Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,228,033 C→A 42.9% A152S (GCT→TCT)  dsbB ← oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,228,0330CA42.9% 199.0 / 276.0 226A152S (GCT→TCT) dsbBoxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I
Reads supporting (aligned to +/- strand):  ref base C (61/68);  new base A (53/43);  total (114/112)
Fisher's exact test for biased strand distribution p-value = 2.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01

Only 100 of 228 total aligned reads displayed.

AGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTCCGGGAAACGAACCATAAAATCACAGGT  >  CP009273/1227884‑1228180
                                                                                                                                                     |                                                                                                                                                   
aGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGc                                                                                                                                                     >  2:261025/1‑150 (MQ=255)
  ttCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAgaga                                                                                                                                                   <  1:2516480/150‑1 (MQ=255)
   tCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGAt                                                                                                                                                  >  2:2849015/1‑150 (MQ=255)
   tCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGAGAt                                                                                                                                                  <  2:4302745/150‑1 (MQ=255)
    cTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGAGATa                                                                                                                                                 <  2:3513209/150‑1 (MQ=255)
       aTTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATaaaa                                                                                                                                              >  2:712575/1‑150 (MQ=255)
       aTTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGAGATaaaa                                                                                                                                              >  1:4515195/1‑150 (MQ=255)
       aTTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAAACACCACCAGCACTGCGACAATCAGGTAAGAGATaaaa                                                                                                                                              >  1:3755265/1‑150 (MQ=255)
          tACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATa                                                                                                                                           >  2:1457614/1‑150 (MQ=255)
           aCTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATAc                                                                                                                                          >  1:51900/1‑150 (MQ=255)
           aCTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATAc                                                                                                                                          <  1:3160818/150‑1 (MQ=255)
               aGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGag                                                                                                                                      <  2:4104540/150‑1 (MQ=255)
                gggTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGagc                                                                                                                                     <  1:1821176/150‑1 (MQ=255)
                gggTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGagc                                                                                                                                     <  1:1146073/150‑1 (MQ=255)
                    cAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGcc                                                                                                                                 >  1:1408913/1‑150 (MQ=255)
                    cAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGcc                                                                                                                                 >  1:1408852/1‑150 (MQ=255)
                     aaataaatATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCa                                                                                                                                <  2:2052859/150‑1 (MQ=255)
                      aataaataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAATAATACCGAGCAGCCAc                                                                                                                               >  2:3114528/1‑150 (MQ=255)
                      aataaataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCAc                                                                                                                               >  2:3114556/1‑150 (MQ=255)
                       ataaataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACt                                                                                                                              >  1:4522999/1‑150 (MQ=255)
                        taaataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTg                                                                                                                             >  1:2206260/1‑150 (MQ=255)
                         aaataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGc                                                                                                                            >  1:4364453/1‑150 (MQ=255)
                           atatAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCgg                                                                                                                          >  1:1939253/1‑150 (MQ=255)
                                gCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCAttt                                                                                                                     <  1:2951174/150‑1 (MQ=255)
                                 cGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTc                                                                                                                    >  1:4182827/1‑150 (MQ=255)
                                  ggCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTcc                                                                                                                   >  2:2480936/1‑150 (MQ=255)
                                  ggCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTcc                                                                                                                   <  2:533314/150‑1 (MQ=255)
                                  ggCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTcc                                                                                                                   <  2:2155840/150‑1 (MQ=255)
                                   gCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCa                                                                                                                  <  1:2712742/150‑1 (MQ=255)
                                   gCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCa                                                                                                                  >  2:2720092/1‑150 (MQ=255)
                                    cAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAg                                                                                                                 >  1:3365437/1‑150 (MQ=255)
                                       gAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGAcc                                                                                                              >  2:3687126/1‑150 (MQ=255)
                                         aaaaaGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTa                                                                                                            >  1:4152352/1‑150 (MQ=255)
                                           aaaGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTaaa                                                                                                          <  1:2098244/150‑1 (MQ=255)
                                           aaaGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTaaa                                                                                                          <  2:3667461/150‑1 (MQ=255)
                                              gcgcTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTaaaaaa                                                                                                       >  2:2711249/1‑150 (MQ=255)
                                                 cTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATcc                                                                                                    >  2:1661036/1‑150 (MQ=255)
                                                  tCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATccc                                                                                                   >  2:3817977/1‑150 (MQ=255)
                                                      gCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTg                                                                                               <  2:3435287/150‑1 (MQ=255)
                                                       cAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCACCCACCGCGGCATTTCCAGACCTAAAAAATCCCACTGa                                                                                              <  2:3340997/150‑1 (MQ=255)
                                                        aGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGAc                                                                                             <  1:1601481/150‑1 (MQ=255)
                                                          gAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGc                                                                                           >  2:1837252/1‑150 (MQ=255)
                                                            gcgcCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTc                                                                                         <  1:3144297/150‑1 (MQ=255)
                                                             cgcCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCg                                                                                        <  2:3055815/150‑1 (MQ=255)
                                                             cgcCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCg                                                                                        <  2:422062/150‑1 (MQ=255)
                                                               ccGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGgc                                                                                      <  1:3952850/150‑1 (MQ=255)
                                                                 gAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTACCAGACCTCACAAATCCCACTGACGCTCGgcgc                                                                                    >  2:2915545/1‑150 (MQ=255)
                                                                  aaTGGATTAGCTACCGAAAAGATCACTTTTTTTCGCTTTAAACTGCTGTGAAATCTCCACCAGCACTTCGACAATCATGTAAGCGATAAAAATTCCGAGCAGGCAGTGCGGCATTTCCAGACCTAATAAATACCTCTGAAGCTCGGCGCa                                                                                   <  2:2836954/150‑1 (MQ=255)
                                                                  aaTGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCa                                                                                   <  2:2836983/150‑1 (MQ=255)
                                                                  aaTGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCa                                                                                   >  2:475153/1‑150 (MQ=255)
                                                                     ggATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCATCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCTCTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATc                                                                                <  1:1024674/150‑1 (MQ=255)
                                                                      gATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCg                                                                               >  1:2347707/1‑150 (MQ=255)
                                                                       aTTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGc                                                                              >  1:174109/1‑150 (MQ=255)
                                                                             gACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAgg                                                                        <  2:2112672/150‑1 (MQ=255)
                                                                                 gAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGAc                                                                    >  1:2881832/1‑150 (MQ=255)
                                                                                 gAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGAc                                                                    >  2:1267979/1‑150 (MQ=255)
                                                                                 gAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGAc                                                                    >  2:1267950/1‑150 (MQ=255)
                                                                                  aaCAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACa                                                                   <  1:2128569/150‑1 (MQ=255)
                                                                                    cAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACaaa                                                                 >  1:3669664/1‑150 (MQ=255)
                                                                                    cAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACaaa                                                                 >  1:715179/1‑150 (MQ=255)
                                                                                         cACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACtt                                                            <  2:3931760/150‑1 (MQ=255)
                                                                                         cACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACtt                                                            <  2:3931811/150‑1 (MQ=255)
                                                                                          aCGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTg                                                           <  1:2711583/150‑1 (MQ=255)
                                                                                               ttttCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCa                                                      >  1:3426790/1‑150 (MQ=255)
                                                                                               ttttCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCa                                                      >  1:3426178/1‑150 (MQ=255)
                                                                                                tttCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCAc                                                     >  1:3741728/1‑150 (MQ=255)
                                                                                                    gCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCAc                                                 >  2:1426670/1‑150 (MQ=255)
                                                                                                      tttAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACtt                                               >  1:1903192/1‑150 (MQ=255)
                                                                                                           aCGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCa                                          <  1:1111864/150‑1 (MQ=255)
                                                                                                           aCGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCa                                          <  1:4444256/150‑1 (MQ=255)
                                                                                                            cGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCGCCCACTTATCCAg                                         >  1:1651569/1‑150 (MQ=255)
                                                                                                             ggCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGc                                        <  1:4165589/150‑1 (MQ=255)
                                                                                                              gCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGTCGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCg                                       <  1:261025/150‑1 (MQ=255)
                                                                                                              gCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCg                                       >  1:3081476/1‑150 (MQ=255)
                                                                                                              gCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCg                                       >  1:2401989/1‑150 (MQ=255)
                                                                                                                  ggAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAg                                   >  2:1671705/1‑150 (MQ=255)
                                                                                                                   gAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGc                                  >  1:1795212/1‑150 (MQ=255)
                                                                                                                   gAAATCACCACCAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGc                                  >  1:3507184/1‑150 (MQ=255)
                                                                                                                   gAAATCACCACCAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGc                                  >  1:1280197/1‑150 (MQ=255)
                                                                                                                     aaTCACCACCAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTCTCCAGCGGCAGCCa                                <  2:3173359/150‑1 (MQ=255)
                                                                                                                     aaTCACCACCAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCa                                <  2:3173341/150‑1 (MQ=255)
                                                                                                                       tCACCACCAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCAtt                              >  2:1061260/1‑150 (MQ=255)
                                                                                                                         accaccAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTcc                            <  2:646564/150‑1 (MQ=255)
                                                                                                                         accaccAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTcc                            <  1:2747714/150‑1 (MQ=255)
                                                                                                                         accaccAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTcc                            <  2:3834035/150‑1 (MQ=255)
                                                                                                                         accaccAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTcc                            <  2:3834042/150‑1 (MQ=255)
                                                                                                                          ccaccaGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTCCg                           <  1:2521173/150‑1 (MQ=255)
                                                                                                                              caGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTCCGGGaa                       <  1:1080600/150‑1 (MQ=255)
                                                                                                                              caGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTCCGGGaa                       >  1:612521/1‑150 (MQ=255)
                                                                                                                              caGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTCCGGGaa                       <  1:4102649/150‑1 (MQ=255)
                                                                                                                               aGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTCCGGGaaa                      >  1:2450415/1‑150 (MQ=255)
                                                                                                                               aGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTCCGGGaac                      >  2:2186661/1‑149 (MQ=255)
                                                                                                                                gCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTCCGGGAAAc                     <  2:695641/150‑1 (MQ=255)
                                                                                                                                   cTGCGACAATAAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTCCGGGAAACGaa                  <  1:3900908/150‑1 (MQ=255)
                                                                                                                                   cTGCGACAATAAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTCCGGGAAACGaa                  <  1:2386369/150‑1 (MQ=255)
                                                                                                                                      cGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTCCGGGAAACGAACCa               <  2:491542/150‑1 (MQ=255)
                                                                                                                                        aCAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTCCGGGAAACGAACCATa             >  2:2995558/1‑150 (MQ=255)
                                                                                                                                         cAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTCCGGGAAACGAACCATac            >  2:516788/1‑149 (MQ=255)
                                                                                                                                            tCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTCCGGGAAACGAACCATAAAAt         <  1:1678796/150‑1 (MQ=255)
                                                                                                                                                  aaGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTCCGGGAAACGAACCATAAAATCACAgg   <  2:3613281/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
AGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTCCGGGAAACGAACCATAAAATCACAGGT  >  CP009273/1227884‑1228180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A