Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,329,729 G→T 18.9% A132E (GCG→GAG)  yfaA ← DUF2138 family protein, putative host defense protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,329,7290GT18.9% 743.8 / 114.0 254A132E (GCG→GAG) yfaADUF2138 family protein, putative host defense protein
Reads supporting (aligned to +/- strand):  ref base G (109/95);  new base T (28/20);  total (139/115)
Fisher's exact test for biased strand distribution p-value = 6.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 260 total aligned reads displayed.

TATTTATCTTGATGCTACTGATTTCCGTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGATAATAGAAAACGAAATCTTCGCTCAGAAC  >  CP009273/2329580‑2329877
                                                                                                                                                     |                                                                                                                                                    
tATTTATCTTGATGCTACTGATTTCCGTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAgcg                                                                                                                                                      >  2:1789759/1‑150 (MQ=255)
tATTTATCTTGATGCTACTCATTTCCGTTTTGCTTAACTGTCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGATTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCATGTTTATCGTGCCACAgcg                                                                                                                                                      >  2:2239392/1‑150 (MQ=255)
  tttATCTTGATGCTACTGATTTCCGTTTTGCTTAACTGGCTTTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGct                                                                                                                                                    >  1:3709611/1‑150 (MQ=255)
  tttATCTTGATGCTACTGATTTCCGTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGct                                                                                                                                                    >  2:483754/1‑150 (MQ=255)
  tttATCTTGATGCTACTGATTTCCGTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGct                                                                                                                                                    <  2:1560240/150‑1 (MQ=255)
  tttATCTTGATGCTACTGATTTCCGTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGct                                                                                                                                                    <  2:3827034/150‑1 (MQ=255)
  tttATCTTGATGCTACTGATTTCCGTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGATTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGct                                                                                                                                                    >  1:2120831/1‑150 (MQ=255)
       cTTGATGCTACTGATTTCCGTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGctgcctg                                                                                                                                               >  1:2570314/1‑150 (MQ=255)
        ttGATGCTACTGATTTCCGTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTctgcctgc                                                                                                                                              <  2:2556118/150‑1 (MQ=255)
        ttGATGCTACTGATTTCCGTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGctgcctgc                                                                                                                                              >  2:3292308/1‑150 (MQ=255)
         tGATGCTACTGATTTCCGTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTgcg                                                                                                                                             <  2:2669944/150‑1 (MQ=255)
           aTGCTACTGATTTCCGTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGcgcg                                                                                                                                           >  1:1227164/1‑150 (MQ=255)
           aTGCTACTGATTTCCGTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCAGATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGcgcg                                                                                                                                           >  1:4091472/1‑150 (MQ=255)
                aCTGATTTCCGTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTg                                                                                                                                      >  1:3167018/1‑150 (MQ=255)
                aCTGATTTCCGTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTg                                                                                                                                      >  2:1768395/1‑150 (MQ=255)
                aCTGATTTCCGTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTg                                                                                                                                      <  2:4366112/150‑1 (MQ=255)
                aCTGATTTCCGTTTTGCTTAAATGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCATGTTTATCGAGCCACAGCTCTGCCTGCGCGGGCTg                                                                                                                                      >  1:85039/1‑150 (MQ=255)
                  tGATTTCCGTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGAt                                                                                                                                    <  2:2569457/150‑1 (MQ=255)
                   gATTTCCGTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATc                                                                                                                                   <  1:36732/150‑1 (MQ=255)
                     tttCCGTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTa                                                                                                                                 >  1:1345442/1‑150 (MQ=255)
                      ttCCGTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTaa                                                                                                                                >  1:2205648/1‑150 (MQ=255)
                        ccGTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAaga                                                                                                                              <  2:242159/150‑1 (MQ=255)
                        ccGTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATAAAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAaga                                                                                                                              >  1:2734258/1‑150 (MQ=255)
                        ccGTTTTGCTTAACTGGCCGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAaga                                                                                                                              >  1:3075134/1‑150 (MQ=255)
                         cGTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAagag                                                                                                                             >  2:3989036/1‑150 (MQ=255)
                          gTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGt                                                                                                                            >  2:2246071/1‑150 (MQ=255)
                          gTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGt                                                                                                                            <  2:1054901/150‑1 (MQ=255)
                            tttGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGa                                                                                                                          <  1:2964383/150‑1 (MQ=255)
                              tGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACg                                                                                                                        >  2:2121190/1‑150 (MQ=255)
                               gCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGa                                                                                                                       >  2:2961079/1‑150 (MQ=255)
                                 ttAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGaaa                                                                                                                     <  2:4540101/150‑1 (MQ=255)
                                  tAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGaaaa                                                                                                                    >  1:1084532/1‑150 (MQ=255)
                                  tAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGaaaa                                                                                                                    >  2:4549722/1‑150 (MQ=255)
                                  tAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGaaaa                                                                                                                    >  1:457562/1‑150 (MQ=255)
                                     cTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAagag                                                                                                                 >  1:3851995/1‑150 (MQ=255)
                                        gCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCtt                                                                                                              <  2:1977949/150‑1 (MQ=255)
                                        gCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCtt                                                                                                              <  2:1671053/150‑1 (MQ=255)
                                         cTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTa                                                                                                             <  2:1736339/150‑1 (MQ=255)
                                            tCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCt                                                                                                          >  1:2993706/1‑150 (MQ=255)
                                              gCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCttt                                                                                                        >  1:2839771/1‑150 (MQ=255)
                                                tGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCa                                                                                                      >  2:3060366/1‑150 (MQ=255)
                                                tGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCa                                                                                                      <  1:833724/150‑1 (MQ=255)
                                                      cGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTga                                                                                                <  2:1970177/150‑1 (MQ=255)
                                                        gCAAACAACAATGGCTCCAGCAGTTTGCTTACACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTgaga                                                                                              <  2:533753/150‑1 (MQ=255)
                                                         cAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACTGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGAt                                                                                             <  1:3943180/150‑1 (MQ=255)
                                                          aaaCAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATc                                                                                            <  1:1948640/150‑1 (MQ=255)
                                                           aacaacAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCg                                                                                           >  2:3606075/1‑150 (MQ=255)
                                                            acaacaATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCgt                                                                                          <  2:4124045/150‑1 (MQ=255)
                                                             caacaaTGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCgtg                                                                                         <  2:3190237/150‑1 (MQ=255)
                                                                   tGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATa                                                                                   >  2:1492196/1‑150 (MQ=255)
                                                                      cTCCAGCAGTTTTCTTAAACCACTGCGCTGGATCAGCACCATATAAAGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTTACGAAAAGAGCTGATCTTTAAGAGTGAGATCGTGTTCATATAc                                                                                >  2:1899696/1‑150 (MQ=255)
                                                                       tCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACa                                                                               >  2:2519013/1‑150 (MQ=255)
                                                                       tCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACa                                                                               >  1:1468715/1‑150 (MQ=255)
                                                                        ccAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCACTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACaa                                                                              >  2:865948/1‑150 (MQ=255)
                                                                        ccAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCACTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACaa                                                                              >  2:866223/1‑150 (MQ=255)
                                                                          agcagTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATa                                                                            >  2:2049662/1‑150 (MQ=255)
                                                                           gcagTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATac                                                                           <  1:3198584/150‑1 (MQ=255)
                                                                            cagTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATacg                                                                          >  1:332702/1‑150 (MQ=255)
                                                                             agTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATacga                                                                         >  1:3436263/1‑150 (MQ=255)
                                                                                     tAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAAtgct                                                                 <  2:1213420/150‑1 (MQ=255)
                                                                                      aaaCCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAAtgctg                                                                >  1:968850/1‑150 (MQ=255)
                                                                                        aCCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGcc                                                              <  2:1130070/150‑1 (MQ=255)
                                                                                        aCCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGcc                                                              >  1:1204209/1‑150 (MQ=255)
                                                                                         ccACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCt                                                             <  1:1110041/150‑1 (MQ=255)
                                                                                             tGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCaa                                                         <  1:3789374/150‑1 (MQ=255)
                                                                                              gcgcTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAAt                                                        <  2:424409/150‑1 (MQ=255)
                                                                                               cgcTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATg                                                       <  2:2542992/150‑1 (MQ=255)
                                                                                               cgcTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATg                                                       >  1:2095106/1‑150 (MQ=255)
                                                                                                 cTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGcc                                                     >  1:4264189/1‑150 (MQ=255)
                                                                                                        aGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACTAATGCTGCCTTCAATTCCCAGACGa                                              >  1:730608/1‑150 (MQ=255)
                                                                                                          caacATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATc                                            <  2:1698676/147‑1 (MQ=255)
                                                                                                           aCCATATAATGTGAAAGATGGCATTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCg                                           >  2:121663/1‑150 (MQ=255)
                                                                                                             cATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGc                                         <  1:4346306/150‑1 (MQ=255)
                                                                                                                ataATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATg                                      <  2:486989/150‑1 (MQ=255)
                                                                                                                ataATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATg                                      <  2:4040107/150‑1 (MQ=255)
                                                                                                                   aTGTGAAAGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGAtt                                   <  2:1685813/150‑1 (MQ=255)
                                                                                                                        aaaGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGTGTGACGAATAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGat                              <  1:2547364/150‑1 (MQ=255)
                                                                                                                        aaaGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGat                              <  2:662259/150‑1 (MQ=255)
                                                                                                                         aaGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGata                             <  2:536552/150‑1 (MQ=255)
                                                                                                                          aGATGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGataa                            >  1:2742017/1‑150 (MQ=255)
                                                                                                                          aGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGataa                            >  1:3091205/1‑150 (MQ=255)
                                                                                                                            aTGGCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGataata                          <  2:709852/150‑1 (MQ=255)
                                                                                                                             tGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGATAATAg                         <  2:1574501/150‑1 (MQ=255)
                                                                                                                             tGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGATAATAg                         >  1:467291/1‑150 (MQ=255)
                                                                                                                              ggCCTTGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGATAATAGa                        >  1:419067/1‑150 (MQ=255)
                                                                                                                                 cTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGATAATAGaaaa                     <  2:956141/150‑1 (MQ=255)
                                                                                                                                  ttGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGATAATAGAAAAc                    <  2:3312033/150‑1 (MQ=255)
                                                                                                                                  ttGTTTATCGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGATAATAGAAAAc                    >  1:456516/1‑150 (MQ=255)
                                                                                                                                    gTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGATAATAGAAAACGa                  >  2:4603143/1‑150 (MQ=255)
                                                                                                                                      ttATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGATAATAGAAAACGaaa                <  2:5997/150‑1 (MQ=255)
                                                                                                                                       tATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGATAATAGAAAACGAAAt               >  2:2992000/1‑150 (MQ=255)
                                                                                                                                       tATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTAAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGATAATAGAAAACGAAAt               >  1:2821275/1‑150 (MQ=255)
                                                                                                                                          cGTGCCACAGCTCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGATAATAGAAAACGAAATCtt            <  2:2903070/150‑1 (MQ=255)
                                                                                                                                          cGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGATAATAGAAAACGAAATCtt            >  2:1511843/1‑150 (MQ=255)
                                                                                                                                            tGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGATAATAGAAAACGAAATCTTCg          >  1:574942/1‑150 (MQ=255)
                                                                                                                                              ccACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGATAATAGAAAACGAAATCTTCGCt        <  2:347236/150‑1 (MQ=255)
                                                                                                                                                  aGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGATAATAGAAAACGAAATCTTCGCTCAGa    >  1:2318148/1‑150 (MQ=255)
                                                                                                                                                  aGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGATAATAGAAAACGAAATCTTCGCTCAGa    >  1:2082558/1‑150 (MQ=255)
                                                                                                                                                  aGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGATAATAGAAAACGAAATCTTCGCTCAGa    >  2:4216000/1‑150 (MQ=255)
                                                                                                                                                   gcgcTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGATAATAGAAAACGAAATCTTCGCTCAGaa   >  2:3635446/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TATTTATCTTGATGCTACTGATTTCCGTTTTGCTTAACTGGCTGTCGCTGGTAGCGGCAAACAACAATGGCTCCAGCAGTTTGCTTAAACCACTGCGCTGGATCAGCACCATATAATGTGAAAGATGGCCTTGTTTATCGTGCCACAGCGCTGCCTGCGCGGGCTGATCTAAGAGTGACGAAAAGAGCTTATCTTTCAGCGTGAGATCGTGTTCATAGACAATACGACGAATGCTGCCTTCAATGCCCAGACGATCGGCATGATTCTGATAATAGAAAACGAAATCTTCGCTCAGAAC  >  CP009273/2329580‑2329877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A