Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F342 I0 R1	1331	225.9	7641038	95.8%	7320114	147.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,370,361:1	+G	100%	coding (711/759 nt)	menH ←	2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,370,361	1	.	G	99.6%	911.3 / ‑3.0	223	coding (711/759 nt)	menH	2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase
Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (107/115); total (108/115)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 231 total aligned reads displayed.

TTCATAACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTT‑TCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAA  >  CP009273/2370215‑2370502

ttCATAACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑Atttg                                                                                                                                               <  1:3509258/148‑2 (MQ=255)

 tCATAACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑Atttgt                                                                                                                                              <  2:2917232/148‑3 (MQ=255)

   aTAACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑Atttgtcc                                                                                                                                            >  2:2359522/1‑144 (MQ=255)

   aTAACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTAAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATAATGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATAACGCCAGCGGG‑Atttgtcc                                                                                                                                            >  1:2621099/1‑144 (MQ=255)

      aCGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGa                                                                                                                                         <  1:107128/148‑1 (MQ=255)

      aCGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGa                                                                                                                                         <  1:2979903/148‑1 (MQ=255)

      aCGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGa                                                                                                                                         <  1:2608973/148‑1 (MQ=255)

       cGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGAATTG‑TCCCGAt                                                                                                                                        >  2:1864969/1‑148 (MQ=255)

          aTGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATgcg                                                                                                                                     >  2:3069683/1‑148 (MQ=255)

           tGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATgcgc                                                                                                                                    >  2:2269675/1‑148 (MQ=255)

            gTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGcgcg                                                                                                                                   <  2:1274477/148‑1 (MQ=255)

             tCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGt                                                                                                                                  >  2:414802/1‑148 (MQ=255)

             tCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGt                                                                                                                                  >  1:3765976/1‑148 (MQ=255)

                tCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACTCCAGCGGG‑ATTTGTCCCGATGCGCGTTAt                                                                                                                               <  2:2609142/148‑1 (MQ=255)

                tCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTAt                                                                                                                               <  2:1776106/148‑1 (MQ=255)

                  gAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGt                                                                                                                             <  1:1946567/148‑1 (MQ=255)

                  gAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGt                                                                                                                             <  2:3048623/148‑1 (MQ=255)

                    aaCCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTcc                                                                                                                           >  1:420159/1‑148 (MQ=255)

                      ccTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCgg                                                                                                                         >  1:868724/1‑148 (MQ=255)

                       cTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGgc                                                                                                                        >  2:320141/1‑148 (MQ=255)

                              gCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGa                                                                                                                 <  2:711431/148‑1 (MQ=255)

                               cAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGaa                                                                                                                <  2:2982982/148‑1 (MQ=255)

                                  tCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGa                                                                                                             <  2:1432079/148‑1 (MQ=255)

                                       ccATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATgg                                                                                                        <  2:1456897/148‑1 (MQ=255)

                                           tCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGt                                                                                                    <  2:1017500/148‑1 (MQ=255)

                                           tCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGt                                                                                                    >  1:3099089/1‑148 (MQ=255)

                                            cAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTc                                                                                                   <  1:3661061/148‑1 (MQ=255)

                                               ccGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGc                                                                                                >  1:2682059/1‑148 (MQ=255)

                                                   tGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGcc                                                                                            >  2:940872/1‑148 (MQ=255)

                                                      gTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGt                                                                                         <  1:1467488/148‑1 (MQ=255)

                                                       tAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGtt                                                                                        <  2:3794011/148‑1 (MQ=255)

                                                        aaaGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTc                                                                                       <  1:1846991/148‑1 (MQ=255)

                                                         aaGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTcc                                                                                      >  1:2201992/1‑148 (MQ=255)

                                                              ttGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCcgccgc                                                                                 >  1:245191/1‑148 (MQ=255)

                                                               tGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCgccgcc                                                                                >  1:2074008/1‑148 (MQ=255)

                                                                 cTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGACTGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAg                                                                              >  2:1731705/1‑148 (MQ=255)

                                                                 cTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAg                                                                              >  2:3450799/1‑148 (MQ=255)

                                                                 cTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAg                                                                              >  2:1024233/1‑148 (MQ=255)

                                                                 cTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAg                                                                              >  2:1024531/1‑148 (MQ=255)

                                                                     atcaGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGgcg                                                                          >  2:3283609/1‑148 (MQ=255)

                                                                      tcaGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGgcgc                                                                         >  1:2757688/1‑148 (MQ=255)

                                                                         ggATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGa                                                                      <  1:3199790/148‑1 (MQ=255)

                                                                           aTAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAAt                                                                    >  1:133769/1‑148 (MQ=255)

                                                                            tAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAAtt                                                                   <  1:3811934/148‑1 (MQ=255)

                                                                            tAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAAtt                                                                   >  1:2680134/1‑148 (MQ=255)

                                                                              aaTCATAGTGTGTCCTTTAGACAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTg                                                                 <  1:306238/148‑1 (MQ=255)

                                                                               aTCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGc                                                                >  2:903917/1‑148 (MQ=255)

                                                                                  atatTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGt                                                             <  1:1088530/148‑1 (MQ=255)

                                                                                  atatTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGt                                                             <  2:3159931/148‑1 (MQ=255)

                                                                                   tatTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTc                                                            <  2:688802/148‑1 (MQ=255)

                                                                                    atTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCa                                                           >  2:1904085/1‑148 (MQ=255)

                                                                                      tgtgTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACg                                                         <  1:2297590/148‑1 (MQ=255)

                                                                                       gtgtCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGt                                                        <  1:3442283/148‑1 (MQ=255)

                                                                                         gtCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTc                                                      <  1:1373793/148‑1 (MQ=255)

                                                                                         gtCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAAAGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTc                                                      >  2:317152/1‑148 (MQ=255)

                                                                                           ccTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTcac                                                    >  2:1489841/1‑148 (MQ=255)

                                                                                           ccTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTcac                                                    >  1:3469071/1‑148 (MQ=255)

                                                                                           ccTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTcac                                                    >  2:3103552/1‑148 (MQ=255)

                                                                                           ccTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTcac                                                    >  2:608642/1‑148 (MQ=255)

                                                                                              ttAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTcaccac                                                 <  2:245794/148‑1 (MQ=255)

                                                                                              ttAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTcaccac                                                 >  2:3345630/1‑148 (MQ=255)

                                                                                              ttAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTcaccac                                                 >  2:606340/1‑148 (MQ=255)

                                                                                                 gTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACata                                              >  2:2192540/1‑148 (MQ=255)

                                                                                                  tCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACataa                                             <  2:2464050/148‑1 (MQ=255)

                                                                                                     gAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCGGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACataaata                                          <  1:3197514/148‑1 (MQ=255)

                                                                                                     gAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACataaata                                          >  2:3421135/1‑148 (MQ=255)

                                                                                                     gAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACataaata                                          >  1:1948327/1‑148 (MQ=255)

                                                                                                     gAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACataaata                                          <  1:550511/148‑1 (MQ=255)

                                                                                                     gAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACataaata                                          <  1:2469670/148‑1 (MQ=255)

                                                                                                     gAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACataaata                                          >  1:3232376/1‑148 (MQ=255)

                                                                                                      aaaCGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACataaataa                                         <  1:3062456/148‑1 (MQ=255)

                                                                                                      aaaCGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACataaataa                                         <  2:3765921/148‑1 (MQ=255)

                                                                                                      aaaCGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACataaataa                                         <  2:3215705/148‑1 (MQ=255)

                                                                                                        aCGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAataata                                       >  2:2717554/1‑148 (MQ=255)

                                                                                                           cAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATaaaa                                    <  2:3127324/148‑1 (MQ=255)

                                                                                                           cAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATaaaa                                    <  2:1369359/148‑1 (MQ=255)

                                                                                                           cAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATaaaa                                    <  2:3513388/148‑1 (MQ=255)

                                                                                                           cAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATaaaa                                    >  1:1401393/1‑148 (MQ=255)

                                                                                                            aaGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAAc                                   >  2:2020442/1‑148 (MQ=255)

                                                                                                            aaGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAAc                                   >  1:2838671/1‑148 (MQ=255)

                                                                                                              gATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGc                                 >  1:3053210/1‑148 (MQ=255)

                                                                                                                tCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCaa                               <  1:608642/148‑1 (MQ=255)

                                                                                                                 cTGCGCCAGACTTTCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGATGAATGACATGGCAGTCGGCTGCCAGTTCCGCCGCCAGGGCGCGGATTTTGCTGTCAAGTTCACCACATAATTAATAAAACGCaaa                              <  1:687492/148‑1 (MQ=255)

                                                                                                                   gcgcCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAAtg                            <  2:3671778/148‑1 (MQ=255)

                                                                                                                   gcgcCAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAAtg                            <  1:3702863/148‑1 (MQ=255)

                                                                                                                       cAGACTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCg                        <  2:795576/148‑1 (MQ=255)

                                                                                                                           cTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGgcg                    <  2:167256/148‑1 (MQ=255)

                                                                                                                           cTTGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGgcg                    >  2:662654/1‑148 (MQ=255)

                                                                                                                            ttGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGgcgc                   <  1:3036828/148‑1 (MQ=255)

                                                                                                                            ttGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTAACGTTCACCACATAAATAATAAAACGCAAATGTGCGGgcgc                   >  2:3313055/1‑148 (MQ=255)

                                                                                                                             tGCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCt                  >  1:1609478/1‑148 (MQ=255)

                                                                                                                              gCGATTACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTa                 <  2:715754/148‑1 (MQ=255)

                                                                                                                                  ttACGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGt             <  2:2682059/148‑1 (MQ=255)

                                                                                                                                    aCGCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTa           <  1:1864993/148‑1 (MQ=255)

                                                                                                                                      gCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGc         <  1:940872/148‑1 (MQ=255)

                                                                                                                                      gCCAGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGc         <  2:2201992/148‑1 (MQ=255)

                                                                                                                                         aGCGGG‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACg      <  1:1112691/148‑1 (MQ=255)

                                                                                                                                            ggg‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTaa   <  1:1045287/148‑1 (MQ=255)

                                                                                                                                             gg‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTaaa  >  2:1350115/1‑148 (MQ=255)

                                                                                                                                             gg‑ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTaaa  >  2:1722005/1‑148 (MQ=255)

TTCATAACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGG‑ATTT‑TCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAA  >  CP009273/2370215‑2370502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTTTTCATAACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTT‑TCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGCTG  >  CP009273/2370212‑2370510

TTTTTCATAACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTT                                                                                                                                                        <  SRR19973069.2294503/150‑1 (MQ=60)

TTTTTCATAACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTT                                                                                                                                                        <  SRR19973069.2531339/150‑1 (MQ=60)

TTTTTCATAACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTT                                                                                                                                                        <  SRR19973069.2531676/150‑1 (MQ=60)

 TTTTCATAACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTT‑G                                                                                                                                                      <  SRR19973069.3547475/150‑1 (MQ=60)

   TTCATAACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTgtc                                                                                                                                                     <  SRR19973069.1625082/150‑4 (MQ=60)

   TTCATAACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTgtc                                                                                                                                                     <  SRR19973069.3531848/150‑4 (MQ=60)

    TCATAACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTAAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATAATGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATAACGCCAGCGGGATTTGTCC                                                                                                                                                    >  SRR19973069.2638622/1‑150 (MQ=60)

     CATAACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATGCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCC                                                                                                                                                   >  SRR19973069.3648584/1‑150 (MQ=60)

         ACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATG                                                                                                                                               <  SRR19973069.2626460/150‑1 (MQ=60)

         ACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATG                                                                                                                                               <  SRR19973069.2626413/150‑1 (MQ=60)

         ACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATG                                                                                                                                               <  SRR19973069.108184/150‑1 (MQ=60)

         ACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATG                                                                                                                                               <  SRR19973069.2999686/150‑1 (MQ=60)

         ACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATG                                                                                                                                               <  SRR19973069.2050983/150‑1 (MQ=60)

              TGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGT                                                                                                                                          >  SRR19973069.3790453/1‑150 (MQ=60)

              TGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGT                                                                                                                                          >  SRR19973069.3790398/1‑150 (MQ=60)

                  CTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATG                                                                                                                                      <  SRR19973069.1062143/150‑1 (MQ=60)

                     GAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCC                                                                                                                                   <  SRR19973069.1960005/150‑1 (MQ=60)

                     GAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCC                                                                                                                                   <  SRR19973069.1960012/150‑1 (MQ=60)

                     GAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCC                                                                                                                                   >  SRR19973069.423223/1‑150 (MQ=60)

                     GAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCC                                                                                                                                   >  SRR19973069.423241/1‑150 (MQ=60)

                       AACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGG                                                                                                                                 >  SRR19973069.1363907/1‑150 (MQ=60)

                       AACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGG                                                                                                                                 >  SRR19973069.874777/1‑150 (MQ=60)

                                 GCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAAT                                                                                                                       <  SRR19973069.1083430/150‑1 (MQ=60)

                                  CAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATG                                                                                                                      >  SRR19973069.3121840/1‑150 (MQ=60)

                                   AGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGA                                                                                                                     <  SRR19973069.2290941/150‑1 (MQ=60)

                                          CCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCA                                                                                                              <  SRR19973069.3364991/150‑1 (MQ=60)

                                            ATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGT                                                                                                            >  SRR19973069.3119506/1‑150 (MQ=60)

                                               CAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGG                                                                                                         >  SRR19973069.140656/1‑150 (MQ=60)

                                               CAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGG                                                                                                         <  SRR19973069.3685029/150‑1 (MQ=60)

                                                AACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGC                                                                                                        >  SRR19973069.2700037/1‑150 (MQ=60)

                                                         GTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTC                                                                                               <  SRR19973069.1477724/150‑1 (MQ=60)

                                                         GTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTC                                                                                               >  SRR19973069.997225/1‑150 (MQ=60)

                                                          TAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCC                                                                                              >  SRR19973069.2216934/1‑150 (MQ=60)

                                                          TAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCC                                                                                              >  SRR19973069.1866195/1‑150 (MQ=60)

                                                           AAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCG                                                                                             <  SRR19973069.1859896/150‑1 (MQ=60)

                                                               CATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGC                                                                                         >  SRR19973069.247012/1‑150 (MQ=60)

                                                                ATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCC                                                                                        >  SRR19973069.2088369/1‑150 (MQ=60)

                                                                    CTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGG                                                                                    >  SRR19973069.2885481/1‑150 (MQ=60)

                                                                       CATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGC                                                                                 >  SRR19973069.2776097/1‑150 (MQ=60)

                                                                        ATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCG                                                                                >  SRR19973069.2480041/1‑150 (MQ=60)

                                                                        ATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCG                                                                                >  SRR19973069.3205138/1‑150 (MQ=60)

                                                                           AGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAC                                                                             >  SRR19973069.2544399/1‑150 (MQ=60)

                                                                            GGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAAT                                                                            >  SRR19973069.135015/1‑150 (MQ=60)

                                                                            GGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAAT                                                                            <  SRR19973069.3220594/150‑1 (MQ=60)

                                                                            GGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAAT                                                                            <  SRR19973069.3221202/150‑1 (MQ=60)

                                                                             GATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATT                                                                           >  SRR19973069.2697714/1‑150 (MQ=60)

                                                                             GATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATT                                                                           >  SRR19973069.2698104/1‑150 (MQ=60)

                                                                             GATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATT                                                                           >  SRR19973069.2698161/1‑150 (MQ=60)

                                                                               TAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTG                                                                         <  SRR19973069.3836607/150‑1 (MQ=60)

                                                                                 AATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCT                                                                       <  SRR19973069.308295/150‑1 (MQ=60)

                                                                                 AATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCT                                                                       <  SRR19973069.308300/150‑1 (MQ=60)

                                                                                 AATCATAGTGTGTCCTTTAGACAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCT                                                                       <  SRR19973069.308299/150‑1 (MQ=60)

                                                                                     ATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCA                                                                   <  SRR19973069.3016651/150‑1 (MQ=60)

                                                                                     ATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCA                                                                   <  SRR19973069.1167877/150‑1 (MQ=60)

                                                                                     ATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCA                                                                   <  SRR19973069.1096132/150‑1 (MQ=60)

                                                                                     ATATTGTGTCCTTTAGTAAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCA                                                                   <  SRR19973069.3818175/150‑1 (MQ=60)

                                                                                      TATTGTGTTCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCAC                                                                  >  SRR19973069.1448120/1‑150 (MQ=60)

                                                                                      TATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCAC                                                                  >  SRR19973069.1915506/1‑150 (MQ=60)

                                                                                         TGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTT                                                               <  SRR19973069.2313198/150‑1 (MQ=60)

                                                                                          GTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTC                                                              >  SRR19973069.998125/1‑150 (MQ=60)

                                                                                          GTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTC                                                              <  SRR19973069.3464630/150‑1 (MQ=60)

                                                                                           TGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCTATTACTCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCA                                                             >  SRR19973069.267168/1‑150 (MQ=60)

                                                                                           TGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCA                                                             >  SRR19973069.3628187/1‑150 (MQ=60)

                                                                                            GTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCAC                                                            <  SRR19973069.1383367/150‑1 (MQ=60)

                                                                                            GTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCAC                                                            >  SRR19973069.3491509/1‑150 (MQ=60)

                                                                                                  TAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATA                                                      >  SRR19973069.3522339/1‑150 (MQ=60)

                                                                                                   AGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAA                                                     >  SRR19973069.3726280/1‑150 (MQ=60)

                                                                                                     TCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAAT                                                   <  SRR19973069.3361245/150‑1 (MQ=60)

                                                                                                      CAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGACTTTGCTGTCACGTTCACCACATAAATA                                                  >  SRR19973069.1961788/1‑150 (MQ=60)

                                                                                                      CAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATA                                                  >  SRR19973069.3253528/1‑150 (MQ=60)

                                                                                                       AGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAA                                                 >  SRR19973069.2472121/1‑150 (MQ=60)

                                                                                                        GAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCGGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAAT                                                <  SRR19973069.3218295/150‑1 (MQ=60)

                                                                                                        GAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAAT                                                <  SRR19973069.3218608/150‑1 (MQ=60)

                                                                                                        GAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAAT                                                <  SRR19973069.2486241/150‑1 (MQ=60)

                                                                                                        GAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAAT                                                <  SRR19973069.554423/150‑1 (MQ=60)

                                                                                                        GAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAAT                                                <  SRR19973069.554359/150‑1 (MQ=60)

                                                                                                         AAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATA                                               <  SRR19973069.3082709/150‑1 (MQ=60)

                                                                                                            CGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGACTTTGCTGTCACGTTCACCACATAAATAATAAAA                                            >  SRR19973069.1411167/1‑150 (MQ=60)

                                                                                                             GCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAAC                                           >  SRR19973069.2857406/1‑150 (MQ=60)

                                                                                                             GCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAAC                                           >  SRR19973069.2713651/1‑150 (MQ=60)

                                                                                                             GCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAAC                                           >  SRR19973069.3070259/1‑150 (MQ=60)

                                                                                                             GCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAAC                                           >  SRR19973069.2857080/1‑150 (MQ=60)

                                                                                                              CAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACG                                          >  SRR19973069.502993/1‑150 (MQ=60)

                                                                                                              CAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACG                                          >  SRR19973069.130257/1‑150 (MQ=60)

                                                                                                               AAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGC                                         >  SRR19973069.3073415/1‑150 (MQ=60)

                                                                                                               AAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGC                                         >  SRR19973069.3073281/1‑150 (MQ=60)

                                                                                                                   TCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGTGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAAT                                     <  SRR19973069.2055833/150‑1 (MQ=60)

                                                                                                                   TCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAAT                                     <  SRR19973069.1440861/150‑1 (MQ=60)

                                                                                                                   TCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAAT                                     <  SRR19973069.612623/150‑1 (MQ=60)

                                                                                                                   TCTGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAAT                                     <  SRR19973069.612724/150‑1 (MQ=60)

                                                                                                                    CTGCGCCAGACTTTCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGATGAATGACATGGCAGTCGGCTGCCAGTTCCGCCGCCAGGGCGCGGATTTTGCTGTCAAGTTCACCACATAATTAATAAAACGCAAATG                                    <  SRR19973069.692293/150‑1 (MQ=60)

                                                                                                                     TGCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGT                                   <  SRR19973069.2375551/150‑1 (MQ=60)

                                                                                                                      GCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTG                                  <  SRR19973069.3822049/150‑1 (MQ=60)

                                                                                                                      GCGCCAGACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTG                                  <  SRR19973069.3727062/150‑1 (MQ=60)

                                                                                                                             ACTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGC                           >  SRR19973069.2853767/1‑150 (MQ=60)

                                                                                                                              CTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCT                          >  SRR19973069.1620296/1‑150 (MQ=60)

                                                                                                                              CTTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCT                          >  SRR19973069.1619832/1‑150 (MQ=60)

                                                                                                                               TTGCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTA                         <  SRR19973069.3056954/150‑1 (MQ=60)

                                                                                                                                 GCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAG                       <  SRR19973069.946751/150‑1 (MQ=60)

                                                                                                                                 GCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAG                       <  SRR19973069.2125840/150‑1 (MQ=60)

                                                                                                                                 GCGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAG                       <  SRR19973069.747262/150‑1 (MQ=60)

                                                                                                                                  CGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACGTAAATAATAAAACGCAAATGTGCGGGCGCTAAGG                      <  SRR19973069.3490519/150‑1 (MQ=60)

                                                                                                                                  CGATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGG                      <  SRR19973069.3089965/150‑1 (MQ=60)

                                                                                                                                    ATTACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTT                    >  SRR19973069.664888/1‑150 (MQ=60)

                                                                                                                                       ACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGC                 <  SRR19973069.1877973/150‑1 (MQ=60)

                                                                                                                                       ACGCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGC                 <  SRR19973069.1877997/150‑1 (MQ=60)

                                                                                                                                         GCCAGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCAC               <  SRR19973069.947226/150‑1 (MQ=60)

                                                                                                                                            AGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTA            <  SRR19973069.1120452/150‑1 (MQ=60)

                                                                                                                                            AGCGGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTA            <  SRR19973069.1120235/150‑1 (MQ=60)

                                                                                                                                               GGGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAAT         <  SRR19973069.1052548/150‑1 (MQ=60)

                                                                                                                                                GGATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATC        >  SRR19973069.64557/1‑150 (MQ=60)

                                                                                                                                                  ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAG      <  SRR19973069.3305119/150‑1 (MQ=60)

                                                                                                                                                  ATTTGTCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAG      <  SRR19973069.3306235/150‑1 (MQ=60)

                                                                                                                                                    TG‑TCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGCT   <  SRR19973069.391345/150‑1 (MQ=60)

                                                                                                                                                    TG‑TCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCCGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGCT   <  SRR19973069.391315/150‑1 (MQ=60)

                                                                                                                                                     G‑TCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGCTG  >  SRR19973069.709303/1‑150 (MQ=60)

                                                                                                                                                     G‑TCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGCTG  >  SRR19973069.709146/1‑150 (MQ=60)

                                                                                                                                                     G‑TCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGGAAATCAGGCTG  >  SRR19973069.709297/1‑150 (MQ=60)

TTTTTCATAACGAATGTCCTCGAAACCTTCGGAGCAGTCGTGCCATTCAACCGGTGCGTAAAGCATTGCTTCATCAGGATAAATCATATTGTGTCCTTTAGTCAGAAACGCAAGATCTGCGCCAGACTTGCGATTACGCCAGCGGGATTT‑TCCCGATGCGCGTTATGTCCGGCGCGAGGAATGACATGGCAGTCGGCAGCCAGTTCCGCCGCCAGGGCGCGGAATTTGCTGTCACGTTCACCACATAAATAATAAAACGCAAATGTGCGGGCGCTAAGGTTAGCACGTAAATCAGGCTG  >  CP009273/2370212‑2370510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`