Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F86 I0 R1	593	297.4	9515568	94.4%	8982696	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,411,456	C→A	10.5%	P1022Q (CCG→CAG)	acrF →	multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,411,456	0	C	A	10.5%	967.4 / 62.8	273	P1022Q (CCG→CAG)	acrF	multidrug efflux system protein
Reads supporting (aligned to +/- strand): ref base C (101/143); new base A (13/16); total (114/159)
Fisher's exact test for biased strand distribution p-value = 8.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 275 total aligned reads displayed.

TCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCTTTCTATAGTTCC  >  CP009273/3411307‑3411603

tcCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTAcc                                                                                                                                                     <  1:1075510/150‑1 (MQ=255)

   tATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGgt                                                                                                                                                  <  1:3300095/150‑1 (MQ=255)

   tATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGgt                                                                                                                                                  >  2:2312246/1‑150 (MQ=255)

   tATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGgt                                                                                                                                                  <  2:679617/150‑1 (MQ=255)

   tATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACAGgt                                                                                                                                                  >  1:3876126/1‑150 (MQ=255)

     tCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGgtgt                                                                                                                                                <  2:1103336/150‑1 (MQ=255)

      ccTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGtt                                                                                                                                               <  2:3213430/150‑1 (MQ=255)

       cTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTc                                                                                                                                              <  2:4247135/150‑1 (MQ=255)

        tgatgaCCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCt                                                                                                                                             >  2:276189/1‑150 (MQ=255)

          atgaCCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCttt                                                                                                                                           <  1:1033332/150‑1 (MQ=255)

          atgaCCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCttt                                                                                                                                           <  1:3805965/150‑1 (MQ=255)

           tgaCCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTtg                                                                                                                                          >  1:112610/1‑150 (MQ=255)

            gaCCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACAGGTGTTCTTtgt                                                                                                                                         >  2:58409/1‑150 (MQ=255)

            gaCCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACAGGTGTTCTTtgt                                                                                                                                         >  2:3452029/1‑150 (MQ=255)

             aCCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTtgtg                                                                                                                                        <  1:1406257/150‑1 (MQ=255)

               ctctctCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTgtggt                                                                                                                                      >  1:3469654/1‑150 (MQ=255)

               ctctctCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTgtggt                                                                                                                                      <  2:1736319/150‑1 (MQ=255)

                tctctcGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTgtggtg                                                                                                                                     <  1:922703/150‑1 (MQ=255)

                 ctctcGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGa                                                                                                                                    >  1:4652233/1‑150 (MQ=255)

                  tctcGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCCGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGAt                                                                                                                                   >  2:2470837/1‑150 (MQ=255)

                   ctcGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTCACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATc                                                                                                                                  >  1:1594190/1‑150 (MQ=255)

                    tcGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATcc                                                                                                                                 <  1:2694807/150‑1 (MQ=255)

                    tcGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACAGGTGTTCTTTGTGGTGATcc                                                                                                                                 <  1:3673523/150‑1 (MQ=255)

                      gCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATccgc                                                                                                                               >  1:3133651/1‑150 (MQ=255)

                       ccTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATccgcc                                                                                                                              >  1:489498/1‑150 (MQ=255)

                       ccTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATccgcc                                                                                                                              >  1:17543/1‑150 (MQ=255)

                         tttATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGACTGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGt                                                                                                                            >  1:1257288/1‑150 (MQ=255)

                         tttATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGt                                                                                                                            >  1:4584994/1‑150 (MQ=255)

                          ttATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGtt                                                                                                                           <  1:2748726/150‑1 (MQ=255)

                          ttATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGtt                                                                                                                           <  1:2337965/150‑1 (MQ=255)

                           tATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCTGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACAGGTGTTCTTTGTGGTGATCCGCCGTTg                                                                                                                          >  1:4174183/1‑150 (MQ=255)

                           tATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTg                                                                                                                          >  2:4667837/1‑150 (MQ=255)

                             ttCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCt                                                                                                                        <  1:24416/150‑1 (MQ=255)

                               ctcGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCttt                                                                                                                      <  1:3518352/150‑1 (MQ=255)

                                tcGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTa                                                                                                                     <  1:42888/150‑1 (MQ=255)

                                  ggCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTaaa                                                                                                                   <  2:2884054/150‑1 (MQ=255)

                                  ggCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACAGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTaaa                                                                                                                   <  2:2343956/150‑1 (MQ=255)

                                      tATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGAt                                                                                                               >  1:4066441/1‑150 (MQ=255)

                                      tATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGAt                                                                                                               <  1:2768879/150‑1 (MQ=255)

                                            cGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCa                                                                                                         >  2:2126883/1‑150 (MQ=255)

                                            cGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCa                                                                                                         >  2:796543/1‑150 (MQ=255)

                                                agctaTCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGaaa                                                                                                     <  2:1025392/150‑1 (MQ=255)

                                                   taTCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACAt                                                                                                  >  2:1838293/1‑150 (MQ=255)

                                                        gTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAgg                                                                                             <  2:1450719/150‑1 (MQ=255)

                                                          aaCGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGgcg                                                                                           <  2:3654119/150‑1 (MQ=255)

                                                          aaCGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGgcg                                                                                           >  2:307979/1‑150 (MQ=255)

                                                              gTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCttt                                                                                       <  1:1897983/150‑1 (MQ=255)

                                                               tGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTc                                                                                      >  1:1994507/1‑150 (MQ=255)

                                                                gCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGGTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCg                                                                                     <  2:2714219/150‑1 (MQ=255)

                                                                 ccGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACAGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCgg                                                                                    <  2:2578579/150‑1 (MQ=255)

                                                                  cGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCggg                                                                                   >  1:250251/1‑150 (MQ=255)

                                                                   ggCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGt                                                                                  >  2:2655702/1‑150 (MQ=255)

                                                                       gTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCt                                                                              <  1:3807383/150‑1 (MQ=255)

                                                                        tGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCtt                                                                             >  1:1178967/1‑150 (MQ=255)

                                                                           cgcgcAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTttat                                                                          <  2:4384148/150‑1 (MQ=255)

                                                                           cgcgcAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTttat                                                                          <  2:3942013/150‑1 (MQ=255)

                                                                             cgcAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTttatta                                                                        <  2:3669910/150‑1 (MQ=255)

                                                                              gcAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTtattat                                                                       <  1:1334406/150‑1 (MQ=255)

                                                                                aGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTAttt                                                                     >  1:3361682/1‑150 (MQ=255)

                                                                                   aCGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCa                                                                  <  1:402388/150‑1 (MQ=255)

                                                                                       tgtgGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGa                                                              >  2:4323768/1‑150 (MQ=255)

                                                                                        gtgGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGaa                                                             <  2:360595/150‑1 (MQ=255)

                                                                                         tgGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGaaa                                                            >  1:2937564/1‑150 (MQ=255)

                                                                                         tgGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGaaa                                                            >  2:4071626/1‑150 (MQ=255)

                                                                                            gTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAAccc                                                         <  2:716708/150‑1 (MQ=255)

                                                                                            gTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAAccc                                                         >  2:2930986/1‑150 (MQ=255)

                                                                                              aTCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCAt                                                       <  2:2644522/150‑1 (MQ=255)

                                                                                              aTCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCAt                                                       <  1:654789/150‑1 (MQ=255)

                                                                                               tCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATa                                                      <  2:3820297/150‑1 (MQ=255)

                                                                                               tCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATa                                                      <  2:2410951/150‑1 (MQ=255)

                                                                                               tCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATa                                                      <  2:1997461/150‑1 (MQ=255)

                                                                                               tCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATa                                                      <  2:3411636/150‑1 (MQ=255)

                                                                                                 ggggTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATaaa                                                    <  1:4493101/150‑1 (MQ=255)

                                                                                                   ggTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTACAGTGAAACCCATaaaaa                                                  >  1:3213132/1‑150 (MQ=255)

                                                                                                          ggaggaATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATtaataa                                           >  2:1340025/1‑150 (MQ=255)

                                                                                                            aggaATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGa                                         >  2:690002/1‑150 (MQ=255)

                                                                                                            aggaATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGa                                         <  2:2507099/150‑1 (MQ=255)

                                                                                                            aggaATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACAGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCATTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGa                                         <  2:1898666/150‑1 (MQ=255)

                                                                                                             ggaATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGat                                        <  1:1600273/150‑1 (MQ=255)

                                                                                                             ggaATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGat                                        <  2:422126/150‑1 (MQ=255)

                                                                                                               aaTGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGatat                                      <  2:2266190/150‑1 (MQ=255)

                                                                                                               aaTGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGatat                                      >  1:2126646/1‑150 (MQ=255)

                                                                                                               aaTGGTCTCAGCAACGTTGCTGGCAATCTTCTACGTACCGGTGATCTTTTTGGTGATCCGCAGTTGCTTTAAAGGATAAATCAGAAACATAAAGTCGCTTTCGGGTCCCTTTATTATTTCCAGTGAAACACAAAAAAATTAATAAGatat                                      >  2:4404792/1‑150 (MQ=255)

                                                                                                                  ggTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACAGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATAttct                                   <  1:1868983/150‑1 (MQ=255)

                                                                                                                   gTCTCTGCAACGTTGCTGGCTATCTTCTTCGTACAGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATAttctt                                  <  2:4597863/150‑1 (MQ=255)

                                                                                                                   gTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACAGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATAttctt                                  <  2:3875969/150‑1 (MQ=255)

                                                                                                                      tctGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTg                               <  1:262636/150‑1 (MQ=255)

                                                                                                                         gCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTc                            >  1:4223623/1‑150 (MQ=255)

                                                                                                                          cAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCa                           <  2:4091824/150‑1 (MQ=255)

                                                                                                                            aCGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCAct                         >  2:2032052/1‑150 (MQ=255)

                                                                                                                              gTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCActct                       >  1:1687060/1‑150 (MQ=255)

                                                                                                                                    tgaaaTCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGATTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTaaaa                 <  2:4533879/147‑1 (MQ=255)

                                                                                                                                        aTCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCtt             >  2:4296787/1‑150 (MQ=255)

                                                                                                                                        aTCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCtt             >  2:1509772/1‑150 (MQ=255)

                                                                                                                                        aTCTTCTTCGTACAGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCtt             >  2:2634001/1‑150 (MQ=255)

                                                                                                                                         tcttctTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCttt            <  2:511825/150‑1 (MQ=255)

                                                                                                                                              ttcGTACCGGTGTTCTTGGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCTTTCtata       <  2:4386434/150‑1 (MQ=255)

                                                                                                                                              ttcGTACAGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCTTTCtata       <  2:3527988/150‑1 (MQ=255)

                                                                                                                                               tcGTACAGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCTTTCTATAg      <  2:3923991/150‑1 (MQ=255)

                                                                                                                                                  tACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCTTTCTATAGTTc   >  1:1515216/1‑150 (MQ=255)

TCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCTTTCTATAGTTCC  >  CP009273/3411307‑3411603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A