Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F181 I0 R1 1262 323.1 10297870 97.5% 10040423 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,814,302 T→A 29.1% S77C (AGC→TGC)  ligB ← DNA ligase, NAD(+)‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,814,3020TA29.1% 508.5 / 278.4 295S77C (AGC→TGC) ligBDNA ligase, NAD(+)‑dependent
Reads supporting (aligned to +/- strand):  ref base T (105/104);  new base A (46/40);  total (151/144)
Fisher's exact test for biased strand distribution p-value = 7.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

Only 100 of 320 total aligned reads displayed.

ATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGAAATTTCTTCCTGTGCTCTGGCTG  >  CP009273/3814153‑3814451
                                                                                                                                                     |                                                                                                                                                     
  cAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCAcc                                                                                                                                                     >  2:679426/1‑150 (MQ=255)
     cTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCaa                                                                                                                                                  >  2:2467941/1‑150 (MQ=255)
      ttttGGCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCaaa                                                                                                                                                 <  1:3178930/150‑1 (MQ=255)
         tGGCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCACCCAAagca                                                                                                                                              >  1:5055119/1‑150 (MQ=255)
                   cAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCAc                                                                                                                                    <  1:2931336/150‑1 (MQ=255)
                    aaaGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACt                                                                                                                                   <  2:166108/150‑1 (MQ=255)
                      aGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGc                                                                                                                                 <  1:4528849/150‑1 (MQ=255)
                      aGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGc                                                                                                                                 <  1:1005287/150‑1 (MQ=255)
                       gATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCg                                                                                                                                <  1:4901190/150‑1 (MQ=255)
                        aTCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGt                                                                                                                               >  2:1322180/1‑150 (MQ=255)
                            cTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGa                                                                                                                           >  2:4792120/1‑150 (MQ=255)
                            cTACGTTCTCGCATCCACAGACTTAATGCGTTTTAATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGAGGCATCATCACGTCTCGGGGCTCGCTCACAAAGCAGCGTTGACACTGCGTAAgt                                                                                                                           >  2:4444053/1‑149 (MQ=255)
                               cGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACgg                                                                                                                        <  1:3001062/150‑1 (MQ=255)
                               cGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCACCCAAAGCAGCGTTGCCACTGCGTAAGACgg                                                                                                                        <  1:4160265/150‑1 (MQ=255)
                                  tctcGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGgcg                                                                                                                     <  1:3356570/150‑1 (MQ=255)
                                      gCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTa                                                                                                                 >  1:1486276/1‑150 (MQ=255)
                                           cacaGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGa                                                                                                            >  2:53177/1‑150 (MQ=255)
                                           cacaGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGa                                                                                                            >  1:619121/1‑150 (MQ=255)
                                               gACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCACCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGt                                                                                                        >  2:1186634/1‑150 (MQ=255)
                                                  ttAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTaaa                                                                                                     <  1:2319299/150‑1 (MQ=255)
                                                   tAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAac                                                                                                    <  2:103230/150‑1 (MQ=255)
                                                    aaTGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAaca                                                                                                   <  1:910104/150‑1 (MQ=255)
                                                    aaTGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAaca                                                                                                   >  1:1978492/1‑150 (MQ=255)
                                                    aaTGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAaca                                                                                                   >  2:5013630/1‑150 (MQ=255)
                                                     aTGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCACCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAacac                                                                                                  >  1:2403266/1‑150 (MQ=255)
                                                      tGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACAcc                                                                                                 <  2:1892889/150‑1 (MQ=255)
                                                      tGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCACCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACAcc                                                                                                 <  2:1546684/150‑1 (MQ=255)
                                                        cGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCAGCACGTCGCGGGGCTCGCACCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGt                                                                                               >  2:3568308/1‑150 (MQ=255)
                                                              tATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCa                                                                                         >  2:2053258/1‑150 (MQ=255)
                                                                tCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCAcc                                                                                       >  1:694313/1‑150 (MQ=255)
                                                                tCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCACCCAAAGCAGCGTTGCCACTGCGTAAGGCGGGCGCTTAACTGAGCGTAAACACCGTCTTCCAcc                                                                                       >  2:3648794/1‑150 (MQ=255)
                                                                  caccaTTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTc                                                                                     >  1:1389841/1‑150 (MQ=255)
                                                                     caTTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCACCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACt                                                                                  <  2:234439/150‑1 (MQ=255)
                                                                       ttttGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACttt                                                                                <  1:364054/150‑1 (MQ=255)
                                                                       ttttGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACttt                                                                                >  1:4844078/1‑150 (MQ=255)
                                                                        tttGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACtttt                                                                               <  1:1498328/150‑1 (MQ=255)
                                                                        tttGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCACCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACtttt                                                                               >  1:1053710/1‑150 (MQ=255)
                                                                         ttGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACttttt                                                                              >  2:739159/1‑150 (MQ=255)
                                                                         ttGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACttttt                                                                              <  2:1376692/150‑1 (MQ=255)
                                                                          tGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTttc                                                                             <  1:4200293/150‑1 (MQ=255)
                                                                          tGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCACCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTttc                                                                             >  1:3887106/1‑150 (MQ=255)
                                                                           gCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTttcc                                                                            <  1:3585115/150‑1 (MQ=255)
                                                                              tACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTttccttc                                                                         <  1:3086527/150‑1 (MQ=255)
                                                                               acacCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTttccttcc                                                                        <  2:148280/150‑1 (MQ=255)
                                                                                  cccGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTttccttccttc                                                                     >  1:2592907/1‑150 (MQ=255)
                                                                                   ccGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCACCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTttccttccttcc                                                                    <  2:4415830/150‑1 (MQ=255)
                                                                                     gTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCag                                                                  >  2:4529212/1‑150 (MQ=255)
                                                                                      tATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCagt                                                                 >  1:1699985/1‑150 (MQ=255)
                                                                                      tATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCACCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCagt                                                                 <  2:2645467/150‑1 (MQ=255)
                                                                                         gggCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCagtagt                                                              >  1:2813166/1‑150 (MQ=255)
                                                                                          ggCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTc                                                             <  2:405993/150‑1 (MQ=255)
                                                                                          ggCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTc                                                             <  2:2144634/150‑1 (MQ=255)
                                                                                          ggCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTc                                                             <  1:1917460/150‑1 (MQ=255)
                                                                                            cAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCAt                                                           <  2:334330/150‑1 (MQ=255)
                                                                                             aaCCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATc                                                          >  2:4490575/1‑150 (MQ=255)
                                                                                             aaCCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATc                                                          <  1:4630516/150‑1 (MQ=255)
                                                                                             aaCCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATc                                                          >  1:115686/1‑150 (MQ=255)
                                                                                             aaCCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATc                                                          >  2:1936618/1‑150 (MQ=255)
                                                                                             aaCCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATc                                                          >  2:5000172/1‑150 (MQ=255)
                                                                                              aCCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCACCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCg                                                         >  1:1135927/1‑150 (MQ=255)
                                                                                                cGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTc                                                       >  1:1120568/1‑150 (MQ=255)
                                                                                                cGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTc                                                       >  2:4659740/1‑150 (MQ=255)
                                                                                                cGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTc                                                       >  1:1255195/1‑150 (MQ=255)
                                                                                                cGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCACCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGGCTTCCACCTCACTGTTTCCTTCCTTCCCGTAGTCATCGTc                                                       >  1:270252/1‑150 (MQ=255)
                                                                                                 ggATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCACCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTcc                                                      <  2:1829206/150‑1 (MQ=255)
                                                                                                   aTGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCa                                                    <  1:1705952/150‑1 (MQ=255)
                                                                                                    tGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCAc                                                   >  2:4951553/1‑150 (MQ=255)
                                                                                                      cATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCACCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTg                                                 >  1:1292656/1‑150 (MQ=255)
                                                                                                        tAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGtt                                               >  2:3080686/1‑150 (MQ=255)
                                                                                                            cGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCACCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTAt                                           <  2:4581284/150‑1 (MQ=255)
                                                                                                             gCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCACCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTAtt                                          <  1:2433133/150‑1 (MQ=255)
                                                                                                                ccATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCACCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGc                                       >  1:527878/1‑150 (MQ=255)
                                                                                                                   tttAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGt                                    <  2:1534046/150‑1 (MQ=255)
                                                                                                                    ttAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACTGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGtt                                   <  2:3906670/150‑1 (MQ=255)
                                                                                                                    ttAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGtt                                   <  1:2150942/150‑1 (MQ=255)
                                                                                                                      aaaGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGc                                 >  2:855635/1‑150 (MQ=255)
                                                                                                                      aaaGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGc                                 <  2:3196562/150‑1 (MQ=255)
                                                                                                                       aaGGCGGCATCATCACGTCGCGGGGCTCGCACCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCa                                >  2:1688235/1‑150 (MQ=255)
                                                                                                                         ggcggcATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAgg                              >  2:5115791/1‑150 (MQ=255)
                                                                                                                           cggcATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCg                            >  1:888171/1‑150 (MQ=255)
                                                                                                                           cggcATCATCACGTCGCGGGGCTCGCACCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCg                            <  2:3449146/150‑1 (MQ=255)
                                                                                                                                tcatcaCGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGaaa                       >  2:2538137/1‑150 (MQ=255)
                                                                                                                                  atcaCGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACTGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTAGCAGGCGGGAAAtt                     <  2:1982818/150‑1 (MQ=255)
                                                                                                                                        tCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGAAATTTCTTcc               <  1:2280788/150‑1 (MQ=255)
                                                                                                                                          gcgGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGAAATTTCTTCCtg             <  2:4276172/150‑1 (MQ=255)
                                                                                                                                          gcgGGGCTCGCACCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGAAATTTCTTCCtg             <  1:4833107/150‑1 (MQ=255)
                                                                                                                                           cgGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGAAATTTCTTCCtgt            >  2:275756/1‑150 (MQ=255)
                                                                                                                                            ggggCTCGCACCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGAAATTTCTTCCtgtg           >  2:4107029/1‑150 (MQ=255)
                                                                                                                                             gggCTCGCTCCCAACGCAGCGGTGCCACTGCGTAAGACGGGCGCTTCACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGAAATTTCTTCCTGTGc          <  2:358321/150‑1 (MQ=255)
                                                                                                                                             gggCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGAAATTTCTTCCTGTGc          >  1:4499438/1‑150 (MQ=255)
                                                                                                                                              ggCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGAAATTTCTTCCTGTGct         <  1:4982395/150‑1 (MQ=255)
                                                                                                                                              ggCTCGCACCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGAAATTTCTTCCTGTGct         >  1:3225223/1‑150 (MQ=255)
                                                                                                                                                ctcgctcCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGAAATTTCTTCCTGTGctct       >  1:2303893/1‑150 (MQ=255)
                                                                                                                                                 tcgctcCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGAAATTTCTTCCTGTGCTctg      >  1:4041241/1‑150 (MQ=255)
                                                                                                                                                  cgctcCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGAAATTTCTTCCTGTGCTctgg     <  1:592324/150‑1 (MQ=255)
                                                                                                                                                  cgctcCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGAAATTTCTTCCTGTGCTctgg     <  1:1613429/150‑1 (MQ=255)
                                                                                                                                                  cgcacCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGAAATTTCTTCCTGTGCTctgg     <  2:812424/150‑1 (MQ=255)
                                                                                                                                                     tcCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGAAATTTCTTCCTGTGCTctggctg  >  1:3680153/1‑150 (MQ=255)
                                                                                                                                                     tcCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGAAATTTCTTCCTGTGCTctggctg  >  2:2232540/1‑150 (MQ=255)
                                                                                                                                                     tcCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGAAATTTCTTCCTGTGCTctggctg  <  1:2053258/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATCAACTTTTGGCTGCACCCAAAGATCGCTACGTTCTCGCATCCACAGACTTAATGCGTTTTTATCCACCATTTTGCGTACACCCGTATGGGCAACCGGATGCATAACCGCACCATTTAAAGGCGGCATCATCACGTCGCGGGGCTCGCTCCCAAAGCAGCGTTGCCACTGCGTAAGACGGGCGCTTAACTGATCGTAAACACCGTCTTCCACCTCACTTTTTCCTTCCTTCCAGTAGTCATCGTCCCACTGTTTTATTTGCTGTTGCAGGCGGGAAATTTCTTCCTGTGCTCTGGCTG  >  CP009273/3814153‑3814451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A