breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 5,144 G→A 87.8% intergenic (+124/‑90) thrC → / → yaaX L‑threonine synthase/DUF2502 family putative periplasmic protein
RA 10,444 G→T 100% F17L (TTC→TTA satP ← succinate‑acetate transporter
RA 12,901 G→A 100% D247N (GAT→AAT)  dnaK → chaperone Hsp70, with co‑chaperone DnaJ
RA 24,720 A→C 6.4% Y777S (TAC→TCC)  ileS → isoleucyl‑tRNA synthetase
RA 25,013 G→T 15.4% D875Y (GAC→TAC)  ileS → isoleucyl‑tRNA synthetase
RA 25,546 G→T 31.7% D114Y (GAC→TAC)  lspA → prolipoprotein signal peptidase (signal peptidase II)
RA 29,139 G→T 84.2% G256C (GGT→TGT)  dapB → dihydrodipicolinate reductase
RA 32,178 C→A 7.1% N454K (AAC→AAA carB → carbamoyl‑phosphate synthase large subunit
RA 45,406 C→A 6.3% G409G (GGC→GGA fixC → putative oxidoreductase
RA 49,840 G→T 61.7% A6A (GCG→GCT folA → dihydrofolate reductase
RA 51,607 C→A 13.1% intergenic (‑1/+2) apaG ← / ← rsmA protein associated with Co2+ and Mg2+ efflux/16S rRNA m(6)A1518, m(6)A1519 dimethyltransferase, SAM‑dependent
RA 54,650 C→T 8.2% S18N (AGT→AAT)  surA ← peptidyl‑prolyl cis‑trans isomerase (PPIase)
RA 55,590 G→T 37.1% T507K (ACG→AAG)  lptD ← LPS assembly OM complex LptDE, beta‑barrel component
RA 56,677 G→T 80.0% R145S (CGC→AGC)  lptD ← LPS assembly OM complex LptDE, beta‑barrel component
RA 72,337 C→A 9.0% D484Y (GAC→TAC)  sgrR ← transcriptional DNA‑binding transcriptional activator of sgrS sRNA
RA 72,392 G→T 9.8% F465L (TTC→TTA sgrR ← transcriptional DNA‑binding transcriptional activator of sgrS sRNA
RA 79,882 G→T 21.9% V45V (GTC→GTA leuA ← 2‑isopropylmalate synthase
RA 80,123 C→T 31.7% G25S (GGC→AGC)  leuL ← leu operon leader peptide
RA 81,562 C→A 40.5% I236I (ATC→ATA leuO → global transcription factor
RA 89,009 G→T 6.8% A370A (GCG→GCT ftsI → transpeptidase involved in septal peptidoglycan synthesis (penicillin‑binding protein 3)
RA 97,521 C→A 64.7% A90A (GCC→GCA murC → UDP‑N‑acetylmuramate:L‑alanine ligase
RA 103,467 G→T 72.6% D142Y (GAT→TAT)  lpxC → UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase
RA 104,561 G→T 74.3% A124S (GCG→TCG)  secM → regulator of secA translation
RA 104,948 A→T 16.3% E61D (GAA→GAT secA → preprotein translocase subunit, ATPase
RA 109,800 A→T 5.0% intergenic (‑94/‑131) coaE ← / → guaC dephospho‑CoA kinase/GMP reductase
RA 118,873 G→T 100% D99Y (GAC→TAC)  pdhR → pyruvate dehydrogenase complex repressor; autorepressor
RA 123,870 G→T 12.7% P563P (CCG→CCT aceF → pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
RA 132,632 A→T 20.8% V107E (GTG→GAG)  speE ← spermidine synthase (putrescine aminopropyltransferase)
RA 142,460 T→A 92.2% F298Y (TTC→TAC)  yadE → putative polysaccharide deacetylase lipoprotein
RA 147,946 G→T 12.2% I47I (ATC→ATA yadK ← putative fimbrial‑like adhesin protein
RA 153,472 G→T 90.7% intergenic (‑102/+268) yadN ← / ← folK putative fimbrial‑like adhesin protein/2‑amino‑4‑hydroxy‑6‑hydroxymethyldihyropteridine pyrophosphokinase
RA 157,178 C→A 14.9% intergenic (‑87/+91) dksA ← / ← sfsA transcriptional regulator of rRNA transcription, DnaK suppressor protein/sugar fermentation stimulation protein A
RA 158,287 C→A 90.2% D78Y (GAC→TAC)  ligT ← 2'‑5' RNA ligase
RA 161,068 A→C 32.0% intergenic (+47/‑149) hrpB → / → mrcB putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase
RA 169,148 G→T 8.8% A400A (GCG→GCT fhuB → fused iron‑hydroxamate transporter subunits of ABC superfamily: membrane components
RA 170,868 C→A 11.1% V168F (GTT→TTT)  hemL ← glutamate‑1‑semialdehyde aminotransferase (aminomutase)
RA 192,769 A→C 7.3% V202V (GTA→GTC cdsA → CDP‑diglyceride synthase
RA 198,022 C→A 19.6% R189S (CGT→AGT)  lpxD → UDP‑3‑O‑(3‑hydroxymyristoyl)‑glucosamine N‑acyltransferase
RA 199,351 C→A 9.7% T102K (ACA→AAA)  lpxA → UDP‑N‑acetylglucosamine acetyltransferase
RA 209,413 A→T 7.3% Q199L (CAG→CTG)  tilS → tRNA(Ile)‑lysidine synthetase
RA 210,546 C→A 18.4% V23F (GTT→TTT)  yaeP ← UPF0253 family protein
RA 230,579 C→T 100% E231K (GAA→AAA)  gloB ← hydroxyacylglutathione hydrolase
RA 232,860 G→T 93.0% D103Y (GAT→TAT)  dnaQ → DNA polymerase III epsilon subunit
RA 234,984 G→T 16.2% I80I (ATC→ATA ykfM ← lethality reduction protein, putative inner membrane protein
RA 234,990 G→T 11.7% F78L (TTC→TTA ykfM ← lethality reduction protein, putative inner membrane protein
RA 234,994 G→T 13.5% S77* (TCA→TAA)  ykfM ← lethality reduction protein, putative inner membrane protein
RA 235,010 G→T 15.6% L72I (CTT→ATT)  ykfM ← lethality reduction protein, putative inner membrane protein
RA 235,019 C→A 15.5% V69L (GTA→TTA)  ykfM ← lethality reduction protein, putative inner membrane protein
RA 235,022 G→T 24.3% H68N (CAT→AAT)  ykfM ← lethality reduction protein, putative inner membrane protein
RA 238,195 G→T 83.3% V532V (GTC→GTA fadE ← acyl coenzyme A dehydrogenase
RA 255,882 A→T 23.9% intergenic (‑71/‑217) phoE ← / → proB outer membrane phosphoporin protein E/gamma‑glutamate kinase
RA 274,735 A→T 27.1% pseudogene (138/348 nt) afuB ← pseudogene, CP4‑6 prophage; ABC transporter permease family;Phage or Prophage Related
RA 289,058 C→T 7.7% G191E (GGA→GAA)  yagL ← CP4‑6 prophage; DNA‑binding protein
RA 295,695 T→G 18.5% E317D (GAA→GAC paoC ← PaoABC aldehyde oxidoreductase, Moco‑containing subunit
RA 312,532 G→A 91.5% pseudogene (349/684 nt) ykgA ← pseudogene, AraC family;putative regulator; Not classified; putative ARAC‑type regulatory protein
RA 320,920 G→A 83.5% S52S (AGC→AGT ykgH ← putative inner membrane protein
RA 321,223 C→A 37.9% intergenic (‑148/+65) ykgH ← / ← betA putative inner membrane protein/choline dehydrogenase, a flavoprotein
RA 327,515 G→T 24.0% intergenic (+308/‑567) betT → / → yahA choline transporter of high affinity/c‑di‑GMP‑specific phosphodiesterase
RA 334,250 G→T 10.6% R72L (CGT→CTT)  yahG → DUF1116 family protein
RA 344,800 G→T 29.7% E136D (GAG→GAT prpB → 2‑methylisocitrate lyase
RA 353,517 T→C 7.0% G295G (GGA→GGG cynR ← transcriptional activator of cyn operon; autorepressor
RA 353,537 T→C 5.2% K289E (AAA→GAA)  cynR ← transcriptional activator of cyn operon; autorepressor
RA 360,752 A→G 100% V331A (GTG→GCG)  lacI ← DNA‑binding transcriptional repressor
JC 361,139 +GCCA 9.2% coding (605/1083 nt) lacI ← DNA‑binding transcriptional repressor
RA 361,806 G→A 100% intergenic (‑63/+14) lacI ← / ← mhpR DNA‑binding transcriptional repressor/mhp operon transcriptional activator
RA 362,159 G→T 85.4% I165I (ATC→ATA mhpR ← mhp operon transcriptional activator
RA 376,532 C→A 15.3% pseudogene (184/416 nt) yaiX ← pseudogene, interrupted by IS2A, acetyltransferase homolog; nonfunctional; interruped by IS2; putative transferase
RA 380,508 C→T 6.6% intergenic (‑436/‑180) yaiS ← / → tauA putative PIG‑L family deacetylase/taurine transporter subunit
RA 389,725 G→T 11.8% pseudogene (1300/1449 nt) yaiT → pseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein
RA 396,883 A→G 32.7% Q14Q (CAA→CAG iraP → anti‑RssB factor, RpoS stabilzer during Pi starvation; anti‑adapter protein
RA 400,819 A→C 8.8% V94V (GTT→GTG proC ← pyrroline‑5‑carboxylate reductase, NAD(P)‑binding
RA 402,340 G→T 91.7% V160V (GTG→GTT aroL → shikimate kinase II
RA 406,075 C→A 100% P159Q (CCG→CAG)  mak → manno(fructo)kinase
RA 411,027 G→T 94.0% L61L (CTC→CTA sbcC ← exonuclease, dsDNA, ATP‑dependent
RA 419,663 G→T 11.2% E564* (GAA→TAA)  malZ → maltodextrin glucosidase
RA 421,799 C→A 29.2% I69I (ATC→ATA tgt → tRNA‑guanine transglycosylase
RA 424,725 A→C 6.2% A541A (GCA→GCC secD → SecYEG protein translocase auxillary subunit
RA 433,551 G→T 93.3% S14Y (TCC→TAC)  yajO ← 2‑carboxybenzaldehyde reductase
RA 434,663 A→C 95.0% I324S (ATC→AGC)  dxs ← 1‑deoxyxylulose‑5‑phosphate synthase, thiamine triphosphate‑binding, FAD‑requiring
RA 435,336 G→T 80.3% Q100K (CAG→AAG)  dxs ← 1‑deoxyxylulose‑5‑phosphate synthase, thiamine triphosphate‑binding, FAD‑requiring
RA 437,672 T→A 83.6% V223E (GTG→GAG)  thiI → tRNA s(4)U8 sulfurtransferase
RA 442,849 C→A 5.9% A105S (GCG→TCG)  cyoE ← protoheme IX farnesyltransferase
RA 444,489 G→A 64.7% R537C (CGT→TGT)  cyoB ← cytochrome o ubiquinol oxidase subunit I
RA 456,298 T→A 66.7% L652Q (CTG→CAG)  lon → DNA‑binding ATP‑dependent protease La
RA 456,588 G→T 100% D749Y (GAC→TAC)  lon → DNA‑binding ATP‑dependent protease La
RA 461,641 G→A 13.0% V376V (GTC→GTT ybaE ← putative ABC superfamily transporter periplasmic‑binding protein
RA 474,138 G→T 88.1% intergenic (‑65/+99) ylaB ← / ← ylaC putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase/DUF1449 family inner membrane protein
RA 475,805 C→A 65.6% A120A (GCG→GCT tomB ← Hha toxicity attenuator; conjugation‑related protein
RA 479,244 C→A 84.5% A206S (GCC→TCC)  acrB ← multidrug efflux system protein
RA 480,820 C→T 94.9% D86N (GAC→AAC)  acrA ← multidrug efflux system
RA 481,667 G→A 67.0% A151T (GCG→ACG)  acrR → transcriptional repressor
RA 486,169 A→T 6.9% F34I (TTC→ATC)  priC ← primosomal replication protein N''
RA 495,504 A→T 16.3% intergenic (‑75/‑77) aes ← / → gsk acetyl esterase/inosine/guanosine kinase
RA 501,656 C→A 5.3% F429L (TTC→TTA ushA → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA 507,331 G→A 17.3% D79N (GAT→AAT)  glsA → glutaminase 1
RA 509,545 G→T 9.3% E33* (GAA→TAA)  cueR → copper‑responsive regulon transcriptional regulator
RA 523,244 G→T 20.4% E70* (GAA→TAA)  ybbC → putative immunity protein
RA 530,466 C→A 93.2% F365L (TTC→TTA gcl → glyoxylate carboligase
RA 533,530 G→T 8.7% D148Y (GAT→TAT)  ybbW → putative allantoin transporter
RA 542,200 Δ1 bp 88.7% coding (65/1668 nt) fdrA → putative NAD(P)‑binding acyl‑CoA synthetase
RA 544,713 G→T 20.0% D301Y (GAT→TAT)  ylbE → NO‑induced DUF1116 protein
RA 546,491 G→T 100% V199V (GTG→GTT ybcF → putative carbonate kinase
RA 549,397 G→T 93.2% intergenic (‑1/+2) lpxH ← / ← ppiB UDP‑2,3‑diacylglucosamine pyrophosphohydrolase/peptidyl‑prolyl cis‑trans isomerase B (rotamase B)
RA 553,502 C→A 9.6% intergenic (‑305/‑166) folD ← / → sfmA bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase/ 5,10‑methylene‑tetrahydrofolate cyclohydrolase/FimA homolog
RA 565,317 G→A 7.4% A320A (GCG→GCA ybcK → DLP12 prophage; putative recombinase
RA 576,998 G→T 37.4% pseudogene (189/309 nt) aaaD → pseudogene; DLP12 prophage; tail fiber assembly protein family;Phage or Prophage Related
RA 579,710 G→A 11.9% A192T (GCA→ACA)  appY → global transcriptional activator; DLP12 prophage
RA 581,203 A→T 65.5% intergenic (‑114/‑310) ompT ← / → pauD DLP12 prophage; outer membrane protease VII (outer membrane protein 3b)/tRNA‑OTHER
RA 583,341 T→G 91.4% S33R (AGT→CGT)  ybcH ← PRK09936 family protein
RA 607,931 G→A 91.5% S7F (TCC→TTC)  fepA ← iron‑enterobactin outer membrane transporter
RA 625,364 G→T 74.7% G5G (GGG→GGT cstA → carbon starvation protein involved in peptide utilization; APC peptide transporter family protein
RA 630,088 G→T 23.9% A349A (GCG→GCT ybdL → methionine aminotransferase, PLP‑dependent
RA 632,013 T→G 7.3% K5Q (AAA→CAA)  ybdN ← PAPS reductase‑like domain protein
RA 632,406 T→A 11.9% K223N (AAA→AAT ybdO ← putative DNA‑binding transcriptional regulator
RA 639,231 G→A 93.6% L65F (CTT→TTT)  rnk ← regulator of nucleoside diphosphate kinase
RA 641,886 C→A 12.7% E51* (GAG→TAG)  citT ← citrate:succinate antiporter
RA 645,559 C→A 6.3% G130C (GGC→TGC)  citE ← citrate lyase, citryl‑ACP lyase (beta) subunit
RA 647,422 G→T 83.9% intergenic (‑110/‑269) citC ← / → citA [citrate [pro‑3S]‑lyase] ligase/sensory histidine kinase in two‑component regulatory system with CitB
RA 647,509 T→C 69.8% intergenic (‑197/‑182) citC ← / → citA [citrate [pro‑3S]‑lyase] ligase/sensory histidine kinase in two‑component regulatory system with CitB
RA 648,056 C→A 93.5% F122L (TTC→TTA citA → sensory histidine kinase in two‑component regulatory system with CitB
RA 652,865 Δ1 bp 88.6% coding (118/210 nt) cspE → constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein
RA 662,108 A→T 64.7% V522V (GTT→GTA mrdA ← transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)
RA 662,631 C→A 92.9% R348L (CGC→CTC)  mrdA ← transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)
RA 692,834 A→T 8.7% intergenic (‑245/+135) metT ← / ← asnB tRNA‑Met/asparagine synthetase B
RA 702,512 A→G 7.4% T322A (ACC→GCC)  glnS → glutamyl‑tRNA synthetase
RA 705,588 C→A 87.8% intergenic (+16/+68) chiQ → / ← fur chitosugar‑induced verified lipoprotein/ferric iron uptake regulon transcriptional repressor; autorepressor
RA 714,647 A→T 5.6% C424S (TGT→AGT)  speF ← ornithine decarboxylase isozyme, inducible
RA 724,813 A→T 69.3% intergenic (+17/‑226) ybfA → / → rhsC DUF2517 family protein/Rhs family putative polymorphic toxin
RA 733,809 G→T 6.4% E88* (GAG→TAG)  ybfD → H repeat‑associated putative transposase
RA 754,572 G→T 94.7% E137D (GAG→GAT sucA → 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding
RA 759,000 C→A 93.2% F177L (TTC→TTA sucC → succinyl‑CoA synthetase, beta subunit
RA 772,664 A→C 16.1% K289N (AAA→AAC tolA → membrane anchored protein in TolA‑TolQ‑TolR complex
RA 773,443 C→A 14.4% A83E (GCG→GAG)  tolB → periplasmic protein
RA 777,175 A→T 8.6% intergenic (+67/‑366) lysQ → / → nadA tRNA‑Lys/quinolinate synthase, subunit A
RA 788,902 G→T 92.9% R115S (CGT→AGT)  modF ← fused molybdate transporter subunits of ABC superfamily: ATP‑binding components
RA 795,472 T→A 13.8% E187V (GAG→GTG)  ybhD ← putative DNA‑binding transcriptional regulator
RA 800,488 G→T 35.4% V510V (GTG→GTT ybhJ → putative hydratase
RA 800,779 G→T 94.9% E607D (GAG→GAT ybhJ → putative hydratase
RA 802,887 G→T 5.6% intergenic (‑150/+2) ybhC ← / ← ybhB acyl‑CoA thioesterase, lipoprotein/kinase inhibitor homolog, UPF0098 family
RA 828,374 G→T 94.6% V11V (GTC→GTA ybiA ← DUF1768 family protein
RA 832,445 G→T 37.2% P213P (CCG→CCT ybiC → putative dehydrogenase
RA 833,505 G→T 11.9% intergenic (‑124/+141) ybiJ ← / ← ybiI DUF1471 family putative periplasmic protein/DksA‑type zinc finger protein
RA 843,299 C→A 23.0% W54C (TGG→TGT glnH ← glutamine transporter subunit
RA 876,447 G→A 94.7% D89N (GAT→AAT)  dacC → D‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 6a)
RA 879,085 G→T 91.7% intergenic (‑241/‑44) ybjG ← / → mdfA undecaprenyl pyrophosphate phosphatase/multidrug efflux system protein
RA 892,180 G→A 7.1% P197P (CCG→CCA potH → putrescine transporter subunit: membrane component of ABC superfamily
RA 902,091 A→T 10.4% L40Q (CTG→CAG)  ybjS ← putative NAD(P)H‑dependent oxidoreductase with NAD(P)‑binding Rossmann‑fold domain
RA 915,421 G→T 10.9% W244L (TGG→TTG)  macA → macrolide transporter subunit, membrane fusion protein (MFP) component
RA 917,071 G→T 6.8% V423V (GTG→GTT macB → fused macrolide transporter subunits of ABC superfamily: ATP‑binding component/membrane component
RA 923,339 G→T 14.4% S438* (TCG→TAG)  cydC ← fused glutathione, cysteine exporter subunits of ABC superfamily: membrane component/ATP‑binding component
RA 924,905 G→T 6.1% S505* (TCG→TAG)  cydD ← fused glutathione, cysteine exporter subunits of ABC superfamily: membrane component/ATP‑binding component
RA 925,160 G→T 32.8% S420* (TCA→TAA)  cydD ← fused glutathione, cysteine exporter subunits of ABC superfamily: membrane component/ATP‑binding component
RA 941,177 G→A 27.0% intergenic (‑164/‑150) ycaC ← / → ycaD putative isochorismatase family hydrolase/putative MFS‑type transporter
RA 943,573 G→T 27.1% A297S (GCC→TCC)  ycaM → putative transporter
RA 947,058 G→T 6.1% S651S (TCC→TCA pflB ← formate C‑acetyltransferase 1, anaerobic; pyruvate formate‑lyase 1
RA 950,103 T→C 8.9% intergenic (‑181/+225) focA ← / ← ycaO formate channel/ribosomal protein S12 methylthiotransferase accessory factor
RA 954,475 C→A 19.7% R70S (CGT→AGT)  aroA → 5‑enolpyruvylshikimate‑3‑phosphate synthetase
RA 960,043 G→T 15.7% G90C (GGC→TGC)  ycaI → ComEC family inner membrane protein
RA 968,415 G→T 94.7% S148* (TCG→TAG)  ycbC ← DUF218 superfamily protein
RA 975,610 G→T 7.0% V1277L (GTG→TTG)  mukB → chromosome condensin MukBEF, ATPase and DNA‑binding subunit
RA 1,047,553 G→A 5.3% E11K (GAA→AAA)  gnsA → putative phosphatidylethanolamine synthesis regulator
RA 1,048,928 C→A 32.1% E903* (GAA→TAA)  torS ← hybrid sensory histidine kinase in two‑component regulatory system with TorR
RA 1,049,407 G→T 38.6% T743K (ACG→AAG)  torS ← hybrid sensory histidine kinase in two‑component regulatory system with TorR
RA 1,055,901 A→T 15.0% Q397L (CAA→CTA)  torA → trimethylamine N‑oxide (TMAO) reductase I, catalytic subunit
RA 1,077,911 A→C 92.6% K71N (AAA→AAC efeO → inactive ferrous ion transporter EfeUOB
RA 1,084,103 A→T 24.7% L404* (TTA→TAA)  pgaB ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein
RA 1,098,065 C→A 5.6% K196N (AAG→AAT csgD ← csgBAC operon transcriptional regulator
RA 1,116,964 A→G 93.8% intergenic (‑21/+53) dinI ← / ← pyrC DNA damage‑inducible protein I/dihydro‑orotase
RA 1,118,506 A→T 6.0% L75Q (CTA→CAA)  yceB ← lipoprotein, DUF1439 family
RA 1,136,758 T→A 9.4% A1022A (GCA→GCT rne ← fused ribonucleaseE: endoribonuclease/RNA‑binding protein/RNA degradosome binding protein
RA 1,148,279 G→T 8.2% A295A (GCG→GCT fabF → 3‑oxoacyl‑[acyl‑carrier‑protein] synthase II
RA 1,151,750 G→T 90.3% R178L (CGC→CTC)  holB → DNA polymerase III, delta prime subunit
RA 1,152,278 G→T 90.6% E16* (GAA→TAA)  ycfH → putative DNase
RA 1,164,408 T→A 8.4% intergenic (‑120/‑121) ycfQ ← / → bhsA repressor for bhsA(ycfR)/biofilm, cell surface and signaling protein
RA 1,193,873 G→T 100% intergenic (‑180/‑278) ymfE ← / → lit e14 prophage; putative inner membrane protein/e14 prophage; cell death peptidase, inhibitor of T4 late gene expression
RA 1,211,169 C→A 36.5% intergenic (‑238/‑76) bluF ← / → ycgZ anti‑repressor for YcgE, blue light‑responsive; FAD‑binding; has c‑di‑GMP phosphodiesterase‑like EAL domain, but does not degrade c‑di‑GMP/RcsB connector protein for regulation of biofilm and acid‑resistance
RA 1,212,129 T→C 5.9% intergenic (+38/‑75) ariR → / → ymgC RcsB connector protein for regulation of biofilm and acid‑resistance/Blue light, low temperature and stress induced protein
RA 1,215,174 G→T 13.8% pseudogene (118/2648 nt) ycgH → pseudogene; putative ATP‑binding component of a transport system
RA 1,219,533 A→G 67.8% intergenic (+170/+202) ycgI → / ← minE pseudogene/cell division topological specificity factor
RA 1,220,996 G→T 90.3% R181R (CGG→AGG)  minC ← cell division inhibitor
RA 1,222,958 T→C 5.4% intergenic (‑30/‑212) pliG ← / → ycgL periplasmic inhibitor of g‑type lysozyme/UPF0745 family protein
RA 1,224,292 G→A 66.5% D8N (GAT→AAT)  ycgN → UPF0153 family cysteine cluster protein
RA 1,226,292 T→A 68.4% C24S (TGT→AGT)  umuD → DNA polymerase V, subunit D
RA 1,228,591 G→T 100% intergenic (‑105/+41) dsbB ← / ← nhaB oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I/sodium:proton antiporter
RA 1,266,181 C→G 5.0% intergenic (+265/+24) rdlC → / ← chaA sRNA antisense regulator affects LdrC translation; proposed addiction module in LDR‑C repeat, with toxic peptide LdrC/calcium/sodium:proton antiporter
RA 1,270,758 A→T 10.8% I176I (ATT→ATA narL ← response regulator in two‑component regulatory system with NarX (or NarQ)
RA 1,277,786 G→A 36.3% D823N (GAC→AAC)  narG → nitrate reductase 1, alpha subunit
RA 1,293,416 A→T 8.7% V54V (GTT→GTA adhE ← fused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase
RA 1,308,862 A→C 10.2% S196R (AGC→CGC)  ompW → outer membrane protein W
RA 1,309,158 G→A 12.6% I108I (ATC→ATT yciE ← putative rubrerythrin/ferritin‑like metal‑binding protein
RA 1,322,140 T→A 86.8% T159S (ACG→TCG)  btuR ← cob(I)yrinic acid a,c‑diamide adenosyltransferase
RA 1,337,533 G→T 33.8% A18E (GCA→GAA)  osmB ← lipoprotein
RA 1,345,484 A→C 9.0% intergenic (‑188/+180) fabI ← / ← ycjD enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein
RA 1,365,283 G→T 81.0% E271* (GAG→TAG)  ycjM → alpha amylase catalytic domain family protein
JC JC 1,376,043 IS5 (–) +4 bp 100% pseudogene (10‑13/126 nt) ycjV → pseudogene; putative ATP‑binding component of a transport system
RA 1,377,394 C→T 92.2% A275A (GCG→GCA ycjW ← LacI family putative transcriptional repressor
RA 1,386,008 G→T 79.9% Q35K (CAG→AAG)  ycjY ← S9 homolog non‑peptidase family protein
RA 1,388,406 G→T 89.3% R308L (CGC→CTC)  mppA → murein tripeptide (L‑ala‑gamma‑D‑glutamyl‑meso‑DAP) transporter subunit
RA 1,399,129 A→C 6.8% E44D (GAA→GAC abgR → putative DNA‑binding transcriptional regulator of abgABT operon
RA 1,403,313 C→T 24.5% intergenic (+23/‑73) ydaN → / → fnrS putative Zn(II) transporter/FNR‑activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq‑dependent; mediates negative FNR regulation
RA 1,424,234 C→A 36.0% S310* (TCA→TAA)  stfR → Rac prophage; putative tail fiber protein
JC JC 1,428,894 IS2 (–) +5 bp 29.4% intergenic (‑413/+317) ynaE ← / ← ttcC cold shock protein, Rac prophage/pseudogene, prophage Rac integration site ttcA duplication;Phage or Prophage Related
RA 1,439,518 C→A 25.8% S737S (TCC→TCA ydbH → putative membrane‑anchored protein, function unknown
RA 1,454,254 C→T 9.2% T315M (ACG→ATG)  paaH → 3‑hydroxyadipyl‑CoA dehydrogenase, NAD+‑dependent
RA 1,454,724 G→T 29.0% G472C (GGT→TGT)  paaH → 3‑hydroxyadipyl‑CoA dehydrogenase, NAD+‑dependent
RA 1,471,707 A→T 16.7% *769C (TGA→TGT ydbD → PF10971 family putative periplasmic methylglyoxal resistance protein
RA 1,472,138 G→T 20.1% A87A (GCG→GCT ynbA → inner membrane protein
RA 1,500,047 A→T 5.1% F97L (TTT→TTA ydcO ← BenE family inner membrane putative transporter
RA 1,501,734 G→T 26.0% D233Y (GAT→TAT)  ydcP → putative peptidase
RA 1,519,349 T→A 94.4% K297* (AAA→TAA)  ansP ← L‑asparagine transporter
RA 1,539,484 G→A 13.2% pseudogene (488/951 nt) yddK ← pseudogene, leucine‑rich protein; putative glycoportein
RA 1,548,144 G→T 91.9% intergenic (‑49/+85) adhP ← / ← maeA ethanol‑active dehydrogenase/acetaldehyde‑active reductase/malate dehydrogenase, (decarboxylating, NAD‑requiring) (malic enzyme)
RA 1,563,276 G→T 94.5% H491N (CAC→AAC)  gadC ← glutamate:gamma‑aminobutyric acid antiporter
RA 1,565,497 G→A 100% S269L (TCG→TTG)  gadB ← glutamate decarboxylase B, PLP‑dependent
RA 1,575,339 G→T 29.0% F481L (TTC→TTA ydeN ← putative Ser‑type periplasmic non‑aryl sulfatase
RA 1,603,440 G→A 29.2% intergenic (‑161/+46) yneE ← / ← uxaB bestrophin family putative inner membrane protein/altronate oxidoreductase, NAD‑dependent
RA 1,604,068 G→T 9.7% T290T (ACC→ACA uxaB ← altronate oxidoreductase, NAD‑dependent
RA 1,606,883 A→C 6.8% F209C (TTT→TGT)  glsB ← glutaminase 2
RA 1,620,961 T→G 32.4% Q226P (CAA→CCA)  dcp ← dipeptidyl carboxypeptidase II
RA 1,628,207 G→A 100% G110E (GGA→GAA)  pinQ → Qin prophage; putative site‑specific recombinase
RA 1,640,634 G→T 22.0% intergenic (+175/‑28) ydfV → / → flxA Qin prophage; uncharacterized protein/Qin prophage; uncharacterized protein
RA 1,649,456 C→T 12.1% intergenic (‑58/+148) rspA ← / ← ynfA bifunctional D‑altronate/D‑mannonate dehydratase/UPF0060 family inner membrane protein
RA 1,661,763 C→A 94.0% P353P (CCG→CCT mlc ← glucosamine anaerobic growth regulon transcriptional repressor; autorepressor
RA 1,662,484 G→A 6.5% S113L (TCG→TTG)  mlc ← glucosamine anaerobic growth regulon transcriptional repressor; autorepressor
RA 1,666,139 C→T 5.8% intergenic (+22/‑78) ydgU → / → ydgD stationary phase‑induced protein/putative peptidase
RA 1,696,612 A→T 73.2% E41D (GAA→GAT add → adenosine deaminase
RA 1,711,397 C→T 95.0% R28Q (CGA→CAA)  tyrS ← tyrosyl‑tRNA synthetase
RA 1,732,014 C→A 23.2% intergenic (‑212/‑87) ynhF ← / → purR stress response membrane/transcriptional repressor, hypoxanthine‑binding
RA 1,754,950 T→A 24.6% I367F (ATT→TTT)  sufD ← component of SufBCD Fe‑S cluster assembly scaffold
RA 1,770,281 T→A 89.4% D146E (GAT→GAA ydiF → putative acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit/beta subunit
RA 1,784,952 A→G 14.5% F184L (TTT→CTT)  ydiU ← UPF0061 family protein
RA 1,796,428 C→A 7.2% V134F (GTC→TTC)  thrS ← threonyl‑tRNA synthetase
RA 1,802,047 G→A 90.4% intergenic (+100/‑6) ydiZ → / → yniA uncharacterized protein/fructosamine kinase family protein
RA 1,824,100 T→A 5.2% D308V (GAT→GTT)  astA ← arginine succinyltransferase
RA 1,825,763 C→A 26.2% A159A (GCG→GCT astC ← succinylornithine transaminase, PLP‑dependent
RA 1,832,413 C→A 82.7% L315L (CTC→CTA ynjC → inner membrane putative ABC superfamily transporter permease
RA 1,833,730 C→A 100% R3S (CGT→AGT)  ynjE → molybdopterin synthase sulfurtransferase
RA 1,834,943 C→T 8.1% S407F (TCC→TTC)  ynjE → molybdopterin synthase sulfurtransferase
RA 1,861,773 T→A 18.3% I203I (ATT→ATA yeaG → protein kinase, endogenous substrate unidentified; autokinase
RA 1,865,422 C→A 91.4% R261R (CGA→AGA)  yeaI → putative membrane‑anchored diguanylate cyclase
RA 1,872,520 G→T 90.4% P183P (CCG→CCT yeaP → diguanylate cyclase
RA 1,882,569 C→T 9.5% G479S (GGC→AGC)  fadD ← acyl‑CoA synthetase (long‑chain‑fatty‑acid‑‑CoA ligase)
RA 1,884,212 T→C 5.1% E193G (GAA→GGA)  yeaY ← Slp family lipoprotein, RpoE‑regulated
RA 1,891,773 C→A 11.3% N195K (AAC→AAA sdaA → L‑serine dehydratase 1
RA 1,904,831 T→G 9.8% L100R (CTT→CGT)  yebQ → putative transporter
RA 1,906,096 T→A 6.5% K246N (AAA→AAT htpX ← putative endopeptidase
RA 1,907,821 T→G 5.7% Q418P (CAG→CCG)  prc ← carboxy‑terminal protease for penicillin‑binding protein 3
RA 1,909,342 T→A 94.9% E150D (GAA→GAT proQ ← RNA chaperone, putative ProP translation regulator
RA 1,915,555 A→C 5.4% Q359P (CAG→CCG)  rsmF → 16S rRNA m(5)C1407 methyltransferase, SAM‑dependent
RA 1,944,177 C→A 86.8% L201L (CTG→CTT aspS ← aspartyl‑tRNA synthetase
RA 1,948,689 G→A 24.2% intergenic (+19/+146) cmoB → / ← torZ tRNA (cmo5U34)‑carboxymethyltransferase, carboxy‑SAM‑dependent/trimethylamine N‑oxide reductase system III, catalytic subunit
RA 1,969,291 G→T 66.3% I97I (ATC→ATA cheA ← fused chemotactic sensory histidine kinase in two‑component regulatory system with CheB and CheY: sensory histidine kinase/signal sensing protein
RA 1,986,289 T→A 33.4% N4Y (AAT→TAT)  pgsA ← phosphatidylglycerophosphate synthetase
RA 1,997,314 G→T 23.8% intergenic (‑227/‑39) fliC ← / → fliD flagellar filament structural protein (flagellin)/flagellar filament capping protein
RA 1,999,632 G→T 24.9% intergenic (+73/‑5) fliT → / → amyA putative flagellar synthesis and assembly chaperone/cytoplasmic alpha‑amylase
RA 2,020,166 C→A 85.7% D445Y (GAC→TAC)  yedQ ← putative membrane‑anchored diguanylate cyclase
RA 2,035,090 C→A 21.9% Q79K (CAG→AAG)  zinT → zinc and cadmium binding protein, periplasmic
RA 2,035,236 A→T 16.6% K127N (AAA→AAT zinT → zinc and cadmium binding protein, periplasmic
RA 2,067,638 C→T 6.3% T873T (ACC→ACT flu → CP4‑44 prophage; antigen 43 (Ag43) phase‑variable biofilm formation autotransporter
RA 2,070,058 C→A 13.2% A90A (GCC→GCA yeeS → CP4‑44 prophage; putative DNA repair protein
RA 2,074,680 A→T 36.4% W22R (TGG→AGG)  sbmC ← DNA gyrase inhibitor
RA 2,078,521 C→A 14.1% V162V (GTG→GTT yeeE ← UPF0394 family inner membrane protein
RA 2,098,995 C→A 93.1% G182* (GGA→TGA)  wbbI ← d‑Galf:alpha‑d‑Glc beta‑1,6‑galactofuranosyltransferase
RA 2,100,755 C→T 5.4% V352V (GTG→GTA glf ← UDP‑galactopyranose mutase, FAD/NAD(P)‑binding
RA 2,108,219:1 +A 100% coding (1159/1395 nt) wcaM ← colanic acid biosynthesis protein
RA 2,114,911 A→T 86.5% L42Q (CTG→CAG)  wcaJ ← colanic biosynthesis UDP‑glucose lipid carrier transferase
RA 2,124,159 C→A 25.4% A67A (GCG→GCT wcaD ← putative colanic acid polymerase
RA 2,125,946 G→T 13.1% R31S (CGC→AGC)  wcaB ← putative acyl transferase
RA 2,136,421 A→G 12.1% D3D (GAT→GAC udk ← uridine/cytidine kinase
RA 2,137,996 T→G 8.8% L417R (CTA→CGA)  yegE → putative diguanylate cyclase
RA 2,139,910 G→T 100% R1055L (CGA→CTA)  yegE → putative diguanylate cyclase
RA 2,144,474 C→A 90.5% intergenic (‑8/‑192) yegI ← / → yegJ protein kinase‑related putative non‑specific DNA‑binding protein/uncharacterized protein
RA 2,149,603 A→T 21.7% E287V (GAA→GTA)  mdtB → multidrug efflux system, subunit B
RA 2,154,021 G→A 6.3% D719N (GAC→AAC)  mdtC → multidrug efflux system, subunit C
RA 2,168,487 A→T 19.2% D14E (GAT→GAA gatA ← galactitol‑specific enzyme IIA component of PTS
RA 2,168,795 C→A 70.5% D336Y (GAT→TAT)  gatZ ← D‑tagatose 1,6‑bisphosphate aldolase 2, subunit
RA 2,170,708 G→T 8.2% intergenic (‑25/+283) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
RA 2,170,713 C→A 27.9% intergenic (‑30/+278) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
RA 2,170,724 G→T 25.4% intergenic (‑41/+267) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
RA 2,170,749 G→T 8.9% intergenic (‑66/+242) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
RA 2,179,205 G→T 65.5% A24E (GCG→GAG)  rcnR ← transcriptional repressor of rcnA
RA 2,181,645 A→G 32.6% N83N (AAT→AAC yehA ← putative fimbrial‑like adhesin protein
RA 2,185,860 G→A 22.1% intergenic (‑159/+134) yehD ← / ← yehE putative fimbrial‑like adhesin protein/DUF2574 family protein
RA 2,194,695 T→A 66.7% L313Q (CTA→CAA)  yehI → DUF4132 domain‑containing protein
RA 2,195,449 A→T 92.9% E564D (GAA→GAT yehI → DUF4132 domain‑containing protein
RA 2,206,521 C→A 14.8% D535Y (GAT→TAT)  yehU ← inner membrane putative sensory kinase in two‑component system with YehT
RA 2,216,296 C→A 86.9% I211I (ATC→ATA dld → D‑lactate dehydrogenase, FAD‑binding, NADH independent
RA 2,225,190 C→T 27.9% G231G (GGC→GGT yohK → LrgB family inner membrane protein
RA 2,237,098 G→T 90.2% H12N (CAT→AAT)  folE ← GTP cyclohydrolase I
RA 2,237,441 G→T 7.1% R18L (CGA→CTA)  yeiG → S‑formylglutathione hydrolase
RA 2,254,517 C→T 14.2% A125T (GCC→ACC)  fruA ← fused fructose‑specific PTS enzymes: IIBcomponent/IIC components
RA 2,254,766 C→A 5.4% E42* (GAA→TAA)  fruA ← fused fructose‑specific PTS enzymes: IIBcomponent/IIC components
RA 2,257,823 C→A 8.9% P161Q (CCG→CAG)  setB → lactose/glucose efflux system
RA 2,264,143 G→A 79.6% intergenic (+119/‑62) mepS → / → rtn murein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein/resistance protein for phages lambda and N4, putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
RA 2,273,246 C→T 5.3% intergenic (‑7/+21) bcr ← / ← rsuA bicyclomycin/cysteine/sulfonamide efflux transporter/16S rRNA pseudouridine(516) synthase
RA 2,289,374 C→A 89.7% V142V (GTG→GTT ccmC ← heme exporter subunit
RA 2,298,128 C→T 11.1% intergenic (+256/+459) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,298,492 A→G 6.8% intergenic (+620/+95) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,303,070 T→G 71.5% Q272P (CAG→CCG)  ada ← fused DNA‑binding transcriptional dual regulator/O6‑methylguanine‑DNA methyltransferase
RA 2,305,116 C→A 91.4% intergenic (‑103/+9) apbE ← / ← ompC putative thiamine‑synthetic flavin transferase lipoprotein/outer membrane porin protein C
RA 2,305,135 T→C 69.5% Y365C (TAC→TGC)  ompC ← outer membrane porin protein C
RA 2,311,308 T→A 5.8% Q683L (CAG→CTG)  rcsC ← hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
RA 2,330,502 C→A 27.1% D800Y (GAT→TAT)  gyrA ← DNA gyrase (type II topoisomerase), subunit A
RA 2,352,573 C→A 93.7% P353P (CCG→CCT rhmT ← putative L‑rhamnonate transporter
RA 2,352,965 C→A 16.7% E223* (GAA→TAA)  rhmT ← putative L‑rhamnonate transporter
RA 2,359,673 G→T 93.2% M89I (ATG→ATT arnB → uridine 5'‑(beta‑1‑threo‑pentapyranosyl‑4‑ulose diphosphate) aminotransferase, PLP‑dependent
RA 2,361,054 C→A 5.6% H169N (CAT→AAT)  arnC → undecaprenyl phosphate‑L‑Ara4FN transferase
RA 2,362,338 T→A 100% L274Q (CTG→CAG)  arnA → fused UDP‑L‑Ara4N formyltransferase/UDP‑GlcA C‑4'‑decarboxylase
RA 2,370,361:1 +G 93.0% coding (711/759 nt) menH ← 2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase
RA 2,370,987 C→A 92.3% E29* (GAA→TAA)  menH ← 2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase
RA 2,379,310 C→A 17.4% intergenic (‑109/‑29) yfbK ← / → yfbL Von Willebrand factor domain putative lipoprotein/putative peptidase
RA 2,381,180 G→A 18.0% intergenic (+264/+9) yfbM → / ← yfbN DUF1877 family protein/uncharacterized protein
RA 2,399,210 G→T 71.2% S304* (TCA→TAA)  lrhA ← transcriptional repressor of flagellar, motility and chemotaxis genes
RA 2,405,946 T→G 100% Q43P (CAG→CCG)  yfbU ← UPF0304 family protein
RA 2,416,101 C→A 66.8% intergenic (+21/+27) yfcH → / ← yfcI putative NAD‑dependent nucleotide‑sugar epimerase/transposase_31 family protein
RA 2,421,405 C→T 5.6% intergenic (‑135/+131) argT ← / ← ubiX lysine/arginine/ornithine transporter subunit/3‑octaprenyl‑4‑hydroxybenzoate carboxy‑lyase
RA 2,422,841 C→A 88.6% D293Y (GAT→TAT)  purF ← amidophosphoribosyltransferase
RA 2,424,074 C→T 94.1% D57N (GAC→AAC)  cvpA ← colicin V production membrane protein
RA 2,436,760 G→T 11.7% T506T (ACG→ACT mnmC → fused 5‑methylaminomethyl‑2‑thiouridine‑forming enzyme methyltransferase and FAD‑dependent demodification enzyme
RA 2,450,335 C→T 93.2% intergenic (‑44/+159) sixA ← / ← fadJ phosphohistidine phosphatase/fused enoyl‑CoA hydratase and epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase
RA 2,450,346 G→T 65.8% intergenic (‑55/+148) sixA ← / ← fadJ phosphohistidine phosphatase/fused enoyl‑CoA hydratase and epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase
RA 2,452,454 G→T 69.0% P62Q (CCG→CAG)  fadJ ← fused enoyl‑CoA hydratase and epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase
RA 2,454,297 T→G 87.7% E39D (GAA→GAC yfcZ ← UPF0381 family protein
RA 2,457,708 G→T 22.0% intergenic (+159/+23) yfdF → / ← mlaA uncharacterized protein/ABC transporter maintaining OM lipid asymmetry, OM lipoprotein component
RA 2,463,713 T→C 8.7% I368I (ATT→ATC gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
RA 2,463,915 C→A 13.2% Q436K (CAA→AAA)  gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
RA 2,474,418 G→T 93.4% S413* (TCA→TAA)  emrY ← putative multidrug efflux system
RA 2,482,981 C→A 10.0% E229* (GAA→TAA)  yfdV ← putative transporter
RA 2,493,283 C→A 18.1% N378K (AAC→AAA ypdA → sensor kinase regulating yhjX; pyruvate‑responsive YpdAB two‑component system
RA 2,520,652 C→A 70.4% I116I (ATC→ATA yfeH → putative inorganic ion transporter
RA 2,525,538 G→T 21.7% A239A (GCG→GCT cysZ → EI24 family inner membrane protein; putative sulfate transporter
RA 2,526,745 G→A 6.8% intergenic (+6/‑378) cysK → / → ptsH cysteine synthase A, O‑acetylserine sulfhydrolase A subunit/phosphohistidinoprotein‑hexose phosphotransferase component of PTS system (Hpr)
RA 2,544,288 C→A 8.9% V96V (GTG→GTT yfeY ← RpoE‑regulated lipoprotein
RA 2,549,676 C→A 65.3% L28L (CTG→CTT eutL ← putative ethanol utilization carboxysome structural protein
RA 2,568,720 C→T 67.5% A33T (GCC→ACC)  eutP ← putative P‑loop NTPase ethanolamine utilization protein
RA 2,576,325 C→A 92.1% E171D (GAG→GAT nudK ← GDP‑mannose pyrophosphatase
RA 2,587,029:1 +G 89.7% coding (101/699 nt) ypfH ← palmitoyl‑CoA esterase activity, uncertain physiological substrate
RA 2,587,434 T→A 5.5% L595F (TTA→TTT tmcA ← elongator methionine tRNA (ac4C34) acetyltransferase
RA 2,589,933 G→A 74.9% T55I (ACC→ATC)  ypfJ ← putative neutral zinc metallopeptidase
RA 2,590,777 C→T 8.7% P67P (CCG→CCA purC ← phosphoribosylaminoimidazole‑succinocarboxamide synthetase
RA 2,595,292 C→A 7.2% S39* (TCG→TAG)  hyfB → hydrogenase 4, membrane subunit
RA 2,596,499 G→A 6.0% G441G (GGG→GGA hyfB → hydrogenase 4, membrane subunit
RA 2,602,418 C→A 19.1% I191I (ATC→ATA hyfG → hydrogenase 4, subunit
RA 2,605,882 C→A 8.3% S208R (AGC→AGA hyfR → hydrogenase‑4 transcriptional activator
RA 2,606,516 G→T 92.4% D420Y (GAC→TAC)  hyfR → hydrogenase‑4 transcriptional activator
RA 2,609,187 C→A 9.6% A18A (GCG→GCT yfgO ← putative UPF0118 family inner membrane permease
RA 2,610,919 C→A 65.2% intergenic (+3/‑18) bepA → / → yfgD periplasmic metalloprotease and chaperone for OM protein maintenance and assembly/putative oxidoreductase
RA 2,614,168 C→A 91.7% D22Y (GAT→TAT)  upp ← uracil phosphoribosyltransferase
RA 2,651,665 G→T 100% G210G (GGC→GGA hscA ← DnaK‑like molecular chaperone specific for IscU
RA 2,664,779 G→T 5.5% D66Y (GAT→TAT)  hcaB → 2,3‑dihydroxy‑2,3‑dihydrophenylpropionate dehydrogenase
RA 2,671,274 C→A 94.3% T149T (ACG→ACT yphE ← putative sugar transporter subunit of ABC superfamily, ATP‑binding component
RA 2,674,941 G→T 14.0% I388I (ATC→ATA yphG ← DUF4380 domain‑containing TPR repeat protein
RA 2,681,873 T→A 93.1% Q97L (CAG→CTG)  glrR ← response regulator regulating glmY sRNA in two‑component system with sensor protein GlrK
RA 2,683,829 C→A 64.3% R210L (CGT→CTT)  glrK ← sensor protein kinase regulating glmY sRNA in two‑component system with response regulator GlrR
RA 2,689,722 T→G 20.1% L188* (TTA→TGA)  mltF → membrane‑bound lytic transglycosylase F, murein hydrolase
RA 2,707,492 A→C 8.1% K413T (AAG→ACG)  srmB → ATP‑dependent RNA helicase
RA 2,711,673 A→C 6.0% V72G (GTT→GGT)  yfiF ← putative methyltransferase
RA 2,715,643 G→T 92.4% E778* (GAA→TAA)  pka → protein lysine acetyltransferase
RA 2,718,953 G→T 13.3% I51I (ATC→ATA kgtP ← alpha‑ketoglutarate transporter
RA 2,719,426 T→C 100% intergenic (‑321/+2) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
RA 2,726,786 C→A 66.6% A249A (GCG→GCT clpB ← protein disaggregation chaperone
RA 2,730,313 G→A 21.6% intergenic (+71/‑200) bamD → / → raiA BamABCDE complex OM biogenesis lipoprotein/cold shock protein associated with 30S ribosomal subunit
RA 2,733,753 C→T 100% E253K (GAG→AAG)  aroF ← 3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tyrosine‑repressible
RA 2,771,358 G→A 85.3% intergenic (+217/+147) ypjF → / ← ypjA CP4‑57 prophage; toxin of the YpjF‑YfjZ toxin‑antitoxin system/adhesin‑like autotransporter
RA 2,772,026 G→A 6.2% L1354L (CTG→TTG)  ypjA ← adhesin‑like autotransporter
RA 2,775,830 C→T 18.4% D86N (GAT→AAT)  ypjA ← adhesin‑like autotransporter
RA 2,779,067 C→A 70.2% intergenic (‑697/+54) ypjC ← / ← ileY pseudogene/tRNA‑Ile
RA 2,781,864 C→T 5.3% pseudogene (2109/2253 nt) ygaQ → uncharacterized protein; putative enzyme
RA 2,785,895 G→A 6.9% D422N (GAT→AAT)  gabD → succinate‑semialdehyde dehydrogenase I, NADP‑dependent
RA 2,809,621 C→A 23.8% D60Y (GAT→TAT)  gshA ← glutamate‑cysteine ligase
RA 2,811,408 T→A 5.0% intergenic (‑189/+10) argQ ← / ← argZ tRNA‑Arg/tRNA‑Arg
RA 2,819,086 A→G 7.3% intergenic (‑151/‑105) mltB ← / → srlA membrane‑bound lytic murein transglycosylase B/glucitol/sorbitol‑specific enzyme IIC component of PTS
RA 2,825,783 C→T 100% intergenic (‑135/‑52) norR ← / → norV anaerobic nitric oxide reductase DNA‑binding transcriptional activator/anaerobic nitric oxide reductase flavorubredoxin
RA 2,828,952 C→A 74.4% T611T (ACG→ACT hypF ← carbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases
RA 2,841,044 C→A 48.5% A519A (GCG→GCT hycC ← hydrogenase 3, membrane subunit
RA 2,850,790 C→A 91.8% I113I (ATC→ATA mutS → methyl‑directed mismatch repair protein
RA 2,853,682 G→A 6.2% M188I (ATG→ATA pphB → serine/threonine‑specific protein phosphatase 2
RA 2,853,749 C→T 7.6% R211W (CGG→TGG)  pphB → serine/threonine‑specific protein phosphatase 2
RA 2,869,269 C→A 6.5% E140D (GAG→GAT cysD ← sulfate adenylyltransferase, subunit 2
RA 2,873,087 G→T 93.5% V15V (GTC→GTA ygbT ← multifunctional endonuclease Cas1, CRISPR adaptation protein; DNA repair enzyme
RA 2,876,760 C→T 93.9% G246G (GGG→GGA casA ← CRISP RNA (crRNA) containing Cascade antiviral complex protein
RA 2,878,668 G→T 82.9% V637V (GTC→GTA ygcB ← R‑loop helicase‑annealase Cas3 needed for Cascade anti‑viral activity
RA 2,879,449 G→T 27.2% S377* (TCA→TAA)  ygcB ← R‑loop helicase‑annealase Cas3 needed for Cascade anti‑viral activity
RA 2,888,584 G→T 68.5% I185I (ATC→ATA ygcQ ← putative flavoprotein
RA 2,897,136 C→A 7.3% intergenic (+403/+970) ygcE → / ← queE putative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
RA 2,901,394 C→A 20.2% A544A (GCG→GCT pyrG ← CTP synthetase
RA 2,928,446 G→T 12.3% A285S (GCT→TCT)  fucP → L‑fucose transporter
RA 2,931,717 C→A 93.9% N307K (AAC→AAA fucK → L‑fuculokinase
RA 2,934,843 G→T 100% H50N (CAT→AAT)  ygdD ← UPF0382 family inner membrane protein
RA 2,940,010 G→A 6.8% N176N (AAC→AAT mltA ← membrane‑bound lytic murein transglycosylase A
RA 2,944,129 C→T 7.8% E564E (GAG→GAA recD ← exonuclease V (RecBCD complex), alpha chain
RA 2,953,192 C→A 15.9% D866Y (GAC→TAC)  recC ← exonuclease V (RecBCD complex), gamma chain
RA 2,959,004 G→A 68.3% I131I (ATC→ATT lgt ← phosphatidylglycerol‑prolipoprotein diacylglyceryl transferase
RA 2,983,868 G→T 12.0% intergenic (‑149/‑45) yqeK ← / → ygeF uncharacterized protein/pseudogene
RA 2,988,774 C→A 12.6% pseudogene (346/447 nt) ygeN ← pseudogene
RA 2,989,295 C→A 91.0% pseudogene (85/261 nt) ygeN ← pseudogene
RA 2,993,211 C→A 92.1% V14L (GTG→TTG)  ygeR ← LysM domain‑containing M23 family putative peptidase; septation lipoprotein
RA 2,993,699 C→A 57.9% intergenic (‑449/‑5) ygeR ← / → xdhA LysM domain‑containing M23 family putative peptidase; septation lipoprotein/xanthine dehydrogenase, molybdenum binding subunit
RA 2,998,350 C→A 9.0% E266* (GAA→TAA)  ygeV ← putative sigma‑54‑interacting transcriptional activator
RA 3,001,919 C→A 14.4% H351N (CAT→AAT)  ygeX → 2,3‑diaminopropionate ammonia lyase, PLP‑dependent
RA 3,009,360 C→A 6.2% intergenic (+263/‑59) mocA → / → ygfK CTP:molybdopterin cytidylyltransferase/putative Fe‑S subunit oxidoreductase subunit
RA 3,017,285 G→T 63.7% D871Y (GAC→TAC)  xdhD → putative hypoxanthine oxidase, molybdopterin‑binding/Fe‑S binding
RA 3,020,489 G→T 13.6% D4Y (GAC→TAC)  ygfQ → putative purine permease
RA 3,020,629 C→A 11.1% I50I (ATC→ATA ygfQ → putative purine permease
RA 3,024,983 C→G 84.3% G86G (GGC→GGG uacT → uric acid permease
RA 3,037,190 C→T 6.5% P57L (CCA→CTA)  bglA → 6‑phospho‑beta‑glucosidase A
RA 3,039,409 T→G 14.7% intergenic (‑149/+118) ygfF ← / ← gcvP putative NAD(P)‑dependent oxidoreductase with NAD(P)‑binding Rossmann‑fold domain/glycine decarboxylase, PLP‑dependent, subunit (protein P) of glycine cleavage complex
RA 3,044,366 C→A 5.8% intergenic (‑340/+108) gcvT ← / ← ubiI aminomethyltransferase, tetrahydrofolate‑dependent, subunit (T protein) of glycine cleavage complex/2‑octaprenylphenol hydroxylase, FAD‑dependent
RA 3,044,464 G→A 94.6% intergenic (‑438/+10) gcvT ← / ← ubiI aminomethyltransferase, tetrahydrofolate‑dependent, subunit (T protein) of glycine cleavage complex/2‑octaprenylphenol hydroxylase, FAD‑dependent
RA 3,050,591 C→A 17.5% A393A (GCG→GCT serA ← D‑3‑phosphoglycerate dehydrogenase
RA 3,055,546 C→A 25.6% I446I (ATC→ATA scpA → methylmalonyl‑CoA mutase
RA 3,059,429 G→A 10.6% V423V (GTG→GTA scpC → propionyl‑CoA:succinate CoA transferase
RA 3,064,914 G→T 22.5% I356I (ATC→ATA pgk ← phosphoglycerate kinase
RA 3,075,114 C→A 91.3% intergenic (‑120/‑158) tktA ← / → loiP transketolase 1, thiamine triphosphate‑binding/Phe‑Phe periplasmic metalloprotease, OM lipoprotein; low salt‑inducible; heat shock protein that binds Era
RA 3,076,526 C→A 92.8% D211Y (GAC→TAC)  speB ← agmatinase
RA 3,080,618 T→A 94.2% V185E (GTG→GAG)  metK → S‑adenosylmethionine synthetase
RA 3,084,266 C→A 7.3% L187L (CTC→CTA endA → DNA‑specific endonuclease I
RA 3,090,144 C→A 8.9% G35G (GGC→GGA rdgB → dITP/XTP pyrophosphatase
RA 3,110,751 C→A 73.0% R574L (CGT→CTT)  yghJ ← DUF4092 family putative lipoprotein peptidase
RA 3,111,434 C→A 91.1% A346A (GCG→GCT yghJ ← DUF4092 family putative lipoprotein peptidase
RA 3,120,216 C→A 91.3% D389Y (GAT→TAT)  glcD ← glycolate oxidase subunit, FAD‑linked
RA 3,127,390 G→A 6.3% intergenic (‑74/‑100) yghS ← / → yghT putative ATP‑binding protein/putative ATP‑binding protein
RA 3,144,158 C→A 39.2% intergenic (+253/+19) yghA → / ← exbD putative oxidoreductase/membrane spanning protein in TonB‑ExbB‑ExbD complex
RA 3,152,613 C→A 9.0% P631P (CCG→CCT ygiQ ← Radical SAM superfamily protein
RA 3,173,669 G→T 89.0% A189A (GCG→GCT ygiB → DUF1190 family protein
RA 3,181,577 G→T 5.9% pseudogene (798/2445 nt) yqiG → pseudogene; fimbrial export usher family;putative membrane; Not classified; putative membrane protein
RA 3,185,542 C→T 7.4% intergenic (‑244/‑25) glgS ← / → yqiJ motility and biofilm regulator/DUF1449 family inner membrane protein
RA 3,202,506 C→A 7.5% S375* (TCG→TAG)  ttdT → L‑tartrate/succinate antiporte
RA 3,204,755 T→A 9.9% V97E (GTG→GAG)  dnaG → DNA primase
RA 3,224,927 C→A 66.1% intergenic (+329/‑97) ygjK → / → fadH alpha‑glucosidase/2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked
RA 3,225,516 G→T 18.0% E165* (GAA→TAA)  fadH → 2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked
RA 3,243,810 C→T 15.4% intergenic (+75/‑111) yqjK → / → yqjF uncharacterized protein/putative quinol oxidase subunit
RA 3,245,848 G→T 59.7% L62L (CTG→CTT yhaH → DUF805 family inner membrane protein
RA 3,247,010 C→A 12.6% P188P (CCG→CCT yhaJ ← LysR family putative transcriptional regulator
RA 3,252,258 C→A 92.5% D251Y (GAT→TAT)  tdcG ← L‑serine dehydratase 3, anaerobic
RA 3,252,867 C→A 6.4% D48Y (GAT→TAT)  tdcG ← L‑serine dehydratase 3, anaerobic
RA 3,260,182 C→A 11.4% M81I (ATG→ATT tdcA ← tdc operon transcriptional activator
RA 3,262,844 T→A 13.4% S357S (TCT→TCA yhaC → pentapetide repeats‑related protein
RA 3,263,502 C→T 5.1% intergenic (+541/+73) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component
RA 3,266,646 C→A 36.1% G91C (GGT→TGT)  garL ← alpha‑dehydro‑beta‑deoxy‑D‑glucarate aldolase
RA 3,272,550 G→T 6.7% G93V (GGG→GTG)  kbaZ → tagatose 6‑phosphate aldolase 1, kbaZ subunit
RA 3,278,427 C→A 18.3% C128* (TGC→TGA agaC → N‑acetylgalactosamine‑specific enzyme IIC component of PTS
RA 3,301,310 C→T 5.8% intergenic (‑18/+89) yrbN ← / ← nlpI uncharacterized protein/lipoprotein involved in osmotic sensitivity and filamentation
RA 3,302,027 G→T 88.8% S86* (TCG→TAG)  nlpI ← lipoprotein involved in osmotic sensitivity and filamentation
RA 3,308,823 T→C 8.0% K184R (AAG→AGG)  infB ← translation initiation factor IF‑2
RA 3,311,918 C→T 19.1% intergenic (‑270/‑78) metY ← / → argG tRNA‑Met/argininosuccinate synthetase
RA 3,314,870 C→A 44.2% A35S (GCG→TCG)  yhbX ← putative EptAB family phosphoethanolamine transferase, inner membrane protein
RA 3,316,300 C→A 5.9% R377L (CGT→CTT)  glmM ← phosphoglucosamine mutase
RA 3,335,071 A→T 13.0% V149V (GTA→GTT kdsD → D‑arabinose 5‑phosphate isomerase
RA 3,345,202 C→A 8.5% D395Y (GAC→TAC)  arcB ← aerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB
RA 3,347,778 G→A 61.6% intergenic (‑369/‑306) yhcC ← / → gltB putative Fe‑S oxidoreductase, Radical SAM superfamily protein/glutamate synthase, large subunit
RA 3,354,405 C→T 92.7% intergenic (+430/‑130) gltD → / → gltF glutamate synthase, 4Fe‑4S protein, small subunit/periplasmic protein
RA 3,357,615 A→C 12.8% N484H (AAT→CAT)  yhcD → putative outer membrane fimbrial subunit usher protein
RA 3,365,607 G→T 69.4% I109I (ATC→ATA nanT ← sialic acid transporter
RA 3,371,372 G→T 11.3% I65I (ATC→ATA rpsI ← 30S ribosomal subunit protein S9
RA 3,373,985 C→A 93.3% intergenic (+37/‑117) yhcB → / → degQ DUF1043 family inner membrane‑anchored protein/serine endoprotease, periplasmic
RA 3,375,260 G→T 93.8% D387Y (GAT→TAT)  degQ → serine endoprotease, periplasmic
RA 3,380,336 C→A 15.1% A404A (GCG→GCT aaeB ← p‑hydroxybenzoic acid efflux system component
RA 3,388,112 G→T 8.8% I502I (ATC→ATA yhdP ← DUF3971‑AsmA2 domains protein
RA 3,396,082 G→T 87.4% R204S (CGT→AGT)  csrD ← targeting factor for csrBC sRNA degradation
RA 3,405,662 C→A 10.4% I217I (ATC→ATA yhdJ → DNA adenine methyltransferase, SAM‑dependent
RA 3,409,129 C→A 8.4% F246L (TTC→TTA acrF → multidrug efflux system protein
RA 3,409,403 C→A 11.8% H338N (CAC→AAC)  acrF → multidrug efflux system protein
RA 3,411,456 C→A 27.9% P1022Q (CCG→CAG)  acrF → multidrug efflux system protein
RA 3,422,390 C→A 8.9% intergenic (‑269/‑205) rrsD ← / → yrdA 16S ribosomal RNA of rrnD operon/bacterial transferase hexapeptide domain protein
RA 3,433,260 G→T 7.0% I33I (ATC→ATA rplQ ← 50S ribosomal subunit protein L17
RA 3,440,151 G→T 89.5% I31I (ATC→ATA rpsN ← 30S ribosomal subunit protein S14
RA 3,440,444 G→T 91.7% S118S (TCC→TCA rplE ← 50S ribosomal subunit protein L5
RA 3,440,832 T→C 35.5% N99D (AAC→GAC)  rplX ← 50S ribosomal subunit protein L24
RA 3,449,569 C→A 11.3% I211I (ATC→ATA gspC → general secretory pathway component, cryptic
RA 3,455,111 C→A 11.0% A95A (GCC→GCA gspH → putative general secretory pathway component, cryptic
RA 3,455,867 C→A 94.6% I55I (ATC→ATA gspJ → putative general secretory pathway component, cryptic
RA 3,466,043 C→A 6.7% A277A (GCG→GCT fusA ← protein chain elongation factor EF‑G, GTP‑binding
RA 3,473,139 G→T 93.5% I276I (ATC→ATA kefB ← potassium:proton antiporter
RA 3,479,542 C→A 8.3% H22N (CAT→AAT)  crp → cAMP‑activated global transcription factor, mediator of catabolite repression
RA 3,479,769 A→T 70.7% E97D (GAA→GAT crp → cAMP‑activated global transcription factor, mediator of catabolite repression
RA 3,488,966 G→C 32.7% A533P (GCC→CCC)  nirB → nitrite reductase, large subunit, NAD(P)H‑binding
RA 3,489,397 C→A 30.2% F676L (TTC→TTA nirB → nitrite reductase, large subunit, NAD(P)H‑binding
RA 3,498,206 C→T 6.7% intergenic (+64/+88) frlR → / ← yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
RA 3,498,214 T→G 12.1% intergenic (+72/+80) frlR → / ← yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
RA 3,498,239 A→C 10.3% intergenic (+97/+55) frlR → / ← yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
RA 3,501,571 C→A 32.4% G127C (GGC→TGC)  php ← phosphotriesterase homology protein
RA 3,507,749 G→T 30.2% H224N (CAT→AAT)  rpe ← D‑ribulose‑5‑phosphate 3‑epimerase
RA 3,519,657 G→T 100% intergenic (‑149/‑171) nudE ← / → yrfF adenosine nucleotide hydrolase; substrates include Ap3A, Ap2A, ADP‑ribose, NADH/inner membrane protein
RA 3,520,789 G→T 17.1% R321L (CGA→CTA)  yrfF → inner membrane protein
RA 3,527,739 C→A 6.8% F521L (TTC→TTA pck → phosphoenolpyruvate carboxykinase
RA 3,543,959 G→T 90.4% A625E (GCG→GAG)  malP ← maltodextrin phosphorylase
RA 3,561,574 C→A 94.0% W372L (TGG→TTG)  glgC ← glucose‑1‑phosphate adenylyltransferase
RA 3,595,522 C→A 24.1% D196Y (GAC→TAC)  ftsE ← putative ABC superfamily transporter ATP‑binding subunit
RA 3,622,519 C→A 14.8% R127L (CGA→CTA)  rbbA ← ribosome‑associated ATPase: ATP‑binding protein/ATP‑binding membrane protein
RA 3,632,965 A→G 27.5% L39S (TTG→TCG)  uspB ← universal stress (ethanol tolerance) protein B
RA 3,662,660 T→C 33.4% intergenic (‑112/‑292) yhjA ← / → treF putative cytochrome C peroxidase/cytoplasmic trehalase
RA 3,668,380 A→C 6.9% I79L (ATT→CTT)  yhjE → MFS superfamily sugar transport 1 family protein
RA 3,679,301 T→G 89.9% Q1078P (CAG→CCG)  bcsC ← cellulose synthase subunit
RA 3,679,324 C→A 94.7% M1070I (ATG→ATT bcsC ← cellulose synthase subunit
RA 3,680,910 G→T 33.0% H542N (CAT→AAT)  bcsC ← cellulose synthase subunit
RA 3,681,219 C→A 11.8% A439S (GCG→TCG)  bcsC ← cellulose synthase subunit
RA 3,717,788 C→A 71.5% P297P (CCG→CCT glyQ ← glycine tRNA synthetase, alpha subunit
RA 3,721,485 T→G 9.5% Y416S (TAC→TCC)  xylB ← xylulokinase
RA 3,738,431 C→A 91.8% P90T (CCA→ACA)  yiaN → 2,3‑diketo‑L‑gulonate TRAP transporter large permease protein
RA 3,744,506 C→A 27.6% G241G (GGG→GGT yiaT ← putative outer membrane protein
RA 3,762,852 C→A 8.9% pseudogene (148/327 nt) yibV → pseudogene
RA 3,774,382 T→G 16.6% intergenic (+5/‑193) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
RA 3,780,155 C→A 6.7% I512I (ATC→ATA gpmM → phosphoglycero mutase III, cofactor‑independent
RA 3,785,915 G→T 93.6% intergenic (‑4/+271) kbl ← / ← yibB glycine C‑acetyltransferase/YibB family protein, function unknown
RA 3,808,726 C→A 5.5% E135* (GAG→TAG)  pyrE ← orotate phosphoribosyltransferase
RA 3,810,195 C→A 92.6% R54S (CGT→AGT)  yicC → UPF0701 family protein
RA 3,811,734 T→G 12.0% A205A (GCT→GCG dinD → DNA damage‑inducible protein
RA 3,816,601 A→T 100% H281L (CAC→CTC)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,818,010 C→A 93.1% H46N (CAT→AAT)  trmH → tRNA mG18‑2'‑O‑methyltransferase, SAM‑dependent
RA 3,822,340 A→C 13.2% Q12H (CAA→CAC xanP → xanthine permease
RA 3,825,702 C→A 91.9% K732N (AAG→AAT yicI ← putative alpha‑glucosidase
RA 3,826,705 T→A 8.1% Y398F (TAC→TTC)  yicI ← putative alpha‑glucosidase
RA 3,827,647 A→C 5.8% L84R (CTC→CGC)  yicI ← putative alpha‑glucosidase
RA 3,830,197 C→A 70.1% intergenic (+281/‑116) yicT → / → setC pseudogene/putative arabinose efflux transporter
RA 3,830,311 G→T 83.4% intergenic (+395/‑2) yicT → / → setC pseudogene/putative arabinose efflux transporter
RA 3,837,177 C→T 13.5% intergenic (‑28/‑147) yicO ← / → ade putative adenine permease/cryptic adenine deaminase
RA 3,844,801 C→A 5.2% M448I (ATG→ATT ilvB ← acetolactate synthase 2 large subunit
RA 3,849,854 A→T 25.3% H27L (CAC→CTC)  yidI → inner membrane protein
RA 3,850,839 G→T 18.0% P309Q (CCG→CAG)  yidJ ← sulfatase/phosphatase superfamily protein
RA 3,860,159 C→A 93.7% P33P (CCG→CCT ibpB ← heat shock chaperone
RA 3,888,527 G→T 10.9% L172L (CTG→CTT chrR → chromate reductase, Class I, flavoprotein
RA 3,904,487 G→T 23.4% I133I (ATC→ATA pstS ← periplasmic phosphate binding protein, high‑affinity
RA 3,907,094 G→A 11.5% intergenic (‑66/+96) glmS ← / ← glmU L‑glutamine:D‑fructose‑6‑phosphate aminotransferase/fused N‑acetyl glucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyl transferase
RA 3,907,559 A→T 20.0% P334P (CCT→CCA glmU ← fused N‑acetyl glucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyl transferase
RA 3,910,894 C→A 88.3% M244I (ATG→ATT atpG ← F1 sector of membrane‑bound ATP synthase, gamma subunit
RA 3,911,608 C→A 6.1% E6D (GAG→GAT atpG ← F1 sector of membrane‑bound ATP synthase, gamma subunit
RA 3,916,830 C→A 66.8% D71Y (GAT→TAT)  rsmG ← 16S rRNA m(7)G527 methyltransferase, SAM‑dependent; glucose‑inhibited cell‑division protein
RA 3,919,832 T→A 15.0% intergenic (‑17/+73) mioC ← / ← asnC FMN‑binding protein MioC/transcriptional activator of asnA; autorepressor
RA 3,921,982 T→A 5.9% T328S (ACT→TCT)  viaA ← stimulator of RavA ATPase activity; von Willebrand factor domain protein
RA 3,926,774 G→T 11.2% D22Y (GAT→TAT)  rbsD → putative cytoplasmic sugar‑binding protein
RA 3,926,831 G→T 33.8% D41Y (GAT→TAT)  rbsD → putative cytoplasmic sugar‑binding protein
RA 3,927,123 C→A 16.6% T138K (ACG→AAG)  rbsD → putative cytoplasmic sugar‑binding protein
RA 3,927,918 G→T 12.8% D261Y (GAT→TAT)  rbsA → fused D‑ribose transporter subunits of ABC superfamily: ATP‑binding components
RA 3,928,395 G→T 8.7% D420Y (GAT→TAT)  rbsA → fused D‑ribose transporter subunits of ABC superfamily: ATP‑binding components
RA 3,932,409 G→T 12.0% D275Y (GAT→TAT)  rbsR → transcriptional repressor of ribose metabolism
RA 3,952,842 A→C 10.9% intergenic (+37/+50) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
RA 3,960,036 G→A 100% R87H (CGC→CAC)  rho → transcription termination factor
RA 3,966,854 C→A 33.2% R325S (CGT→AGT)  rffG → dTDP‑glucose 4,6‑dehydratase
RA 3,969,710 G→T 20.5% G25C (GGT→TGT)  wzxE → O‑antigen translocase
RA 3,988,075 T→A 30.6% V65E (GTG→GAG)  yifL → putative lipoprotein
RA 3,991,234 T→A 7.5% L231* (TTG→TAG)  yigB → 5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase; pyrimidine phosphatase; riboflavin synthesis
RA 3,994,076 A→T 74.8% L114* (TTA→TAA)  yigE ← DUF2233 family protein
RA 3,994,247 A→T 93.9% V57E (GTG→GAG)  yigE ← DUF2233 family protein
RA 4,009,705 G→A 94.1% intergenic (‑176/‑86) ysgA ← / → udp putative carboxymethylenebutenolidase/uridine phosphorylase
RA 4,037,406 T→A 9.9% intergenic (+2/‑153) dsbA → / → yihF periplasmic protein disulfide isomerase I/DUF945 family protein
RA 4,056,018 T→A 93.4% F138I (TTC→ATC)  yihN → MFS transporter family protein
RA 4,062,424 G→T 91.2% I71I (ATC→ATA yihQ ← alpha‑glucosidase
RA 4,066,133 T→G 93.4% S267R (AGC→CGC)  yihU ← gamma‑hydroxybutyrate dehydrogenase, NADH‑dependent
RA 4,068,208 C→A 100% G60G (GGC→GGA yihW → DeoR family putative transcriptional regulator
RA 4,079,023 A→G 5.1% S54P (TCC→CCC)  fdoG ← formate dehydrogenase‑O, large subunit
RA 4,085,600 G→T 26.9% Q43K (CAA→AAA)  frvB ← putative PTS enzyme, IIB component/IIC component
RA 4,094,362 C→A 92.9% W181L (TGG→TTG)  cpxA ← sensory histidine kinase in two‑component regulatory system with CpxR
RA 4,097,822 G→T 92.8% E115* (GAA→TAA)  pfkA → 6‑phosphofructokinase I
RA 4,118,210 G→T 11.2% T38T (ACC→ACA metJ ← transcriptional repressor, S‑adenosylmethionine‑binding
RA 4,127,022 T→G 92.0% E188D (GAA→GAC yijF ← DUF1287 family protein
RA 4,176,823 G→T 85.7% D516Y (GAC→TAC)  rpoC → RNA polymerase, beta prime subunit
RA 4,177,037 T→A 14.5% L587Q (CTG→CAG)  rpoC → RNA polymerase, beta prime subunit
RA 4,178,401 G→T 12.8% D1042Y (GAC→TAC)  rpoC → RNA polymerase, beta prime subunit
RA 4,187,518 G→T 20.1% D230Y (GAT→TAT)  nudC → NADH pyrophosphatase
RA 4,211,576 G→T 67.9% S280R (AGC→AGA arpA ← ankyrin repeat protein
RA 4,211,803 G→A 89.4% P205S (CCT→TCT)  arpA ← ankyrin repeat protein
RA 4,213,666 C→A 6.4% intergenic (‑110/‑90) iclR ← / → metH transcriptional repressor/homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent
RA 4,230,913 A→T 86.3% W424R (TGG→AGG)  xylE ← D‑xylose transporter
RA 4,242,023 G→T 18.7% pseudogene (1135/1323 nt) yjbI → pseudogene, SopA‑related, pentapeptide repeats‑containing
RA 4,250,324 G→A 22.9% A26T (GCA→ACA)  yjbL → uncharacterized protein
RA 4,265,064 C→A 35.0% intergenic (‑95/‑335) yjcB ← / → yjcC putative inner membrane protein/putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
RA 4,271,025 T→A 13.0% I373N (ATC→AAC)  yjcE → putative cation/proton antiporter
RA 4,274,926 A→C 73.5% I72M (ATT→ATG yjcH ← DUF485 family inner membrane protein
RA 4,279,051 G→T 15.0% E454* (GAG→TAG)  nrfA → nitrite reductase, formate‑dependent, cytochrome
RA 4,290,080 G→T 88.1% I256I (ATC→ATA mdtP ← outer membrane factor of efflux pump
RA 4,297,846 C→A 91.2% M150I (ATG→ATT alsE ← allulose‑6‑phosphate 3‑epimerase
RA 4,303,614 T→A 86.2% *150R (TGA→AGA)  rpiB → ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase
RA 4,306,969 T→A 10.8% N23Y (AAC→TAC)  phnM ← ribophosphonate triphosphate hydrolase
RA 4,312,246 C→A 17.6% intergenic (‑7/+14) phnF ← / ← phnE putative DNA‑binding transcriptional regulator of phosphonate uptake and biodegradation/defective phosphonate ABC transporter permease;transport; Transport of small molecules: Anions; membrane channel protein component of Pn transporter; membrane component of ABC superfamily
RA 4,312,726 G→T 23.4% pseudogene (322/426 nt) phnE ← defective phosphonate ABC transporter permease;transport; Transport of small molecules: Anions; membrane channel protein component of Pn transporter; membrane component of ABC superfamily
RA 4,315,964 T→C 6.3% intergenic (‑406/+252) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,341,023 A→T 32.9% Y153N (TAC→AAC)  dcuS ← sensory histidine kinase in two‑component regulatory system with DcuR, regulator of anaerobic fumarate respiration
RA 4,344,298 G→T 5.8% I79I (ATC→ATA lysU ← lysine tRNA synthetase, inducible
RA 4,353,585 C→T 6.2% L425L (TTG→TTA dsbD ← fused thiol:disulfide interchange protein: activator of DsbC/conserved protein
RA 4,356,174 A→T 27.4% I139I (ATT→ATA dcuA ← C4‑dicarboxylate antiporter
RA 4,365,977 T→A 9.7% A154A (GCT→GCA efp → polyproline‑specific translation elongation factor EF‑P
RA 4,382,806 T→A 12.6% Q360L (CAA→CTA)  queG ← epoxyqueuosine reductase, cobalamine‑stimulated; queosine biosynthesis
RA 4,388,902 G→T 93.8% A558A (GCG→GCT mutL → methyl‑directed mismatch repair protein
RA 4,396,598 A→T 92.8% E43V (GAA→GTA)  rnr → exoribonuclease R, RNase R
RA 4,417,551 A→C 29.9% pseudogene (41/402 nt) ytfA → pseudogene, related to transcriptional regulators
RA 4,432,682 G→T 20.9% E162* (GAA→TAA)  tamA → translocation and assembly module for autotransporter export, outer membrane subunit
RA 4,458,254 T→A 92.8% V271V (GTT→GTA mgtA → magnesium transporter
RA 4,458,609 G→T 93.2% D390Y (GAT→TAT)  mgtA → magnesium transporter
RA 4,460,286 G→T 6.0% intergenic (+148/+58) mgtA → / ← ridA magnesium transporter/enamine/imine deaminase, reaction intermediate detoxification
RA 4,461,726 C→A 6.3% D163Y (GAC→TAC)  pyrB ← aspartate carbamoyltransferase, catalytic subunit
RA 4,464,572 G→T 11.2% intergenic (+38/‑107) bdcR → / → tabA transcriptional repressor for divergent bdcA/biofilm modulator regulated by toxins; DUF386 family protein, cupin superfamily
RA 4,468,165 A→G 57.7% intergenic (‑37/‑125) argI ← / → rraB ornithine carbamoyltransferase 1/protein inhibitor of RNase E
RA 4,469,550 G→A 85.7% A2T (GCT→ACT)  yjgN → DUF898 family inner membrane protein
RA 4,470,017 G→A 93.6% M157I (ATG→ATA yjgN → DUF898 family inner membrane protein
RA 4,471,490 G→T 5.3% P722Q (CCG→CAG)  valS ← valyl‑tRNA synthetase
RA 4,489,542 A→T 13.7% pseudogene (810/942 nt) yjgX ← pseudogene fragment
RA 4,505,438 C→A 6.8% D353Y (GAC→TAC)  fecA ← ferric citrate outer membrane transporter
RA 4,506,776 C→A 10.9% P253P (CCG→CCT fecR ← FecI pro‑sigma factor; transmembrane signal transducer for ferric citrate transport; periplasmic FecA:ferric citrate sensor and cytoplasmic FecI ECF sigma factor activator
RA 4,511,877 G→A 16.8% intergenic (‑40/+67) yjhF ← / ← yjhG putative transporter/putative dehydratase
RA 4,512,693 T→A 100% K407* (AAG→TAG)  yjhG ← putative dehydratase
RA 4,516,734 G→T 26.7% F207L (TTC→TTA sgcE ← putative epimerase
RA 4,525,234 G→A 87.5% pseudogene (415/1029 nt) yjhR → pseudogene, helicase family; putative frameshift suppressor
RA 4,531,143 A→T 100% I124F (ATT→TTT)  fimB → tyrosine recombinase/inversion of on/off regulator of fimA
RA 4,534,644 C→A 6.2% S175Y (TCT→TAT)  fimC → periplasmic chaperone
RA 4,555,156 G→T 33.6% A171E (GCG→GAG)  yjiM ← putative 2‑hydroxyglutaryl‑CoA dehydratase
RA 4,556,296 C→T 14.6% Q256Q (CAG→CAA yjiN ← zinc‑type alcohol dehydrogenase‑like protein
RA 4,558,657 G→T 6.1% intergenic (‑321/‑158) mdtM ← / → yjiP multidrug efflux system protein/pseudogene, transposase_31 family protein
RA 4,562,043 C→A 93.6% intergenic (+311/‑188) yjiS → / → yjiT DUF1127 family protein/pseudogene
RA 4,584,381 G→T 94.0% I53I (ATC→ATA yjjL ← L‑galactonate transporter
RA 4,585,338 A→T 32.3% S111T (TCT→ACT)  yjjM ← yjjMN operon transcriptional activator
RA 4,601,014 T→C 11.7% intergenic (+194/‑199) prfC → / → osmY peptide chain release factor RF‑3/periplasmic protein
RA 4,604,616 C→A 8.2% G249C (GGC→TGC)  yjjW ← putative pyruvate formate lyase activating enzyme
RA 4,605,290 A→T 11.1% L24Q (CTG→CAG)  yjjW ← putative pyruvate formate lyase activating enzyme
JC 4,618,549 Δ116 bp 5.8% intergenic (+185/+8) nadR → / ← yjjK trifunctional protein: nicotinamide mononucleotide adenylyltransferase, ribosylnicotinamide kinase, transcriptional repressor/putative transporter subunit of ABC superfamily: ATP‑binding component
RA 4,622,314 T→A 7.2% W589R (TGG→AGG)  slt → lytic murein transglycosylase, soluble
RA 4,625,288 T→C 27.0% intergenic (‑161/‑50) rob ← / → creA right oriC‑binding transcriptional activator, AraC family/putative periplasmic protein
RA 4,626,235 G→T 10.5% E138* (GAA→TAA)  creB → response regulator in two‑component regulatory system with CreC

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 373834 375142 1309 150 [147] [147] 149 frmA–[frmR] frmA,[frmR]
* * ÷ CP009273 4100613 4101509 897 151 [144] [148] 150 [cdh]–tpiA [cdh],tpiA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 360104 =162 (0.670)178 (0.740) 102/278 NT 52.4% intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 = 360278NA (NA)38325 (168.860)
+TTAATTAA
204/262 NT 99.5% intergenic (‑212/+383) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 361823 218 (0.900)coding (831/834 nt) mhpR mhp operon transcriptional activator
* ? CP009273 360652 =207 (0.860)38174 (237.660)
+46 bp
186/186 NT 99.6% intergenic (‑586/+9) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 1317577 210 (0.870)coding (101/882 nt) yciV PHP domain protein
* ? CP009273 = 361823218 (0.900)22 (0.100)
+TTAATTAA
13/262 NT 10.1% coding (831/834 nt) mhpR mhp operon transcriptional activator
?CP009273 4033991 = 200 (0.830)noncoding (115/120 nt) rrfA 5S ribosomal RNA of rrnA operon
* ? CP009273 376714 =NA (NA)5 (0.020) 5/276 NT NA pseudogene (2/416 nt) yaiX pseudogene, interrupted by IS2A, acetyltransferase homolog; nonfunctional; interruped by IS2; putative transferase
?CP009273 = 378045 NA (NA)intergenic (+10/+2) insD1/yaiX IS2 transposase TnpB/pseudogene, interrupted by IS2A, acetyltransferase homolog; nonfunctional; interruped by IS2; putative transferase
* ? CP009273 376716 =NA (NA)31 (0.130) 22/278 NT 15.7% intergenic (‑1/‑91) yaiX/insC1 pseudogene, interrupted by IS2A, acetyltransferase homolog; nonfunctional; interruped by IS2; putative transferase/IS2 repressor TnpA
?CP009273 = 386403 167 (0.690)pseudogene (697/1458 nt) yaiT pseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein
* ? CP009273 376716 =NA (NA)225 (0.930) 125/278 NT 96.6% intergenic (‑1/‑91) yaiX/insC1 pseudogene, interrupted by IS2A, acetyltransferase homolog; nonfunctional; interruped by IS2; putative transferase/IS2 repressor TnpA
?CP009273 566411 = 8 (0.030)coding (63/552 nt) ybcL inactive polymorphonuclear leukocyte migration suppressor; DLP12 prophage; secreted protein, UPF0098 family
* ? CP009273 = 378046NA (NA)41 (0.170) 31/278 NT 19.4% intergenic (+11/+1) insD1/yaiX IS2 transposase TnpB/pseudogene, interrupted by IS2A, acetyltransferase homolog; nonfunctional; interruped by IS2; putative transferase
?CP009273 386399 = 170 (0.710)pseudogene (693/1458 nt) yaiT pseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein
* ? CP009273 = 378046NA (NA)184 (0.760) 111/278 NT 95.8% intergenic (+11/+1) insD1/yaiX IS2 transposase TnpB/pseudogene, interrupted by IS2A, acetyltransferase homolog; nonfunctional; interruped by IS2; putative transferase
?CP009273 = 566415 8 (0.030)coding (67/552 nt) ybcL inactive polymorphonuclear leukocyte migration suppressor; DLP12 prophage; secreted protein, UPF0098 family
* ? CP009273 776825 =137 (0.570)22 (0.090)
+G
6/276 NT 12.2% noncoding (1/76 nt) lysZ tRNA‑Lys
?CP009273 = 2514488 181 (0.750)noncoding (216/1345 nt) IS186 repeat region
* ? CP009273 1203246 =16 (0.070)190 (0.890) 114/246 NT 92.7% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 1205075 = 16 (0.080)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 120326117 (0.070)196 (0.920) 110/246 NT 92.7% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 16 (0.080)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 1462167 =NA (NA)11 (0.050) 10/278 NT NA noncoding (1331/1331 nt) IS2 repeat region
?CP009273 = 1463498 NA (NA)pseudogene (1/53 nt) ydbA pseudogene, autotransporter homolog; interrupted by IS2 and IS30
* ? CP009273 1462167 =NA (NA)10 (0.040) 9/278 NT NA noncoding (1331/1331 nt) IS2 repeat region
?CP009273 = 1463499 NA (NA)pseudogene (2/53 nt) ydbA pseudogene, autotransporter homolog; interrupted by IS2 and IS30
* ? CP009273 1635219 =7 (0.030)158 (0.670) 95/274 NT 95.8% intergenic (‑377/+377) essQ/cspB Qin prophage; putative S lysis protein/Qin prophage; cold shock protein
?CP009273 = 2063752 NA (NA)noncoding (1/1331 nt) IS2 repeat region
* ? CP009273 = 16352236 (0.020)200 (0.830) 113/278 NT 97.1% intergenic (‑381/+373) essQ/cspB Qin prophage; putative S lysis protein/Qin prophage; cold shock protein
?CP009273 = 1645805 NA (NA)intergenic (+11/‑3) insD1/intQ IS2 transposase TnpB;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related/pseudogene, Qin prophage; phage integrase family;Phage or Prophage Related
* ? CP009273 = 1645804NA (NA)21 (0.090) 17/276 NT 9.8% intergenic (+10/‑4) insD1/intQ IS2 transposase TnpB;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related/pseudogene, Qin prophage; phage integrase family;Phage or Prophage Related
?CP009273 = 2309096 194 (0.810)coding (2130/2673 nt) rcsD phosphotransfer intermediate protein in two‑component regulatory system with RcsBC
* ? CP009273 2309091 =189 (0.780)45 (0.190) 31/278 NT 19.2% coding (2125/2673 nt) rcsD phosphotransfer intermediate protein in two‑component regulatory system with RcsBC
?CP009273 = 2991050 NA (NA)noncoding (1/1331 nt) IS2 repeat region
* ? CP009273 2989719 =NA (NA)14 (0.060)
+C
6/276 NT NA pseudogene (1/399 nt) ygeO pseudogene, orgA homolog, part of T3SS PAI ETT2 remnant
?CP009273 = 2991050 NA (NA)noncoding (1/1331 nt) IS2 repeat region
* ? CP009273 3179453 =NA (NA)4 (0.020) 3/278 NT NA intergenic (+5/‑93) yqiG/insC1 pseudogene; fimbrial export usher family;putative membrane; Not classified; putative membrane protein/IS2 repressor TnpA
?CP009273 = 3180785 NA (NA)pseudogene (6/2445 nt) yqiG pseudogene; fimbrial export usher family;putative membrane; Not classified; putative membrane protein