Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F78 I0 R1	626	241.3	10389380	77.7%	8072548	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,411,456	C→A	27.9%	P1022Q (CCG→CAG)	acrF →	multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,411,456	0	C	A	27.9%	460.7 / 179.8	228	P1022Q (CCG→CAG)	acrF	multidrug efflux system protein
Reads supporting (aligned to +/- strand): ref base C (77/87); new base A (35/29); total (112/116)
Fisher's exact test for biased strand distribution p-value = 3.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 231 total aligned reads displayed.

TCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCTTTCTATAGTTCC  >  CP009273/3411307‑3411603

tcCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTAcc                                                                                                                                                     <  2:661402/150‑1 (MQ=255)

 ccTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCGGGCAATCTTCTTCGTACCg                                                                                                                                                    <  2:2674874/150‑1 (MQ=255)

  cTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGACTGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCgg                                                                                                                                                   >  1:2956515/1‑150 (MQ=255)

    aTCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTCTCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGgtg                                                                                                                                                 >  1:3973275/1‑150 (MQ=255)

    aTCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGgtg                                                                                                                                                 >  1:794580/1‑150 (MQ=255)

       cTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTc                                                                                                                                              <  1:826828/150‑1 (MQ=255)

       cTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTc                                                                                                                                              <  1:3430254/150‑1 (MQ=255)

        tgatgaCCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCt                                                                                                                                             <  2:1434592/150‑1 (MQ=255)

        tgatgaCCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACAGGTGTTCt                                                                                                                                             >  1:867047/1‑150 (MQ=255)

        tgatgaCCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGAGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCt                                                                                                                                             >  2:1756595/1‑150 (MQ=255)

         gatgaCCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCtt                                                                                                                                            >  1:573625/1‑150 (MQ=255)

         gatgaCCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCtt                                                                                                                                            >  1:1657914/1‑150 (MQ=255)

               ctctctCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTgtggt                                                                                                                                      >  1:1219322/1‑150 (MQ=255)

                 ctctcGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACAGGTGTTCTTTGTGGTGa                                                                                                                                    >  1:4860987/1‑150 (MQ=255)

                 ctctcGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACAGGTGTTCTTTGTGGTGa                                                                                                                                    >  1:1040402/1‑150 (MQ=255)

                  tctcGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACAGGTGTTCTTTGTGGTGAt                                                                                                                                   >  2:705036/1‑150 (MQ=255)

                   ctcGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACAGGTGTTCTTTGTGGTGATc                                                                                                                                  >  2:4023497/1‑150 (MQ=255)

                   ctcGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACAGGTGTTCTTTGTGGTGATc                                                                                                                                  <  1:1775111/150‑1 (MQ=255)

                    tcGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACAGGTGTTCTTTGTGGTGATcc                                                                                                                                 <  1:1358085/150‑1 (MQ=255)

                        cTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATccgccg                                                                                                                             <  1:4727864/150‑1 (MQ=255)

                        cTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACAGGTGTTCTTTGTGGTGATccgccg                                                                                                                             >  2:2220602/1‑150 (MQ=255)

                           tATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTg                                                                                                                          <  2:3862684/150‑1 (MQ=255)

                           tATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACAGGTGTTCTTTGTGGTGATCCGCCGTTg                                                                                                                          >  2:1603411/1‑150 (MQ=255)

                              tctcGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCtt                                                                                                                       <  2:2323698/150‑1 (MQ=255)

                              tctcGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCtt                                                                                                                       <  1:4521132/150‑1 (MQ=255)

                               ctcGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCttt                                                                                                                      <  2:2881144/150‑1 (MQ=255)

                               ctcGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCGGGCAATCTTCTTCGTACAGGTGTTCTTTGTGGTGATCCGCCGTTGCttt                                                                                                                      <  2:3269843/150‑1 (MQ=255)

                                 cGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTaa                                                                                                                    <  2:2794095/150‑1 (MQ=255)

                                   gCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAg                                                                                                                  <  1:2249619/150‑1 (MQ=255)

                                   gCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAg                                                                                                                  <  1:2497806/150‑1 (MQ=255)

                                     gTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGa                                                                                                                >  2:3626795/1‑150 (MQ=255)

                                     gTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACAGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGa                                                                                                                >  2:4937034/1‑150 (MQ=255)

                                      tATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGAt                                                                                                               <  1:4845068/150‑1 (MQ=255)

                                       aTTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACAGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATa                                                                                                              >  1:4139800/1‑150 (MQ=255)

                                          aCCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAAt                                                                                                           <  1:3780908/150‑1 (MQ=255)

                                           ccGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACAGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATc                                                                                                          >  2:3729444/1‑150 (MQ=255)

                                             gctagctaTCAGTAAGGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACAGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAg                                                                                                        <  2:4641602/150‑1 (MQ=255)

                                             gctagctaTCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACAGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAg                                                                                                        <  2:3799227/150‑1 (MQ=255)

                                              ctagctaTCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCACCGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTGTAAAGGATAAATCAGa                                                                                                       >  2:4344444/1‑150 (MQ=255)

                                               tagctaTCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGaa                                                                                                      >  1:5079893/1‑150 (MQ=255)

                                                agctaTCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGaaa                                                                                                     >  1:4456616/1‑150 (MQ=255)

                                                      cAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATaaa                                                                                               <  1:4699058/150‑1 (MQ=255)

                                                       aGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAg                                                                                              <  1:1541156/150‑1 (MQ=255)

                                                       aGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACAGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAg                                                                                              <  2:3690514/150‑1 (MQ=255)

                                                         tAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACAGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGgc                                                                                            <  1:1988697/150‑1 (MQ=255)

                                                           aCGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACAGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGgcgc                                                                                          <  1:704886/150‑1 (MQ=255)

                                                             ggTGTCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTGATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCtt                                                                                        >  2:1942718/1‑150 (MQ=255)

                                                             ggTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCtt                                                                                        >  1:2012467/1‑150 (MQ=255)

                                                               tGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTc                                                                                      <  2:4597074/150‑1 (MQ=255)

                                                                gCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTGTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCg                                                                                     >  2:3966384/1‑150 (MQ=255)

                                                                        tGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCtt                                                                             >  1:1637243/1‑150 (MQ=255)

                                                                         ggCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACAGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCttt                                                                            <  2:4000999/150‑1 (MQ=255)

                                                                           cgcgcAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTttat                                                                          >  1:78810/1‑150 (MQ=255)

                                                                             cgcAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTttatta                                                                        >  2:1925142/1‑150 (MQ=255)

                                                                              gcAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTtattat                                                                       >  2:1468656/1‑150 (MQ=255)

                                                                                  aaCGCTGTGGGTATCGGGGTAATGGGAGGACTGGTATCTGCAACGTTGCTGGCAATCTTCTTCGTACAGGTGATCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTAGGGTGCCTTTATTAATTcc                                                                   >  2:2638974/1‑150 (MQ=255)

                                                                                  aaCGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACAGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTcc                                                                   <  1:3932382/150‑1 (MQ=255)

                                                                                    cGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAg                                                                 <  1:4998824/150‑1 (MQ=255)

                                                                                    cGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCGGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAg                                                                 <  2:4821482/150‑1 (MQ=255)

                                                                                       tgtgGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGa                                                              <  2:3087059/150‑1 (MQ=255)

                                                                                          gggTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAAc                                                           <  1:3747680/150‑1 (MQ=255)

                                                                                           ggTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACAGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAAcc                                                          >  1:1474936/1‑150 (MQ=255)

                                                                                                 ggggTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATaaa                                                    <  1:1194910/150‑1 (MQ=255)

                                                                                                   ggTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATaaaaa                                                  >  1:1175369/1‑150 (MQ=255)

                                                                                                    gTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAAt                                                 >  2:5087025/1‑150 (MQ=255)

                                                                                                    gTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAAt                                                 <  1:1549399/150‑1 (MQ=255)

                                                                                                     tAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAAtt                                                >  2:3546456/1‑150 (MQ=255)

                                                                                                     tAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAAtt                                                >  1:201940/1‑150 (MQ=255)

                                                                                                      aaTGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATta                                               >  1:4747669/1‑150 (MQ=255)

                                                                                                          ggaggaATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACAGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATtaataa                                           >  2:573129/1‑150 (MQ=255)

                                                                                                           taacatcggTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAg                                          >  2:3222157/8‑150 (MQ=255)

                                                                                                               aaTGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGatat                                      >  2:809528/1‑150 (MQ=255)

                                                                                                                aTGGTCTGTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGTCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACACATAAAAATTAATAAGATAtt                                     >  2:2967100/1‑150 (MQ=255)

                                                                                                                aTGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATAtt                                     >  2:1287657/1‑150 (MQ=255)

                                                                                                                aTGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATAtt                                     <  1:4090879/150‑1 (MQ=255)

                                                                                                                 tGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACAGGTGTTCTCTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATAttc                                    <  2:405631/150‑1 (MQ=255)

                                                                                                                   gTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATAttctt                                  <  1:981547/150‑1 (MQ=255)

                                                                                                                   gTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACAGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATAttctt                                  >  1:2478619/1‑150 (MQ=255)

                                                                                                                    tctcTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATAttcttc                                 <  1:2304703/150‑1 (MQ=255)

                                                                                                                    tctcTGCAACGTTGCTGGCAATCTTCTTCGTACAGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATAttcttc                                 >  2:2191960/1‑150 (MQ=255)

                                                                                                                    tctcTGCAACGTTGCTGGCAATCTTCTTCGTACAGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATAttcttc                                 <  2:4985383/150‑1 (MQ=255)

                                                                                                                     ctctGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATtcttct                                >  2:1303188/1‑150 (MQ=255)

                                                                                                                      tctGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTg                               >  2:4864368/1‑150 (MQ=255)

                                                                                                                          cAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCa                           >  2:1871357/1‑150 (MQ=255)

                                                                                                                           aaCGTTGCTGGCAATCTTCTTCGTACAGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCAc                          >  1:2528892/1‑150 (MQ=255)

                                                                                                                            aCGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCAct                         <  2:1108446/150‑1 (MQ=255)

                                                                                                                              gTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCActct                       >  1:4333414/1‑150 (MQ=255)

                                                                                                                                     gCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAg                <  1:1247292/150‑1 (MQ=255)

                                                                                                                                      cAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGc               >  2:3521879/1‑150 (MQ=255)

                                                                                                                                       aaTCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCt              >  1:3004258/1‑150 (MQ=255)

                                                                                                                                          cttcttCGTACAGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCTTTc           <  1:5122647/150‑1 (MQ=255)

                                                                                                                                           ttcttcGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCTTTCt          <  1:1437127/150‑1 (MQ=255)

                                                                                                                                           ttcttcGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCTTTCt          <  2:1501830/150‑1 (MQ=255)

                                                                                                                                           ttcttcGTACAGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCTTTCt          >  1:4060021/1‑150 (MQ=255)

                                                                                                                                            tcttcGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCTTTCta         <  2:3172533/150‑1 (MQ=255)

                                                                                                                                             cttcGTACAGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCTTTCtat        >  2:4033766/1‑150 (MQ=255)

                                                                                                                                             cttcGTACAGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCTTTCtat        >  2:917482/1‑150 (MQ=255)

                                                                                                                                               tcGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCTTTCTATAg      <  1:2095571/150‑1 (MQ=255)

                                                                                                                                                cGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCTTTCTATAGt     <  1:2834505/150‑1 (MQ=255)

                                                                                                                                                  tACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTATTATGCTCACTCTTTAAAAGCTTTCTATAGTTc   >  2:3172486/1‑150 (MQ=255)

TCCTATCCTGATGACCTCTCTCGCCTTTATTCTCGGCGTATTACCGCTAGCTATCAGTAACGGTGCCGGCAGTGGCGCGCAGAACGCTGTGGGTATCGGGGTAATGGGAGGAATGGTCTCTGCAACGTTGCTGGCAATCTTCTTCGTACCGGTGTTCTTTGTGGTGATCCGCCGTTGCTTTAAAGGATAAATCAGAAACATAAAGGCGCTTTCGGGTGCCTTTATTATTTCCAGTGAAACCCATAAAAATTAATAAGATATTCTTCTGCTCACTCTTTAAAAGCTTTCTATAGTTCC  >  CP009273/3411307‑3411603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A