Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,986,289 T→A 100% N4Y (AAT→TAT)  pgsA ← phosphatidylglycerophosphate synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,986,2890TA99.5% 603.5 / ‑4.6 181N4Y (AAT→TAT) pgsAphosphatidylglycerophosphate synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base A (100/80);  total (100/81)
Fisher's exact test for biased strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 184 total aligned reads displayed.

AGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCTCGACGCT  >  CP009273/1986144‑1986432
                                                                                                                                                 |                                                                                                                                               
 gAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATaaaa                                                                                                                                              <  2:2815385/148‑1 (MQ=255)
 gAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTaaa                                                                                                                                              <  2:3374146/148‑1 (MQ=255)
      ccATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGCATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCa                                                                                                                                         <  2:323658/148‑1 (MQ=255)
      ccATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCa                                                                                                                                         <  2:1993427/148‑1 (MQ=255)
       cATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCAt                                                                                                                                        >  2:2491118/1‑148 (MQ=255)
       cATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCAt                                                                                                                                        >  1:3027296/1‑148 (MQ=255)
        aTCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATa                                                                                                                                       <  1:605775/148‑1 (MQ=255)
         tCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATaa                                                                                                                                      <  2:2243443/148‑1 (MQ=255)
         tCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATaa                                                                                                                                      <  2:2724386/148‑1 (MQ=255)
           gAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATg                                                                                                                                    <  1:2017905/148‑1 (MQ=255)
             aCCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGAc                                                                                                                                  <  2:1445662/148‑1 (MQ=255)
                 gtcagtcaCCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGt                                                                                                                              >  1:2692818/1‑148 (MQ=255)
                     gtcaCCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACt                                                                                                                          >  2:895787/1‑148 (MQ=255)
                     gtcaCCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACt                                                                                                                          >  1:2547877/1‑148 (MQ=255)
                       caCCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTAt                                                                                                                        <  2:878452/148‑1 (MQ=255)
                       caCCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTAt                                                                                                                        <  2:230644/148‑1 (MQ=255)
                          cgccgcGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCtg                                                                                                                     >  2:1371359/1‑148 (MQ=255)
                            ccgcGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCtgtt                                                                                                                   <  2:2300122/148‑1 (MQ=255)
                               cgACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTc                                                                                                                <  1:260402/148‑1 (MQ=255)
                               cgACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTc                                                                                                                >  2:1474340/1‑148 (MQ=255)
                               cgACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTc                                                                                                                >  2:312807/1‑148 (MQ=255)
                                    cAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTaa                                                                                                           <  1:2544767/148‑1 (MQ=255)
                                       aaaaTGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGAt                                                                                                        >  2:3301923/1‑148 (MQ=255)
                                          aTGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTAc                                                                                                     >  2:1149775/1‑148 (MQ=255)
                                               cgcgGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTa                                                                                                <  2:716708/148‑1 (MQ=255)
                                                gcggcgGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTAt                                                                                               <  2:458765/148‑1 (MQ=255)
                                                gcggcgGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTAt                                                                                               <  2:458794/148‑1 (MQ=255)
                                                 cggcggCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATg                                                                                              <  1:2944591/148‑1 (MQ=255)
                                                  ggcggcAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGt                                                                                             <  2:1737310/148‑1 (MQ=255)
                                                  ggcggcAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGt                                                                                             <  1:2614270/148‑1 (MQ=255)
                                                      gcAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCt                                                                                         >  2:2485048/1‑148 (MQ=255)
                                                          aCGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACag                                                                                     <  2:2660926/148‑1 (MQ=255)
                                                          aCGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACag                                                                                     <  1:657417/148‑1 (MQ=255)
                                                          aCGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACag                                                                                     >  2:2734265/1‑148 (MQ=255)
                                                          aCGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACag                                                                                     >  1:3500025/1‑148 (MQ=255)
                                                             gCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAgaga                                                                                  >  1:1821376/1‑148 (MQ=255)
                                                                aCCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACAt                                                                               <  1:3097323/148‑1 (MQ=255)
                                                                 ccAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATc                                                                              <  1:60633/148‑1 (MQ=255)
                                                                 ccAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATc                                                                              >  1:917178/1‑148 (MQ=255)
                                                                   aGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATTATGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCaa                                                                            <  2:3114269/148‑1 (MQ=255)
                                                                    ggTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAAt                                                                           >  2:127834/1‑148 (MQ=255)
                                                                    ggTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAAt                                                                           <  1:2505194/148‑1 (MQ=255)
                                                                     gTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATg                                                                          >  1:38301/1‑148 (MQ=255)
                                                                       gACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGtt                                                                        >  1:3315043/1‑148 (MQ=255)
                                                                       gACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGtt                                                                        <  1:2221309/148‑1 (MQ=255)
                                                                        aCAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGttt                                                                       >  2:2915783/1‑148 (MQ=255)
                                                                        aCAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGttt                                                                       <  2:2078792/148‑1 (MQ=255)
                                                                        aCAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGttt                                                                       >  1:2351301/1‑148 (MQ=255)
                                                                          aGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCa                                                                     >  1:2159692/1‑148 (MQ=255)
                                                                             cAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACg                                                                  >  2:1252277/1‑148 (MQ=255)
                                                                               gATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGAc                                                                <  2:2501150/148‑1 (MQ=255)
                                                                               gATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGAc                                                                >  1:1555500/1‑148 (MQ=255)
                                                                                aTAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGAcc                                                               >  2:183588/1‑148 (MQ=255)
                                                                                aTAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGAcc                                                               >  2:63765/1‑148 (MQ=255)
                                                                                 tAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCa                                                              >  2:1652528/1‑148 (MQ=255)
                                                                                  aaaaGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCag                                                             >  2:3097504/1‑148 (MQ=255)
                                                                                     aGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCagaag                                                          <  2:524609/148‑1 (MQ=255)
                                                                                      gACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCagaaga                                                         >  2:3343973/1‑148 (MQ=255)
                                                                                      gACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCagaaga                                                         >  1:399330/1‑148 (MQ=255)
                                                                                      gACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCagaaga                                                         >  2:3422529/1‑148 (MQ=255)
                                                                                      gACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCagaaga                                                         >  1:552232/1‑148 (MQ=255)
                                                                                         cAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCt                                                      >  1:1738753/1‑148 (MQ=255)
                                                                                         cAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCt                                                      >  2:887322/1‑148 (MQ=255)
                                                                                          aaTACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCtt                                                     >  2:2389808/1‑148 (MQ=255)
                                                                                           aTACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCttt                                                    >  2:1252019/1‑148 (MQ=255)
                                                                                           aTACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAAAGACCAGAATATCtta                                                    >  2:1742649/1‑147 (MQ=255)
                                                                                           aTACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTGTCAACGACCCGAAGCTCttt                                                    >  1:1920452/1‑148 (MQ=255)
                                                                                                aaGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGc                                               >  1:2398542/1‑148 (MQ=255)
                                                                                                  gAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGAAGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGTCa                                             >  1:2586452/1‑148 (MQ=255)
                                                                                                   aaTGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAg                                            >  1:318172/1‑148 (MQ=255)
                                                                                                    aTGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGa                                           <  1:828022/148‑1 (MQ=255)
                                                                                                    aTGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGa                                           <  1:1102964/148‑1 (MQ=255)
                                                                                                      gggATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCATTAAGATTACCCCTATGTTGATACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGAcc                                         <  1:1545375/148‑1 (MQ=255)
                                                                                                           aaGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCg                                    >  1:2896715/1‑148 (MQ=255)
                                                                                                             ggATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGaa                                  >  2:1768989/1‑148 (MQ=255)
                                                                                                              gATGACACGGAACAGTGTAAGCGACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGaaa                                 >  1:264017/1‑148 (MQ=255)
                                                                                                              gATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCTAGACCTTGCGaaa                                 >  1:1854959/1‑148 (MQ=255)
                                                                                                              gATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGaaa                                 >  1:3206366/1‑148 (MQ=255)
                                                                                                              gATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGaaa                                 >  1:2394685/1‑148 (MQ=255)
                                                                                                                tGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATa                               <  1:2734265/148‑1 (MQ=255)
                                                                                                                 gACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATAc                              <  2:2839876/148‑1 (MQ=255)
                                                                                                                   cacGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATAccc                            >  1:2818698/1‑148 (MQ=255)
                                                                                                                   cacGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATAccc                            <  2:1869466/148‑1 (MQ=255)
                                                                                                                    acGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCg                           >  1:3267805/1‑148 (MQ=255)
                                                                                                                        aaCAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCAc                       >  1:1396147/1‑148 (MQ=255)
                                                                                                                         aCAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACt                      <  2:1880483/148‑1 (MQ=255)
                                                                                                                         aCAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACt                      <  2:1583084/148‑1 (MQ=255)
                                                                                                                           aGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACttt                    >  2:1017068/1‑148 (MQ=255)
                                                                                                                             tgtAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTg                  <  2:3500025/148‑1 (MQ=255)
                                                                                                                             tgtAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTg                  >  1:1315662/1‑148 (MQ=255)
                                                                                                                               tAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCa                >  1:3424976/1‑148 (MQ=255)
                                                                                                                               tAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCa                <  2:1517367/148‑1 (MQ=255)
                                                                                                                                aaGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCaa               >  2:1005157/1‑148 (MQ=255)
                                                                                                                                  gCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAAtt             >  2:1008398/1‑148 (MQ=255)
                                                                                                                                    aaCGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTc           <  2:975156/148‑1 (MQ=255)
                                                                                                                                    aaCGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTc           <  1:928393/148‑1 (MQ=255)
                                                                                                                                           ggATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCTCGACg    >  2:2725678/1‑148 (MQ=255)
                                                                                                                                            gATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCTCGACGc   <  2:3154720/148‑1 (MQ=255)
                                                                                                                                             atatAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCTCGACGCt  >  1:1080200/1‑148 (MQ=255)
                                                                                                                                             atatAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCTCGACGCt  >  1:1080180/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
AGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCTCGACGCT  >  CP009273/1986144‑1986432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCTCGACGCTGGC  >  CP009273/1986140‑1986435
                                                                                                                                                     |                                                                                                                                                  
TGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATA                                                                                                                                                    <  SRR19973067.3384723/150‑1 (MQ=60)
  CCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAA                                                                                                                                                  >  SRR19973067.1922766/1‑150 (MQ=60)
    AGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAAT                                                                                                                                                >  SRR19973067.2412656/1‑150 (MQ=60)
         ACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCAT                                                                                                                                           >  SRR19973067.3046571/1‑150 (MQ=60)
          CCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATA                                                                                                                                          <  SRR19973067.488826/150‑1 (MQ=60)
          CCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATA                                                                                                                                          <  SRR19973067.2894485/150‑1 (MQ=60)
           CATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAA                                                                                                                                         >  SRR19973067.181727/1‑150 (MQ=60)
            ATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAAT                                                                                                                                        <  SRR19973067.609652/150‑1 (MQ=60)
            ATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAAT                                                                                                                                        <  SRR19973067.609322/150‑1 (MQ=60)
            ATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAAT                                                                                                                                        <  SRR19973067.609647/150‑1 (MQ=60)
               GAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGAC                                                                                                                                     <  SRR19973067.2031466/150‑1 (MQ=60)
                   CAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGT                                                                                                                                 <  SRR19973067.1540420/150‑1 (MQ=60)
                   CAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGT                                                                                                                                 >  SRR19973067.2710129/1‑150 (MQ=60)
                   CAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGT                                                                                                                                 <  SRR19973067.1865177/150‑1 (MQ=60)
                       CAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGGTGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACT                                                                                                                             >  SRR19973067.2565087/1‑150 (MQ=60)
                       CAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACT                                                                                                                             >  SRR19973067.2564545/1‑150 (MQ=60)
                                   CGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAG                                                                                                                 <  SRR19973067.262166/150‑1 (MQ=60)
                                   CGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAG                                                                                                                 <  SRR19973067.262178/150‑1 (MQ=60)
                                    GACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGT                                                                                                                >  SRR19973067.3515744/1‑150 (MQ=60)
                                     ACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTA                                                                                                               <  SRR19973067.2361889/150‑1 (MQ=60)
                                        CAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGA                                                                                                            <  SRR19973067.2561420/150‑1 (MQ=60)
                                          GAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATT                                                                                                          <  SRR19973067.369393/150‑1 (MQ=60)
                                          GAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATT                                                                                                          >  SRR19973067.2506899/1‑150 (MQ=60)
                                            AAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTAC                                                                                                        <  SRR19973067.2960209/150‑1 (MQ=60)
                                               TGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCC                                                                                                     <  SRR19973067.308960/150‑1 (MQ=60)
                                                     CGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTT                                                                                               <  SRR19973067.2963241/150‑1 (MQ=60)
                                                      GGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTG                                                                                              <  SRR19973067.2631233/150‑1 (MQ=60)
                                                         GGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTA                                                                                           >  SRR19973067.1006718/1‑150 (MQ=60)
                                                            AAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAG                                                                                        >  SRR19973067.2726729/1‑150 (MQ=60)
                                                            AAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAG                                                                                        >  SRR19973067.3522219/1‑150 (MQ=60)
                                                              ACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAG                                                                                      <  SRR19973067.661887/150‑1 (MQ=60)
                                                              ACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAG                                                                                      <  SRR19973067.661696/150‑1 (MQ=60)
                                                               CGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGA                                                                                     >  SRR19973067.1833718/1‑150 (MQ=60)
                                                                   GACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATC                                                                                 >  SRR19973067.923303/1‑150 (MQ=60)
                                                                    ACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCA                                                                                <  SRR19973067.3117027/150‑1 (MQ=60)
                                                                     CCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAA                                                                               <  SRR19973067.61368/150‑1 (MQ=60)
                                                                       AGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATG                                                                             >  SRR19973067.3276199/1‑150 (MQ=60)
                                                                       AGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATG                                                                             >  SRR19973067.38847/1‑150 (MQ=60)
                                                                        GGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGT                                                                            <  SRR19973067.2521634/150‑1 (MQ=60)
                                                                         GTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTT                                                                           >  SRR19973067.3336003/1‑150 (MQ=60)
                                                                          TGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTT                                                                          >  SRR19973067.2366651/1‑150 (MQ=60)
                                                                           GACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTC                                                                         <  SRR19973067.2236093/150‑1 (MQ=60)
                                                                            ACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCA                                                                        >  SRR19973067.2174129/1‑150 (MQ=60)
                                                                                 CAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGAC                                                                   >  SRR19973067.1566097/1‑150 (MQ=60)
                                                                                    ATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAG                                                                >  SRR19973067.1091642/1‑150 (MQ=60)
                                                                                       AAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAG                                                             >  SRR19973067.2368673/1‑150 (MQ=60)
                                                                                        AAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGA                                                            >  SRR19973067.402119/1‑150 (MQ=60)
                                                                                        AAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGA                                                            >  SRR19973067.555865/1‑150 (MQ=60)
                                                                                           ACCAATACAAAGAATGGTATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCT                                                         <  SRR19973067.2540113/150‑1 (MQ=60)
                                                                                           ACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCT                                                         <  SRR19973067.1342908/150‑1 (MQ=60)
                                                                                           ACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCT                                                         >  SRR19973067.747264/1‑150 (MQ=60)
                                                                                           ACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCT                                                         >  SRR19973067.1750642/1‑150 (MQ=60)
                                                                                             CAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTGTCAACGACCCGAAGCTCTTT                                                       >  SRR19973067.1933555/1‑150 (MQ=60)
                                                                                                  CAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGC                                                  >  SRR19973067.2414175/1‑150 (MQ=60)
                                                                                                    AAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGAAGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGTCA                                                >  SRR19973067.2603293/1‑150 (MQ=60)
                                                                                                     AGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAG                                               >  SRR19973067.320317/1‑150 (MQ=60)
                                                                                                     AGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAG                                               >  SRR19973067.320319/1‑150 (MQ=60)
                                                                                                      GAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTGCTGCCAGA                                              >  SRR19973067.1946948/1‑150 (MQ=60)
                                                                                                        ATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACC                                            <  SRR19973067.2625281/150‑1 (MQ=60)
                                                                                                        ATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACC                                            <  SRR19973067.833671/150‑1 (MQ=60)
                                                                                                        ATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACC                                            <  SRR19973067.1110465/150‑1 (MQ=60)
                                                                                                          GGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCATTAAGATTACCCCTATGTTGATACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTT                                          <  SRR19973067.1555874/150‑1 (MQ=60)
                                                                                                          GGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTT                                          <  SRR19973067.1555888/150‑1 (MQ=60)
                                                                                                            GATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGC                                        >  SRR19973067.268296/1‑150 (MQ=60)
                                                                                                             ATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCG                                       >  SRR19973067.2915201/1‑150 (MQ=60)
                                                                                                                AGGATGACACGGAACAGTGTAAGCGACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAA                                    >  SRR19973067.265810/1‑150 (MQ=60)
                                                                                                                AGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCTAGACCTTGCGAAA                                    >  SRR19973067.1867600/1‑150 (MQ=60)
                                                                                                                AGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAA                                    >  SRR19973067.2410278/1‑150 (MQ=60)
                                                                                                                AGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAA                                    >  SRR19973067.265781/1‑150 (MQ=60)
                                                                                                                AGGATGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAA                                    >  SRR19973067.3226577/1‑150 (MQ=60)
                                                                                                                    TGACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACC                                <  SRR19973067.2751912/150‑1 (MQ=60)
                                                                                                                     GACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTTCCAGACCTTGCGAAATACCC                               >  SRR19973067.2097114/1‑150 (MQ=60)
                                                                                                                     GACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCC                               >  SRR19973067.2836845/1‑150 (MQ=60)
                                                                                                                      ACACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCG                              >  SRR19973067.3288194/1‑150 (MQ=60)
                                                                                                                        ACGGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGC                            >  SRR19973067.3334492/1‑150 (MQ=60)
                                                                                                                          GGAACAGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCAC                          >  SRR19973067.1405628/1‑150 (MQ=60)
                                                                                                                               AGTGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTG                     >  SRR19973067.1324646/1‑150 (MQ=60)
                                                                                                                                 TGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCA                   >  SRR19973067.3446773/1‑150 (MQ=60)
                                                                                                                                 TGTAAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCA                   >  SRR19973067.3446606/1‑150 (MQ=60)
                                                                                                                                  gtaataacCGTAGGGATATAAAATTGAATAATGACGGGTAACTATCTGTTGTCAGTAATATTACCCCTATGTTGCTACAGATACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAA                  <  SRR19973067.2431839/142‑1 (MQ=60)
                                                                                                                                    AAGCAACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATT                >  SRR19973067.1312259/1‑150 (MQ=60)
                                                                                                                                        AACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCT            <  SRR19973067.934668/150‑1 (MQ=60)
                                                                                                                                        AACGTAGGGAGATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCT            <  SRR19973067.934612/150‑1 (MQ=60)
                                                                                                                                         ACGTAGGGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCTC           >  SRR19973067.2993132/1‑150 (MQ=60)
                                                                                                                                               GGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCTCGACGCT     >  SRR19973067.1087594/1‑150 (MQ=60)
                                                                                                                                               GGATATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCTCGACGCT     >  SRR19973067.1087574/1‑150 (MQ=60)
                                                                                                                                                  TATAAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCTCGACGCTGGC  >  SRR19973067.1181696/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                  
TGCCAGAAAACCATCGAACCAGTCAGTCACCGCCGCGACGCAGAAAATGAGCGCGGCGGCAAACGGCGACCAGGTGACAGGCAGATAAAAGACCAATACAAAGAATGGGATAAGGATGACACGGAACAGTGTAAGCAACGTAGGGATATTAAATTGCATAATGACGGGTAACTATCTGTTGTCAGTAAGATTACCCCTATGTTGCTACAGAGACATCAATGTTTCAACGACCAGAAGATCTTTTCTGCCAGACCTTGCGAAATACCCGGCACTTTTGCAATTTCCTCGACGCTGGC  >  CP009273/1986140‑1986435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑