTAATTAAGATTACCCCTATGTTGCTACAGAGACATAAATGTTTCAACGACCagaag                                                               <  1:2012290/150‑1 (MQ=255)
                                                                                       aaaGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCagaag                                                               >  1:2312729/1‑150 (MQ=255)
                                                                                        aaGACCAATACAAAGAATGGGATAATGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCagaaga                                                              >  1:2045810/1‑150 (MQ=255)
                                                                                        aaGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCagaaga                                                              >  2:946258/1‑150 (MQ=255)
                                                                                        aaGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCagaaga                                                              >  2:3901183/1‑150 (MQ=255)
                                                                                        aaGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCagaaga                                                              >  1:4551328/1‑150 (MQ=255)
                                                                                          gACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATc                                                            >  1:1817570/1‑150 (MQ=255)
                                                                                          gACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATc                                                            <  2:2128103/150‑1 (MQ=255)
                                                                                          gACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATc                                                            >  1:4201485/1‑150 (MQ=255)
                                                                                           aCCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCt                                                           >  1:4228822/1‑150 (MQ=255)
                                                                                           aCCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCt                                                           >  1:1839560/1‑150 (MQ=255)
                                                                                             cAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCttt                                                         <  2:4167154/150‑1 (MQ=255)
                                                                                              aaTACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCtttt                                                        >  2:2223085/1‑150 (MQ=255)
                                                                                                tACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCt                                                      >  2:3790806/1‑150 (MQ=255)
                                                                                                tACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCt                                                      >  2:1505337/1‑150 (MQ=255)
                                                                                                 aCAAAGAATGGGATAAGGATGACACGGAACTGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTg                                                     <  1:96247/150‑1 (MQ=255)
                                                                                                 aCAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTg                                                     >  2:594443/1‑150 (MQ=255)
                                                                                                  cAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGc                                                    >  2:3883799/1‑150 (MQ=255)
                                                                                                   aaaGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGcc                                                   <  2:3235337/150‑1 (MQ=255)
                                                                                                    aaGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCa                                                  <  2:3057941/150‑1 (MQ=255)
                                                                                                             aTAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCg                                         >  2:1911356/1‑150 (MQ=255)
                                                                                                               aaGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGaa                                       >  2:918768/1‑150 (MQ=255)
                                                                                                                     gACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATAccc                                 >  2:493263/1‑150 (MQ=255)
                                                                                                                       cacGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACAGCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCgg                               <  1:4143614/150‑1 (MQ=255)
                                                                                                                         cGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCa                             <  2:3530450/150‑1 (MQ=255)
                                                                                                                          ggAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCAc                            >  1:2791796/1‑150 (MQ=255)
                                                                                                                          ggAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCAc                            >  1:2408221/1‑150 (MQ=255)
                                                                                                                           gAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACt                           >  1:1645152/1‑150 (MQ=255)
                                                                                                                            aaCAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACtt                          >  2:2716828/1‑150 (MQ=255)
                                                                                                                              cAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACtttt                        <  1:3817203/150‑1 (MQ=255)
                                                                                                                                  gtAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCaa                    >  2:2369160/1‑150 (MQ=255)
                                                                                                                                    aaGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAAtt                  <  1:158834/150‑1 (MQ=255)
                                                                                                                                    aaGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAAtt                  >  1:1577948/1‑150 (MQ=255)
                                                                                                                                    aaGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAAtt                  >  1:2664897/1‑150 (MQ=255)
                                                                                                                                    aaGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAAtt                  >  1:293769/1‑150 (MQ=255)
                                                                                                                                    aaGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAAtt                  >  2:470400/1‑150 (MQ=255)
                                                                                                                                        aaCGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCAAAATACCCGGCACTTTTGCAATTTCCt              >  1:905361/1‑150 (MQ=255)
                                                                                                                                        aaCGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCt              >  1:1312423/1‑150 (MQ=255)
                                                                                                                                               ggATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCTCGACGCt       >  2:109160/1‑150 (MQ=255)
                                                                                                                                               ggATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCTCGACGCt       >  2:2483513/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCTCGACGCTGGCGT  >  CP009273/1986140‑1986437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCTCGA  >  CP009273/1986140‑1986428
                                                                                                                                                     |                                                                                                                                           
TGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATT                                                                                                                                             <  SRR19973082.2582629/150‑1 (MQ=60)
TGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATA                                                                                                                                             <  SRR19973082.2248668/150‑1 (MQ=60)
TGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATA                                                                                                                                             <  SRR19973082.1673503/150‑1 (MQ=60)
 GCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAA                                                                                                                                            <  SRR19973082.2875748/150‑1 (MQ=60)
  CCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAA                                                                                                                                           >  SRR19973082.275300/1‑150 (MQ=60)
       AAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGC                                                                                                                                      >  SRR19973082.3199195/1‑150 (MQ=60)
         ACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCAT                                                                                                                                    <  SRR19973082.1123931/150‑1 (MQ=60)
         ACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCAT                                                                                                                                    <  SRR19973082.603298/150‑1 (MQ=60)
         ACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCAT                                                                                                                                    <  SRR19973082.602887/150‑1 (MQ=60)
         ACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCAT                                                                                                                                    <  SRR19973082.602708/150‑1 (MQ=60)
          CCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATA                                                                                                                                   <  SRR19973082.768952/150‑1 (MQ=60)
          CCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATA                                                                                                                                   <  SRR19973082.768012/150‑1 (MQ=60)
          CCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATA                                                                                                                                   <  SRR19973082.3458033/150‑1 (MQ=60)
          CCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATA                                                                                                                                   <  SRR19973082.908164/150‑1 (MQ=60)
          CCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATA                                                                                                                                   <  SRR19973082.355509/150‑1 (MQ=60)
             TCGAACCAGTCAGTCACCGCCGCTACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATG                                                                                                                                <  SRR19973082.1263206/150‑1 (MQ=60)
              CGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGA                                                                                                                               <  SRR19973082.1215336/150‑1 (MQ=60)
               GAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGAC                                                                                                                              <  SRR19973082.894435/150‑1 (MQ=60)
                   CAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGT                                                                                                                          >  SRR19973082.1641016/1‑150 (MQ=60)
                   CAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGT                                                                                                                          >  SRR19973082.3681964/1‑150 (MQ=60)
                   CAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGT                                                                                                                          >  SRR19973082.3681509/1‑150 (MQ=60)
                    AGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTA                                                                                                                         >  SRR19973082.1841671/1‑150 (MQ=60)
                    AGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTA                                                                                                                         <  SRR19973082.151040/150‑1 (MQ=60)
                    AGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTA                                                                                                                         <  SRR19973082.2542312/150‑1 (MQ=60)
                     GTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAA                                                                                                                        <  SRR19973082.940403/150‑1 (MQ=60)
                     GTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAA                                                                                                                        <  SRR19973082.940421/150‑1 (MQ=60)
                           CACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCT                                                                                                                  >  SRR19973082.2830295/1‑150 (MQ=60)
                            ACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTG                                                                                                                 <  SRR19973082.3687761/150‑1 (MQ=60)
                              CGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTT                                                                                                               >  SRR19973082.476863/1‑150 (MQ=60)
                              CGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTT                                                                                                               <  SRR19973082.2911572/150‑1 (MQ=60)
                               GCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTG                                                                                                              <  SRR19973082.733376/150‑1 (MQ=60)
                                  GCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCA                                                                                                           <  SRR19973082.259514/150‑1 (MQ=60)
                                  GCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCA                                                                                                           <  SRR19973082.3990243/150‑1 (MQ=60)
                                     ACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTA                                                                                                        <  SRR19973082.4457324/150‑1 (MQ=60)
                                         AGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGAT                                                                                                    >  SRR19973082.3845759/1‑150 (MQ=60)
                                         AGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGAT                                                                                                    <  SRR19973082.2769271/150‑1 (MQ=60)
                                         AGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGAT                                                                                                    <  SRR19973082.2769288/150‑1 (MQ=60)
                                         AGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGAT                                                                                                    <  SRR19973082.4325396/150‑1 (MQ=60)
                                         AGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGAT                                                                                                    <  SRR19973082.2452031/150‑1 (MQ=60)
                                          GAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATT                                                                                                   <  SRR19973082.393930/150‑1 (MQ=60)
                                              ATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCC                                                                                               <  SRR19973082.4646340/150‑1 (MQ=60)
                                                GAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCT                                                                                             <  SRR19973082.3988856/150‑1 (MQ=60)
                                                  GCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTAT                                                                                           <  SRR19973082.4044755/150‑1 (MQ=60)
                                                         GGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTA                                                                                    <  SRR19973082.1656893/150‑1 (MQ=60)
                                                         GGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTA                                                                                    >  SRR19973082.769389/1‑150 (MQ=60)
                                                           CAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACA                                                                                  >  SRR19973082.4073479/1‑150 (MQ=60)
                                                           CAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACA                                                                                  >  SRR19973082.4076148/1‑150 (MQ=60)
                                                             AACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGA                                                                                <  SRR19973082.2449054/150‑1 (MQ=60)
                                                             AACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGA                                                                                <  SRR19973082.2448675/150‑1 (MQ=60)
                                                              ACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAG                                                                               <  SRR19973082.2309179/150‑1 (MQ=60)
                                                                GGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGCATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGAC                                                                             <  SRR19973082.1202187/150‑1 (MQ=60)
                                                                     CCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAA                                                                        <  SRR19973082.1028307/150‑1 (MQ=60)
                                                                       AGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATG                                                                      >  SRR19973082.3763059/1‑150 (MQ=60)
                                                                       AGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATG                                                                      >  SRR19973082.748865/1‑150 (MQ=60)
                                                                        GGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGT                                                                     <  SRR19973082.3241983/150‑1 (MQ=60)
                                                                        GGTGACAGGCAGATAAAAGACCAAAACAACGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGT                                                                     <  SRR19973082.855007/150‑1 (MQ=60)
                                                                         GTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTT                                                                    >  SRR19973082.357420/1‑150 (MQ=60)
                                                                         GTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTT                                                                    >  SRR19973082.1937176/1‑150 (MQ=60)
                                                                          TGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTT                                                                   <  SRR19973082.3001505/150‑1 (MQ=60)
                                                                          TGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTT                                                                   <  SRR19973082.2241813/150‑1 (MQ=60)
                                                                          TGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTT                                                                   <  SRR19973082.1443859/150‑1 (MQ=60)
                                                                           GACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTC                                                                  >  SRR19973082.4688445/1‑150 (MQ=60)
                                                                           GACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTC                                                                  >  SRR19973082.4688465/1‑150 (MQ=60)
                                                                            ACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCA                                                                 >  SRR19973082.3843883/1‑150 (MQ=60)
                                                                            ACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCA                                                                 <  SRR19973082.698965/150‑1 (MQ=60)
                                                                            ACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCA                                                                 >  SRR19973082.1437553/1‑150 (MQ=60)
                                                                              AGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAAC                                                               <  SRR19973082.2298220/150‑1 (MQ=60)
                                                                              AGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAAC                                                               <  SRR19973082.1440803/150‑1 (MQ=60)
                                                                                GCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGA                                                             >  SRR19973082.4740520/1‑150 (MQ=60)
                                                                                  AGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACC                                                           >  SRR19973082.959284/1‑150 (MQ=60)
                                                                                       AAAGACCAATACAAAGAATGGTATAAGGATGACACGGAACAGTGTAAGCAACGTAGGTATATAAAATTGAATAATGACGGGTAACTATCTGTTTTAATTAAGATTACCCCTATGTTGCTACAGAGACATAAATGTTTCAACGACCAGAAG                                                      <  SRR19973082.2025008/150‑1 (MQ=60)
                                                                                       AAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAG                                                      >  SRR19973082.2327094/1‑150 (MQ=60)
                                                                                       AAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAG                                                      >  SRR19973082.1443604/1‑150 (MQ=60)
                                                                                        AAGACCAATACAAAGAATGGGATAATGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGA                                                     >  SRR19973082.2058777/1‑150 (MQ=60)
                                                                                        AAGACCAATACAAAGAATGGGATAATGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGA                                                     >  SRR19973082.2058795/1‑150 (MQ=60)
                                                                                        AAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGA                                                     >  SRR19973082.4579025/1‑150 (MQ=60)
                                                                                        AAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGA                                                     >  SRR19973082.4578986/1‑150 (MQ=60)
                                                                                        AAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGA                                                     >  SRR19973082.4578932/1‑150 (MQ=60)
                                                                                          GACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATC                                                   >  SRR19973082.4227131/1‑150 (MQ=60)
                                                                                          GACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATC                                                   >  SRR19973082.1829467/1‑150 (MQ=60)
                                                                                           ACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCT                                                  >  SRR19973082.4254590/1‑150 (MQ=60)
                                                                                           ACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCT                                                  >  SRR19973082.4254492/1‑150 (MQ=60)
                                                                                           ACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCT                                                  >  SRR19973082.1851551/1‑150 (MQ=60)
                                                                                           ACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCT                                                  >  SRR19973082.4214372/1‑150 (MQ=60)
                                                                                                 ACAACGAATGGGATAAGGAGGACACGGAACAGGGTAAGCAACGTAGGGATATATAATTGCATAATGACGGGTACCTATCTGTTGTCAGTAAGATTACCCCTATGGTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCGG                                            <  SRR19973082.97218/150‑1 (MQ=60)
                                                                                                 ACAAAGAATGGGATAAGGATGACACGGAACTGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTG                                            <  SRR19973082.97210/150‑1 (MQ=60)
                                                                                                       AATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGAC                                      >  SRR19973082.3528892/1‑150 (MQ=60)
                                                                                                            GATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGC                                 >  SRR19973082.1787386/1‑150 (MQ=60)
                                                                                                               AAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAA                              >  SRR19973082.953198/1‑150 (MQ=60)
                                                                                                                  GATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATA                           >  SRR19973082.2273327/1‑150 (MQ=60)
                                                                                                                       CACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGG                      <  SRR19973082.151057/150‑1 (MQ=60)
                                                                                                                       CACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACAGCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGG                      <  SRR19973082.4168996/150‑1 (MQ=60)
                                                                                                                          GGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCAC                   >  SRR19973082.2808749/1‑150 (MQ=60)
                                                                                                                          GGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCAC                   >  SRR19973082.2423143/1‑150 (MQ=60)
                                                                                                                          GGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCAC                   >  SRR19973082.2423101/1‑150 (MQ=60)
                                                                                                                           GAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCAAAATACCCGGCACT                  >  SRR19973082.4708289/1‑150 (MQ=60)
                                                                                                                           GAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACT                  >  SRR19973082.1656094/1‑150 (MQ=60)
                                                                                                                              CAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTT               <  SRR19973082.3840739/150‑1 (MQ=60)
                                                                                                                                    AAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATT         >  SRR19973082.1588234/1‑150 (MQ=60)
                                                                                                                                    AAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATT         <  SRR19973082.160232/150‑1 (MQ=60)
                                                                                                                                    AAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATT         >  SRR19973082.1588374/1‑150 (MQ=60)
                                                                                                                                    AAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATT         >  SRR19973082.2681304/1‑150 (MQ=60)
                                                                                                                                    AAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATT         >  SRR19973082.295888/1‑150 (MQ=60)
                                                                                                                                        AACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCAAAATACCCGGCACTTTTGCAATTTCCT     >  SRR19973082.911617/1‑150 (MQ=60)
                                                                                                                                        AACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCT     >  SRR19973082.1320189/1‑150 (MQ=60)
                                                                                                                                        AACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCT     >  SRR19973082.1321498/1‑150 (MQ=60)
                                                                                                                                          CGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCTCG   <  SRR19973082.3837249/150‑1 (MQ=60)
                                                                                                                                           TTAGGTATATAAAATTGCATAATGACGGGTAACTATCTGTTGTAAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATTTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCTCGA  <  SRR19973082.4389633/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                           
TGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCTCGA  >  CP009273/1986140‑1986428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑