Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F181 I0 R1 1262 323.1 10297870 97.5% 10040423 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,228,033 C→A 8.5% A152S (GCT→TCT)  dsbB ← oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,228,0330CA 8.5% 1137.1 / 52.3 308A152S (GCT→TCT) dsbBoxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I
Reads supporting (aligned to +/- strand):  ref base C (172/109);  new base A (14/13);  total (186/122)
Fisher's exact test for biased strand distribution p-value = 4.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 312 total aligned reads displayed.

AGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTCCGGGAAACGAACCATAAAATCACAGGTGG  >  CP009273/1227884‑1228182
                                                                                                                                                     |                                                                                                                                                     
     tGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACACTCAGGTAAGCGATaa                                                                                                                                                  >  1:4135476/1‑150 (MQ=255)
     tGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATaa                                                                                                                                                  >  2:35562/1‑150 (MQ=255)
     tGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGAGATaa                                                                                                                                                  >  2:1527007/1‑150 (MQ=255)
       aTTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATaaaa                                                                                                                                                >  1:2359645/1‑150 (MQ=255)
        tgtACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATaaaaa                                                                                                                                               <  2:2108968/148‑1 (MQ=255)
         ttACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAAt                                                                                                                                              <  2:481824/150‑1 (MQ=255)
         ttACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAAt                                                                                                                                              <  2:4195578/150‑1 (MQ=255)
          tACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATa                                                                                                                                             >  2:4755100/1‑150 (MQ=255)
          tACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATa                                                                                                                                             >  2:4384434/1‑150 (MQ=255)
           aCTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATAc                                                                                                                                            >  1:4221336/1‑150 (MQ=255)
           aCTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGAGATAAAAATAc                                                                                                                                            >  2:2725802/1‑150 (MQ=255)
            cTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATAcc                                                                                                                                           <  1:15097/150‑1 (MQ=255)
            cTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATAcc                                                                                                                                           <  1:4759769/150‑1 (MQ=255)
             tGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCg                                                                                                                                          >  1:593669/1‑150 (MQ=255)
              gAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAAt                                                                                                                                              <  2:736062/145‑1 (MQ=255)
              gAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGa                                                                                                                                         >  2:105812/1‑150 (MQ=255)
               aGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGag                                                                                                                                        <  1:354591/150‑1 (MQ=255)
                 ggTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGagca                                                                                                                                      >  1:1182311/1‑150 (MQ=255)
                     aaataaatATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCa                                                                                                                                  >  1:3120254/1‑150 (MQ=255)
                     aaataaatATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCa                                                                                                                                  >  2:4130737/1‑150 (MQ=255)
                         aaataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGc                                                                                                                              <  2:2153253/150‑1 (MQ=255)
                           atatAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTACGCGATAAAAATACCGAGCAGCCACTGCgg                                                                                                                            >  1:4589333/1‑150 (MQ=255)
                                 cGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTc                                                                                                                      <  1:2798551/150‑1 (MQ=255)
                                 cGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTc                                                                                                                      >  2:4673421/1‑150 (MQ=255)
                                  ggCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTcc                                                                                                                     >  2:3380021/1‑150 (MQ=255)
                                      ggAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGAc                                                                                                                 <  1:429541/150‑1 (MQ=255)
                                        aaaaaaGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCt                                                                                                               <  2:3197412/150‑1 (MQ=255)
                                         aaaaaGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTa                                                                                                              >  2:2477827/1‑150 (MQ=255)
                                              gcgcTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTaaaaaa                                                                                                         >  1:3736694/1‑150 (MQ=255)
                                                  tCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACATAAAAAATccc                                                                                                     >  2:2680064/1‑150 (MQ=255)
                                                    ccGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCAc                                                                                                   <  1:3312631/150‑1 (MQ=255)
                                                    ccGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCAc                                                                                                   >  1:2930149/1‑150 (MQ=255)
                                                     cGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACt                                                                                                  <  1:803517/150‑1 (MQ=255)
                                                           aGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCt                                                                                            <  2:3433351/150‑1 (MQ=255)
                                                              gcCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCgg                                                                                         >  2:3153272/1‑150 (MQ=255)
                                                                 gAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGgcgc                                                                                      >  2:4341384/1‑150 (MQ=255)
                                                                 gAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGgcgc                                                                                      >  1:5063854/1‑150 (MQ=255)
                                                                 gAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGgcgc                                                                                      >  2:711079/1‑150 (MQ=255)
                                                                  aaTGGATTAGCGACCGAACAGATCACGTTTTGTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCa                                                                                     <  2:5093234/150‑1 (MQ=255)
                                                                   aTGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCaa                                                                                    >  1:1022434/1‑150 (MQ=255)
                                                                    tGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAAt                                                                                   >  2:1209467/1‑150 (MQ=255)
                                                                     ggATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATc                                                                                  <  1:1451725/150‑1 (MQ=255)
                                                                     ggATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATc                                                                                  <  2:1933276/150‑1 (MQ=255)
                                                                       aTTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGc                                                                                >  1:1645404/1‑150 (MQ=255)
                                                                         tAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCa                                                                              >  2:2210056/1‑150 (MQ=255)
                                                                         tAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAAGACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCa                                                                              <  1:1381607/150‑1 (MQ=255)
                                                                           gCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCaga                                                                            <  2:2762070/150‑1 (MQ=255)
                                                                            cGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCagag                                                                           <  1:3226697/150‑1 (MQ=255)
                                                                            cGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCagag                                                                           <  1:165280/150‑1 (MQ=255)
                                                                             gACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAgg                                                                          >  2:2950965/1‑150 (MQ=255)
                                                                             gACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAgg                                                                          <  1:2548294/150‑1 (MQ=255)
                                                                             gACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAgg                                                                          >  2:1913575/1‑150 (MQ=255)
                                                                                cGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGa                                                                       >  1:339095/1‑150 (MQ=255)
                                                                                 gAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGAc                                                                      >  2:462976/1‑150 (MQ=255)
                                                                                 gAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGAc                                                                      >  2:786635/1‑150 (MQ=255)
                                                                                 gAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGAc                                                                      >  2:1409452/1‑150 (MQ=255)
                                                                                 gAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGAc                                                                      >  2:4092035/1‑150 (MQ=255)
                                                                                   aCAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACaa                                                                    >  2:2834629/1‑150 (MQ=255)
                                                                                     aGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAac                                                                  >  1:2184661/1‑150 (MQ=255)
                                                                                     aGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAac                                                                  >  2:2046567/1‑150 (MQ=255)
                                                                                       aTCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGAGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAacac                                                                <  2:2330486/150‑1 (MQ=255)
                                                                                        tCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACt                                                               <  1:3389947/150‑1 (MQ=255)
                                                                                         cACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACtt                                                              >  2:3079724/1‑150 (MQ=255)
                                                                                                 ttCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCAcc                                                      >  2:14401/1‑150 (MQ=255)
                                                                                                 ttCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCAcc                                                      >  1:3102630/1‑150 (MQ=255)
                                                                                                    gCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCAc                                                   <  1:2626729/150‑1 (MQ=255)
                                                                                                    gCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCAc                                                   >  1:4826057/1‑150 (MQ=255)
                                                                                                       ttAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTa                                                >  2:3651789/1‑150 (MQ=255)
                                                                                                        tAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTAt                                               <  1:4341374/150‑1 (MQ=255)
                                                                                                        tAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTAt                                               >  1:1980729/1‑150 (MQ=255)
                                                                                                          aaCGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATcc                                             >  1:5013278/1‑150 (MQ=255)
                                                                                                            cGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAg                                           <  2:4970974/150‑1 (MQ=255)
                                                                                                             ggCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGc                                          <  1:1175985/150‑1 (MQ=255)
                                                                                                             ggCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGc                                          >  1:3132685/1‑150 (MQ=255)
                                                                                                               cTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCgg                                        >  1:274801/1‑150 (MQ=255)
                                                                                                                tGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGc                                       >  1:3005700/1‑150 (MQ=255)
                                                                                                                tGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGc                                       <  2:1237383/150‑1 (MQ=255)
                                                                                                                   gAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGc                                    <  2:3823767/150‑1 (MQ=255)
                                                                                                                   gAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGc                                    <  2:1252045/150‑1 (MQ=255)
                                                                                                                    aaaTCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGcc                                   >  1:636799/1‑150 (MQ=255)
                                                                                                                    aaaTCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGcc                                   >  2:4968215/1‑150 (MQ=255)
                                                                                                                     aaTCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCa                                  <  2:16826/150‑1 (MQ=255)
                                                                                                                          ccaccaGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTCCg                             <  1:3992602/150‑1 (MQ=255)
                                                                                                                          ccaccaGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTCCg                             <  2:4507735/150‑1 (MQ=255)
                                                                                                                           caccaGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTCCgg                            >  1:2344720/1‑150 (MQ=255)
                                                                                                                            accaGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTCCggg                           >  1:4524922/1‑150 (MQ=255)
                                                                                                                            accaGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTCCggg                           >  1:2225850/1‑150 (MQ=255)
                                                                                                                             ccaGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTCCGGGa                          <  1:1578799/150‑1 (MQ=255)
                                                                                                                                 cACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTCCGGGAAACg                      <  1:2313494/150‑1 (MQ=255)
                                                                                                                                   cTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTCCGGGAAACGaa                    >  1:559521/1‑150 (MQ=255)
                                                                                                                                       gACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTCCGGGAAACGAACCAt                >  1:307077/1‑150 (MQ=255)
                                                                                                                                         cAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTCCGGGAAACGAACCATaa              <  2:2881816/150‑1 (MQ=255)
                                                                                                                                          aaTCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTCCGGGAAACGAACCATaaa             <  1:1305277/150‑1 (MQ=255)
                                                                                                                                            tCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTCCGGGAAACGAACCATAAAAt           >  1:3666113/1‑150 (MQ=255)
                                                                                                                                               ggTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTCCGGGAAACGAACCATAAAATcac        >  2:449654/1‑150 (MQ=255)
                                                                                                                                                 tAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTCCGGGAAACGAACCATAAAATCACAg      >  1:1852048/1‑150 (MQ=255)
                                                                                                                                                    gCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTCCGGGAAACGAACCATAAAATCACAGGTg   <  2:4221336/150‑1 (MQ=255)
                                                                                                                                                    gCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTCCGGGAAACGAACCATAAAATCACAGGTg   <  1:3829905/150‑1 (MQ=255)
                                                                                                                                                    gCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTCCGGGAAACGAACCATAAAATCACAGGTg   >  2:666969/1‑150 (MQ=255)
                                                                                                                                                     cGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTCCGGGAAACGAACCATAAAATCACAGGTgg  <  1:3966243/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACTGACGCTCGGCGCAATCGCCAGAGGCGACAAACACTTGCGGCACCCACTTATCCAGCGGCAGCCATTCCGGGAAACGAACCATAAAATCACAGGTGG  >  CP009273/1227884‑1228182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A