Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 216,136 G→T 25.8% P116Q (CCA→CAA)  rcsF ← putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273216,1360GT25.8% 649.9 / 172.0 288P116Q (CCA→CAA) rcsFputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base G (109/104);  new base T (54/21);  total (163/125)
Fisher's exact test for biased strand distribution p-value = 1.82e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 290 total aligned reads displayed.

GCGCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAGTCGCCACTGACTTCACCG  >  CP009273/215987‑216285
                                                                                                                                                     |                                                                                                                                                     
  gcTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGc                                                                                                                                                     >  2:852551/1‑150 (MQ=255)
      tttACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTAc                                                                                                                                                 <  2:2705845/150‑1 (MQ=255)
           cAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTg                                                                                                                                            >  2:631108/1‑150 (MQ=255)
                  ggctggcGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACtt                                                                                                                                     <  2:2174545/150‑1 (MQ=255)
                  ggctggcGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACtt                                                                                                                                     <  1:4969918/150‑1 (MQ=255)
                    ctggcGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCg                                                                                                                                   <  1:4188490/150‑1 (MQ=255)
                    ctggcGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCg                                                                                                                                   <  1:1893562/150‑1 (MQ=255)
                     tggcGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCATATTTGCTCGAACTGGAAACAGCTCAATTCGCCGTAATGTAAAGCGCAGAACCGATACATACAGCCTGACGAAAGCAGCCTTGCGAACCGCTGGTGACGTCGc                                                                                                                                  >  2:5187962/1‑150 (MQ=255)
                          cgGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTa                                                                                                                             >  2:3476978/1‑150 (MQ=255)
                           ggAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTAt                                                                                                                            <  1:2815510/150‑1 (MQ=255)
                           ggAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTAt                                                                                                                            <  1:1683663/150‑1 (MQ=255)
                            gAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATg                                                                                                                           <  2:206851/150‑1 (MQ=255)
                            gAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATg                                                                                                                           <  1:4698767/150‑1 (MQ=255)
                              aCGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCa                                                                                                                         >  1:5064808/1‑150 (MQ=255)
                              aCGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCa                                                                                                                         <  2:3674814/150‑1 (MQ=255)
                              aCGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCa                                                                                                                         <  1:3944337/150‑1 (MQ=255)
                                  cGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTaa                                                                                                                     >  1:723625/1‑150 (MQ=255)
                                   gAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAAt                                                                                                                    <  2:1318199/150‑1 (MQ=255)
                                    aaCTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATa                                                                                                                   >  1:3230388/1‑150 (MQ=255)
                                    aaCTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATa                                                                                                                   <  2:4446274/150‑1 (MQ=255)
                                          tCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCAt                                                                                                             <  2:5226836/150‑1 (MQ=255)
                                           cTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCAtt                                                                                                            >  2:892379/1‑150 (MQ=255)
                                                taGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCtt                                                                                                       >  1:824738/1‑150 (MQ=255)
                                                    gcgagcgaATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCAt                                                                                                   <  2:3593796/150‑1 (MQ=255)
                                                     cgagcgaATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTGATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCAtt                                                                                                  <  1:633287/150‑1 (MQ=255)
                                                     cgagcgaATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCAtt                                                                                                  >  2:3935401/1‑150 (MQ=255)
                                                     cgagcgaATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCAtt                                                                                                  >  1:1312282/1‑150 (MQ=255)
                                                      gagcgaATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCAttt                                                                                                 >  2:4003394/1‑150 (MQ=255)
                                                       agcgaATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCAtttt                                                                                                <  2:5084836/150‑1 (MQ=255)
                                                       agcgaATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCAtttt                                                                                                <  2:4214683/150‑1 (MQ=255)
                                                        gcgaATTACGCATATTTGCTCGAACTGGAAACTGCTCATTTCGCCTTAATGTTTAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCTGTAATTCAGCATTGGCTTTCATTTTa                                                                                               <  2:1536277/150‑1 (MQ=255)
                                                           aaTAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTagag                                                                                            <  1:2547845/150‑1 (MQ=255)
                                                            aTAAAGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAgg                                                                                           >  2:3734366/1‑150 (MQ=255)
                                                              aaCGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCg                                                                                         >  2:2800929/1‑150 (MQ=255)
                                                                ctccTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGtt                                                                                       <  2:80886/148‑1 (MQ=255)
                                                                cGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGtt                                                                                       >  1:610852/1‑150 (MQ=255)
                                                                cGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGtt                                                                                       >  1:928648/1‑150 (MQ=255)
                                                                    tATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGAtt                                                                                   <  1:484193/150‑1 (MQ=255)
                                                                      tttGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTg                                                                                 >  2:665285/1‑150 (MQ=255)
                                                                        tGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCa                                                                               >  2:1005857/1‑150 (MQ=255)
                                                                         gCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCAt                                                                              >  1:1099234/1‑150 (MQ=255)
                                                                          cTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATTCAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATc                                                                             >  2:2629141/1‑150 (MQ=255)
                                                                           tCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATcc                                                                            <  2:3177015/150‑1 (MQ=255)
                                                                            cGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCg                                                                           >  1:3437273/1‑150 (MQ=255)
                                                                            cGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCg                                                                           <  1:4834920/150‑1 (MQ=255)
                                                                             gAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGc                                                                          <  2:3309098/150‑1 (MQ=255)
                                                                               aCTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCtt                                                                        >  2:4140668/1‑150 (MQ=255)
                                                                                 tGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTAc                                                                      >  1:5144963/1‑150 (MQ=255)
                                                                                 tGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTAc                                                                      <  2:1926356/150‑1 (MQ=255)
                                                                                  ggAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACg                                                                     >  2:3443678/1‑150 (MQ=255)
                                                                                    aaaCTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTg                                                                   <  1:2744555/150‑1 (MQ=255)
                                                                                      aCTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCg                                                                 <  2:4959894/150‑1 (MQ=255)
                                                                                        tGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGt                                                               >  2:2245415/1‑150 (MQ=255)
                                                                                        tGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGt                                                               <  2:3402033/150‑1 (MQ=255)
                                                                                         gCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGtt                                                              >  2:1247636/1‑150 (MQ=255)
                                                                                         gCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGtt                                                              <  2:3860972/150‑1 (MQ=255)
                                                                                          cTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTg                                                             >  1:2614647/1‑150 (MQ=255)
                                                                                           tCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTACGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTgg                                                            >  1:2234330/1‑150 (MQ=255)
                                                                                             aTTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGaa                                                          <  1:3125312/150‑1 (MQ=255)
                                                                                               ttCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATg                                                        >  1:1479295/1‑150 (MQ=255)
                                                                                                  gCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTc                                                     <  2:1606818/150‑1 (MQ=255)
                                                                                                   ccGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTcg                                                    <  1:5046516/150‑1 (MQ=255)
                                                                                                    cGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTcgg                                                   >  1:3012204/1‑150 (MQ=255)
                                                                                                    cGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTcgg                                                   >  2:1094539/1‑150 (MQ=255)
                                                                                                     gTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTcggc                                                  >  1:5132866/1‑150 (MQ=255)
                                                                                                     gTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTcggc                                                  <  1:5196224/150‑1 (MQ=255)
                                                                                                      tAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTcggcg                                                 >  1:3180275/1‑150 (MQ=255)
                                                                                                        aTGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGga                                               <  2:528639/150‑1 (MQ=255)
                                                                                                        aTGTTAAGCGCAGAACCGATAAATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGAGTTGATTTGAATCCGCTTACTTGCGGTTGGAATTCTCGGCGga                                               >  1:325710/1‑150 (MQ=255)
                                                                                                         tGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGgag                                              >  2:2514424/1‑150 (MQ=255)
                                                                                                          gTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGgaga                                             >  2:4511015/1‑150 (MQ=255)
                                                                                                            tAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGt                                           >  2:4022784/1‑150 (MQ=255)
                                                                                                            tAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGt                                           <  2:3243033/150‑1 (MQ=255)
                                                                                                            tAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGt                                           <  1:4583693/150‑1 (MQ=255)
                                                                                                                cgcAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTg                                       <  2:4842692/150‑1 (MQ=255)
                                                                                                                   aGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCGGAtt                                    >  1:2342018/1‑150 (MQ=255)
                                                                                                                     aaCCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCAGTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTag                                  >  2:141865/1‑150 (MQ=255)
                                                                                                                     aaCCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTag                                  <  2:2934444/150‑1 (MQ=255)
                                                                                                                     aaCCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTag                                  <  1:1205575/150‑1 (MQ=255)
                                                                                                                     aaCCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATAGGCTTTCAGGTTAGAGGCGTTGATTTGCATACGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTag                                  >  2:4702202/1‑150 (MQ=255)
                                                                                                                      aCCTATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTaga                                 >  1:4481896/1‑150 (MQ=255)
                                                                                                                            acatacaGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTg                           >  1:4304142/1‑150 (MQ=255)
                                                                                                                              atacaGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGc                         >  1:1046125/1‑150 (MQ=255)
                                                                                                                                  aGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACCTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGGTGATTTGCATCCGCGTACGTGCGGGTGGAATGCTCGGCGGAGAGTCCAGATTAGAGGCCTGGCAaga                     >  2:1978845/1‑150 (MQ=255)
                                                                                                                                   gCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAagag                    >  2:762267/1‑150 (MQ=255)
                                                                                                                                    ccTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAGt                   <  2:2613042/150‑1 (MQ=255)
                                                                                                                                     cTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAGTc                  >  2:2429681/1‑150 (MQ=255)
                                                                                                                                      tGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGAATAGAGGCCTGGCAAGAGTCg                 >  2:1768279/1‑150 (MQ=255)
                                                                                                                                       gACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAGTCGc                >  2:1440039/1‑150 (MQ=255)
                                                                                                                                        aCGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGTGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGCGAGTCCTGATTAGTGGCCTGGCACGAGTCGcc               >  1:1564061/1‑150 (MQ=255)
                                                                                                                                         cGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAGTCGCCa              >  1:412713/1‑150 (MQ=255)
                                                                                                                                          gATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAGTCGCCAc             >  1:258458/1‑150 (MQ=255)
                                                                                                                                            tAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAGTCGCCACTg           >  1:2053839/1‑150 (MQ=255)
                                                                                                                                               cagcCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAGTCGCCACTGACt        <  1:1981418/150‑1 (MQ=255)
                                                                                                                                               cagcCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAGTCGCCACTGACt        <  1:4000560/150‑1 (MQ=255)
                                                                                                                                                agcCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAGTCGCCACTGACtt       <  1:852551/150‑1 (MQ=255)
                                                                                                                                                agcCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGGTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCACGAGTCGCCACTGACtt       >  2:67818/1‑150 (MQ=255)
                                                                                                                                                  ccTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAGTCGCCACTGACTTCa     >  2:1189269/1‑150 (MQ=255)
                                                                                                                                                    tGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAGTCGCCACTGACTTCAcc   >  1:4661800/1‑150 (MQ=255)
                                                                                                                                                    tGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAGTCGCCACTGACTTCAcc   >  1:3773479/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCGCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAGTCGCCACTGACTTCACCG  >  CP009273/215987‑216285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A