Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 216,136 G→T P116Q (CCA→CAA)  rcsF ← putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273216,1360GT99.6% 740.1 / ‑5.0 220P116Q (CCA→CAA) rcsFputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (107/112);  minor base C (0/1);  total (107/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 220 total aligned reads displayed.

GCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAGTCGCCACTGACTT  >  CP009273/215989‑216280
                                                                                                                                                   |                                                                                                                                                
gcTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGc                                                                                                                                                <  1:911161/150‑1 (MQ=255)
gcTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGc                                                                                                                                                <  1:1912841/150‑1 (MQ=255)
   ttttACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTa                                                                                                                                             >  2:124971/1‑150 (MQ=255)
        ccAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCt                                                                                                                                        <  2:1779839/150‑1 (MQ=255)
         cAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTg                                                                                                                                       <  2:544739/150‑1 (MQ=255)
           gACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGt                                                                                                                                     >  2:1347032/1‑150 (MQ=255)
             ccTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGa                                                                                                                                   <  2:640845/150‑1 (MQ=255)
               tggctggcGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACt                                                                                                                                 >  1:1785279/1‑150 (MQ=255)
               tggctggcGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCACATTTCGCCGTAATGTTAAGCGCAGAAACGATACATACAGCCTGACGAGAGCAGCCTTGCGTACCGCTGGTGACt                                                                                                                                 <  1:1008253/150‑1 (MQ=255)
                 gctggcGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTc                                                                                                                               <  2:463845/150‑1 (MQ=255)
                        cgGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCTTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTa                                                                                                                        <  1:3705892/150‑1 (MQ=255)
                        cgGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTa                                                                                                                        <  1:3716067/150‑1 (MQ=255)
                        cgGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTa                                                                                                                        <  2:2057157/150‑1 (MQ=255)
                        cgGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTa                                                                                                                        >  2:2212404/1‑150 (MQ=255)
                         ggAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTAt                                                                                                                       >  2:218043/1‑150 (MQ=255)
                          gAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATg                                                                                                                      >  2:1522243/1‑150 (MQ=255)
                            aCGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCa                                                                                                                    <  1:1840703/150‑1 (MQ=255)
                                cGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTaa                                                                                                                >  2:318781/1‑150 (MQ=255)
                                 gAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAAt                                                                                                               >  1:1553570/1‑150 (MQ=255)
                                 gAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAAt                                                                                                               <  1:1417308/150‑1 (MQ=255)
                                  aaCTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATa                                                                                                              >  1:2021237/1‑150 (MQ=255)
                                   aCTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATAc                                                                                                             <  2:707436/150‑1 (MQ=255)
                                    cTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTAGAACTGGAAACTGCTCATTTCGCTGTTATGTTAAGCGCAGTACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACa                                                                                                            <  1:2624803/150‑1 (MQ=255)
                                       ttCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCa                                                                                                         <  1:2831887/150‑1 (MQ=255)
                                         cTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCAtt                                                                                                       <  1:1178756/150‑1 (MQ=255)
                                          tttATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTg                                                                                                      <  2:495614/150‑1 (MQ=255)
                                           ttATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTgg                                                                                                     <  2:2359891/150‑1 (MQ=255)
                                           ttATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTgg                                                                                                     >  1:2060903/1‑150 (MQ=255)
                                            tataGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGc                                                                                                    <  1:2544711/150‑1 (MQ=255)
                                              taGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCtt                                                                                                  <  2:1157879/150‑1 (MQ=255)
                                               aGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCttt                                                                                                 >  2:825138/1‑150 (MQ=255)
                                               aGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCttt                                                                                                 <  2:1575981/150‑1 (MQ=255)
                                                gggCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTc                                                                                                <  1:3573982/150‑1 (MQ=255)
                                                 ggCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCa                                                                                               >  1:198596/1‑150 (MQ=255)
                                                   cgagcgaATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCAtt                                                                                             <  2:2643082/150‑1 (MQ=255)
                                                    gagcgaATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCAttt                                                                                            >  1:3389098/1‑150 (MQ=255)
                                                      gcgaATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTa                                                                                          <  1:3586579/150‑1 (MQ=255)
                                                      gcgaATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTa                                                                                          <  1:651622/150‑1 (MQ=255)
                                                         aaTAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTagag                                                                                       <  2:2963593/150‑1 (MQ=255)
                                                          aTAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAgg                                                                                      >  2:3460507/1‑150 (MQ=255)
                                                           tAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGc                                                                                     >  2:76314/1‑150 (MQ=255)
                                                            aaCGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCg                                                                                    >  2:2559320/1‑150 (MQ=255)
                                                            aaCGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCg                                                                                    >  2:3042689/1‑150 (MQ=255)
                                                            aaCGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCg                                                                                    >  1:3513311/1‑150 (MQ=255)
                                                              cGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCTTTTTAGAGGCGtt                                                                                  <  2:2426091/150‑1 (MQ=255)
                                                               gCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTg                                                                                 >  1:3173896/1‑150 (MQ=255)
                                                                ccTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGa                                                                                >  2:2483040/1‑150 (MQ=255)
                                                                ccTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGa                                                                                >  1:2584527/1‑150 (MQ=255)
                                                                 cTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGAt                                                                               >  2:3230522/1‑150 (MQ=255)
                                                                 cTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGAt                                                                               <  1:2419626/150‑1 (MQ=255)
                                                                  tATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGAtt                                                                              <  2:1963310/150‑1 (MQ=255)
                                                                  tATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGAtt                                                                              >  2:767164/1‑150 (MQ=255)
                                                                   aTTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGAttt                                                                             <  1:958979/150‑1 (MQ=255)
                                                                   aTTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGAttt                                                                             >  1:2434866/1‑150 (MQ=255)
                                                                    tttGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTg                                                                            <  1:736644/150‑1 (MQ=255)
                                                                       gCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTACTACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCAt                                                                         >  2:3711390/1‑150 (MQ=255)
                                                                          cGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCg                                                                      >  2:1664713/1‑150 (MQ=255)
                                                                           gAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGc                                                                     >  2:226806/1‑150 (MQ=255)
                                                                            aaCTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCt                                                                    <  2:2035456/150‑1 (MQ=255)
                                                                            aaCTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCt                                                                    <  1:1136043/150‑1 (MQ=255)
                                                                              cTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTa                                                                  >  2:1185769/1‑150 (MQ=255)
                                                                                  aaaCTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTg                                                              >  2:299835/1‑150 (MQ=255)
                                                                                    aCTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCg                                                            <  1:3741461/150‑1 (MQ=255)
                                                                                    aCTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTCGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCg                                                            <  1:300365/150‑1 (MQ=255)
                                                                                         tCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTgg                                                       >  1:3085976/1‑150 (MQ=255)
                                                                                           aTTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGaa                                                     >  1:155788/1‑150 (MQ=255)
                                                                                           aTTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGaa                                                     >  2:565589/1‑150 (MQ=255)
                                                                                           aTTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGaa                                                     >  1:2521901/1‑150 (MQ=255)
                                                                                           aTTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGaa                                                     >  2:3290974/1‑150 (MQ=255)
                                                                                            tttCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAAt                                                    <  2:1307560/150‑1 (MQ=255)
                                                                                                gCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTc                                                >  2:3339685/1‑150 (MQ=255)
                                                                                                  cGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTcgg                                              <  2:1289742/150‑1 (MQ=255)
                                                                                                  cGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTcgg                                              >  2:3276924/1‑150 (MQ=255)
                                                                                                     aaTGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTcggcgg                                           >  2:913786/1‑150 (MQ=255)
                                                                                                     aaTGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATATTAGAGGCGTTGATTTGCATACGCTTACGTGCGGTTGGAATGCTcggcgg                                           >  1:2886029/1‑150 (MQ=255)
                                                                                                      aTGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGga                                          >  2:2486886/1‑150 (MQ=255)
                                                                                                       tGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGgag                                         <  2:1852619/150‑1 (MQ=255)
                                                                                                        gTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGgaga                                        <  1:553854/150‑1 (MQ=255)
                                                                                                            aGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTcc                                    <  2:2012288/150‑1 (MQ=255)
                                                                                                                 aGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGAtt                               <  2:3173896/150‑1 (MQ=255)
                                                                                                                   aaCCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTag                             <  1:3048194/150‑1 (MQ=255)
                                                                                                                   aaCCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTag                             >  1:710177/1‑150 (MQ=255)
                                                                                                                   aaCCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTag                             >  2:3441991/1‑150 (MQ=255)
                                                                                                                      cGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATTCAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAgg                          >  1:1911015/1‑150 (MQ=255)
                                                                                                                      cGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAgg                          >  1:3803469/1‑150 (MQ=255)
                                                                                                                        atacatacAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGcc                        <  2:2558789/150‑1 (MQ=255)
                                                                                                                         tacatacaGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCt                       <  1:3149494/150‑1 (MQ=255)
                                                                                                                          acatacaGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTg                      <  2:1396053/150‑1 (MQ=255)
                                                                                                                           catacaGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTgg                     <  1:1248181/150‑1 (MQ=255)
                                                                                                                            atacaGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGc                    >  1:2225288/1‑150 (MQ=255)
                                                                                                                               caGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAag                 >  2:231528/1‑150 (MQ=255)
                                                                                                                               caGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAag                 >  1:448700/1‑150 (MQ=255)
                                                                                                                               caGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAag                 >  1:381703/1‑150 (MQ=255)
                                                                                                                                        gATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAGTCGCCAc        >  2:1733308/1‑150 (MQ=255)
                                                                                                                                          tAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAGTCGCCACTg      >  1:1527269/1‑150 (MQ=255)
                                                                                                                                           agcagcCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAGTCGCCACTGa     <  2:3513311/150‑1 (MQ=255)
                                                                                                                                           agcagcCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAGTCGCCACTGa     <  1:2051338/150‑1 (MQ=255)
                                                                                                                                            gcagcCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAGTCGCCACTGAc    >  2:1027471/1‑150 (MQ=255)
                                                                                                                                              agcCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCACGAGTCGCCACTGACtt  >  2:3667958/1‑150 (MQ=255)
                                                                                                                                              agcCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAGTCGCCACTGACtt  >  1:2015169/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAGTCGCCACTGACTT  >  CP009273/215989‑216280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAGTCGCCACTGACTTCACCG  >  CP009273/215987‑216285
                                                                                                                                                     |                                                                                                                                                     
GCGCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTT                                                                                                                                                       <  SRR19973061.1527196/150‑1 (MQ=60)
  GCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGC                                                                                                                                                     <  SRR19973061.1916523/150‑1 (MQ=60)
  GCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGC                                                                                                                                                     <  SRR19973061.913499/150‑1 (MQ=60)
  GCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGC                                                                                                                                                     >  SRR19973061.3168251/1‑150 (MQ=60)
  GATTTTTACCAGATCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGC                                                                                                                                                     <  SRR19973061.1917571/150‑1 (MQ=60)
       TTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACC                                                                                                                                                >  SRR19973061.2040567/1‑150 (MQ=60)
         ACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGC                                                                                                                                              >  SRR19973061.1905262/1‑150 (MQ=60)
                 TGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACT                                                                                                                                      >  SRR19973061.2017335/1‑150 (MQ=60)
                 TGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACT                                                                                                                                      >  SRR19973061.1789752/1‑150 (MQ=60)
                 TGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCACATTTCGCCGTAATGTTAAGCGCAGAAACGATACATACAGCCTGACGAGAGCAGCCTTGCGTACCGCTGGTGACT                                                                                                                                      <  SRR19973061.1010822/150‑1 (MQ=60)
                    CTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCG                                                                                                                                   <  SRR19973061.417398/150‑1 (MQ=60)
                          CGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCTTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTA                                                                                                                             <  SRR19973061.3715366/150‑1 (MQ=60)
                          CGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTA                                                                                                                             <  SRR19973061.3725565/150‑1 (MQ=60)
                          CGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTA                                                                                                                             <  SRR19973061.3725564/150‑1 (MQ=60)
                             AACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGC                                                                                                                          >  SRR19973061.1928690/1‑150 (MQ=60)
                              ACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCA                                                                                                                         <  SRR19973061.1845311/150‑1 (MQ=60)
                                GGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGT                                                                                                                       >  SRR19973061.381968/1‑150 (MQ=60)
                                   GAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAAT                                                                                                                    <  SRR19973061.1420882/150‑1 (MQ=60)
                                   GAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAAT                                                                                                                    >  SRR19973061.1557429/1‑150 (MQ=60)
                                    AACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATA                                                                                                                   >  SRR19973061.1095656/1‑150 (MQ=60)
                                    AACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATA                                                                                                                   >  SRR19973061.2026314/1‑150 (MQ=60)
                                    AACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATA                                                                                                                   >  SRR19973061.844463/1‑150 (MQ=60)
                                      CTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTAGAACTGGAAACTGCTCATTTCGCTGTTATGTTAAGCGCAGTACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACA                                                                                                                 <  SRR19973061.2631549/150‑1 (MQ=60)
                                         TTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCA                                                                                                              <  SRR19973061.2839141/150‑1 (MQ=60)
                                           CTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTCTGCAGTAATACAGCATT                                                                                                            >  SRR19973061.3083171/1‑150 (MQ=60)
                                           CTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATT                                                                                                            <  SRR19973061.1181737/150‑1 (MQ=60)
                                             TTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGG                                                                                                          >  SRR19973061.2066089/1‑150 (MQ=60)
                                              TATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGC                                                                                                         <  SRR19973061.2551184/150‑1 (MQ=60)
                                                TAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTT                                                                                                       >  SRR19973061.1820201/1‑150 (MQ=60)
                                                TAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTT                                                                                                       <  SRR19973061.2332392/150‑1 (MQ=60)
                                                 AGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTT                                                                                                      <  SRR19973061.2085994/150‑1 (MQ=60)
                                                  GGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTC                                                                                                     <  SRR19973061.2677546/150‑1 (MQ=60)
                                                  GGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTC                                                                                                     <  SRR19973061.3583142/150‑1 (MQ=60)
                                                  GGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTC                                                                                                     <  SRR19973061.3584225/150‑1 (MQ=60)
                                                   GGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCA                                                                                                    >  SRR19973061.199187/1‑150 (MQ=60)
                                                      GAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTT                                                                                                 >  SRR19973061.448431/1‑150 (MQ=60)
                                                      GAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTT                                                                                                 >  SRR19973061.3397805/1‑150 (MQ=60)
                                                        GCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTA                                                                                               <  SRR19973061.653291/150‑1 (MQ=60)
                                                        GCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTA                                                                                               <  SRR19973061.3595772/150‑1 (MQ=60)
                                                           AATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAG                                                                                            <  SRR19973061.2063679/150‑1 (MQ=60)
                                                           AATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAG                                                                                            <  SRR19973061.147259/150‑1 (MQ=60)
                                                              AACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCG                                                                                         >  SRR19973061.3522304/1‑150 (MQ=60)
                                                              AACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCG                                                                                         <  SRR19973061.2867066/150‑1 (MQ=60)
                                                              AACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCG                                                                                         <  SRR19973061.1740981/150‑1 (MQ=60)
                                                                 GCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTG                                                                                      <  SRR19973061.689668/150‑1 (MQ=60)
                                                                 GCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTG                                                                                      >  SRR19973061.3182040/1‑150 (MQ=60)
                                                                  CCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGA                                                                                     >  SRR19973061.2591121/1‑150 (MQ=60)
                                                                  CCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGA                                                                                     >  SRR19973061.2591148/1‑150 (MQ=60)
                                                                   CTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGAT                                                                                    <  SRR19973061.514757/150‑1 (MQ=60)
                                                                   CTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGAT                                                                                    >  SRR19973061.3350330/1‑150 (MQ=60)
                                                                   CTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGAT                                                                                    <  SRR19973061.2425803/150‑1 (MQ=60)
                                                                     ATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTT                                                                                  >  SRR19973061.2441077/1‑150 (MQ=60)
                                                                     ATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTT                                                                                  <  SRR19973061.961396/150‑1 (MQ=60)
                                                                      TTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTG                                                                                 <  SRR19973061.738575/150‑1 (MQ=60)
                                                                              AACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCT                                                                         <  SRR19973061.1138937/150‑1 (MQ=60)
                                                                                CTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTA                                                                       <  SRR19973061.2428759/150‑1 (MQ=60)
                                                                                CTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTA                                                                       >  SRR19973061.2547469/1‑150 (MQ=60)
                                                                                   GAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGT                                                                    >  SRR19973061.3095394/1‑150 (MQ=60)
                                                                                      ACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCG                                                                 <  SRR19973061.827289/150‑1 (MQ=60)
                                                                                      ACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCG                                                                 <  SRR19973061.3751039/150‑1 (MQ=60)
                                                                                      ACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTCGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCG                                                                 <  SRR19973061.301163/150‑1 (MQ=60)
                                                                                        TGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGT                                                               <  SRR19973061.289316/150‑1 (MQ=60)
                                                                                          CTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTG                                                             <  SRR19973061.3407009/150‑1 (MQ=60)
                                                                                          CTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTG                                                             <  SRR19973061.1869693/150‑1 (MQ=60)
                                                                                           TCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGG                                                            >  SRR19973061.3093931/1‑150 (MQ=60)
                                                                                            CATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGA                                                           <  SRR19973061.632288/150‑1 (MQ=60)
                                                                                             ATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAA                                                          >  SRR19973061.156276/1‑150 (MQ=60)
                                                                                             ATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAA                                                          >  SRR19973061.156222/1‑150 (MQ=60)
                                                                                             ATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAA                                                          >  SRR19973061.2528321/1‑150 (MQ=60)
                                                                                                  GCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGATGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTC                                                     <  SRR19973061.1487926/150‑1 (MQ=60)
                                                                                                     GTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGC                                                  <  SRR19973061.3577373/150‑1 (MQ=60)
                                                                                                     GTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGC                                                  <  SRR19973061.3654007/150‑1 (MQ=60)
                                                                                                       AATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGG                                                >  SRR19973061.2894413/1‑150 (MQ=60)
                                                                                                       AATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATATTAGAGGCGTTGATTTGCATACGCTTACGTGCGGTTGGAATGCTCGGCGG                                                >  SRR19973061.2893428/1‑150 (MQ=60)
                                                                                                          GTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGA                                             <  SRR19973061.555311/150‑1 (MQ=60)
                                                                                                             AAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTC                                          >  SRR19973061.137574/1‑150 (MQ=60)
                                                                                                             AAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTC                                          <  SRR19973061.2022823/150‑1 (MQ=60)
                                                                                                                   AGAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATT                                    >  SRR19973061.3768545/1‑150 (MQ=60)
                                                                                                                    GAACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTA                                   <  SRR19973061.1192760/150‑1 (MQ=60)
                                                                                                                     AACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAG                                  <  SRR19973061.3056070/150‑1 (MQ=60)
                                                                                                                     AACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAG                                  >  SRR19973061.712042/1‑150 (MQ=60)
                                                                                                                     AACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAG                                  >  SRR19973061.712026/1‑150 (MQ=60)
                                                                                                                     AACCGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAG                                  <  SRR19973061.3056073/150‑1 (MQ=60)
                                                                                                                        CGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATTCAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGG                               >  SRR19973061.1915742/1‑150 (MQ=60)
                                                                                                                        CGATACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGG                               >  SRR19973061.3813161/1‑150 (MQ=60)
                                                                                                                           TACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCT                            <  SRR19973061.3157595/150‑1 (MQ=60)
                                                                                                                           TACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCT                            <  SRR19973061.3157594/150‑1 (MQ=60)
                                                                                                                            ACATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTG                           <  SRR19973061.3749249/150‑1 (MQ=60)
                                                                                                                             CATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACTGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGG                          <  SRR19973061.3605475/150‑1 (MQ=60)
                                                                                                                             CATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGG                          <  SRR19973061.1251275/150‑1 (MQ=60)
                                                                                                                              ATACAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGC                         >  SRR19973061.2230869/1‑150 (MQ=60)
                                                                                                                                 CAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAG                      <  SRR19973061.1166622/150‑1 (MQ=60)
                                                                                                                                 CAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAG                      >  SRR19973061.382745/1‑150 (MQ=60)
                                                                                                                                 CAGCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAG                      >  SRR19973061.449913/1‑150 (MQ=60)
                                                                                                                                   GCCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAG                    <  SRR19973061.928940/150‑1 (MQ=60)
                                                                                                                                    CCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAGT                   <  SRR19973061.2521950/150‑1 (MQ=60)
                                                                                                                                    CCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAGT                   <  SRR19973061.2521939/150‑1 (MQ=60)
                                                                                                                                    CCTGACGATAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAGT                   >  SRR19973061.1543985/1‑150 (MQ=60)
                                                                                                                                            TAGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAGTCGCCACTG           >  SRR19973061.1531083/1‑150 (MQ=60)
                                                                                                                                             AGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAGTCGCCACTGA          <  SRR19973061.2056498/150‑1 (MQ=60)
                                                                                                                                             AGCAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAGTCGCCACTGA          <  SRR19973061.2055714/150‑1 (MQ=60)
                                                                                                                                               CAGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAGTCGCCACTGACT        <  SRR19973061.2604298/150‑1 (MQ=60)
                                                                                                                                                AGCCTTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAGTCGCCACTGACTT       >  SRR19973061.2020230/1‑150 (MQ=60)
                                                                                                                                                    TTGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGGTTAGAGGCCTGGCAAGAGTCGCCACTGACTTCACC   <  SRR19973061.3622354/150‑1 (MQ=60)
                                                                                                                                                     TGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAGTCGCCACTGACTTCACCG  <  SRR19973061.542639/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GCGCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAGTCGCCACTGACTTCACCG  >  CP009273/215987‑216285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑