Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 215,833 G→T 12.5% N83K (AAC→AAA)  tsaA ← tRNA‑Thr(GGU) m(6)t(6)A37 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273215,8330GT12.5% 1042.5 / 78.5 312N83K (AAC→AAA) tsaAtRNA‑Thr(GGU) m(6)t(6)A37 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base G (125/147);  new base T (19/20);  total (144/168)
Fisher's exact test for biased strand distribution p-value = 8.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 318 total aligned reads displayed.

ACTACCGGCGTACCATCGACCAGATCCAGACTGCCGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCC  >  CP009273/215684‑215982
                                                                                                                                                     |                                                                                                                                                     
aCTACCGGCGTACCATCGACCAGATCCAGACTGCCGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCg                                                                                                                                                       >  2:3223850/1‑150 (MQ=255)
aCTACCGGCGTACCATCGACCAGATCCAGACTGCCGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCg                                                                                                                                                       >  2:4220256/1‑150 (MQ=255)
 cTACCGGCGTACCATCGACCAGATCCAGACTGCCGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGt                                                                                                                                                      <  1:3983801/150‑1 (MQ=255)
   aCCGGCGTACCATCGACCAGATCCAGACTGCCGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTa                                                                                                                                                    <  2:3483745/150‑1 (MQ=255)
   aCCGGCGTACCATCGACCAGATCCAGACTGCCGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTa                                                                                                                                                    <  2:433821/150‑1 (MQ=255)
    ccGGCGTACCATCGACCAGATCCAGACTGCCGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTAc                                                                                                                                                   <  1:343676/150‑1 (MQ=255)
         gTACCATCGACCAGATCCAGACTGCCGAGCTTCATAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTAccgccg                                                                                                                                              <  2:534078/150‑1 (MQ=255)
         gTACCATCGACCAGATCCAGACTGCCGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTAccgccg                                                                                                                                              >  2:1782432/1‑150 (MQ=255)
         gTACCATCGACCAGATCCAGACTGCCGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTAccgccg                                                                                                                                              <  2:2819225/150‑1 (MQ=255)
         gTACCATCGACCAGATCCAGACTGCCGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTAccgccg                                                                                                                                              >  1:35980/1‑150 (MQ=255)
          tACCATCGACCAGATCCAGACTGCCGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGa                                                                                                                                             <  2:1604244/150‑1 (MQ=255)
            ccATCGACCAGATCCAGACTGCCGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAgg                                                                                                                                           >  1:3832486/1‑150 (MQ=255)
            ccATCGACCAGATCCAGACTGCCGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAgg                                                                                                                                           <  2:4239710/150‑1 (MQ=255)
              aTCGACCAGATCCAGACTGCCGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGgcg                                                                                                                                         >  2:4251579/1‑150 (MQ=255)
                  aCCAGATCCAGACTGCCGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGt                                                                                                                                     >  2:659074/1‑150 (MQ=255)
                   ccAGATCCAGACTGCCGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTg                                                                                                                                    <  1:3164937/150‑1 (MQ=255)
                     aGATCCAGACTGCCGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGa                                                                                                                                  >  1:3033814/1‑150 (MQ=255)
                      gATCCAGACTGCCGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGAc                                                                                                                                 <  1:790856/150‑1 (MQ=255)
                       aTCCAGACTGCCGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACg                                                                                                                                <  1:3523691/150‑1 (MQ=255)
                          cAGACTGCCGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCTTTACCGCCGAGGCGCGGTGGACGcac                                                                                                                             <  2:2934340/150‑1 (MQ=255)
                          cAGACTGCCGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGcac                                                                                                                             <  1:2922650/150‑1 (MQ=255)
                             aCTGCCGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGt                                                                                                                          >  1:320544/1‑150 (MQ=255)
                               tGCCGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCg                                                                                                                        >  1:2148403/1‑150 (MQ=255)
                                gCCGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCgg                                                                                                                       <  2:2524001/150‑1 (MQ=255)
                                 ccGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGATCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCTCAGTCGGa                                                                                                                      <  2:2180011/150‑1 (MQ=255)
                                  cGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCTTTACCGCCGAGGCGCGGTGGACGCACAGTCGGAc                                                                                                                     <  1:1358413/150‑1 (MQ=255)
                                    aGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGc                                                                                                                   >  2:1968226/1‑150 (MQ=255)
                                      cTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCa                                                                                                                 <  1:4467697/150‑1 (MQ=255)
                                      cTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCa                                                                                                                 >  2:2598275/1‑150 (MQ=255)
                                         cAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAgcc                                                                                                              <  2:2069151/150‑1 (MQ=255)
                                              tCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCTTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCtt                                                                                                         >  1:1830803/1‑150 (MQ=255)
                                               cACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTc                                                                                                        >  1:2699119/1‑150 (MQ=255)
                                                aCGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTcc                                                                                                       >  2:4372383/1‑150 (MQ=255)
                                                aCGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTcc                                                                                                       <  1:2541189/150‑1 (MQ=255)
                                                aCGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTcc                                                                                                       >  1:4049357/1‑150 (MQ=255)
                                                aCGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTcc                                                                                                       >  2:4370775/1‑150 (MQ=255)
                                                 cGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCa                                                                                                      >  1:3641643/1‑150 (MQ=255)
                                                   cTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATc                                                                                                    <  1:4381635/150‑1 (MQ=255)
                                                    tGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCg                                                                                                   >  2:1037839/1‑150 (MQ=255)
                                                     gTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCCTCCATCGt                                                                                                  >  1:2202198/1‑150 (MQ=255)
                                                       cTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGttt                                                                                                <  2:1683987/150‑1 (MQ=255)
                                                       cTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGttt                                                                                                >  2:1463098/1‑150 (MQ=255)
                                                           aTGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTtgatg                                                                                            <  2:3809945/150‑1 (MQ=255)
                                                           aTGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTtgatg                                                                                            <  2:3812151/150‑1 (MQ=255)
                                                            tGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTtgatga                                                                                           <  2:3417044/150‑1 (MQ=255)
                                                            tGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTtgatga                                                                                           <  2:1026658/150‑1 (MQ=255)
                                                             ggCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGaa                                                                                          >  1:3957232/1‑150 (MQ=255)
                                                               cAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAg                                                                                        <  1:3439512/150‑1 (MQ=255)
                                                                aaaCAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCTTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGa                                                                                       <  2:37162/150‑1 (MQ=255)
                                                                aaaCAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGa                                                                                       >  1:1908048/1‑150 (MQ=255)
                                                                 aacaacCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGAc                                                                                      <  1:1670508/150‑1 (MQ=255)
                                                                  acaacCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACg                                                                                     >  1:2078292/1‑150 (MQ=255)
                                                                  acaacCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACg                                                                                     <  2:3973143/150‑1 (MQ=255)
                                                                      ccTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGaaaa                                                                                 <  2:837692/150‑1 (MQ=255)
                                                                      ccTCTTTCAGCTCGACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGaaaa                                                                                 <  2:837650/150‑1 (MQ=255)
                                                                            tCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCTTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATcc                                                                           <  2:3828419/150‑1 (MQ=255)
                                                                             cAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCa                                                                          >  1:2256371/1‑150 (MQ=255)
                                                                               gCTCTTCCAGCGACATGCCATTTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATa                                                                        <  2:2880908/150‑1 (MQ=255)
                                                                               gCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATa                                                                        <  2:1797006/150‑1 (MQ=255)
                                                                                ctctACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCTTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATaa                                                                       >  2:3341295/1‑150 (MQ=255)
                                                                                  ctACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAAt                                                                     <  1:1497868/150‑1 (MQ=255)
                                                                                   tACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATg                                                                    <  1:3462764/150‑1 (MQ=255)
                                                                                   tACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATg                                                                    <  1:3496479/150‑1 (MQ=255)
                                                                                          gACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAAc                                                             >  2:2477222/1‑150 (MQ=255)
                                                                                          gACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAAc                                                             >  2:4398294/1‑150 (MQ=255)
                                                                                              tGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCtt                                                         <  2:2125022/150‑1 (MQ=255)
                                                                                                 cAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCa                                                      >  1:3325465/1‑150 (MQ=255)
                                                                                                   aTTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCTTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAgg                                                    <  1:654954/150‑1 (MQ=255)
                                                                                                   aTTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAgg                                                    >  1:2581359/1‑150 (MQ=255)
                                                                                                   aTTGGGTTAGGGCGGAAAGTAGAGCGTGCTGCGAAAACCCCCATTCTGGCTTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAgg                                                    <  1:463474/150‑1 (MQ=255)
                                                                                                       ggTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAATAGGATCCATAAATGGCTGAACGCTTCCAGGCcgc                                                >  2:3174625/1‑150 (MQ=255)
                                                                                                       ggTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCcgc                                                >  2:3102574/1‑150 (MQ=255)
                                                                                                           aGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAAc                                            <  1:3074596/150‑1 (MQ=255)
                                                                                                            gggCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATTAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACg                                           <  1:3718528/150‑1 (MQ=255)
                                                                                                               cGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGTCTGAACGCTTCCAGGCCGCGAACGGCg                                        >  1:780231/1‑150 (MQ=255)
                                                                                                               cGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCg                                        >  1:3063589/1‑150 (MQ=255)
                                                                                                                 gAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTc                                      >  1:2948392/1‑150 (MQ=255)
                                                                                                                 gAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTc                                      >  1:2948895/1‑150 (MQ=255)
                                                                                                                  aaaGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCg                                     >  2:3045392/1‑150 (MQ=255)
                                                                                                                      tAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCt                                 <  2:679820/150‑1 (MQ=255)
                                                                                                                         agCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGt                              >  2:4095044/1‑150 (MQ=255)
                                                                                                                          gCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGtt                             <  1:451913/150‑1 (MQ=255)
                                                                                                                           cGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTg                            <  1:4293022/150‑1 (MQ=255)
                                                                                                                              gtTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAg                         <  1:5986/150‑1 (MQ=255)
                                                                                                                              gtTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAg                         <  1:6816/150‑1 (MQ=255)
                                                                                                                                  cGAAAACCCCCATTCTGGCGGTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGGTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTGGTAGGGAg                     >  2:3636735/1‑150 (MQ=255)
                                                                                                                                   gAAAACCCCCATTCTGGCTTTACCGCCGAGGCGCGGTGGACGCACAGTCCGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGc                    >  1:1028457/1‑150 (MQ=255)
                                                                                                                                    aaaaCCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCa                   >  2:3203636/1‑150 (MQ=255)
                                                                                                                                     aaaCCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCaa                  <  2:1731728/150‑1 (MQ=255)
                                                                                                                                      aaCCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAAt                 <  1:4280179/150‑1 (MQ=255)
                                                                                                                                       aCCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATg                >  2:2786670/1‑150 (MQ=255)
                                                                                                                                       aCCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCACCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATg                <  1:2317091/150‑1 (MQ=255)
                                                                                                                                         ccccATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATgag              >  1:3365/1‑150 (MQ=255)
                                                                                                                                         ccccATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATgag              >  2:2884820/1‑150 (MQ=255)
                                                                                                                                              ttCTGGCTTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCa         >  1:1693861/1‑150 (MQ=255)
                                                                                                                                              ttCTGGCTTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCGTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCa         >  2:3749848/1‑150 (MQ=255)
                                                                                                                                                cTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGt       >  1:2793604/1‑150 (MQ=255)
                                                                                                                                                 tGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGtt      >  1:1235927/1‑150 (MQ=255)
                                                                                                                                                  ggCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTc     >  2:801993/1‑150 (MQ=255)
                                                                                                                                                  ggCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTc     <  2:1908048/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACTACCGGCGTACCATCGACCAGATCCAGACTGCCGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCC  >  CP009273/215684‑215982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A