Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,984,987 C→A 69.5% T38T (ACG→ACT)  yecA ← UPF0149 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,984,9870CA69.5% 235.5 / 221.5 213T38T (ACG→ACT) yecAUPF0149 family protein
Reads supporting (aligned to +/- strand):  ref base C (30/35);  new base A (70/78);  total (100/113)
Fisher's exact test for biased strand distribution p-value = 8.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 216 total aligned reads displayed.

TGCGGTATCGGCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTCGCCAGCACCT  >  CP009273/1984840‑1985136
                                                                                                                                                   |                                                                                                                                                     
     tATCGGCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACTGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATa                                                                                                                                                <  2:3734029/150‑1 (MQ=255)
     tATCGGCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATa                                                                                                                                                >  2:3750460/1‑150 (MQ=255)
      aTCGGCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATaa                                                                                                                                               >  2:1353293/1‑150 (MQ=255)
      aTCGGCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATaa                                                                                                                                               >  2:1352833/1‑150 (MQ=255)
        cGGCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAAc                                                                                                                                             >  2:3217742/1‑150 (MQ=255)
         ggCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAAcc                                                                                                                                            >  1:4354815/1‑150 (MQ=255)
         ggCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAAcc                                                                                                                                            >  2:1993145/1‑150 (MQ=255)
         ggCCATATGTTGAAAAGCCAGATTCAGAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGTCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAAcc                                                                                                                                            <  2:4321588/150‑1 (MQ=255)
            cATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTc                                                                                                                                         >  1:4454607/1‑150 (MQ=255)
            cATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTc                                                                                                                                         >  1:3601157/1‑150 (MQ=255)
             atatGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTcc                                                                                                                                        <  2:3821351/150‑1 (MQ=255)
              tatGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCa                                                                                                                                       >  2:1537712/1‑150 (MQ=255)
              tatGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCa                                                                                                                                       <  2:3754759/150‑1 (MQ=255)
                  ttGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTc                                                                                                                                   <  2:1662563/150‑1 (MQ=255)
                  ttGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTc                                                                                                                                   <  1:2902558/150‑1 (MQ=255)
                        aGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCcac                                                                                                                             >  2:1380295/1‑150 (MQ=255)
                        aGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCcac                                                                                                                             >  1:1334160/1‑150 (MQ=255)
                            aGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCTATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCa                                                                                                                         <  2:1331359/150‑1 (MQ=255)
                            aGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCa                                                                                                                         <  2:4296230/150‑1 (MQ=255)
                               ttCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAgg                                                                                                                      >  1:1687819/1‑150 (MQ=255)
                                 cATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGAt                                                                                                                    <  1:1727668/150‑1 (MQ=255)
                                  aTAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATg                                                                                                                   >  1:496711/1‑150 (MQ=255)
                                        cgcgTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTgg                                                                                                             <  1:2104741/150‑1 (MQ=255)
                                          cgTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTc                                                                                                           >  2:3926474/1‑150 (MQ=255)
                                           gTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCa                                                                                                          >  1:1944474/1‑150 (MQ=255)
                                           gTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCa                                                                                                          <  1:859564/150‑1 (MQ=255)
                                           gTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCa                                                                                                          >  1:4112258/1‑150 (MQ=255)
                                             cATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAgt                                                                                                        >  2:873335/1‑150 (MQ=255)
                                                  ctTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGtgttgt                                                                                                   <  2:3153061/150‑1 (MQ=255)
                                                   tttCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCCCACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTa                                                                                                  <  2:287277/150‑1 (MQ=255)
                                                   tttCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACCCGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTa                                                                                                  <  2:2465094/150‑1 (MQ=255)
                                                   tttCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTa                                                                                                  <  2:1415653/150‑1 (MQ=255)
                                                   tttCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTa                                                                                                  <  2:285656/150‑1 (MQ=255)
                                                    ttCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTAt                                                                                                 <  2:1470420/150‑1 (MQ=255)
                                                    ttCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTAt                                                                                                 <  1:3842681/150‑1 (MQ=255)
                                                       tctGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTg                                                                                              <  1:1515301/150‑1 (MQ=255)
                                                             gCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGa                                                                                        >  1:2217670/1‑150 (MQ=255)
                                                              cccAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGaa                                                                                       >  1:1284108/1‑150 (MQ=255)
                                                               ccAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAat                                                                                      >  2:3024092/1‑150 (MQ=255)
                                                                 aGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAatat                                                                                    <  2:514529/150‑1 (MQ=255)
                                                                  gcgcGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATc                                                                                   <  1:1635868/150‑1 (MQ=255)
                                                                  gcgcGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATc                                                                                   <  1:3572712/150‑1 (MQ=255)
                                                                    gcgGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGt                                                                                 <  1:1608908/150‑1 (MQ=255)
                                                                      ggCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTcc                                                                               <  1:158449/150‑1 (MQ=255)
                                                                      ggCAAATAGTCAGCCCCACCCAACACTGAAACCAGCCACTGTTCAGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTcc                                                                               >  2:2901135/1‑150 (MQ=255)
                                                                        cacaTAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAg                                                                             <  2:831884/150‑1 (MQ=255)
                                                                            tAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCAt                                                                         <  1:667997/150‑1 (MQ=255)
                                                                             aGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCAtt                                                                        >  2:2342881/1‑150 (MQ=255)
                                                                             aGTCAGCCCCACCCCACACGGCAACCAGCCACTGGTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATCAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCAtt                                                                        <  1:2825951/150‑1 (MQ=255)
                                                                                  gCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAAc                                                                   <  1:1566109/150‑1 (MQ=255)
                                                                                  gCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAAc                                                                   <  1:1566289/150‑1 (MQ=255)
                                                                                   ccccaccacaCACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACt                                                                  <  2:3424917/150‑1 (MQ=255)
                                                                                     ccaccccacACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCa                                                                <  1:4008499/150‑1 (MQ=255)
                                                                                     ccaccccacACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCa                                                                >  1:3757897/1‑150 (MQ=255)
                                                                                     ccaccccacACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCa                                                                <  1:231009/150‑1 (MQ=255)
                                                                                      caccccacACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCAc                                                               >  2:2320935/1‑150 (MQ=255)
                                                                                      caccccacACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCAc                                                               <  1:998628/150‑1 (MQ=255)
                                                                                        ccccacACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACtt                                                             >  1:3899080/1‑150 (MQ=255)
                                                                                         cccacACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACttt                                                            <  2:3888042/150‑1 (MQ=255)
                                                                                            acacGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGt                                                         >  1:568627/1‑150 (MQ=255)
                                                                                             cacGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGtt                                                        >  1:4020058/1‑150 (MQ=255)
                                                                                             cacGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGtt                                                        <  2:3067669/150‑1 (MQ=255)
                                                                                              acGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGttt                                                       >  1:3431259/1‑150 (MQ=255)
                                                                                               cGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTa                                                      <  2:562292/150‑1 (MQ=255)
                                                                                                ggCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTaa                                                     >  2:2563696/1‑150 (MQ=255)
                                                                                                  cAACCAGCCACTGTTCCGGTTCAGTCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACg                                                   >  1:3798593/1‑150 (MQ=255)
                                                                                                   aaCCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACgg                                                  >  2:4373115/1‑150 (MQ=255)
                                                                                                     ccAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTc                                                <  1:3305966/150‑1 (MQ=255)
                                                                                                      cAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTcc                                               >  1:1618757/1‑150 (MQ=255)
                                                                                                       aGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATCAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTccc                                              <  1:2133191/150‑1 (MQ=255)
                                                                                                       aGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTccc                                              <  1:2133080/150‑1 (MQ=255)
                                                                                                       aGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTccc                                              >  2:1991181/1‑150 (MQ=255)
                                                                                                            cTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAACCCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGtttt                                         <  1:779603/150‑1 (MQ=255)
                                                                                                              gTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCa                                       >  1:2372832/1‑150 (MQ=255)
                                                                                                              gTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCa                                       >  1:2372954/1‑150 (MQ=255)
                                                                                                               ttCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCAt                                      >  2:2514401/1‑150 (MQ=255)
                                                                                                                tCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATa                                     <  2:903196/150‑1 (MQ=255)
                                                                                                                tCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATa                                     <  2:1860139/150‑1 (MQ=255)
                                                                                                                     ttCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCtt                                >  1:3221162/1‑150 (MQ=255)
                                                                                                                     ttCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCtt                                <  1:3591165/150‑1 (MQ=255)
                                                                                                                     ttCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCtt                                <  1:3591142/150‑1 (MQ=255)
                                                                                                                     ttCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCtt                                >  2:2160298/1‑150 (MQ=255)
                                                                                                                       cAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCtttt                              <  1:3089078/150‑1 (MQ=255)
                                                                                                                        aaTCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTc                             <  2:4112258/150‑1 (MQ=255)
                                                                                                                          tctcTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCt                           <  1:566990/150‑1 (MQ=255)
                                                                                                                          tctcTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCt                           <  1:3138656/150‑1 (MQ=255)
                                                                                                                          tctcTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCt                           <  1:4094737/150‑1 (MQ=255)
                                                                                                                             ctTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTtgt                        >  1:1760390/1‑150 (MQ=255)
                                                                                                                             ctTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTtgt                        >  1:2225609/1‑150 (MQ=255)
                                                                                                                             cgtGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTtgt                        <  2:1688481/148‑1 (MQ=255)
                                                                                                                                   gagaACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGAtct                  >  1:4540270/1‑150 (MQ=255)
                                                                                                                                   gagaACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACAACAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGAtct                  >  1:3923062/1‑150 (MQ=255)
                                                                                                                                      aaCTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCaa               <  1:4334395/150‑1 (MQ=255)
                                                                                                                                       aCTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAAc              <  1:267424/150‑1 (MQ=255)
                                                                                                                                         tCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTc            >  1:889536/1‑150 (MQ=255)
                                                                                                                                         tCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTc            <  2:3303238/150‑1 (MQ=255)
                                                                                                                                             cacCGCAGTCATTAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTCGCCa        <  1:3024089/150‑1 (MQ=255)
                                                                                                                                             cacCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTCGCCa        >  1:2905486/1‑150 (MQ=255)
                                                                                                                                                cgccgTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGGTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTCGCCAGCa     >  2:4303996/1‑150 (MQ=255)
                                                                                                                                                   cgTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTCGCCAGCACCt  <  2:1944474/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TGCGGTATCGGCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTCGCCAGCACCT  >  CP009273/1984840‑1985136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCGGTATCGGCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTCGCCAGCACCT  >  CP009273/1984840‑1985136
                                                                                                                                                   |                                                                                                                                                     
TGCGGTATCGGCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGT                                                                                                                                                     <  SRR19973076.46362/150‑1 (MQ=60)
  CGGTATCGGCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCA                                                                                                                                                   >  SRR19973076.1368737/1‑150 (MQ=60)
    GTATCGGCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGAGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAAT                                                                                                                                                 <  SRR19973076.2908913/150‑1 (MQ=60)
      ATCGGCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAA                                                                                                                                               >  SRR19973076.398714/1‑150 (MQ=60)
         GGCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACC                                                                                                                                            <  SRR19973076.4074867/150‑1 (MQ=60)
         GGCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAAAAAACC                                                                                                                                            >  SRR19973076.4361234/1‑150 (MQ=60)
          GCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCG                                                                                                                                           >  SRR19973076.8066/1‑150 (MQ=60)
            CATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTC                                                                                                                                         >  SRR19973076.3606383/1‑150 (MQ=60)
            CATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTC                                                                                                                                         >  SRR19973076.3606389/1‑150 (MQ=60)
            CATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTC                                                                                                                                         >  SRR19973076.4461239/1‑150 (MQ=60)
             ATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCC                                                                                                                                        >  SRR19973076.190637/1‑150 (MQ=60)
              TATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCA                                                                                                                                       <  SRR19973076.523388/150‑1 (MQ=60)
                  TTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTC                                                                                                                                   <  SRR19973076.2906770/150‑1 (MQ=60)
                   TGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCC                                                                                                                                  >  SRR19973076.1620446/1‑150 (MQ=60)
                   TGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCC                                                                                                                                  >  SRR19973076.1621071/1‑150 (MQ=60)
                   TGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCC                                                                                                                                  >  SRR19973076.1621162/1‑150 (MQ=60)
                       AAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCA                                                                                                                              >  SRR19973076.896343/1‑150 (MQ=60)
                        AGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCAC                                                                                                                             >  SRR19973076.1336121/1‑150 (MQ=60)
                            AGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCA                                                                                                                         <  SRR19973076.2134313/150‑1 (MQ=60)
                               TTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGG                                                                                                                      >  SRR19973076.1690227/1‑150 (MQ=60)
                               TTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGG                                                                                                                      >  SRR19973076.1690288/1‑150 (MQ=60)
                                 CATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGAT                                                                                                                    <  SRR19973076.3254875/150‑1 (MQ=60)
                                 CATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGAT                                                                                                                    <  SRR19973076.1729945/150‑1 (MQ=60)
                                 CATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGAT                                                                                                                    <  SRR19973076.1730175/150‑1 (MQ=60)
                                  ATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATG                                                                                                                   >  SRR19973076.497508/1‑150 (MQ=60)
                                        CGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGG                                                                                                             <  SRR19973076.4101335/150‑1 (MQ=60)
                                        CGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGG                                                                                                             <  SRR19973076.2107798/150‑1 (MQ=60)
                                        CGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGG                                                                                                             <  SRR19973076.2106607/150‑1 (MQ=60)
                                        CGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGG                                                                                                             >  SRR19973076.3920522/1‑150 (MQ=60)
                                           GTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCA                                                                                                          >  SRR19973076.1947298/1‑150 (MQ=60)
                                           GTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCA                                                                                                          <  SRR19973076.860831/150‑1 (MQ=60)
                                           GTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCA                                                                                                          >  SRR19973076.4118333/1‑150 (MQ=60)
                                                 TCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTG                                                                                                    >  SRR19973076.4500870/1‑150 (MQ=60)
                                                    TTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTAT                                                                                                 <  SRR19973076.3848372/150‑1 (MQ=60)
                                                    TTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTAT                                                                                                 <  SRR19973076.3848335/150‑1 (MQ=60)
                                                       TCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTG                                                                                              <  SRR19973076.3779562/150‑1 (MQ=60)
                                                       TCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTG                                                                                              <  SRR19973076.1517526/150‑1 (MQ=60)
                                                         TGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGT                                                                                            >  SRR19973076.712645/1‑150 (MQ=60)
                                                             GCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGA                                                                                        >  SRR19973076.2220723/1‑150 (MQ=60)
                                                             GCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGA                                                                                        >  SRR19973076.2220864/1‑150 (MQ=60)
                                                             GCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGA                                                                                        >  SRR19973076.2220879/1‑150 (MQ=60)
                                                              CCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAA                                                                                       >  SRR19973076.3206154/1‑150 (MQ=60)
                                                              CCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAA                                                                                       >  SRR19973076.1286011/1‑150 (MQ=60)
                                                                  GCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATC                                                                                   <  SRR19973076.1638261/150‑1 (MQ=60)
                                                                  GCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATC                                                                                   <  SRR19973076.3578002/150‑1 (MQ=60)
                                                                  GCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATC                                                                                   <  SRR19973076.3577929/150‑1 (MQ=60)
                                                                  GCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATC                                                                                   <  SRR19973076.3577903/150‑1 (MQ=60)
                                                                  GCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATC                                                                                   <  SRR19973076.3577290/150‑1 (MQ=60)
                                                                   CGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCG                                                                                  <  SRR19973076.2776894/150‑1 (MQ=60)
                                                                    GCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGT                                                                                 <  SRR19973076.1610441/150‑1 (MQ=60)
                                                                    GCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGT                                                                                 <  SRR19973076.2097403/150‑1 (MQ=60)
                                                                    GCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGT                                                                                 <  SRR19973076.1611260/150‑1 (MQ=60)
                                                                      GGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCC                                                                               <  SRR19973076.158722/150‑1 (MQ=60)
                                                                            TAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCAT                                                                         <  SRR19973076.669003/150‑1 (MQ=60)
                                                                             AGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATT                                                                        <  SRR19973076.3004249/150‑1 (MQ=60)
                                                                             AGTCAGCCCCACCCCACACGGCAACCAGCCACTGGTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATCAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATT                                                                        <  SRR19973076.2830038/150‑1 (MQ=60)
                                                                                  GCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAAC                                                                   <  SRR19973076.1568405/150‑1 (MQ=60)
                                                                                  GCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAAC                                                                   <  SRR19973076.1568432/150‑1 (MQ=60)
                                                                                  GCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAAC                                                                   <  SRR19973076.1568585/150‑1 (MQ=60)
                                                                                  GCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAAC                                                                   <  SRR19973076.1568679/150‑1 (MQ=60)
                                                                                     CCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCA                                                                >  SRR19973076.3763397/1‑150 (MQ=60)
                                                                                     CCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCA                                                                >  SRR19973076.2881401/1‑150 (MQ=60)
                                                                                     CCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCA                                                                <  SRR19973076.231405/150‑1 (MQ=60)
                                                                                     CCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCA                                                                <  SRR19973076.4014446/150‑1 (MQ=60)
                                                                                     CCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCA                                                                <  SRR19973076.4014421/150‑1 (MQ=60)
                                                                                      CACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCAC                                                               <  SRR19973076.1000111/150‑1 (MQ=60)
                                                                                       ACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGACCAGCCATTCCAACTCACT                                                              >  SRR19973076.4428955/1‑150 (MQ=60)
                                                                                        CCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTT                                                             >  SRR19973076.3904842/1‑150 (MQ=60)
                                                                                        CCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTT                                                             >  SRR19973076.3904836/1‑150 (MQ=60)
                                                                                          CCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTC                                                           >  SRR19973076.889826/1‑150 (MQ=60)
                                                                                            ACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGT                                                         >  SRR19973076.569584/1‑150 (MQ=60)
                                                                                            ACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGT                                                         >  SRR19973076.569516/1‑150 (MQ=60)
                                                                                            ACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGT                                                         >  SRR19973076.569166/1‑150 (MQ=60)
                                                                                            ACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGT                                                         >  SRR19973076.569414/1‑150 (MQ=60)
                                                                                             CACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTT                                                        >  SRR19973076.4026000/1‑150 (MQ=60)
                                                                                              ACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTT                                                       <  SRR19973076.2302334/150‑1 (MQ=60)
                                                                                              ACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTT                                                       >  SRR19973076.3436232/1‑150 (MQ=60)
                                                                                                  CAACCAGCCACTGTTCCGGTTCAGTCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACG                                                   >  SRR19973076.3804168/1‑150 (MQ=60)
                                                                                                  CAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACG                                                   <  SRR19973076.1554307/150‑1 (MQ=60)
                                                                                                  CAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACG                                                   >  SRR19973076.3804408/1‑150 (MQ=60)
                                                                                                     CCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTC                                                >  SRR19973076.2364981/1‑150 (MQ=60)
                                                                                                     CCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTC                                                <  SRR19973076.3310765/150‑1 (MQ=60)
                                                                                                     CCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTC                                                <  SRR19973076.3311090/150‑1 (MQ=60)
                                                                                                      CAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCC                                               >  SRR19973076.1621128/1‑150 (MQ=60)
                                                                                                       AGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATCAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCC                                              <  SRR19973076.2136285/150‑1 (MQ=60)
                                                                                                       AGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCC                                              <  SRR19973076.2136221/150‑1 (MQ=60)
                                                                                                       AGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCC                                              <  SRR19973076.2136174/150‑1 (MQ=60)
                                                                                                            CTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAACCCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTT                                         <  SRR19973076.780756/150‑1 (MQ=60)
                                                                                                              GTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCA                                       >  SRR19973076.2376244/1‑150 (MQ=60)
                                                                                                              GTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCA                                       >  SRR19973076.2376293/1‑150 (MQ=60)
                                                                                                              GTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCA                                       >  SRR19973076.2376366/1‑150 (MQ=60)
                                                                                                              GTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCA                                       >  SRR19973076.2376369/1‑150 (MQ=60)
                                                                                                                     TTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTT                                >  SRR19973076.3225839/1‑150 (MQ=60)
                                                                                                                     TTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTT                                <  SRR19973076.3596381/150‑1 (MQ=60)
                                                                                                                     TTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTT                                <  SRR19973076.3596358/150‑1 (MQ=60)
                                                                                                                     TTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTT                                <  SRR19973076.3596359/150‑1 (MQ=60)
                                                                                                                      TCAAACTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTT                               <  SRR19973076.4352630/150‑1 (MQ=60)
                                                                                                                       CAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTT                              <  SRR19973076.3093562/150‑1 (MQ=60)
                                                                                                                          TCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCT                           <  SRR19973076.4100779/150‑1 (MQ=60)
                                                                                                                          TCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCT                           <  SRR19973076.567879/150‑1 (MQ=60)
                                                                                                                          TCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCT                           <  SRR19973076.3143171/150‑1 (MQ=60)
                                                                                                                          TCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCT                           <  SRR19973076.3143210/150‑1 (MQ=60)
                                                                                                                             CTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGT                        >  SRR19973076.2228812/1‑150 (MQ=60)
                                                                                                                             CTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGT                        >  SRR19973076.1762937/1‑150 (MQ=60)
                                                                                                                             CTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGT                        >  SRR19973076.1761495/1‑150 (MQ=60)
                                                                                                                                  GGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATC                   <  SRR19973076.2372999/150‑1 (MQ=60)
                                                                                                                                   GAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCT                  >  SRR19973076.4547004/1‑150 (MQ=60)
                                                                                                                                   GAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCT                  <  SRR19973076.1305706/150‑1 (MQ=60)
                                                                                                                                   GAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACAACAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCT                  >  SRR19973076.3928859/1‑150 (MQ=60)
                                                                                                                                     GAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCA                >  SRR19973076.4225432/1‑150 (MQ=60)
                                                                                                                                     GAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCA                >  SRR19973076.4225391/1‑150 (MQ=60)
                                                                                                                                      AACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAA               <  SRR19973076.4340798/150‑1 (MQ=60)
                                                                                                                                       ACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAAC              <  SRR19973076.267866/150‑1 (MQ=60)
                                                                                                                                         TCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTC            >  SRR19973076.3721915/1‑150 (MQ=60)
                                                                                                                                         TCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTC            >  SRR19973076.890856/1‑150 (MQ=60)
                                                                                                                                             CACCGCAGTCATTAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTCGCCA        <  SRR19973076.3028487/150‑1 (MQ=60)
                                                                                                                                             CACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTCGCCA        >  SRR19973076.2909700/1‑150 (MQ=60)
                                                                                                                                             CACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTCGCCA        <  SRR19973076.3028490/150‑1 (MQ=60)
                                                                                                                                             CACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTAAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTCGCCA        >  SRR19973076.2911089/1‑150 (MQ=60)
                                                                                                                                                   AGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTCGCCAGCACCT  >  SRR19973076.3252784/1‑150 (MQ=60)
                                                                                                                                                   AGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTCGCCAGCACCT  >  SRR19973076.3252837/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
TGCGGTATCGGCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTCGCCAGCACCT  >  CP009273/1984840‑1985136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑