Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F202 I0 R1	1397	275.7	9089454	95.2%	8653160	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,370,361:1	+G	100%	coding (711/759 nt)	menH ←	2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,370,361	1	.	G	95.6%	1040.3 / 39.1	272	coding (711/759 nt)	menH	2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase
Reads supporting (aligned to +/- strand): ref base . (6/6); new base G (134/126); total (140/132)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 288 total aligned reads displayed.

TTTTTCATAACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTT‑TCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATC  >  CP009273/2370212‑2370504

  tttCATAACTAATGTCCTCGAAACCTTCGGAGCAGTCGTTCCATTAATCCGGTGCGTAATTGATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGACTCGCTAGATATGAGGCAGACTTGCGATTACGCCAGCGGGAtttgt                                                                                                                                                <  1:4368274/150‑3 (MQ=255)

  tttCATAACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGAtttgt                                                                                                                                                >  2:3337462/1‑148 (MQ=255)

  tttCATAACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGAtttgt                                                                                                                                                >  1:4021431/1‑148 (MQ=255)

   ttCATAACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGAtttgtc                                                                                                                                               >  2:1787274/1‑147 (MQ=255)

     cATACCGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGAtttgtccc                                                                                                                                             <  1:4391413/150‑6 (MQ=255)

          cGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATgc                                                                                                                                        >  2:1571185/1‑150 (MQ=255)

          cGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATgc                                                                                                                                        <  1:853338/150‑1 (MQ=255)

           gAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATgcg                                                                                                                                       >  1:4376235/1‑150 (MQ=255)

             aTGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGcgcg                                                                                                                                     <  1:151265/150‑1 (MQ=255)

              tGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGt                                                                                                                                    >  1:3779358/1‑150 (MQ=255)

                 ccTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTAt                                                                                                                                 <  2:2485140/150‑1 (MQ=255)

                 ccTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTAt                                                                                                                                 <  2:2445607/150‑1 (MQ=255)

                  cTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATg                                                                                                                                >  2:3161134/1‑150 (MQ=255)

                  cTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATg                                                                                                                                >  2:4124682/1‑150 (MQ=255)

                   tCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGt                                                                                                                               >  1:96359/1‑150 (MQ=255)

                     gAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTcc                                                                                                                             <  2:3900826/150‑1 (MQ=255)

                     gAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTcc                                                                                                                             <  1:3000040/150‑1 (MQ=255)

                       aaCCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCAgg                                                                                                                           <  2:1859398/150‑1 (MQ=255)

                          cTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGgcgc                                                                                                                        >  2:893184/1‑150 (MQ=255)

                            tCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGa                                                                                                                      <  2:916422/150‑1 (MQ=255)

                              ggAGCAGTGGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCACGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTCCCAATACGCCAGCGGGATTTGTCGCGATGCGCGTTATGTCCGGCGCGAgg                                                                                                                    >  2:4407135/1‑150 (MQ=255)

                              ggAGCAGTCGAGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCAAATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGAGCGTTATGTCCGGCGCGAgg                                                                                                                    >  2:3608101/1‑150 (MQ=255)

                                aGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGaa                                                                                                                  <  1:1097142/150‑1 (MQ=255)

                                 gCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTT‑TCCCGATGCGCGTTATGTCCGGCGCGAGGAATg                                                                                                                <  2:470990/150‑1 (MQ=255)

                                  cAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATg                                                                                                                >  2:645635/1‑150 (MQ=255)

                                    gTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGAc                                                                                                              <  1:3354441/150‑1 (MQ=255)

                                        tGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATgg                                                                                                          >  2:3419644/1‑150 (MQ=255)

                                         gCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGc                                                                                                         >  2:784788/1‑150 (MQ=255)

                                         gCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGc                                                                                                         <  2:4494586/150‑1 (MQ=255)

                                         gCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGc                                                                                                         <  2:4494420/150‑1 (MQ=255)

                                            aTTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGt                                                                                                      >  2:3014154/1‑150 (MQ=255)

                                             ttCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTc                                                                                                     <  2:2064281/150‑1 (MQ=255)

                                              tCAACCGGTGCGTAAAGCATTGCTTCATCATGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACTTGGCTGTCg                                                                                                    <  1:3515314/150‑1 (MQ=255)

                                              tCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCg                                                                                                    <  1:3515286/150‑1 (MQ=255)

                                               cAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCgg                                                                                                   <  2:438130/150‑1 (MQ=255)

                                               cAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCgg                                                                                                   <  2:229887/150‑1 (MQ=255)

                                                      tGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAg                                                                                            >  2:2889462/1‑150 (MQ=255)

                                                       gCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGt                                                                                           <  1:2943875/150‑1 (MQ=255)

                                                           aaaGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTccg                                                                                       >  2:816032/1‑150 (MQ=255)

                                                             aGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTccgcc                                                                                     <  2:2921850/150‑1 (MQ=255)

                                                             aGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTccgcc                                                                                     >  1:2595254/1‑150 (MQ=255)

                                                                aTTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCgccgcc                                                                                  >  2:35635/1‑150 (MQ=255)

                                                                    cTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAggg                                                                              <  1:1698814/150‑1 (MQ=255)

                                                                     ttCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTT‑TCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGgcg                                                                            >  1:386935/1‑150 (MQ=255)

                                                                      tcatcaGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGgcg                                                                            >  2:3492420/1‑150 (MQ=255)

                                                                        atcaGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGcgcg                                                                          >  1:2805013/1‑150 (MQ=255)

                                                                         tcaGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCgg                                                                         <  2:767742/150‑1 (MQ=255)

                                                                         tcaGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCgg                                                                         >  2:451721/1‑150 (MQ=255)

                                                                         tcaGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCgg                                                                         <  2:1779216/150‑1 (MQ=255)

                                                                             gATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAAtt                                                                     <  2:2284572/150‑1 (MQ=255)

                                                                              aTAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAAttt                                                                    <  2:170292/150‑1 (MQ=255)

                                                                              aTAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAAttt                                                                    >  1:2485067/1‑150 (MQ=255)

                                                                              aTAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAAttt                                                                    >  2:3711226/1‑150 (MQ=255)

                                                                              aTAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAAttt                                                                    <  2:340915/150‑1 (MQ=255)

                                                                               tAAATCATAGTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTg                                                                   <  2:3041301/150‑1 (MQ=255)

                                                                                aaaTCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGc                                                                  >  2:2765833/1‑150 (MQ=255)

                                                                                aaaTCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGc                                                                  >  1:3941774/1‑150 (MQ=255)

                                                                                 aaTCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCt                                                                 >  1:4105709/1‑150 (MQ=255)

                                                                                    cATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTc                                                              <  2:1169274/150‑1 (MQ=255)

                                                                                     atatTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCa                                                             <  1:374283/150‑1 (MQ=255)

                                                                                       atTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACg                                                           >  2:1465486/1‑150 (MQ=255)

                                                                                       atTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACg                                                           <  1:2849588/150‑1 (MQ=255)

                                                                                       atTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACg                                                           <  1:1453377/150‑1 (MQ=255)

                                                                                          gtgtCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTc                                                        >  1:2972616/1‑150 (MQ=255)

                                                                                            gtCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTcac                                                      <  2:602769/150‑1 (MQ=255)

                                                                                             tCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTcacc                                                     >  2:1213579/1‑150 (MQ=255)

                                                                                                     tCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACataaat                                             <  2:3241311/150‑1 (MQ=255)

                                                                                                      cAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACataaata                                            >  2:673832/1‑150 (MQ=255)

                                                                                                      cAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACataaata                                            >  2:3639724/1‑150 (MQ=255)

                                                                                                         aaaCGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAataata                                         >  1:2090804/1‑150 (MQ=255)

                                                                                                           aCGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATaaa                                       <  1:942132/150‑1 (MQ=255)

                                                                                                            cGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATaaaa                                      <  1:626954/150‑1 (MQ=255)

                                                                                                            cGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATaaaa                                      <  1:2403389/150‑1 (MQ=255)

                                                                                                             gCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAAc                                     >  1:1931848/1‑150 (MQ=255)

                                                                                                              cAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACGTGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACg                                    >  1:3434716/1‑150 (MQ=255)

                                                                                                              cAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACg                                    <  1:3836231/150‑1 (MQ=255)

                                                                                                                aGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCa                                  <  2:678611/150‑1 (MQ=255)

                                                                                                                 gATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCaa                                 >  2:1707731/1‑150 (MQ=255)

                                                                                                                 gATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCaa                                 <  1:2189184/150‑1 (MQ=255)

                                                                                                                  aTCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCaaa                                <  1:516961/150‑1 (MQ=255)

                                                                                                                     tGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAAtgt                             <  2:2687046/150‑1 (MQ=255)

                                                                                                                      gcgcCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAAtgtg                            <  2:282457/150‑1 (MQ=255)

                                                                                                                          cAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCggg                        <  1:3385766/150‑1 (MQ=255)

                                                                                                                           aGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGgc                       >  2:4307867/1‑150 (MQ=255)

                                                                                                                           aGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGgc                       >  2:4308726/1‑150 (MQ=255)

                                                                                                                            gACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGgcg                      <  1:2077549/150‑1 (MQ=255)

                                                                                                                              cTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGgcgat                    <  2:844065/150‑3 (MQ=255)

                                                                                                                               ttGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTa                   <  2:4372000/150‑1 (MQ=255)

                                                                                                                                tGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTaa                  <  1:3161134/150‑1 (MQ=255)

                                                                                                                                  cGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAgg                >  1:2120496/1‑150 (MQ=255)

                                                                                                                                      tACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAg            <  1:342862/150‑1 (MQ=255)

                                                                                                                                      tACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAg            <  1:3280541/150‑1 (MQ=255)

                                                                                                                                      tACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAg            <  1:4285072/150‑1 (MQ=255)

                                                                                                                                          ccAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACg        >  1:4096487/1‑150 (MQ=255)

                                                                                                                                           cAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGACTGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGt       >  2:3101936/1‑150 (MQ=255)

                                                                                                                                           cAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGt       >  2:3102536/1‑150 (MQ=255)

                                                                                                                                            aGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTa      <  1:2508378/150‑1 (MQ=255)

                                                                                                                                               gggATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAAt   >  2:4326564/1‑150 (MQ=255)

                                                                                                                                               gggATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAAt   >  2:3085470/1‑150 (MQ=255)

                                                                                                                                                ggATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATc  <  1:1162743/150‑1 (MQ=255)

TTTTTCATAACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTT‑TCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATC  >  CP009273/2370212‑2370504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTTTTCATAACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTT‑TCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGCTG  >  CP009273/2370212‑2370510

TTTTTCATAACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTT                                                                                                                                                        <  SRR19973076.3036353/150‑1 (MQ=60)

TTTTTCATAACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTT                                                                                                                                                        <  SRR19973076.4180787/150‑1 (MQ=60)

 TTTTCATAACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTT‑G                                                                                                                                                      >  SRR19973076.1945971/1‑150 (MQ=60)

 TTTTCATAACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTT‑G                                                                                                                                                      >  SRR19973076.1945970/1‑150 (MQ=60)

 TTTTCATAACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTT‑G                                                                                                                                                      >  SRR19973076.237189/1‑150 (MQ=60)

  TTTCATAACTAATGTCCTCGAAACCTTCGGAGCAGTCGTTCCATTAATCCGGTGCGTAATTGATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGACTCGCTAGATATGAGGCAGACTTGCGATTACGCCAGCGGGATTTgt                                                                                                                                                      <  SRR19973076.4374720/150‑3 (MQ=60)

  TTTCATAACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTgt                                                                                                                                                      >  SRR19973076.4027430/1‑148 (MQ=60)

  TTTCATAACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTgt                                                                                                                                                      >  SRR19973076.4027375/1‑148 (MQ=60)

     CATACCGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCC                                                                                                                                                   <  SRR19973076.4397893/150‑1 (MQ=60)

     CATAACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCC                                                                                                                                                   <  SRR19973076.4398124/150‑1 (MQ=60)

        AACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGAT                                                                                                                                                >  SRR19973076.2835727/1‑150 (MQ=60)

        AACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGAT                                                                                                                                                >  SRR19973076.545934/1‑150 (MQ=60)

         ACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATG                                                                                                                                               >  SRR19973076.3325635/1‑150 (MQ=60)

          CGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGC                                                                                                                                              <  SRR19973076.854593/150‑1 (MQ=60)

           GAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCG                                                                                                                                             >  SRR19973076.4382691/1‑150 (MQ=60)

           GAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCG                                                                                                                                             >  SRR19973076.4378452/1‑150 (MQ=60)

           GAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCG                                                                                                                                             >  SRR19973076.998008/1‑150 (MQ=60)

             ATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCG                                                                                                                                           <  SRR19973076.151525/150‑1 (MQ=60)

              TGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGT                                                                                                                                          >  SRR19973076.3784898/1‑150 (MQ=60)

               GTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTT                                                                                                                                         <  SRR19973076.2565472/150‑1 (MQ=60)

                TCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTA                                                                                                                                        <  SRR19973076.3685889/150‑1 (MQ=60)

                TCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTA                                                                                                                                        <  SRR19973076.3685860/150‑1 (MQ=60)

                   TCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGT                                                                                                                                     >  SRR19973076.96466/1‑150 (MQ=60)

                   TCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGT                                                                                                                                     >  SRR19973076.96550/1‑150 (MQ=60)

                   TCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGT                                                                                                                                     >  SRR19973076.96481/1‑150 (MQ=60)

                     GAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCC                                                                                                                                   <  SRR19973076.3004402/150‑1 (MQ=60)

                         CCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCG                                                                                                                               <  SRR19973076.1585623/150‑1 (MQ=60)

                                AGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAA                                                                                                                        <  SRR19973076.3767305/150‑1 (MQ=60)

                                AGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAA                                                                                                                        <  SRR19973076.1098774/150‑1 (MQ=60)

                                    GTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGAC                                                                                                                    <  SRR19973076.3359313/150‑1 (MQ=60)

                                    GTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGAC                                                                                                                    >  SRR19973076.32288/1‑150 (MQ=60)

                                    GTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGAC                                                                                                                    >  SRR19973076.32292/1‑150 (MQ=60)

                                      CGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACAT                                                                                                                  >  SRR19973076.2436480/1‑150 (MQ=60)

                                              TCAACCGGTGCGTAAAGCATTGCTTCATCATGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACTTGGCTGTCG                                                                                                          <  SRR19973076.3520415/150‑1 (MQ=60)

                                              TCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCG                                                                                                          <  SRR19973076.3520413/150‑1 (MQ=60)

                                              TCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCG                                                                                                          <  SRR19973076.3520353/150‑1 (MQ=60)

                                              TCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCG                                                                                                          <  SRR19973076.3520387/150‑1 (MQ=60)

                                              TCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCG                                                                                                          <  SRR19973076.1238618/150‑1 (MQ=60)

                                              TCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTAG                                                                                                          >  SRR19973076.3772934/1‑150 (MQ=60)

                                               CAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTT‑TCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGC                                                                                                        <  SRR19973076.434226/150‑1 (MQ=60)

                                                AACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGC                                                                                                        >  SRR19973076.3889935/1‑150 (MQ=60)

                                                       GCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGT                                                                                                 <  SRR19973076.2948151/150‑1 (MQ=60)

                                                         GTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTC                                                                                               >  SRR19973076.3433944/1‑150 (MQ=60)

                                                          TAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCC                                                                                              >  SRR19973076.907724/1‑150 (MQ=60)

                                                             AGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCC                                                                                           >  SRR19973076.2599034/1‑150 (MQ=60)

                                                                    CTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGG                                                                                    <  SRR19973076.1701298/150‑1 (MQ=60)

                                                                    CTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGG                                                                                    <  SRR19973076.1701322/150‑1 (MQ=60)

                                                                     TTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGC                                                                                   <  SRR19973076.2990800/150‑1 (MQ=60)

                                                                     TTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTT‑TCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCG                                                                                  >  SRR19973076.387551/1‑150 (MQ=60)

                                                                     TTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTT‑TCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCG                                                                                  >  SRR19973076.387481/1‑150 (MQ=60)

                                                                      TCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCG                                                                                  >  SRR19973076.1275390/1‑150 (MQ=60)

                                                                      TCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCG                                                                                  >  SRR19973076.4375667/1‑150 (MQ=60)

                                                                        ATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCG                                                                                >  SRR19973076.2809086/1‑150 (MQ=60)

                                                                        ATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCG                                                                                >  SRR19973076.2808908/1‑150 (MQ=60)

                                                                              ATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTT                                                                          >  SRR19973076.2119791/1‑150 (MQ=60)

                                                                              ATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTT                                                                          >  SRR19973076.2488681/1‑150 (MQ=60)

                                                                                AAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGC                                                                        >  SRR19973076.3947644/1‑150 (MQ=60)

                                                                                AAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGC                                                                        >  SRR19973076.3947587/1‑150 (MQ=60)

                                                                                AAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGC                                                                        >  SRR19973076.439299/1‑150 (MQ=60)

                                                                                AAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGC                                                                        >  SRR19973076.439060/1‑150 (MQ=60)

                                                                                 AATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCT                                                                       >  SRR19973076.2984559/1‑150 (MQ=60)

                                                                                 AATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCT                                                                       >  SRR19973076.4111764/1‑150 (MQ=60)

                                                                                 AATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCT                                                                       >  SRR19973076.4111759/1‑150 (MQ=60)

                                                                                 AATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCT                                                                       >  SRR19973076.388516/1‑150 (MQ=60)

                                                                                 AATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGATCCGCCGCCAGGGCGCGGAATTTGCT                                                                       >  SRR19973076.2881738/1‑150 (MQ=60)

                                                                                     ATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCA                                                                   <  SRR19973076.374869/150‑1 (MQ=60)

                                                                                      TATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCAC                                                                  <  SRR19973076.332214/150‑1 (MQ=60)

                                                                                       ATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACG                                                                 <  SRR19973076.1455507/150‑1 (MQ=60)

                                                                                       ATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACG                                                                 >  SRR19973076.1472312/1‑150 (MQ=60)

                                                                                       ATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACG                                                                 <  SRR19973076.2853712/150‑1 (MQ=60)

                                                                                       ATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACG                                                                 <  SRR19973076.2853640/150‑1 (MQ=60)

                                                                                          GTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTC                                                              >  SRR19973076.2976942/1‑150 (MQ=60)

                                                                                               CTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCAC                                                         <  SRR19973076.682328/150‑1 (MQ=60)

                                                                                               CTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTT‑TCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACA                                                        <  SRR19973076.3382642/150‑1 (MQ=60)

                                                                                                     TCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAAT                                                   <  SRR19973076.3429475/150‑1 (MQ=60)

                                                                                                       AGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAA                                                 >  SRR19973076.3363287/1‑150 (MQ=60)

                                                                                                       AGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAA                                                 >  SRR19973076.746605/1‑150 (MQ=60)

                                                                                                        GAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAAT                                                <  SRR19973076.4291166/150‑1 (MQ=60)

                                                                                                         AAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATA                                               >  SRR19973076.2953326/1‑150 (MQ=60)

                                                                                                         AAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATA                                               >  SRR19973076.1507846/1‑150 (MQ=60)

                                                                                                         AAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATA                                               >  SRR19973076.2093998/1‑150 (MQ=60)

                                                                                                         AAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATA                                               <  SRR19973076.2201505/150‑1 (MQ=60)

                                                                                                         AAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATA                                               >  SRR19973076.2093845/1‑150 (MQ=60)

                                                                                                         AAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATA                                               >  SRR19973076.2093782/1‑150 (MQ=60)

                                                                                                           ACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAA                                             <  SRR19973076.943524/150‑1 (MQ=60)

                                                                                                            CGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAA                                            <  SRR19973076.2406855/150‑1 (MQ=60)

                                                                                                            CGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAA                                            <  SRR19973076.2406856/150‑1 (MQ=60)

                                                                                                            CGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAA                                            <  SRR19973076.627915/150‑1 (MQ=60)

                                                                                                             GCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAAC                                           >  SRR19973076.1934642/1‑150 (MQ=60)

                                                                                                             GCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAAC                                           >  SRR19973076.1934591/1‑150 (MQ=60)

                                                                                                              CAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACGTGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACG                                          >  SRR19973076.3439691/1‑150 (MQ=60)

                                                                                                              CAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACG                                          <  SRR19973076.3841969/150‑1 (MQ=60)

                                                                                                              CAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACG                                          >  SRR19973076.3439568/1‑150 (MQ=60)

                                                                                                              CAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACG                                          >  SRR19973076.3439715/1‑150 (MQ=60)

                                                                                                              CAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACG                                          <  SRR19973076.3841872/150‑1 (MQ=60)

                                                                                                              CAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACG                                          <  SRR19973076.3841863/150‑1 (MQ=60)

                                                                                                              CAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACG                                          >  SRR19973076.274875/1‑150 (MQ=60)

                                                                                                                 GATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAA                                       <  SRR19973076.2192311/150‑1 (MQ=60)

                                                                                                                 GATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAA                                       <  SRR19973076.2192344/150‑1 (MQ=60)

                                                                                                                  ATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAA                                      <  SRR19973076.517791/150‑1 (MQ=60)

                                                                                                                          CAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGG                              <  SRR19973076.3390671/150‑1 (MQ=60)

                                                                                                                          CAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGG                              <  SRR19973076.3390814/150‑1 (MQ=60)

                                                                                                                           AGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGC                             >  SRR19973076.2874134/1‑150 (MQ=60)

                                                                                                                            GACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCG                            <  SRR19973076.2080601/150‑1 (MQ=60)

                                                                                                                            GACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCG                            <  SRR19973076.2080573/150‑1 (MQ=60)

                                                                                                                            GACTTGCGATTACGCCAGCGGGATTT‑TCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGC                           <  SRR19973076.2529949/150‑1 (MQ=60)

                                                                                                                                TGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAACCGCAAATGTGCGGGCGCTAA                        <  SRR19973076.3165582/150‑1 (MQ=60)

                                                                                                                                TGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAA                        <  SRR19973076.3165719/150‑1 (MQ=60)

                                                                                                                                 GCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAG                       <  SRR19973076.3831137/150‑1 (MQ=60)

                                                                                                                                  CGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGG                      >  SRR19973076.4370268/1‑150 (MQ=60)

                                                                                                                                  CGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGG                      >  SRR19973076.4015626/1‑150 (MQ=60)

                                                                                                                                  CGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGG                      >  SRR19973076.2123574/1‑150 (MQ=60)

                                                                                                                                   GATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGT                     <  SRR19973076.4544846/150‑1 (MQ=60)

                                                                                                                                   GATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGT                     <  SRR19973076.1573488/150‑1 (MQ=60)

                                                                                                                                     TTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTA                   <  SRR19973076.519660/150‑1 (MQ=60)

                                                                                                                                      TACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAG                  <  SRR19973076.343390/150‑1 (MQ=60)

                                                                                                                                      TACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAG                  <  SRR19973076.4291403/150‑1 (MQ=60)

                                                                                                                                      TACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAG                  <  SRR19973076.3285301/150‑1 (MQ=60)

                                                                                                                                          CCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACG              >  SRR19973076.4100833/1‑150 (MQ=60)

                                                                                                                                          CCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACG              >  SRR19973076.4102529/1‑150 (MQ=60)

                                                                                                                                            AGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTA            <  SRR19973076.2512022/150‑1 (MQ=60)

                                                                                                                                            AGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTA            <  SRR19973076.2512016/150‑1 (MQ=60)

                                                                                                                                            AGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTA            <  SRR19973076.2511622/150‑1 (MQ=60)

                                                                                                                                                GGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATC        <  SRR19973076.1164466/150‑1 (MQ=60)

                                                                                                                                                GGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATC        <  SRR19973076.1164411/150‑1 (MQ=60)

                                                                                                                                                   TTG‑TCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGC    <  SRR19973076.3522422/150‑1 (MQ=60)

                                                                                                                                                   TTG‑TCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGC    <  SRR19973076.3522454/150‑1 (MQ=60)

                                                                                                                                                    TG‑TCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGAGGGCGCTAAGGTTAGCACGTAAATCAGGCT   >  SRR19973076.686890/1‑150 (MQ=60)

                                                                                                                                                     G‑TCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGCTG  <  SRR19973076.3327414/150‑1 (MQ=60)

TTTTTCATAACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTT‑TCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGCTG  >  CP009273/2370212‑2370510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`