Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,329,729 G→T 56.2% A132E (GCG→GAG)  yfaA ← DUF2138 family protein, putative host defense protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,329,7290GT56.2% 19.9 / inf 254A132E (GCG→GAG) yfaADUF2138 family protein, putative host defense protein
Reads supporting (aligned to +/- strand):  ref base G (56/55);  new base T (79/63);  total (136/118)
Fisher's exact test for biased strand distribution p-value = 4.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 259 total aligned reads displayed.

TATTTATCTTGATGCTACTGATTTCCGTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGATAATAGAAAACGAAATCTTCGCTCAGAACA  >  CP009273/2329580‑2329878
                                                                                                                                                     |                                                                                                                                                     
tATTTATCTTGATGCTACTGATTTCCGTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCATCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAgcg                                                                                                                                                       >  1:4012596/1‑150 (MQ=255)
     aTCTTGATGCTACTGATTTCCGTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTctgcc                                                                                                                                                  >  1:2858940/1‑150 (MQ=255)
     aTCTTGATGCTACTGATTTCCGTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGctgcc                                                                                                                                                  >  1:1001131/1‑150 (MQ=255)
      tCTTGATGCTACTGATTTCCGTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTctgcct                                                                                                                                                 <  2:3171707/150‑1 (MQ=255)
       cTTGATGCTACTGATTTCCGTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTctgcctg                                                                                                                                                >  2:3978319/1‑150 (MQ=255)
            tGCTACTGATTTCCGTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCgg                                                                                                                                           <  2:1208485/150‑1 (MQ=255)
                  tGATTTCCGTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGAt                                                                                                                                     <  2:2041307/150‑1 (MQ=255)
                  tGATTTCCGTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGAt                                                                                                                                     >  2:2647587/1‑150 (MQ=255)
                  tGATTTCCGTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGcgcg                                                                                                                                            >  1:3619205/1‑143 (MQ=255)
                  tGATTTCCGTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGcgcg                                                                                                                                            <  2:3619205/143‑1 (MQ=255)
                     tttCCGTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTa                                                                                                                                  <  2:1001131/150‑1 (MQ=255)
                     tttCCGTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTa                                                                                                                                  <  2:2721195/150‑1 (MQ=255)
                      ttCCGTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTaa                                                                                                                                 >  1:4279115/1‑150 (MQ=255)
                           ttttGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTg                                                                                                                            >  1:3714348/1‑150 (MQ=255)
                                cTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGaa                                                                                                                       <  1:2876573/150‑1 (MQ=255)
                                 ttAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGaaa                                                                                                                      <  2:2257119/150‑1 (MQ=255)
                                  tAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGaaaa                                                                                                                     >  2:1325777/1‑150 (MQ=255)
                                  tAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGaaaa                                                                                                                     >  1:1843754/1‑150 (MQ=255)
                                   aaCTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAag                                                                                                                    >  1:703881/1‑150 (MQ=255)
                                      tGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGc                                                                                                                 >  2:2927835/1‑150 (MQ=255)
                                          tGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTAt                                                                                                             >  2:2125348/1‑150 (MQ=255)
                                          tGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTAt                                                                                                             <  2:3465944/150‑1 (MQ=255)
                                              gCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCttt                                                                                                         >  2:62103/1‑150 (MQ=255)
                                              gCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCttt                                                                                                         <  2:580904/150‑1 (MQ=255)
                                              gCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCAGATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCttt                                                                                                         >  1:4408808/1‑150 (MQ=255)
                                               cTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTc                                                                                                        >  1:4193768/1‑150 (MQ=255)
                                               cTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTc                                                                                                        <  2:1755258/150‑1 (MQ=255)
                                                tGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCa                                                                                                       <  1:196741/150‑1 (MQ=255)
                                                  gTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGc                                                                                                     >  1:3465130/1‑150 (MQ=255)
                                                      cGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTga                                                                                                 >  1:1874011/1‑150 (MQ=255)
                                                          aaaCAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATc                                                                                             <  2:4282800/150‑1 (MQ=255)
                                                          aaaCAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATc                                                                                             >  2:3526186/1‑150 (MQ=255)
                                                          aaaCAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATc                                                                                             >  2:3526905/1‑150 (MQ=255)
                                                           aacaacAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCg                                                                                            <  1:3496724/150‑1 (MQ=255)
                                                           aacaacAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCg                                                                                            <  2:2027803/150‑1 (MQ=255)
                                                           aacaacAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCg                                                                                            <  2:2027287/150‑1 (MQ=255)
                                                              aacaaTGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCgtgt                                                                                         >  2:891886/1‑150 (MQ=255)
                                                                 aaTGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCa                                                                                      <  1:4424198/150‑1 (MQ=255)
                                                                  aTGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCAt                                                                                     <  2:2512928/150‑1 (MQ=255)
                                                                  aTGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCAt                                                                                     >  2:959673/1‑150 (MQ=255)
                                                                   tGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATa                                                                                    <  2:1964899/150‑1 (MQ=255)
                                                                   tGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATa                                                                                    >  2:2991718/1‑150 (MQ=255)
                                                                    ggCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAg                                                                                   >  2:1336087/1‑150 (MQ=255)
                                                                      cTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGAc                                                                                 >  2:2919417/1‑150 (MQ=255)
                                                                        ccAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACaa                                                                               <  1:867850/150‑1 (MQ=255)
                                                                          agcagTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATa                                                                             >  2:3944133/1‑150 (MQ=255)
                                                                          agcagTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATa                                                                             <  1:2919417/150‑1 (MQ=255)
                                                                          agcagTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATa                                                                             >  2:3944646/1‑150 (MQ=255)
                                                                            cagTTTGCTTAAACCACTGCGCTGGATGAGCACCATATAATGTGAAAGATGGCATTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATacg                                                                           <  2:3149147/150‑1 (MQ=255)
                                                                            cagTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATacg                                                                           <  1:665343/150‑1 (MQ=255)
                                                                              gTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATacgac                                                                         <  2:2083070/150‑1 (MQ=255)
                                                                                  gCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAAt                                                                     <  1:1511633/150‑1 (MQ=255)
                                                                                   cTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAAtg                                                                    >  2:1358581/1‑150 (MQ=255)
                                                                                        aCCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGcc                                                               >  1:276200/1‑150 (MQ=255)
                                                                                         ccACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCt                                                              <  2:3827625/150‑1 (MQ=255)
                                                                                          cACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCtt                                                             >  1:2100243/1‑150 (MQ=255)
                                                                                             tGCGCTGGATCTGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCaa                                                          <  2:1291778/150‑1 (MQ=255)
                                                                                               cgcTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATg                                                        >  1:4325054/1‑150 (MQ=255)
                                                                                                gcTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGc                                                       <  2:1621426/150‑1 (MQ=255)
                                                                                                gcTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGc                                                       <  2:1090714/150‑1 (MQ=255)
                                                                                                  tGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGccc                                                     >  1:1157985/1‑150 (MQ=255)
                                                                                                  tGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGccc                                                     >  1:3673483/1‑150 (MQ=255)
                                                                                                  tGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGccc                                                     >  1:4022825/1‑150 (MQ=255)
                                                                                                   ggATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCa                                                    >  1:3939523/1‑150 (MQ=255)
                                                                                                   ggATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCa                                                    >  1:1181286/1‑150 (MQ=255)
                                                                                                    gATCAGCACCATATAATGTGAAAGATGGGCTTGTTTATCGTGCGACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAg                                                   <  2:4236243/150‑1 (MQ=255)
                                                                                                    gATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAg                                                   <  1:3044793/150‑1 (MQ=255)
                                                                                                       cAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACg                                                <  2:4402607/150‑1 (MQ=255)
                                                                                                        aGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGa                                               >  1:1289499/1‑150 (MQ=255)
                                                                                                         gCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGAt                                              >  1:1701843/1‑150 (MQ=255)
                                                                                                           aCCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCg                                            >  2:38144/1‑150 (MQ=255)
                                                                                                           aCCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCg                                            >  1:1569991/1‑150 (MQ=255)
                                                                                                              atatAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACTAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCa                                         <  2:1709110/150‑1 (MQ=255)
                                                                                                               tataATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCAt                                        <  2:2418379/150‑1 (MQ=255)
                                                                                                               tataATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCAt                                        <  1:4539012/150‑1 (MQ=255)
                                                                                                                  aaTGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAATAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGAt                                     <  2:3704666/150‑1 (MQ=255)
                                                                                                                  aaTGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGAt                                     <  1:2048385/150‑1 (MQ=255)
                                                                                                                  aaTGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGAt                                     >  2:1185211/1‑150 (MQ=255)
                                                                                                                   aTGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGAtt                                    <  1:1080682/150‑1 (MQ=255)
                                                                                                                        aaaGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGat                               >  2:4472741/1‑150 (MQ=255)
                                                                                                                         aaGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGata                              <  1:2191862/150‑1 (MQ=255)
                                                                                                                          aGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGataa                             <  2:2857964/150‑1 (MQ=255)
                                                                                                                           gATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGataat                            <  2:750652/150‑1 (MQ=255)
                                                                                                                           gATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGataat                            >  1:1038481/1‑150 (MQ=255)
                                                                                                                              ggCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGATAATAGa                         <  2:42070/150‑1 (MQ=255)
                                                                                                                               gCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGATAATAGaa                        >  2:3856022/1‑150 (MQ=255)
                                                                                                                               gCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGATAATAGaa                        >  2:1109738/1‑150 (MQ=255)
                                                                                                                                   tGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGATAATAGAAAACg                    <  2:726900/150‑1 (MQ=255)
                                                                                                                                     tttATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGATAATAGAAAACGaa                  >  1:3443498/1‑150 (MQ=255)
                                                                                                                                      ttATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGATAATAGAAAACGaaa                 <  1:404902/150‑1 (MQ=255)
                                                                                                                                       tATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCTTAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGATAATAGAAAACGAAAt                <  2:1857107/150‑1 (MQ=255)
                                                                                                                                       tATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGATAATAGAAAACGAAAt                >  1:324107/1‑150 (MQ=255)
                                                                                                                                        aTCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGATAATAGAAAACGAAATc               <  1:3878722/150‑1 (MQ=255)
                                                                                                                                         tCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGATAATAGAAAACGAAATCt              <  2:519514/150‑1 (MQ=255)
                                                                                                                                          cGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGATAATAGAAAACGAAATCtt             <  1:4111284/150‑1 (MQ=255)
                                                                                                                                           gTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGATAATAGAAAACGAAATCTTc            <  1:4251991/150‑1 (MQ=255)
                                                                                                                                            tGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGATAATAGAAAACGAAATCTTCg           <  1:3349291/150‑1 (MQ=255)
                                                                                                                                            tGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGATAATAGAAAACGAAATCTTCg           >  2:2365350/1‑150 (MQ=255)
                                                                                                                                                 caGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGATAATAGAAAACGAAATCTTCGCTCAg      >  2:1945713/1‑150 (MQ=255)
                                                                                                                                                     gcTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGATAATAGAAAACGAAATCTTCGCTCAGAACa  >  1:2579713/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TATTTATCTTGATGCTACTGATTTCCGTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGATAATAGAAAACGAAATCTTCGCTCAGAACA  >  CP009273/2329580‑2329878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A